• Preventive Nutrition and Food Science
• /
• v.1 no.1
• /
• pp.117-120
• /
• 1996

To enhance cellulose hydrolysis f Humicola insolens cellulase (HIC) was empolyed with immobilized glucose isomerase(IGI). Optimun pH and temperature for HIC were 6.5 and 55$^{\circ}C$, respectively, whereas those for IGI were 7.0 and 60$^{\circ}C$, respectively. Optimun loading size of IGI was 200mg(130 untis) with 15units of HIC. Reaction conditions were determined to be as follows: 55$^{\circ}C$,pH 6.5, HIC 15 units and IGI 130 units. After 24h hydrolysis, more than 65% of avicel was converted to glucose and fructose; in contrast, the conversion ratio of control was 40%.

• The Korean Journal of Mycology
• /
• v.50 no.4
• /
• pp.311-318
• /
• 2022

Endophytic fungal strains were isolated from the leaves of two conifer species (Juniperus rigida and Pinus densiflora) in Korea and identified on the basis of their morphological and molecular characteristics. Internal transcribed spacer and large subunit regions of rDNA were used for the phylogenetic analysis, and the translation elongation factor 1-alpha (TEF) and RNA polymerase II second largest subunit (RPB2) genes were analyzed depending on the species. Two fungal species that were previously unrecorded in Korea were identified: Acrodontium burrowsianum and Pestalotiopsis humicola. Their morphological and phylogenetic characteristics are described herein.

• Mycobiology
• /
• v.29 no.2
• /
• pp.90-95
• /
• 2001

During the study of microbial structures in root-regions of tomato and red pepper from fields, various soil-inhabiting fungi were isolated with the dilution plate technique. Among them an ascomycete, Emericellopsis mirabilis and three hyphomycetes, Gliocladium solani, Humicola veronae and Verticillium chlamydosporium are presented for the first time in Korea along with Talaromyces trachyspermus, Chaetomium globosum and Doratomyces microsporus.

• The Plant Pathology Journal
• /
• v.26 no.3
• /
• pp.245-252
• /
• 2010

An endophytic bacterial strain, Arthrobacter humicola YC6002, was isolated from a surface sterilized root of Korean turf grass (Zoysia japonica) collected from Jinju, Korea. This strain showed inhibitory effect on germination and shoot growth of radish. The inhibition of germination and shoot growth of radish seeds varied depending on the age of culture and the temperature at which it was incubated. The culture filtrate of 1/10-strength Tryptic Soy Broth medium, incubated for 48 hours at $30^{\circ}C$, showed the highest inhibitory effect on radish seed germination and shoot growth (92% inhibition as compared to control). The active compound with seed germination and shoot growth inhibition was purified and identified as 3-phenylpropionic acid. The purified compound had 53% and 93% inhibitory effect on seed germination and shoot growth of radish for 500 and 1000 ppm solutions, respectively.

• The Korean Journal of Mycology
• /
• v.44 no.3
• /
• pp.184-187
• /
• 2016

Endophytic fungi were isolated from surface-sterilized roots of a species of orchid, Oreorchis patens, in Korea. The isolated strains were identified based on morphological characteristics and sequence analyses of the internal transcribed spacer (ITS) and large subunit (LSU) of rDNA regions. Two species, Thelonectria veuillotiana and Phialocephala humicola, are first reported in Korea with their descriptions.

• The Korean Journal of Mycology
• /
• v.48 no.3
• /
• pp.217-228
• /
• 2020

Endophytic fungi were isolated from the leaves of plants inhabiting the Hansando Island in Korea. We identified the fungal strains based on their morphological characteristics and on the phylogenetic analysis using DNA sequences of the internal transcribed spacer, small subunit rDNA, large subunit rDNA, and translation elongation factor 1- α regions. In this study, we isolated five endophytic fungi that have not been recorded in Korea, Arthrinium camelliaesinensis, Beltraniella humicola, B. portoricensis, Microxiphium theae, and Piceomphale pinicola. Their morphological characteristics as well as the results of molecular phylogenetic analysis are reported here.

• The Korean Journal of Mycology
• /
• v.46 no.2
• /
• pp.134-144
• /
• 2018

Three fungal strains belonging to the class Sordariomycetes, namely BH-06, 17-039 and BE12-1, were isolated from soils in Korea and identified as Chaunopycnis alba, Myrothecium cinctum and Humicola olivacea, respectively. These species were confirmed according to their morphological characteristics and phylogenetic relationships determined based on internal transcribed spacer regions, as well as large subunit, small subunit, ${\beta}$-Tubulin and RNA polymerase II largest subunit sequences of ribosomal DNA. These three species are the first members of the Sordariomycetes reported in Korea.

