• The Journal of Internal Korean Medicine
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• v.42 no.3
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• pp.351-374
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• 2021

Objectives: This study investigates the mechanism of Hwanggeum-tang (HGT) and Gamchosasim-tang (GST) on inflammatory bowel disease (IBD). Methods: The mice (C57BL/6N) were treated with distilled water and 3% dextran sulfate sodium (DSS) to experimentally induce ulcerative colitis. The mice were divided into 7 groups of (6 mice: normal, negative control, positive control (with sulfasalazine), 4 experimental groups (with HGT and GST, respectively). RAW 264.7 cells were used for cell experiments. The experiment was conducted in two ways: in vitro and in vivo. Results: In the experimental group (HGT, GST) of in vitro experiments, NO production decreased, and significant changes in gene expression and protein activation were observed. The length of the colon recovered in the experimental groups (HGT, GST) of the in vivo experiment was longer than that of the negative control group, and the mucosal barrier was recovered. Sone significant changes in the amount of mRNA expression were partially observed, and significant changes in protein activation also were confirmed. Conclusions: HGT and GST are effective in treating IBD caused by DSS. In the same herbal preparation group, the higher the concentration, the better the experimental effect, and when the same concentration was tested, HGT was more effective than GST. Herbal medicine has a higher antioxidant effect than sulfasalazine, so it is also excellent for cell protection.

• Journal of Physiology & Pathology in Korean Medicine
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• v.25 no.4
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• pp.683-690
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• 2011

The object of this study was to observe the effects of Hwanggeum-tang (HGT) aqueous extracts on Streptozotocin-induced rat's diabetes and related complications. Three different dosages of HGT extracts were orally administered oncea day for 28 days from 3 weeks after Streptozotocin treatment (60 mg/kg, single intraperitoneally administered). All the rats were checked at 3 weeks after Streptozotocin treatment as follows. Changes on the body weight, blood glucose level, kidney weight, serum BUN and creatinine level, liver weight, serum AST and ALT level, serum LDL, HDL, triglyceride and total cholesterol level were observed with changes on the pancreatic MDA content and GSH content. The results were compared with a potent antioxidant silymarin 100 mg/kg in which the effects on Streptozotocin-induced diabetes and related complications were already confirmed. As results of Streptozotocin-injected diabetes and related complications, dramatical decreases on the body weight, increase of the kidney and liver weight, increase of serum BUN, creatinine, AST, ALT, LDL, triglyceride, total cholesterol level and decreases of serum HDL level were detected in streptozotocin control as compared with intact control. In addition, marked increases of pancreatic MDA content and decreases of GSH content were also detected in streptozotocin control as compared with intact control. However, these diabetes and related complications, and inhibition of antioxidant effects induced by Streptozotocin were inhibited by 28 days continuous treatment of 50, 100 and 200 mg/kg of HGT extracts in the present study. HGT have favorable effects on the diabetes and various diabetic complications. Therefore, more detail mechanism studies should be conducedin future with the efficacy tests of individual herbal composition of HGT and the screening of the biological active compounds in herbs.

• Journal of Sasang Constitutional Medicine
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• v.29 no.1
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• pp.1-13
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• 2017

Objectives The purpose of this study is to understand the influence of Daesihog-tang on Taeeum-in's patholoy and prescription in "Donguisusebowon" of Sasang Constitutional Medicine. Methods The pathologic condition in which Daesihog-tang used and the relevant herbs and prescriptions in "Sanghanron", "Donguibogam", "Dongmuyoogo haedong", "Chobongwon", and "Donguisusebowon". Results and Conclusions 1. Daesiho-tang in "Sanghanron" was used to the symptom of Yangmyung disease merging with Soyang disease. In "Donguibogam", its indications were mainly used to the severe fever-symptom of Yangmyung disease and expanded to Onbyeong and so on. 2. The concept of Daesiho-tang in "Donguibogam" was changed into Hwanggeumdaehwang-tang in "Chobongwon", which can be used to treat Onbyeong with heat-dysenteric diarrhea, and severe fever of Taeeum-in. 3. The notion of combination between Daehwang and hwanggeum used in "Dongmuyoogo haedong" and "Chobongwon" was connected to Chungmongsukgondamhwan or Jogakhwanggeumdaehwang-tang in "Chobongwon". Especially, Jogakhwanggeumdaehwang-tang for Yeokkigammo and severe constipation was formed while the concept of Onbyeong in Hwanggeumdaehwang-tang' merged with the usage of Jogak and Daehwang or Yisunggugo-hwan. 4. Jogakhwanggeumdaehwang-tang influenced on the usage of Jogakdaehwang-tang for Yangdok and Onbyeong, while the usage of Chungmongsukgondamhwan was not found in "Donguisusebowon". 5. As a result, Daesiho-tang may be presented without usages for a specific patholoy in "Donguisusebowon" because it indirectly influenced on the concept of Yangdok and Onbyeong of Taeeum-in, and the formation of Jogakdaehwang-tang in "Donguisusebowon".

• Journal of Physiology & Pathology in Korean Medicine
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• v.26 no.2
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• pp.147-154
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• 2012

Hwanggeum-tang (HGT) was recorded in Dongeuibogam as being able to treat Sogal whose concept had been applied to Diabetes Mellitus (DM). Advanced glycation end products (AGEs) play important roles in the development of diabetic complications such as atherosclerosis by eliciting inflammatory responses. In this study, we examined the suppressive effects of HGT against inflammation elicited by AGEs. AGEs treatment increased the expression of pro-inflammatory cytokine gene TNF-${\alpha}$; chemokines MCP-1, IP-10; pro-inflammatory cyclooxygenase COX-2 on the THP-1 cells. HGT had suppressed the expression of pro-inflammatory genes and protein levels in AGE-treated THP-1 cells. HGT had also decreased intracellular ROS production stimulated by AGEs. These results suggest that HGT has beneficial effects for the improvement diabetic vascular complication through suppressing inflammatory responses elicited by AGEs.

