• 한국가금학회지
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• 제33권4호
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• pp.317-321
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• 2006

본 연구는 humic substances의 급여가 산란계의 생산성과 난 특성에 미치는 영향을 알아보고자 실시하였다. 55주령 ISA brown 갈색계 252수를 공시하였고, 6주간 시험을 실시하였다. 처리구는 1) CON (basal diet),2) HS5 (basal diet added humic substances 5%), and 3) HS10 (basal diet added humic sudstances 10%)으로 처리구당 7반복 반복당 12수씩 완전 임의 배치하였다. 전 시험 기간 동안 hmic substances을 10% 첨가한 처리구는 난 생산성과 난황 직경이 대조구와 비교하여 유의적으로 감소하였다(P<0.05). 난황색은 HS10 처리구가 CON처리구와 비교하여 높게 나타났다(P<0.05). 개시시 난중은 처리구간에 차이가 없었다(P<0.05). 난각 강도에서는 HS5 처리구가 다른 처리구들과 비교하여 유의적으로 높게 나타났다(P<0.05). 결론적으로, 산란계 사료내 HS 5% 또는 10%의 첨가는 난황색 및 난각 강도를 향상시킬 수 있을 것으로 사료된다.

• 한국초지조사료학회지
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• 제38권3호
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• pp.175-179
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• 2018

Humic substances that can be obtained from coal resources such as leonardite in a bulk scale have been employed as crop stimulators and soil conditioners. The polymeric organics containing a variety of aromatic and aliphatic structures are known to activate plants in a multifunctional way, thus resulting in enhanced germination rate and abiotic stress resistance concomitant with induction of numerous genes and proteins. Although detailed structural-functional relationship of humic substances for plant stimulations has not been deciphered yet, cutting-edge analytical tools have unraveled critical features of humic architectures that could be linked to the action mechanisms of their plant stimulations. In this review article, we introduce key findings of humic structures and related biological functions that boost plant growth and abiotic stress resistance. Oxygen-based functional groups and plant hormone-like structures combined with labile and recalcitrant carbon backbones are believed to be critical moieties to induce plant stimulations. Some proteins such as HIGH-AFFINITY $K^+$ TRANSPORTER 1, phospholipase A2 and $H^+$-ATPase have been also recognized as key players that could be critically involved in humic substance-driven changes in plant physiology.

• 산업기술연구
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• 제31권B호
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• pp.127-132
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• 2011

Humic substances is accounted for for the largest proportion in natural organic matter(NOM) and NOM is widely distributed in varying concentration in all aquatic and soil. They can affect water quality adversely in several ways by contributing undesirable color, complexing with metal and yielding metal concentrations exceeding normal solubility. Ozonation is one of the efficient treatments for degradation of humic substances which cause some problems in water treatment. Especially, the combination of ozone and granular activated carbon was applied to degradation humic acid in aquatic system. The aim of this work to test the available of acid-treated granular activated carbon as catalyst in the ozonation of humic acid.

• 미생물학회지
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• 제34권3호
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• pp.132-136
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• 1998

자연환경의 미생물군집을 분자생태학적 방법으로 분석하기 위해서는 오염되지 않은 순수한 핵산의 분리가 필요하다. 해양퇴적물에서 핵산을 추출할 때 같이 추출되는 부식물질(humic substances)은 핵산중폭반응을 저해하기 때문에, 이를 제거하기 위한 네 가지의 방법, 아가로즈젤 전기영동후 용출, Sephadex G-75 젤, Hydroxyapatite 젤, 그리고 PVPP(poluvinylpolypyrrolidone) microspin column 방법에 대해 그 효율성을 비교하였다. 조산된 방법 중에서 PVPP microspin column을 이용하면 건조 퇴적물 1g당 $4.8{\mu}g$의 순수한 DNA를 신속하고 간편하게 얻을 수 있었고, 이 방법으로 분리된 DNA를 PCR의 주형으로 사용한 결과 16S rRNA 유전자의 거의 전 범위에 해당하는 1.5 kb 크기의 PCR 산물을 얻을 수 있었다.

• Membrane and Water Treatment
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• 제1권4호
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• pp.283-296
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• 2010

During the disinfection of potable water, humic substances present in the solution react with chlorine to form potential carcinogenic compounds. This study evaluates the feasibility of using a submerged membrane photocatalysis reactor (SMPR) process for treatment of humic substances through the characterization of both organic removal efficiency and membrane hydraulic performance. A simple SMPR was operated and led to the removal of up to 83% of the polluting humic matters. Temporal rates of organic removal and membrane fouling were found to decrease with filtration time. Using tighter membrane in the hybrid process resulted in not only higher organic removal, but also more significant membrane fouling. Under the experimental conditions tested, optimum $TiO_2$ concentration for humic removal was found to be 0.6 g/L, and increasing initial pollutant concentration expectedly resulted in a more substantial membrane fouling. The importance of the influent nature and pollutant characteristics in this type of treatment was also assessed as various water sources were tested (model humic acid solution vs. local water containing natural organic matters). Results from this study revealed the promising nature of the SMPR process as an alternative technique for organic removal in the existing water treatment system.

