• Title/Summary/Keyword: Human physiology

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Effects of Intrarenal Arterial Infusion of Pro-Atrial Natriuretic Peptides on Renal Function in Unanesthetized Rabbits (가토 신장기능에 미치는 Pro-Atrial Natriuretic Peptide의 영향)

  • Lee, Jeong-Eun;Cho, Kyung-Woo;Kim, Suhn-Hee
    • The Korean Journal of Physiology
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    • v.24 no.1
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    • pp.131-144
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    • 1990
  • It is well known that the atrial natriuretic peptide (ANP) has a prepro-hormone of 151 amino-acids which loses their hydrophobic signal peptide to form 126 amino acid prohormone. The whole prohormone is released and then cleaved by proteases into more than one circulating forms. Recently, Winters et al. (1988a, b) reported that high concentrations of N-terminal fragments of prepro-ANP $(26{\sim}55),\;(56{\sim}92)\;and\;(104{\sim}123)$ were detected in human plasma. However, their physiological roles have not been established. The present study was conducted to determine whether the N-terminal fragments of pro-ANP have any effect on the renal function and to compare the effect with those of G-terminal fragments of pro-ANP The results indicate that intrarenal arterial infusions of prepro-ANP $(26{\sim}41),\;(26{\sim}55),\;(56{\sim}92)\;and\;(104{\sim}123)$ induced no significant changes in renal function. Whereas ${\alpha}-human$ ANP $(prepro-ANP,\;124{\sim}151)$ and pro-ANP caused a significant increase in urine volume, renal plasma flow, glomerular filtration rate, urinary excretions of sodium, chloride and potassium, and fractional excretion of sodium. These results suggest that the N-terminal fragments of pro-ANP are ineffective, while the C-terminal fragments retain the natriuretic and diuretic activities.

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Study on the History of Diagnostic Significanes of the Dream (몽진(夢診)의 역사에 관한 연구)

  • Kang, Dong-Yun;Kang, Jung-Soo
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.21 no.4
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    • pp.842-848
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    • 2007
  • We spend a quarter of every night on the dream, it means for us to spend about six years having a dream. But, any portion of the dream are remained the mystery in these days and it goes without saying that these mystery have not been solved from old times. So. the people have not known about the essences of dream for a long time although they have experienced in dreaming in the early days of human history. But, It is necessary to consider the dream to modality of disease diagnosis because many evidences that the dream represents the physiologic and pathologic changes in the human have been proved in recent days. So, we are going to inquire into the things being scattered over many texts of the Oriental Medicine, including the Internal Classics(內經). about the dream and to look the diagnostic significances of the dream with some examples of the modern psychiatric accomplishments.

Carbon monoxide activates large-conductance calcium-activated potassium channels of human cardiac fibroblasts through various mechanisms

  • Bae, Hyemi;Kim, Taeho;Lim, Inja
    • The Korean Journal of Physiology and Pharmacology
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    • v.25 no.3
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    • pp.227-237
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    • 2021
  • Carbon monoxide (CO) is a cardioprotectant and potential cardiovascular therapeutic agent. Human cardiac fibroblasts (HCFs) are important determinants of myocardial structure and function. Large-conductance Ca2+-activated K+ (BK) channel is a potential therapeutic target for cardiovascular disease. We investigated whether CO modulates BK channels and the signaling pathways in HCFs using whole-cell mode patch-clamp recordings. CO-releasing molecules (CORMs; CORM-2 and CORM-3) significantly increased the amplitudes of BK currents (IBK). The CO-induced stimulating effects on IBK were blocked by pre-treatment with specific nitric oxide synthase (NOS) blockers (L-NG-monomethyl arginine citrate and L-NG-nitroarginine methyl ester). 8-bromo-cyclic GMP increased IBK. KT5823 (inhibits PKG) or ODQ (inhibits soluble guanylate cyclase) blocked the CO-stimulating effect on IBK. Moreover, 8-bromo-cyclic AMP also increased IBK, and pre-treatment with KT5720 (inhibits PKA) or SQ22536 (inhibits adenylate cyclase) blocked the CO effect. Pre-treatment with N-ethylmaleimide (a thiol-alkylating reagent) also blocked the CO effect on IBK, and DL-dithiothreitol (a reducing agent) reversed the CO effect. These data suggest that CO activates IBK through NO via the NOS and through the PKG, PKA, and S-nitrosylation pathways.

Traditional Korean Medicine Research Using Methods in Complexity Science: Current Status and Prospect (복잡계 과학 방법론을 활용한 한의학 연구: 현황과 전망)

  • Jang, Dongyeop;Cho, Na-Hyun;Lee, Ki-Eun;Kwon, Young-Kyu;Kim, Chang-Eop
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.35 no.5
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    • pp.151-161
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    • 2021
  • Traditional Korean medicine (TKM) takes a holistic view that emphasizes the balance between the elements constituting the human body or between the human body and the external environment. To investigate the holistic properties of TKM, here we propose to apply the methodology of complexity science to the TKM research. Complexity science is a discipline for studying complex systems with interactions between components that raise the behaviour as a whole which can be more than the sum of their parts. We first provide an introduction to the complexity science and its research methods, particularly focusing on network science and data science approaches. Next, we briefly present the current status of TKM research employing these methods. Finally, we provide suggestions for future research elucidating the underlying mechanism of TKM, both in terms of biomedicine and humanities.

