• 한국정보통신학회논문지
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• 제16권7호
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• pp.1335-1343
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• 2012

대양을 항해하는 선박의 안전 운항을 확보하기 위해 선박 항해시스템 및 선박 자동식별 장치 등의 장비가 기존 선박에 운용되고 있으나, 해상 교통량 증가에 따른 선박 사고는 증가하는 추세에 있다. 이러한 선박 사고는 항해자 및 승무원 등의 인적 오류에 의한 운항과실이 주된 요인으로 파악되었으며 이에 따른 해양사고를 미연에 방지하기 위한 기술이 필요한 실정이다. 따라서 본 논문에서는 선박 내 항해 및 운항자의 안전 운항을 감시하고 체계적인 관리를 위한 승무원 운용상황 감시 및 관리 시스템을 제안하고, 운항 안전을 위한 통합감시 플랫폼 설계와 승무원 휴대 상태계측 장치 및 휴대 단말시스템을 구현하였다. 또한 이를 통해 통합 안전 정보들의 상호 운용 가능성을 확인할 수 있었다.

• The Korean Journal of Physiology and Pharmacology
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• 제18권2호
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• pp.163-168
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• 2014

Endothelial progenitor cells (EPCs) are known to play an important role in the repair of damaged blood vessels. We used an endothelial progenitor cell colony-forming assay (EPC-CFA) to determine whether EPC numbers could be increased in healthy individuals through regular exercise training. The number of functional EPCs obtained from human peripheral blood-derived AC133 stem cells was measured after a 28-day regular exercise training program. The number of total endothelial progenitor cell colony-forming units (EPC-CFU) was significantly increased compared to that in the control group (p=0.02, n=5). In addition, we observed a significant decrease in homocysteine levels followed by an increase in the number of EPC-CFUs (p=0.04, n=5), indicating that the 28-day regular exercise training could increase the number of EPC colonies and decrease homocysteine levels. Moreover, an inverse correlation was observed between small-endothelial progenitor cell colony-forming units (small-EPC-CFUs) and plasma homocysteine levels in healthy men (r=-0.8125, p=0.047). We found that regular exercise training could increase the number of EPC-CFUs and decrease homocysteine levels, thus decreasing the cardiovascular disease risk in men.

• 동의생리병리학회지
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• 제28권6호
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• pp.607-613
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• 2014

In a previous study, we have shown that mulberry leaves (Mori Folium) extract MFE) and its compounds have the antioxidant effect in human red blood cells. However, the possible effect of MFE and its compounds on improvement of blood flow were not reported. Therefore, the aim of this study is to investigate the effects of MFE and its compounds on improvement of blood flow in a rat model of topical ferric chloride ($FeCl_3$)-induced carotid artery damage. The $FeCl_3$ treatment seriously damaged the carotid artery: the walls of the artery, blood flow rate, blood vessel diameter, blood vessel area and blood flow amount. However, administration of MFE or its compound has ameliorated the blood flow and suppressed thrombus in blood vessels. Moreover, the concentrations of serum total cholesterol, triglycerides, and LDL cholesterol in the MET and its compound groups were remarkably reduced in comparison to the control group, and HDL cholesterol concentration was higher in the MET and its compound groups than in the control group. These results suggest that MFE and its compounds ameliorate the thrombosis against blood vessel damage.

• Biomolecules & Therapeutics
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• 제26권5호
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• pp.474-480
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• 2018

Most angiogenesis assays are performed using endothelial cells. However, blood vessels are composed of two cell types: endothelial cells and pericytes. Thus, co-culture of two vascular cells should be employed to evaluate angiogenic properties. Here, we developed an in vitro 3-dimensional angiogenesis assay system using spheroids formed by two human vascular precursors: endothelial colony forming cells (ECFCs) and mesenchymal stem cells (MSCs). ECFCs, MSCs, or ECFCs+MSCs were cultured to form spheroids. Sprout formation from each spheroid was observed for 24 h by real-time cell recorder. Sprout number and length were higher in ECFC+MSC spheroids than ECFC-only spheroids. No sprouts were observed in MSC-only spheroids. Sprout formation by ECFC spheroids was increased by treatment with vascular endothelial growth factor (VEGF) or combination of VEGF and fibroblast growth factor-2 (FGF-2). Interestingly, there was no further increase in sprout formation by ECFC+MSC spheroids in response to VEGF or VEGF+FGF-2, suggesting that MSCs stimulate sprout formation by ECFCs. Immuno-fluorescent labeling technique revealed that MSCs surrounded ECFC-mediated sprout structures. We tested vatalanib, VEGF inhibitor, using ECFC and ECFC+MSC spheroids. Vatalanib significantly inhibited sprout formation in both spheroids. Of note, the $IC_{50}$ of vatalanib in ECFC+MSC spheroids at 24 h was $4.0{\pm}0.40{\mu}M$, which are more correlated with the data of previous animal studies when compared with ECFC spheroids ($0.2{\pm}0.03{\mu}M$). These results suggest that ECFC+MSC spheroids generate physiologically relevant sprout structures composed of two types of vascular cells, and will be an effective pre-clinical in vitro assay model to evaluate pro- or anti-angiogenic property.

