• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2006년도 6th International Meeting on Information Display
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• pp.81-85
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• 2006

The ultra-high definition TV system nicknamed "Super Hi-Vision" is a television system with 4000-scanning-line video and 22.2 multichannel sound. NHK has been developing it with the aim of creating a broadcasting system conveying a strong sensation of presence. We believe that it is important to design the system by taking into account human factors such as human visual system characteristics. This paper describes the psychological and physiological experiments on the visual effects of "Super Hi-Vision".

• Journal of Microbiology and Biotechnology
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• 제30권8호
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• pp.1207-1213
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• 2020

Aflatoxin B1 (AFB1) is a mycotoxin produced by Aspergillus flavus (A. flavus). AFB1 is reported to have high thermal stability and is not decomposed by heat treatment during food processing. Therefore, in this study, knowing that AFB1 is metabolized by cytochrome P450 (CYP), our aim was to develop a method to detoxify A. flavus-contaminated maize, under normal temperature and pressure, using Escherichia coli expressing human CYP3A4. First, the metabolic activity of AFB1 by recombinant human CYP3A4 was evaluated. As a result, we confirmed that recombinant human CYP3A4 metabolizes 98% of AFB1. Next, we found that aflatoxin Q1, a metabolite of AFB1 was no longer mutagenic. Furthermore, we revealed that about 50% of the AFB1 metabolic activity can be maintained for 3 months when E. coli expressing human CYP3A4 is freeze-dried in the presence of trehalose. Finally, we found that 80% of AFB1 in A. flavus-contaminated maize was metabolized by E. coli expressing human CYP3A4 in the presence of surfactant triton X-405 at a final concentration of 10% (v/v). From these results, we conclude that AFB1 in A. flavus-contaminated maize can be detoxified under normal temperature and pressure by using E. coli expressing human CYP3A4.

• Asian Pacific Journal of Cancer Prevention
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• 제16권15호
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• pp.6753-6759
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• 2015

Background: Loss of function of the p53 gene is implicated in defective apoptotic responses of tumors to chemotherapy. Although the pro-apoptotic roles of eugenol and capsaicin have been amply reported, their dependence on p53 for apoptosis induction in gastric cancer cells is not well elucidated. The aim of the study was to elucidate the role of p53 in the induction of apoptosis by eugenol and capsaicin in a human gastric cancer cell line, AGS. Materials and Methods: AGS cells were incubated with or without various concentrations of capsaicin and eugenol for 12 hrs, in the presence and absence of p53 siRNA. Cell cycling, annexin V and expression of apoptosis related proteins Bax, Bcl-2 ratio, p21, cyt c-caspase-9 association, caspase-3 and caspase-8 were studied. Results: In the presence of p53, capsaicin was a more potent pro-apoptotic agent than eugenol. However, silencing of p53 significantly abrogated apoptosis induced by capsaicin but not that by eugenol. Western blot analysis of pro-apoptotic markers revealed that as opposed to capsaicin, eugenol could induce caspase-8 and caspase-3 even in the absence of p53. Conclusions: Unlike capsaicin, eugenol could induce apoptosis both in presence and absence of functional p53. Agents which can induce apoptosis irrespective of the cellular p53 status have immense scope for development as potential anticancer agents.

• Natural Product Sciences
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• 제23권3호
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• pp.175-182
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• 2017

This study evaluated the anti-Helicobacter and anti-inflammatory effects of Sohamhyungtang (SHHT). The minimum inhibitory concentration (MIC) of SHHT against Helicobacter pylori (H. pylori) was determined by the agar dilution method. Expression of the H. pylori cagA gene in the presence of SHHT was determined by quantitative real-time polymerase chain reaction (qRT-PCR). Inhibition of H. pylori urease by SHHT was determined by the phenol-hypochlorite assay. Antiadhesion activity of SHHT was measured by urea-phenol red reagent. Inhibition of nitric oxide (NO) production in AGS cells was measured with Griess reagent. Inducible nitric oxide synthase (iNOS) and IL-8 mRNA expression in AGS cells which were infected with H. pylori was determined by qRT-PCR. IL-8 level was measured by enzyme-linked immunosorbent assay (ELISA). The MIC of SHHT was $100{\mu}g/mL$ and the expression of cagA gene was decreased about 25 folds in the presence of SHHT. H. pylori urease was inhibited 90% by SHHT. SHHT inhibited H. pylori adhesion on AGS cell in a concentration dependent manner. mRNA expression of iNOS and IL-8 and the production of NO and IL-8 were significantly decreased in the presence of SHHT. In conclusion, SHHT showed anti-Helicobacter activity and has potent anti-inflammatory effect on H. pylori-induced inflammation in human gastric epithelial AGS cells.