• Journal of Physiology & Pathology in Korean Medicine
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• v.34 no.2
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• pp.61-66
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• 2020

Epithelial-mesenchymal transition (EMT) is the process by which epithelial cells lose their characters and acquire the properties of mesenchymal cells. EMT has been reported to exert an essential role in embryonic development. Recently, EMT has emerged as a pivotal mechanism in the metastasis of cancer and the fibrosis of chronic diseases. In particular, EMT is drawing attention as a mechanism of renal fibrosis in chronic kidney diseases such as diabetic nephropathy. In this study, we developed an EMT model by treating TGF-β1 on the podocytes, which play a key role in the renal glomerular filtration. This study explored the effects of Hwanggeum-tang (HGT) recorded in Dongeuibogam as being able to be used for the treatment of Sogal whose concept had been applied to Diabetes Mellitus (DM), on the TGF-β1-induced podocyte EMT. HGT suppressed the expression of vimentin and α-SMA, the EMT marker, in the human podocytes stimulated by TGF-β1. However, HGT increased the expression of ZO-1 and nephrin. Interestingly, HGT selectively inhibited the mTOR pathway rather than the classical Smad pathway. HGT also activated the AMPK signaling. HGT's inhibitory effect on the podocyte EMT through regulation of the mTOR pathway was achieved through the activation of AMPK, which was confirmed by comparison with cells treated with compound C (CC), an inhibitor of AMPK signaling. In conclusion, HGT can be applied to the renal fibrosis by preventing TGF-β1-induced EMT of podocytes through AMPK activation and mTOR inhibition.

• Journal of Sasang Constitutional Medicine
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• v.29 no.3
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• pp.211-223
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• 2017

Objectives The purpose of this study is to understand the formation of Jogakdaehwang-tang(JD-T)'s patholoy and the compositional change of it in Sasang Constitutional Medicine(SCM). Methods The pathologic condition in which JD-T was used and the change of its composition was studied in SCM Results and Conclusions 1. Jogak and Daehwang in "Dongmuyoogo Haedong" were presented with Ungdam because of the common purpose flowing the energy of lung. Therapeutic method for exogenous Ungibyeong in "Dongyisusebowon sangchobongwon" was subdivided into Hangwul's treated by Ungdam, and the heat pattern featuring contagious cold and constipation, treated by Jogakhwanggeumdaehwang-tang(JHD-T). 2. JD-T in "Dongyisusebowon Gabogubon" was used for Yangdok and Onbyeong which are exogenous disease causing by heat. The composition of JD-T has been completed as it included Mahwang, Gilgyeong, and Seungma derived from prescriptions relevant with Yangdok. 3. JD-T in "Dongyisusebowon Sinchukbon(Sinchukbon)" was included in pathology of Ganyeol, and it substituted Galgeunhaegi-tang(GH-T) for Onbyeong, not for Yangdok, so Galgeun and Seungma became the main drug in JD-T. Galgeunseung-gitang(GS-T) was created for treating heat pattern owing to Daehwang, its main drug, and moreover Jogak's effect is too strong. Therefore, Mahwang, Hwanggeum and Gilgyeong was excluded from the composition of JD-T. 4. The origins of JD-T could be Yiseonggugo-hwan, Daesiho-tang, Jowiseungchung-tang, Heukno-hwan, and GH-T. The change of JD-T went for them throughout JHD-T to JD-T. JD-T in "Sinchukbon" consists of Galgeun and Daehwang which directly gets rid of severe heat from small intestine, and Jogak and Seungma which help to eliminate mild heat existing from small intestine throughout nose to flesh.

• Journal of Sasang Constitutional Medicine
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• v.19 no.1
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• pp.171-185
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• 2007

1. Objectvies This experimental study was designed to investigate the effect of cheongpesagan-tang extract on the obstruction of the lipase activity and weight, plasma, UCP1, 2 mRNA in db/db mouse. Material and Methods: The body weight loss, food intake, feeding efficiency ratio, weight of the internal organs (liver, kidney, epididymal fat, brown adipose tissue), plasma glucose, triglyceride, total cholesterol, white adipose tissue, adipocyte size distribution, expression of UCP1, 2 mRNA were measured in db/db mouse administered Cheongpesagan-tang extract for 6 weeks. These were then compared with those of control groups administered the diet. 2. Results 1) Inhibitory effect against lipase activity was Kilgyung(81.7%), Nabokja (73.1%), Seungma(73.0%), Daewhang (68.4%), Kalgeun (55.3%), Kobon(34.5%), Hwanggeum(4.2%). 2) In the sample group, the body weight was significantly decrease than that of control group. 3) In the sample group, the weight of epididymal fat showed significantly decrease than that of control group. 4) In the sample group, triglyceride showed significantly decrease than that of control group. 5) In the sample group, distribution of adipose tissue showed significantly larger than that of control group. 6) In the sample group, UCP1, 2 mRNA in BAT showed significantly increase than that of control group. 3. Conclusions These results show that cheongpesagan-tang has an effect on the treatment of obesity.