• Molecules and Cells
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• 제43권6호
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• pp.539-550
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• 2020

Glioblastoma multiforme (GBM) is a fatal malignant tumor that is characterized by diffusive growth of tumor cells into the surrounding brain parenchyma. However, the diffusive nature of GBM and its relationship with the tumor microenvironment (TME) is still unknown. Here, we investigated the interactions of GBM with the surrounding microenvironment in orthotopic xenograft animal models using two human glioma cell lines, U87 and LN229. The GBM cells in our model showed different features on the aspects of cell growth rate during their development, dispersive nature of glioma tumor cells along blood vessels, and invasion into the brain parenchyma. Our results indicated that these differences in the two models are in part due to differences in the expression of CXCR4 and STAT3, both of which play an important role in tumor progression. In addition, the GBM shows considerable accumulation of resident microglia and peripheral macrophages, but polarizes differently into tumor-supporting cells. These results suggest that the intrinsic factors of GBM and their interaction with the TME determine the diffusive nature and probably the responsiveness to non-cancer cells in the TME.

• 대한의용생체공학회:의공학회지
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• 제41권1호
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• pp.8-13
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• 2020

To develop cardiovascular simulator capable of implementing pulse waves similar to the human body, accurate information about reflection wave is required. However, the conclusion is still not clear and various discussions are underway. In this study, the pulse wave velocity of the tube used in the experiment was first determined by measuring the pressure waves at two points in a single tube system with the experimental device to implement the pulse wave transmission of blood vessels, and the superposition time and characteristics of the reflection wave were confirmed. After that, an air chamber was set at the reflection site, and the effect of the change of air volume on the reflection wave was investigated. Finally, the effect of the number of branches connected to a single tube on the reflection wave was investigated. The superposition time of the reflection wave can be controlled by the air volume of the air chamber, and the magnitude of the reflection wave is influenced by the number of reflection sites that generate the reflection wave. The results of this study may be of practical assistance to simulator researchers who want to implement pulse wave similar to clinical data. It is expected that the more results similar to clinical are provided, the greater the scope of the simulator's contribution to clinical cardiovascular research.

• Archives of Pharmacal Research
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• 제28권11호
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• pp.1270-1274
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• 2005

The vascular endothelial growth factor (VEGF) plays a key role in angiogenesis, which is a process where new blood vessels develop from the endothelium of a pre-existing vasculature. VEGF exerts its activity by binding to its receptor tyrosine kinase, KDR/Flk-1, which is expressed on the surface of endothelial cells. A methanol extract and organic solvent (n-hexane, ethyl acetate, n-butanol, aqueous) fractions from Rubus coreanus were examined for their inhibitory effects on VEGF binding to the VEGF receptor. The methanol extract from the crude drug were found to significantly inhibit VEGF binding to the VEGF receptor ($IC_{50}$$\thickapprox$27 $\mu$g/mL). Among the fractions examined, the aqueous fraction from the medicinal plant showed potent inhibitory effects against the binding of KDR/Flk-1-Fc to immobilized $VEGF_{165}$ in a dose­dependent manner ($IC_{50}$$\thickapprox$11 $\mu$g/mL). Sanguiin H-6 was isolated as an active principle from the aqueous fraction, and inhibited the binding of KDR/Flk-1-Fc to immobilized $VEGF_{165}$ in a dose­dependent manner ($IC_{50}$$\thickapprox$0.3 $\mu$g/mL). In addition, sanguiin H-6 efficiently blocked the VEGF­induced HUVEC proliferation in a dose-dependent manner ($IC_{50}$$\thickapprox$7.4 $\mu$g/mL) but had no effect on the growth of HT1080 human fibrosarcoma cells. This suggests that sanguiin H-6 might be a potential anti-angiogenic agent.