• 생약학회지
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• 제49권3호
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• pp.255-261
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• 2018

The aim of this study was to evaluate the anti-helicobacter activity and anti-inflammatory activity of Angelica dahurica (AD). The minimum inhibitory concentration(MIC) of AD against Helicobacter pylori(H. pylori), expression of the H. pylori cagA gene in the presence of AD was determined. Inhibition of H. pylori urease by AD, inhibition of nitric oxide (NO) production in AGS cells was measured. IL-8 mRNA expression in AGS cells which were infected with H. pylori and IL-8 level was measured. The MIC of MeOH Ex. of AD was $250{\mu}g/mL$ and the expression of cagA gene was decreased about 88% in the presence of AD. The activity of H. pylori urease was inhibited 70% by AD. mRNA expression of IL-8 and the production of NO and IL-8 were significantly decreased in the presence of AD. In conclusion, AD showed anti-Helicobacter activity and has potent anti-inflammatory effect on H. pylori-induced inflammation in human gastric epithelial AGS cells.

• 감성과학
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• 제10권2호
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• pp.155-168
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• 2007

컴퓨터 에이전트가 우리의 일상에 미치는 영향력이 커지고 에이전트와의 상호작용이 대인 상호작용과 유사하게 변화할수록, 업무적 효율성보다 에이전트와의 상호작용에서 사용자가 겪게 되는 심리적 사회적 경험이 중요해진다. 사용자의 긍정적인 심리적 경험을 유도하기 위해 에이전트와 사용자의 상호작용에서 사회적 실재감(social presence)을 증진시키고자 하는 연구가 이루어져 왔다 이러한 연구들에서는 에이전트의 언어적/비언어적 커뮤니케이션에 대인적 요소를 적용함으로써 사회적 실재감을 증진시키고자 하였다. 본 연구에서는 대인관계에서 매우 중요한 커뮤니케이션 요소인 상호적 자기노출(self-disclosure)이 사용자들의 사회적 실재감 인식과 에이전트 평가, 사용자 경험에 있어 어떠한 효과를 갖는지 살펴보고 개인의 성격변인인 애착유형(attachment styles)은 어떠한 영향을 미치는지 살펴보았다. 또한 사회적 실재감이 에이전트 평가와 사용자 경험에 대한 효과를 매개하는지 알아보았다. 실험 결과, 사용자와 에이전트의 상호적 자기노출 교환은 사회적 실재감을 증진시키고 에이전트에 대한 호의적 평가와 긍정적인 사용자 경험을 유도하였으며, 향후 상호작용 의사를 증진시켰다. 피험자의 애착유형에 따라 사회적 실재감, 에이전트 평가, 사용자 경험, 향후 상호작용 의사가 다르게 나타났다. 종속변인에 대한 자기노출 교환과 애착유형의 효과는 지각된 사회적 실재감에 의해 매개되는 것으로 나타났다. 본 연구의 결과는 에이전트 메시지의 설계에 커뮤니케이션적 측면이 중요하게 고려되어야 함을 시사한다. 또한 에이전트가 사용자들에게 미치는 심리적 효과는 사용자의 성격 특성에 따라 다르게 나타날 수 있다는 것을 시사한다.

• BMB Reports
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• 제39권5호
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• pp.511-515
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• 2006

Alanine aminotransferase (AAT) is mainly synthesized in the liver, and its level in mammalian serum is elevated after acute phase induction. Here we demonstrated that sheep anti-human AAT antibody cross-reacted with amphioxus humoral fluids as well as human serum; and the concentration of AAT in the humoral fluids in amphioxus increased after the acute challenge with lipopolysaccharide, while the level of total proteins remains unchanged. These suggest the presence of the same acute phase response pattern in amphioxus, as observed in some mammalian species. Immunohistochemically, AAT was localized in the hepatic diverticulum, ovary and testis. It appears that the hepatic diverticulum in amphioxus is functionally homologous to the vertebrate liver in respect of AAT synthesis, supporting the hypothesis that the vertebrate liver evolved from the hepatic diverticulum of an amphioxus-like ancestor during early chordate evolution.

