The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Diversity of Endophytic Fungi Isolated from Roots of Cypripedium japonicum and C. macranthum in Korea

광릉요강꽃과 복주머니란의 뿌리에서 분리한 내생균의 다양성

  • Lee, Bong-Hyung (Department of Biology Education, Korea National University of Education) ;
  • Han, Han-Kyeol (Department of Biology Education, Korea National University of Education) ;
  • Kwon, Hye-Jin (Plant Conservation Division, Korea National Arboretum) ;
  • Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education)
  • 이봉형 (한국교원대학교 생물교육과) ;
  • 한한결 (한국교원대학교 생물교육과) ;
  • 권혜진 (국립수목원 산림자원보존과) ;
  • 엄안흠 (한국교원대학교 생물교육과)
  • Received : 2015.03.02
  • Accepted : 2015.03.07
  • Published : 2015.03.31
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Abstract

This study was conducted to investigate diversity of orchid endophytic fungi from roots of two terrestrial orchids, Cypripedium japonicum and Cypripedium macranthum in Korea. The endophytic fungi were identified using morphological and DNA sequences isolated from the roots. Totally, 11 taxa of the endophytic fungi were identified from the roots of C. japonicum and 15 taxa from C. macranthum. Three species of the fungi were common in both host species; Leptodontidium orchidicola, Humicola fuscoatra var. fuscoatra, Umbelopsis dimorpha. Six species of the fungal isolates were the first report in Korea; Oidiodendron echinulatum, Pseudogymnoascus roseus, Geomyces vinaceus, Cryptosporiopsis ericae, U. dimorpha, Chaetomium cupreum.

본 연구에서는 경기도 포천과 강원도 춘천에 자생하는 광릉요강꽃과 강원도 삼척에서 자생하는 복주머니란의 뿌리에서 내생균을 순수 분리하였고, 분리된 균주의 ITS 부위의 염기서열을 이용하여 계통 분석하였다. 그 결과 광릉요강꽃에서는 11종의 균주가 분리되었고, 복주머니란에서는 15종의 균주가 분리되었으며, Leptodontidium orchidicola, Humicola fuscoatra var. fuscoatra, Umbelopsis dimorpha 등의 균주는 공통으로 분리되었다. 분리된 균주 중 Oidiodendron echinulatum, Pseudogymnoascus roseus, Geomyces vinaceus, Cryptosporiopsis ericae, Umbelopsis dimorpha, Chaetomium cupreum 은 국내 미기록종으로 확인하였다.

Keywords

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