• Journal of Practical Agriculture & Fisheries Research
• /
• v.14 no.1
• /
• pp.61-83
• /
• 2012

To identification of fungi that occurs round bale silages, 253 fungal contaminated samples were collected from 2009 to 2011. Total 253 silage samples from Italian ryegrass, sudan grass, rye, corn, barley and oat were analysed. Total 270 strains were purely isolated from contaminated round bale silages. The fungi were identified with morphological characteristics and rDNA sequence analysis. Nineteen species of fungi(Rhizopus sp., Fusarium spp., Coprinus sp., Blastomyces sp., Aureobasidium sp., Polypaecilum sp., Botryoderma sp., Mucor sp., Scytalidium sp., Sphaeropsis sp., Aspergillus spp., Trichocladium sp., Humicola sp., Staphylotrichum sp., Periconia sp., Verticillium sp., Diplococcium sp., Penicillium spp. and Trichoderma spp.) were identified by morphological characteristics. On the other hand, fungi isolated from silage were identified to Acremonium strictum, Aspergillus tubingensis, Bionectria ochroleuca, Dipodascaceae sp., Fusarium proliferatum, Fusarium oxysporum, Fusrium solani, Gelasinospora reticulata, Gibberella moniliformis, Gibberella zeae, Nectria mauritiicola, Penicillium paneum, Pseudallecheria boydii, Schizophyllum commune, Scopulariopsis brevicaulis and Simplicillium lamellicola by rDNA sequence analysis. Penicillium sp. and Trichoderma sp., were isolated 74 and 64 strains, respectively. Humicola sp., Aspergillus sp., Coprinus sp., and Fusarium spp. were identified 10 to 30 strains. Most fungi were isolated together with more than one species in a sample looked like one species with the naked eyes.

• The Korean Journal of Mycology
• /
• v.43 no.1
• /
• pp.20-25
• /
• 2015

This study was conducted to investigate diversity of orchid endophytic fungi from roots of two terrestrial orchids, Cypripedium japonicum and Cypripedium macranthum in Korea. The endophytic fungi were identified using morphological and DNA sequences isolated from the roots. Totally, 11 taxa of the endophytic fungi were identified from the roots of C. japonicum and 15 taxa from C. macranthum. Three species of the fungi were common in both host species; Leptodontidium orchidicola, Humicola fuscoatra var. fuscoatra, Umbelopsis dimorpha. Six species of the fungal isolates were the first report in Korea; Oidiodendron echinulatum, Pseudogymnoascus roseus, Geomyces vinaceus, Cryptosporiopsis ericae, U. dimorpha, Chaetomium cupreum.

• Journal of Microbiology and Biotechnology
• /
• v.23 no.6
• /
• pp.794-801
• /
• 2013

This paper reports the production and characterization of crude xylanase from the newly isolated Humicola sp. Ly01. The highest (41.8 U/ml) production of the crude xylanase was obtained under the optimized conditions (w/v): 0.5% wheat bran, 0.2% $KH_2PO_4$, and 0.5% peptone; initial pH 7.0; incubation time 72 h; $30^{\circ}C$; and 150 rpm. A considerable amount of the crude xylanase was induced using hulless barley bran or soybean meal as the carbon source, but a small amount of the enzyme was produced when supplementary urea was used as the nitrogen source to wheat bran. The crude xylanase showed apparent optimal cellulase-free xylanase activity at $60^{\circ}C$ and pH 6.0, more than 71.8% of the maximum xylanase activity in 3.0-30.0% (v/v) ethanol and more than 82.3% of the initial xylanase activity after incubation in 3.0-30.0% (v/v) ethanol at $30^{\circ}C$ for 2 h. The crude xylanase was moderately resistant to both acid and neutral protease digestion, and released 7.9 and 10.9 ${\mu}mol/ml$ reducing sugar from xylan in the simulated gastric and intestinal fluids, respectively. The xylooligosaccharides were the main products of the hydrolysis of xylan by the crude xylanase. These properties suggested the potential of the crude enzyme for being applied in the animal feed industry, xylooligosaccharides production, and high-alcohol conditions such as ethanol production and brewing.