• Journal of Integrative Natural Science
• /
• v.9 no.3
• /
• pp.185-189
• /
• 2016

Urotensin-2 receptor (UTS2R) is the most potent vasoconstrictor and plays a major role in the pathophysiology of various cardiovascular diseases and becomes a potential target for human pharmacotherapy. The crystal structure of Urotension-2 receptor has not yet been resolved. Hence, in the current study homology modelling of UTS2R was done utilizing the crystal structure of human delta opioid receptor as the template. Since the template has low sequence identity, we have incorporated both comparative modelling and threading approach to generate the three dimensional structure. 10 models were generated and validated. The reported models can be used to characterize the critical amino acid residues in the binding site of UTS2R.

• Structural Engineering and Mechanics
• /
• v.83 no.6
• /
• pp.729-744
• /
• 2022

In this paper, an analytical formulation is proposed to predict the vertical vibration response due to the pedestrian walking on a footbridge considering the human-structure interaction, where the footbridge and pedestrian are represented by the Euler beam and linear oscillator model, respectively. The derived coupled equation of motion is a nonlinear fourth-order partial differential equation. An uncoupled solution strategy based on the combined weighted residual and perturbation method) is proposed to reduce the tedious computation, which allows the separate integration between the bridge and pedestrian subsystems. The theoretical study demonstrates that the pedestrian subsystem can be treated as a structural system with added mass, damping, and stiffness. The analysis procedure is then applied to a case study under the conditions of single pedestrian and multi pedestrians, and the results are validated and compared numerically. For convenient vibration design of a footbridge, the simplified peak acceleration formula and the idea of decoupling problem are thus proposed.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 1994.04a
• /
• pp.41-49
• /
• 1994

The most common conventional method of discovering a drug involves a massive screening of a large number of compounds in chemical libraries or in the extracts from natural sources such as plants or microbial broths followed by chemical modification of one or more active compounds to improve their properties as a drug. When the three-dimensional structure of the target molecule for which the drug is searched is known the drug discovery process can be significantly simplified, This is especially true when the three-dimensional structure of a complex between the target and a lead compound is known. In this lecture our experience on the structure-based drug design for human CDK2(cyclin-dependent protein kinase 2) will be discussed with special emphasis on the strength and weakness of this approach of drug discovery. The regulation of the activity of CDK2 plays an important role in the cell proliferation of normal and cancer cells.

• Proceedings of the Korea Society for Simulation Conference
• /
• 2001.05a
• /
• pp.135-140
• /
• 2001

In the last years there have been some severe accidents with passenger vessels. So, International Maritime Organization(IMO) has recognized that computer stimulation of the evacuation may be required for passenger vessels. Human elements is a key issues of escape analysis on shipboard. There are technical requirements to simulate of escape analysis for human elements. Technical requirements include model of ship structure, evacuation algorithm, human behaviour analysis and influence of ship listing/motion. This paper provides the key issues and technologies of simulation for human escape on shipboard.

• Animal cells and systems
• /
• v.7 no.1
• /
• pp.63-68
• /
• 2003

Until recently, interest in human complement receptor type I (CR1) has focused on immune complex processing, which contributed to our understanding of regulatory mechanism of complement activation. However, the promoter structure and transcriptional regulation of human CR1 gene has not been clear. To study the unique regulation of human CR1 gene expression, we assessed promoter activity of the $5^1$-flanking region of human CR1 gene using transient transfection and gel mobility shift assays. In this study we demonstrated that NF-Y binds to the inverted CCAAT element and that the functional interaction with protein(s) which bind to the GC-rich motif may be necessary for optimal transcription of human CR1 gene. We also show that sequence elements which located at-95/58 and +45/+50 are important for optimal transcription of CR1 gene.

• Journal of the Korea Computer Graphics Society
• /
• v.14 no.2
• /
• pp.35-41
• /
• 2008

H-Anim is an international standard that Humanoid Animation Working Group in Web3D Consortium defined the data structure necessary for human animation. Various libraries and tools have been generated according to the structure, but they still have restrictions to represent realistic humanoid motions. This paper presents the method of generating realistic human motion using motion capture data in order to define motion for humanoid animation based on H-Anim standard. In order to implement this, we have defined a data structure capable of receiving motion capture data and implemented a motion browser. The human motion data structure defined in this paper is based on X3D and intended to have compatibility through networks and various browsers.

