• 대한의생명과학회지
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• 제8권4호
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• pp.211-215
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• 2002

The baculovirus/Sf9 cell expression can be employed as a powerful system for producing large amounts of the human erythrocyte glucose transporter, GLUT1 heterologously In order to exploit the system further, it is necessary to develop a convenient method for demonstrating that the transporter expressed in insect cells is biologically active. To achieve this, we have expressed the human CLUT1 in insect cells and photolabelled the expressed protein with [$^3$H] cytochalasin B, a potent inhibitor of the human erythrocyte glucose transporter. Subsequently, the labelled proteins were analysed by SDS-polyacrylamide gel electrophoresis (SDS-PAGE). Membranes labelled with [$^3$H] cytochalasln B in the presence of L-Glucose yielded a single sharp peak of labelling of apparent $M_r$ 45,000 on SDS/polyacrylamide gels. The mobility of this peak corresponded exactly to that of the band detected by anti-glucose transporter antibodies on Western blots of membranes prepared from insect cells infected with recombinant virus. In addition, the sharpness of the radioactive peak provides further evidence for the conclusion that the expressed protein is much less heavily and heterogeneously glycosylated than its erythrocyte counterpart. No peak of labelling was seen with the membranes prepared from non-infected Sf9 cells. Furthermore, the incorporation of label into this peak was completely inhibited by the presence of 500 mM-D-Glucose during tile photolabelling procedure, showing the stereoselectivity of the labelling. These evidences clearly show that human glucose transporter expressed in insect cells exhibits native-like biological activity, and that photolabelling with [$^3$H] cytochalasin B can be a convenient means for analysing the biological activity of the transport protein expressed in insect cells.

• Journal of Animal Science and Technology
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• 제51권2호
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• pp.177-182
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• 2009

산업용 균주인 Pichia pastoris에서 재조합 발현된 당화된 인간유래 MINPP (multiple inositol polyphosphate phosphatase) 효소는 기질 피틴태인(InsP6)에 대한 파이테이즈 효소활성을 나타내었고 탈당화시 그 효소활성이 30% 감소되었다. 그 효소의 기질 $InsP_6$에 대한 최적 pH 활성은 중성범위인 pH 7.4였다. 기질특이성 측면에서 인간 MINPP 효소는 para-nitrophenylphosphate (pNPP), ATP, ribose-1-phosphate (R-1-P)와 같은 유기인산화합물(organic phosphate conjugates)의 분해활성은 매우 낮은 대신, 기질 $InsP_6$를 효과적으로 분해하였고 특히 화학적 자극제인(chemical stimulator)인 1 mM 2-phosphoglycolate (2-PG)의 첨가에 따른 기질 2,3-bisphosphoglycerate (2,3-BPG)에 대한 효소활성이 2-PG를 첨가하지 않을때 보다 1.2배 증가되었지만 기질 $InsP_6$에 대한 효소활성에는 영향을 주지 않았다. 또한 2 mM $Mg^{2+}$ 이온과 100 mM $Cl^-$ 이온의 첨가는 MINPP 효소의 기질 2,3-BPG에 대한 효소활성에 역시 영향을 주지 않았다.

• BMB Reports
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• 제28권5호
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• pp.427-432
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• 1995

Ganglioside GM3 and sialidase activities in human fetal liver have been investigated. Gangliosides were extracted from fetal livers by the Folch-Suzuki method and analyzed by high-performance thin layer chromatography (HPTLC). GM3 increased, but lactosylceramide (LacCer) decreased predominantly over the developmental stages. Sialidase in human fetal liver was mainly localized in the lysosomal fraction and its activity was high in the earlier stages of development. The optimum pH for this enzyme was 4.3~4.4. Sialidase was more active with the ganglioside mixture than with GM3, sialyllactose or fetuin. Fetal liver sialidase was still active (20% activity) in the presence of 25% methanol. These results suggested that the changes of the ganglioside GM3 and sialidase activity may be involved in the regulation of cell growth in human fetal liver during development.

• Animal cells and systems
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• 제2권2호
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• pp.243-248
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• 1998

We have partially sequenced glutaminyl cyclases from several mammalian and one avian species and found that the two cysteine residues of the human glutaminyl cyclase are completely conserved. The mammalian glutaminyl cyclase has been reported to possess reactive thiols (Busby, Jr, et aI., 1987, J BioI Chern 262, 8532-8536). Mutagenesis of these cysteine residues, however, resulted in only a slight decrease in enzyme activity. Likewise, the recombinant human enzyme was completely resistant to attempted chemical modification of the putative reactive thiols. Although the human glutaminyl cyclase did not appear to have reactive thiols, it was sensitive to diethylpyrocarbonate and acetylimidazole, indicating the presence of functionally important histidine and tyrosine residues which could act as acid/base catalysts. Almost identical deuterium solvent isotope effect (1.2 vs 1.3) upon the reaction by the human and papaya enzymes, respectively, provides an evidence both animal and plant glutaminyl cyclases catalyze pyroglutamyl-peptide formation by intramolecular cyclization.