Apoptin gene delivery by a PAMAM dendrimer modified with a nuclear localization signal peptide as a gene carrier for brain cancer therapy

  • Bae, Yoonhee;Lee, Jell;Kho, Changwon;Choi, Joon Sig;Han, Jin
    • The Korean Journal of Physiology and Pharmacology
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    • v.25 no.5
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    • pp.467-478
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    • 2021
  • In this study, we aimed to synthesize PAMAMG3 derivatives (PAMAMG3-KRRR and PAMAMG3-HKRRR), using KRRR peptides as a nuclear localization signal and introduced histidine residues into the KRRR-grafted PAMAMG3 for delivering a therapeutic, carcinoma cell-selective apoptosis gene, apoptin into human primary glioma (GBL-14) cells and human dermal fibroblasts. We examined their cytotoxicity and gene expression using luciferase activity and enhanced green fluorescent protein PAMAMG3 derivatives in both cell lines. We treated cells with PAMAMG3 derivative/apoptin complexes and investigated their intracellular distribution using confocal microscopy. The PAMAMG3-KRRR and PAMAMG3-HKRRR dendrimers were found to escape from endolysosomes into the cytosol. The JC-1 assay, glutathione levels, and Annexin V staining results showed that apoptin triggered cell death in GBL-14 cells. Overall, these findings indicated that the PAMAMG3-HKRRR/apoptin complex is a potential candidate for an effective nonviral gene delivery system for brain tumor therapy in vitro.

Yoga Training Improves Metabolic Parameters in Obese Boys

  • Seo, Dae-Yun;Lee, Sung-Ryul;Figueroa, Arturo;Kim, Hyoung-Kyu;Baek, Yeong-Ho;Kwak, Yi-Sub;Kim, Na-Ri;Choi, Tae-Hoon;Rhee, Byoung-Doo;Ko, Kyung-Soo;Park, Byung-Joo;Park, Song-Young;Han, Jin
    • The Korean Journal of Physiology and Pharmacology
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    • v.16 no.3
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    • pp.175-180
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    • 2012
  • Yoga has been known to have stimulatory or inhibitory effects on the metabolic parameters and to be uncomplicated therapy for obesity. The purpose of the present study was to test the effect of an 8-week of yoga-asana training on body composition, lipid profile, and insulin resistance (IR) in obese adolescent boys. Twenty volunteers with body mass index (BMI) greater than the 95th percentile were randomly assigned to yoga (age $14.7{\pm}0.5$ years, n=10) and control groups (age $14.6{\pm}1.0$ years, n=10). The yoga group performed exercises three times per week at 40~60% of heart-rate reserve (HRR) for 8 weeks. IR was determined with the homeostasis model assessment of insulin resistance (HOMA-IR). After yoga training, body weight, BMI, fat mass (FM), and body fat % (BF %) were significantly decreased, and fat-free mass and basal metabolic rate were significantly increased than baseline values. FM and BF % were significantly improved in the yoga group compared with the control group (p<0.05). Total cholesterol (TC) was significantly decreased in the yoga group (p<0.01). HDL-cholesterol was decreased in both groups (p<0.05). No significant changes were observed between or within groups for triglycerides, LDL-cholesterol, glucose, insulin, and HOMA-IR. Our findings show that an 8-week of yoga training improves body composition and TC levels in obese adolescent boys, suggesting that yoga training may be effective in controlling some metabolic syndrome factors in obese adolescent boys.

Color therapy and application of color to oriental medical science (색채치료(色彩治療)와 한의학(漢醫學)에서의 색(色)의 적용(適用))

  • Park, Seung Lim;Kang, Jung Soo
    • Journal of Haehwa Medicine
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    • v.12 no.1
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    • pp.79-102
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    • 2003
  • It has been believed that the human body can be effected by color, sound, smell, and taste - each of them is based on the original character. Out of these elements, the color can be a mediation that absorbs energy into human body and adapts it to the creatures whose life are definitely based on the solar energy. This thesis makes a study of the possibility of applying the color to the oriental medical science by researching the color psychology and therapy which are studied in the west medical science, the recognition and application of color developed from orientalism, and the opinion of color in the oriental medical science. Color therapy is one of the psychological treatment techniques that are able to recover and maintain the health with the rays of the sun rays and the color. The light and the color have its energy that may relax, harmonize, encourage, or excite a human being. This is because the wavelength and the vibration of each color will take effects to human body. The core energy of absorbing and distributing the color vibration is made in the 7 "chakras" in the body, which are the pith and marrow of bio-energy directly connected with the center of the nervous system. There are several methods in the color therapy - the treatment of solar ray, the color-bath, the water-therapy using color energy, the inducement of the solar energy into the body, the acupuncture, the therapy of crystal and jewel, and the self-suggestion dependent upon the color. The color therapy can help us to keep our balance by changing the emotion into the positive energy that will cure the disease with color. As a result, this method not only must be useful to cure physical disease, or develop into good health but also will be used in conforming itself to the mental disease. The color therapy mainly uses the eight colors, which are made by mixing Red, Yellow and Blue basic colors in the field. They are never used in the treatment, but they will go along with complementary colors. This rule is closely connected with the theory of yin and yang which lies at the root of oriental philosophy, and with the treatment principle of oriental medical science whose field is focused on the balancing the body mentally as well as physically. In the East, it is the Obangsaek - the color of blue, red, yellow, white, and black in the theory of yin and yang and the five elements that have been used in helping people having trouble with their health for a long time. In the view of the oriental medical science, these five colors attached the theory of five-elements have been very useful to the physiology, the pathology, or the diagnosis, and been applied to the medical treatment, combining themselves with a five-taste in medicinal herbs. Since the study of color and human physiology has been made in some different interest and fields these days, it goes to prove that the different function of color we couldn't have got before becomes very useful to the medical science. The color must be worth researching the diversity for applying itself to the oriental medical science.

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