• Genomics & Informatics
• /
• v.3 no.2
• /
• pp.61-65
• /
• 2005

Comparing 231 genes on chimpanzee chromosome 22 with their orthologous on human chromosome 21, we have found that 15 orthologs have indels within their coding sequences. It was rather surprising that significant number of genes have changed by indel, despite the shorter time since their divergence and led us hypothesize that indels and structural changes may represent one of the major mechanism of proteome evolution in the higher primates. Human T-complex protein 10 like (TCP 10L) is a representative having indel within its coding sequence. Gene structure of human TCP10L compared with chimpanzee TCP10L gene showed 16 base pair difference in genomic DNA. As a result of the indel, frame shift mutation occurs in coding sequence (CDS) and human TCP10L express longer polypeptide of 21 amino acid residues than that of chimpanzee. Our prediction found that the indel may affect to dramatic change of secondary protein structure between human and chimpanzee TCP10L. Especially, the structural changes in the C-terminal region of TCP10L protein may affect on the interacting potential to other proteins rather than DNA binding function of the protein. Through these changes, TCP10L might influence gene expression profiles in liver and testis and subsequently influence the physiological changes required in primate evolution.

• Interdisciplinary Bio Central
• /
• v.2 no.3
• /
• pp.8.1-8.5
• /
• 2010

Introduction: Recently identified human homologues of ALKB protein have shown the activity of DNA damaging drugs, used for cancer therapy. Bioinformatics study of hABH2 and hABH3 had led to the discovery of a novel DNA repair mechanism. Very little is known about structure and function of hABH4, one of the members of this superfamily. Therefore, in present study we are intended to predict its structure and function through various bioinformatics tools. Materials and Methods: Modeling was done with modeler 9v7 to predict the 3D structure of the hABH4 protein. This model was validated with the program Procheck using Ramachandran plot statistics and was submitted to PMDB with ID PM0076284. The 3d2GO server was used to predict the functions. Residues at protein ligand and protein RNA binding sites were predicted with 3dLigandSite and KYG programs respectively. Results and Discussion: 3-D model of hABH4, ALKBH4.B99990003.pdb was predicted and evaluated. Validation result showed that 96.4 % residues lies in favored and additional allowed region of Ramachandran plot. Ligand binding residues prediction showed four Ligand clusters, having 24 ligands in cluster 1. Importantly, conserved pattern of Glu196-X-Pro198- Xn-His254 in the functional domain was detected. DNA and RNA binding sites were also predicted in the model. Conclusion and Prospects: The predicted and validated model of human homologue hABH4 resulted from this study may unveil the mechanism of DNA damage repair in human and accelerate the research on designing of appropriate inhibitors aiding in chemotherapy and cancer related diseases.

• Structural Engineering and Mechanics
• /
• v.43 no.5
• /
• pp.607-621
• /
• 2012

The Expo Culture Centre is one of the permanent buildings at the World Expo 2010 in Shanghai, China. The main structure has an oval shape and consists of 36 radial cantilever steel trusses with different lengths and inner frames made of concrete-filled rectangular steel tube members. Tuned mass dampers are used to reduce the excessive vibrations of the sixth floor that are caused by human-induced resonance. A three-dimensional analytical model of the system is developed, and its main characteristics are established. A series of field tests are performed on the structure, and the test results show that the vertical vibration frequencies of most structural cantilevers are between 2.5 Hz and 3.5 Hz, which falls in the range of human-induced vibration. Twelve pairs of tuned mass dampers weighing 115 tons total were installed in the structure to suppress the vibration response of the system. These mass dampers were tuned to the vertical vibration frequency of the structure, which had the highest possibility of excitation. Test data obtained after the installation of the tuned mass dampers are used to evaluate their effectiveness for the reduction of the vibration acceleration. An analytical model of the structure is calibrated according to the measured dynamic characteristics. An analysis of the modified model is performed and the results show that when people walk normally, the structural vibration was low and the tuned mass dampers have no effect, but when people run at the structural vibration frequency, the tuned mass dampers can reduce the floor vibration acceleration by approximately 15%.

• Journal of Institute of Control, Robotics and Systems
• /
• v.5 no.2
• /
• pp.208-219
• /
• 1999

Springs have been employed in a wide range of mechanical systems. This work deals with the concept of an adaptable spring mechanism which can arbitrarily modulate its spring characteristics. The adaptable spring is desired for enhancing performances of various mechanical systems employing springs. We demonstrate that such adaptable springs can be realized by adapting anthropomorphic musculoskeletal structures of the human upper-extremity, which possesses highly nonlinear kinematic-coupling among redundant muscles existing in its structures. This phenomenon has been explained by several human arm models. Based on the analysis results, we propose multi-degree-of-freedom spring mechanisms resembling the musculoskeletal structure of the human upper-extremity, and verifiy the applicability of these mechanisms through simulation.