• Title/Summary/Keyword: Human embryonic stem cells

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Directed Differentiation of Pancreatic Islets from Human Embryonic Stem Cells and Cell Therapy of Diabetes Mellitus (인간배아줄기세포를 이용한 췌장세포의 유도 분화 및 당뇨병의 세포치료)

  • Kim, Suel-Kee;Shim, Joong-Hyun;Woo, Dong-Hun;Kim, Jong-Hoon
    • Development and Reproduction
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    • v.11 no.2
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    • pp.67-77
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    • 2007
  • Replacement of insulin-producing cells represents an almost ideal treatment for patients with diabetes mellitus type 1. Transplantation of pancreatic islets of Langerhans is limited by the lack of donor organs. Therefore, generation of insulin-producing cells from human embryonic stem cells represents an attractive alternative. The present review summarizes the current knowledge on the differentiation of insulin-producing cells from human embryonic stem cells and their application to the cell therapy for treating diabetes mellitus.

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Monitoring the Differentiation and Migration Patterns of Neural Cells Derived from Human Embryonic Stem Cells Using a Microfluidic Culture System

  • Lee, Nayeon;Park, Jae Woo;Kim, Hyung Joon;Yeon, Ju Hun;Kwon, Jihye;Ko, Jung Jae;Oh, Seung-Hun;Kim, Hyun Sook;Kim, Aeri;Han, Baek Soo;Lee, Sang Chul;Jeon, Noo Li;Song, Jihwan
    • Molecules and Cells
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    • v.37 no.6
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    • pp.497-502
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    • 2014
  • Microfluidics can provide unique experimental tools to visualize the development of neural structures within a microscale device, which is followed by guidance of neurite growth in the axonal isolation compartment. We utilized microfluidics technology to monitor the differentiation and migration of neural cells derived from human embryonic stem cells (hESCs). We co-cultured hESCs with PA6 stromal cells, and isolated neural rosette-like structures, which subsequently formed neurospheres in suspension culture. Tuj1-positive neural cells, but not nestin-positive neural precursor cells (NPCs), were able to enter the microfluidics grooves (microchannels), suggesting that neural cell-migratory capacity was dependent upon neuronal differentiation stage. We also showed that bundles of axons formed and extended into the microchannels. Taken together, these results demonstrated that microfluidics technology can provide useful tools to study neurite outgrowth and axon guidance of neural cells, which are derived from human embryonic stem cells.

Forced Expression of HoxB4 Enhances Hematopoietic Differentiation by Human Embryonic Stem Cells

  • Lee, Gab Sang;Kim, Byung Soo;Sheih, Jae-hung;Moore, Malcolm AS
    • Molecules and Cells
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    • v.25 no.4
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    • pp.487-493
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    • 2008
  • HoxB4 has been shown to enhance hematopoietic engraftment by hematopoietic stem cells (HSC) from differentiating mouse embryonic stem cell (mESC) cultures. Here we examined the effect of ectopic expression of HoxB4 in differentiated human embryonic stem cells (hESCs). Stable HoxB4-expressing hESCs were established by lentiviral transduction, and the forced expression of HoxB4 did not affect stem cell features. HoxB4-expressing hESC-derived CD34+ cells generated higher numbers of erythroid and blast-like colonies than controls. The number of CD34+ cells increased but CD45+ and KDR+ cell numbers were not significantly affected. When the hESC derived CD34+ cells were transplanted into $NOD/SCID{\beta}2m-/-$ mice, the ectopic expression of HoxB4 did not alter their repopulating capacity. Our findings show that overexpression of HoxB4 in differentiating hESCs increases hematopoietic colony formation and hematopoietic cell formation in vitro, but does not affect in vivo repopulation in adult mice hosts.