• 한국융합학회논문지
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• 제13권4호
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• pp.203-211
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• 2022

코로나 19 팬데믹으로 인해 온라인 교육 시장은 전세계적으로 성장했으며, 온라인 교육은 미래 교육을 지배할 것이라는 예상이다. 본 연구는 온라인 수업 만족도 및 온라인 수업 사용 행동 의도에 영향을 줄 수 있는 인간적 요인으로 자기효능감, 시스템 요인들로 인지된 사회적 실재감 및 인지된 교수 실재감의 효과를 살펴보고 있다. 본 연구에는 수업에서 추가 점수를 받은 조건으로 236명의 학생들이 온라인 서베이에 참여했다. 연구결과, 사회적 실재감과 교수 실재감이 높은 사람들은 사회적 실재감과 교수 실재감이 낮은 사람들보다 온라인 수업 만족 및 온라인 수업 행동 의도가 높은 것으로 나타났다. 또한, 본 연구는 자기 효능감이 높은 사람들은 자기 효능감이 낮은 사람들보다 더 높은 온라인 수업 만족 및 온라인 수업 행동 의도를 보인다는 결과를 밝혀냈다. 본 연구는 온라인 수업에서 학생들의 만족도 및 온라인 교육 사용 의도를 높이는데 있어 이론적 그리고 실무적 시사점을 제공한다.)

• 셀메드
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• 제1권1호
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• pp.3.1-3.4
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• 2011

Emerging infectious diseases are usually a public concern. The presence of new emerging infectious diseases is a topic to be reported on and discussed about in medicine. Several new emerging infectious diseases have occurred within the present decade. In this specific review, the author briefly reviews the important new emerging human viral infections that first appeared in Asia during the present decade, 2001 - 2010.

• 한국독성학회:학술대회논문집
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• 한국독성학회 2001년도 International Symposium on Dietary and Medicinal Antimutgens and Anticarcinogens
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• pp.154-155
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• 2001

Cyclooxygenase-2 (COX-2) is an inducible enzyme expressed in response to a variety of proinflammatory agents and cytokines. COX-2 expression has been shown to be elevated in several different types of human cancer. The presence of oncogenic ras has been associated with constitutive induction of COX-2 in certain H-ras transformed cells, and COX-2 overexpression confers resistance to apoptosis.(omitted)

• 한국생산제조학회지
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• 제24권1호
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• pp.103-109
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• 2015

This paper introduces a multi-purpose smart fusion sensor. Normally, this type of sensor can contribute to energy savings specifically related to lighting and heating/air conditioning systems by detecting individuals in an office building. If a fire occurs, the sensor can provide information regarding the presence and location of residents in the building to a management center. The system consists of four sensors: a thermopile sensor for detecting heat energy, an ultrasonic sensor for measuring the distance of objects from the sensor, a fire detection sensor, and a passive infrared sensor for detecting temperature change. The system has a wireless communication module to provide the management center with control information for lighting and heating/air conditioning systems. We have also demonstrated the usefulness of the proposed system by applying it to a real environment.

• 생약학회지
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• 제9권4호
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• pp.157-167
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• 1978

Atherosclerosis is associated with the presence of extracellular lipid droplets and large fatty deposits, both of which are to be covered at the surface mainly by zwitterionic phospholipids. The development of atherosclerosis is often associated with the accumulation of calcium. Furthermore, the presence of glycosaminoglycans directly underlying fatty deposits in human aorta has been demonstrated. Also, the possible involvement of the interaction between sulfated polysaccharide and lipoprotein in the development of atherosclerosis has been suggested in view of the presence of both low density lipoproteins and glycosaminoglycans, as well as their complexes, in atherosclerotic aortas. Therefore interactions of sulfated polysaccharides with low density lipoproteins which serve as a vehicle for cholesterol and cholesterol ester and with zwitterionic phospholipids have been studied extensively by a number of workers to provide mechanisms. In this paper, the mechanism of the interaction of sulfated polysaccharides with low density lipoproteins and the mechanism of the interaction between sulfated polysaccharides and zwitterionic phospholipids are reviewed. The possibility of the occurence of these interactions in the body are also considered.

• 기독교교육논총
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• 제70권
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• pp.303-337
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• 2022

본 연구의 목적은 학습자간 대면 접촉이 없는 환경에서 교수-학생간 실재감 인식을 높일 수 있는 상호작용적 요소를 강화한 Ontact PBL을 구현하여 이제 갓 대학을 입학한 대학생들이 1학년 1학기 교양 교과목에서 비대면 수업 환경에서 학습자가 중심의 협력학습을 수행해야 하는 PBL 수업을 통해 교수실재감 및 학습실재감이 향상되었는지 살펴보며, 실재감이 향상된 수업 사례를 제시하고자 하는 연구이다. 이 수업은 2021년 1학기 Ontact PBL을 설계 및 개발하여 운영하였으며, 해당 수업을 수강한 학생들을 대상으로 교수실재감 및 학습실재감을 진단하여 실제로 수업을 통해 교수실재감은 평균 0.95점 유의미하게 향상되어, 학습실재감보다 많이 향상되었고, 이는 학생들의 수업에 대한 성찰 저널에서도 관련 의견이 많아 교수실재감 향상에 효과적임을 알 수 있었다. 더불어 학습실재감도 효과가 있었는데, 학습실재감의 하위요소인 감성적 실재감은 0.7점, 사회적 실재감은 0.5점, 인지적 실재감은 0.6점 가량 유의미하게 향상됨을 확인하였다. 학습실재감 또한 학생들의 수업에 대한 성찰을 확인하여, Ontact PBL이 실재감 향상에 효과가 있다고 판단할 수 있었다. 향후 Ontact PBL의 실재감 향상 요인 연구, Ontact PBL을 통한 학생 역량 향상 연구 등을 후속 연구로 제안한다.