• Toxicological Research
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• 제31권2호
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• pp.137-149
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• 2015

Human hepatocytes, with complete hepatic metabolizing enzymes, transporters and cofactors, represent the gold standard for in vitro evaluation of drug metabolism, drug-drug interactions, and hepatotoxicity. Successful cryopreservation of human hepatocytes enables this experimental system to be used routinely. The use of human hepatocytes to evaluate two major adverse drug properties: drug-drug interactions and hepatotoxicity, are summarized in this review. The application of human hepatocytes in metabolism-based drug-drug interaction includes metabolite profiling, pathway identification, P450 inhibition, P450 induction, and uptake and efflux transporter inhibition. The application of human hepatocytes in toxicity evaluation includes in vitro hepatotoxicity and metabolism-based drug toxicity determination. A novel system, the Integrated Discrete Multiple Organ Co-culture (IdMOC) which allows the evaluation of nonhepatic toxicity in the presence of hepatic metabolism, is described.

• 한국환경성돌연변이발암원학회지
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• 제23권3호
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• pp.99-102
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• 2003

IARC classified areca quid as a human carcinogen. Areca quid chewed in Taiwan includes Piper betle inflorescence, which contains high concentrations of safrole (15 mg/fresh weight). Safrole is a documented rodent hepatocarcinogen, and chewing areca quid may contribute to human exposure (420 $\mu$m in saliva). The carcinogenicity of safrole is mediated through 1'-hydroxysafrole formation, followed by sulfonation to an unstable sulfate that reacts to form DNA adducts. Using human liver microsomes and Escherichia coli membranes expressing bicistronic human P450s, CYP2E1 and CYP2C9 were identified as the main P450s involved in the activation of safrole. We have demonstrated the presence of stable safrole-dGMP adducts in human oral tissues following areca quid chewing using $^{32}$ P-postlabeling and HPLC mass spectrometry methods. By studying 88 subjects with a known AQ chewing history and 161 matched controls, we have demonstrated that the presence of safrole-DNA adducts in peripheral blood cells was correlated to AQ chewing, and CYP2E1 seemed to play an important role in the modulation of safrole-DNA adduct formation. We have also shown that safrole can form stable safrole-DNA adducts as well as oxidative damages in rodent liver. However, the stable safrole-DNA adducts may represent a more significant initial lesion as compared to the rapidly repaired safrole-induced 8-hydroxy-2'-deoxyguanosine. This oxidative DNA damage is mediated through the formation of hydoryxchavicol, the major safrole metabolite in human urine. Hydroxychavicol may have gone through two-electron oxidation to the o-quinone; then via one-electron reduction to semiquinone radicals to generate oxidative DNA damage. However, these reactive metabolites can be efficiently conjugated by GSH. These data suggest that safrole may contribute to the initiation of oral carcinogenesis through safrole-DNA adduct and not oxidative DNA damage. In addition, CYP2E1 may modulate this adduct formation.

• 산업융합연구
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• 제21권12호
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• pp.123-129
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• 2023

본 연구는 간호대학생의 인권의식, 공감능력 및 다문화수용성의 정도를 파악하고 상관관계를 파악하기 위해 시도되었다. 자료는 본 연구의 자료수집은 2022년 10월 11일부터 2022년 11월 5일까지 G시 소재 일 대학의 간호대학생 175명을 대상으로 수집되었다. 수집된 자료는 SPSS/WIN 22.0 Program을 이용하여 t-test, pearson's correlation coefficient으로 분석하였다. 본 연구 결과는 다음과 같다. 간호대학생의 인권의식은 간호윤리교육 유무, 외국인이나 이주민 친구 유무에 따라 유의한 차이를 보였다. 본 연구의 결과, 인권인식은 공감능력과 유의한 상관관계가 있었으나, 인권인식과 다문화수용성, 공감능력과 다문화수용성은 유의한 상관관계가 나타나지 않았다. 본 연구 결과는 간호대학생의 전문직으로서 역량 강화에 기여하기 위한 간호교육에 기여하게 될 것이다.