• Membrane and Water Treatment
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• 제9권5호
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• pp.353-361
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• 2018

Fouling characteristics of humic substances on tight ultrafiltration (UF) membrane have been investigated. The tight UF membrane was prepared by blending polysulfone (PSf) in N.N-dimethylacetamide (DMAc) with 25%wt of Polyethylene glycol (PEG400) and 4%wt of acetone. Fouling characteristic of the modified PSf membrane was observed during peat water filtration in different trans-membrane pressure (TMP). It was found that the acetone modified membrane provided 13% increase in TMP during five hours of peat water filtration, where a stable flux was reached within 150 minutes. Meanwhile, the increase of TMP from 10 psig to 30 psig resulted in a fouling resistance enhancement of 60%. Furthermore, based on the fouling analysis, fouling mechanism at the first phase of filtration was attributed to intermediate blocking while the second phase was cake formation.

• Bulletin of the Korean Chemical Society
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• 제14권1호
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• pp.72-78
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• 1993

The application of time-resolved laser induced fluorescence spectroscopy (TRLIF) to the complexation studies of Eu(III) and Cm(III) with humic substances is described. Using this method, three different spectroscopic characteristics(excitation spectra, emission spectra, and lifetimes) of these aquo ions and their complexes can be directly measured. By observing shifts in the wavelength and changes in the lifetime and intensities of the fluorescence emission, the information on the complexation behavior of humic substances with these trivalent metal cations in an aqueous solution, as well as energy transfer mechanisms, can be obtained. In addition, this method allows precise spectroscopic quantification of the complexation processes at very low concentrations of both components.

• 한국지하수토양환경학회:학술대회논문집
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• 한국지하수토양환경학회 2003년도 추계학술발표회
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• pp.123-127
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• 2003

Changes in pyrene partitioning due to mineral surface adsorptive fractionation processes of humic substances (HS) were examined in model environmental systems. For purified Aldrich humic acid(PAHA), carbon-normalized pyrene binding coefficients ( $K_{oc}$ ) for the residual (i.e., nonadsorbed and dissolved) PAHA components were different from the original dissolved PAHA $K_{oc}$ , value prior to contact with mineral suspensions. A positive correlation between the extent of pyrene binding and weight-average molecular weight (M $W_{w}$) of residual PAHA components was observed, which appeared to be unaffected by the specific mineral adsorbents use and fractionation mechanisms. A similar positive correlation was not observed with the adsorbed PAHA components, suggesting that conformational changes occurred for the mineral-associated components upon adsorption. Nonlinear pyrene sorption to mineral-associated PAHA was observed, and the degree of nonlinearity is hypothesized to be dependent on adsorptive fractionation effects and/or structural rearrangement of the adsorbed PAHA components.s.

• 미생물학회지
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• 제26권2호
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• pp.122-128
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• 1988

The enzymatic properties of soil cellobiohydrolase were examined and compared with those of cellobiohydrolase-active extracts from soil in the forms of enzyme-humic complex and humicfree enzyme, and cellobiohydrolase partially pruified from Aspergillus niger. The pH optima of soil cellobiohydrolase and cellobiohydrolase-humic complex were greater by 1.5-3.0 pH units than those of cellobiohydrolase in humic-free extract and from A. niger. Soil cellobiohydrolase and cellobiohydrolase-humic complex were remarkably resistant to thermal denaturation and proteolysis. These results confirm that cellobiohydrolase in soil is atable in conditions which rapidly inactivate microbial cellobiohydrolase and that its stability is due to the immobilization of this enzyme by association with humic substances. The Michaelis-Menten constants (Km) for soil, cellobiohydrolase-humic complex, humic free extract and cellobiohydrolase from A. niger were 22.1mg/ml, 11.3mg/ml, 10.6mg/ml and 4.5 mg/ml of Avicel, respectively.

• 대한환경공학회지
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• 제27권3호
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• pp.240-246
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• 2005

오존 및 입상활성탄 공정으로 구성된 고도정수처리시설을 대상으로 공정유입수인 모래여과수를 포함하는 각각의 공정수로부터 휴믹물질(HS; humic substances)을 분리 및 추출하여 작용기 분포, 화학적 조성, FT-IR(Fourier transform infrared) 그리고 $^1H$-NMR(proton nuclear magnetic resonance) 스펙트럼을 분석하여 구조 및 화학적 특성을 평가하였다. 오존처리에 이어 입상활성탄 흡착처리를 거치면서 휴믹성분(humic fraction)의 농도분포는 36.3%에서 24.2%로 단계적으로 감소하였으며, 모래여과수가 활성탄 흡착칼럼으로 직접 유입되는 공정의 경우 36.3%에서 25.0%로 감소하는 것으로 나타났다. 전오존처리 이후 활성탄 흡착처리시 HS 중 페놀기(phenolic groups)와 카르복실기(carboxylic groups)의 농도분포는 38.4%에서 23.5%로 그리고 61.6%에서 43.3%로 각각 감소하였으며, 전오존처리하지 않은 경우 38.4%에서 24.0%로 그리고 61.6%에서 44.9%로 각각 감소하였다. 정수처리를 거치면서 HS의 카르복실기에 대한 페놀기의 비율에 따라 HS 분자구조 중 O/C 몰비가 감소하거나 또는 증가하는 결과를 나타내었다.