• 해양환경안전학회지
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• 제19권5호
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• pp.503-510
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• 2013

본 연구에서는 우리나라 해기사들의 행동특성을 파악하고, 이를 통제하기 위한 방안을 제시함으로써 해양안전 문화설계를 용이하게 하고자 하였다. 한국선원 377명을 대상으로 실증분석 결과, 우리나라 선원은 일반적인 한국인의 성격특성 중 "정이 많다"는 것이 가장 뚜렷한 인식을 보였으며, 유교적 관습이 아직도 해상직업 종사자들에게도 영향을 많이 끼치는 것으로 나타났다. 그러나 직책별, 성별, 선종별 집단간에는 행동특성의 차이를 보이지 않았으나 학력별로는 차이가 있는 것으로 나타났다. 한편 한국선박의 안전운항을 위해 동원될 수 있는 수단은 한국선원의 집단 구성에 관계없이 부적 강화물을 제공하는 것보다는 정적 강화물을 제공하는 것이 도움이 될 것으로 판단된다. 선박의 안전관리 수단은 학력수준 외는 집단별 차이가 없는 것으로 나타나, 선원의 학력수준에 맞는 안전관리방안을 도입하는 것이 안전관리의 효율성을 높일 것으로 판단된다.

• Macromolecular Research
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• 제8권4호
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• pp.179-185
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• 2000

Generally vascular grafts with a relatively large inner diameter (> 5 mm) have been successfully employed for replacement in the human body. However, the use of small diameter grafts is limited, because these grafts rapidly occlude due to the thrombosis. The ideal blood-contacting surface of a prosthesis would be an endothelial cell (EC) lining, because the confluent monolayer of healthy ECs that culture natural blood vessels represents the ideal nonthrombogenic surface. For vascular graft application, the stable EC adhesion on surface under How conditions is very important. In this study, the adhesive strength of ECs attached on polymer surfaces coated with collagen type IV (Col IV), fibronectin (Fn), laminin (Ln), and treated with corona was investigated onto polyurethane (PU) films. The EC-attached PU surfaces were mounted on parallel-plate flow chambers in a How system prepared for cell adhesiveness test. Three different shear stresses (100, 150, and 200 dyne/㎠) were applied to the How chambers and each shear stress was maintained for 120 min to investigate the effect of shear stress and surface treatment condition on the EC adhesion strength. It was observed that the EC adhesion strength on the surface-modified PU films was in the order of Ln≡Fn > Col IV > corona 》 control. More than 70% of the adhered cells were remained on surface-modified PU surface after applying the shear stress,200 dyne/㎠ for 2 hrs, whereas the cells were completely detached on the control PU surface within 10 min after applying the same shear stress. It seems that the type of adsorbed proteins and hydrophilicitv onto the PU surfaces play very important roles for cell adhesion strength.

• 비파괴검사학회지
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• 제32권5호
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• pp.484-493
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• 2012

초음파를 사용해 음속도, 감쇠 계수, 밀도, 비선형 B/A 파라미터 등을 측정하여 인체 조직의 특성을 정량적으로 영상화하고자 하는 연구가 1980년대부터 많이 진행되어 왔으나 아직 상용화 단계에는 도달하지 못했다. 하지만 1990년대 초에 시작된 탄성 영상법은 최근 들어 초음파 진단기에 상용화되어 임상에서 B-모드 영상법과 함께 전립선, 유방, 갑상선, 간, 혈관 등을 진단하기 위한 보완적이며 더 정량적인 모드로 사용되고 있는 단계에 진입하였다. 본고에서는 주로 준정적 또는 정적탄성 영상법에 사용되는 여러 가지 알고리듬을 소개하고 특성을 비교하고자 한다. 대부분의 알고리듬은 상호상관함수 또는 자기상관함수 방법에 그 기반을 두고 있으며 전자는 래그를 변화시켜가면서 시간 이동량을 찾지만 후자는 보간 과정 없이 고정된 래그에서의 위상차로부터 시간 이동량을 바로 구해 변위를 추정하는 점이다.