• Korean Journal of Human Ecology
• /
• v.15 no.1
• /
• pp.107-128
• /
• 2006

The purposes of this study are to explore the consumption expenditure and consumption-cultural meaning in the process of marriage and to provide consumer education programs for premarital couples. For the purpose, this study uses a qualitative analysis including an in-depth interview and a participant observation. Major findings are as follows: Consumers preparing for their marriage experienced quite a different consumption context, which should be said an exceptional practice from the economic point of view. As the agency businesses for marriage becomes one of the fast growing industries, the marriage of Korea is now under the rule of popular consumption culture. Thus, the exceptional consumption abuse during a marriage process brings about the imbalance in consumers' daily life after wedding ceremony. In this point of view, we can see the importance of consumer education programs for the marriage process. The latter half of this study is devoted to propose the desirable model of the consumer education program for premarital couples.

• Clinical and Experimental Reproductive Medicine
• /
• v.25 no.3
• /
• pp.299-304
• /
• 1998

Despite the rapid development of assisted reproductive technologies (ART) in recent years, implantation rates after replacement of embryos into the uterine cavity remains low. Several techniques such as culture conditions based on formulations of human tubal fluid and various ART techniques as GIFT, ZIFT, TET have been adopted in recent years to improve embryo viability in vitro and implantation rates. Also, coculture of human IVF-derived embryos have been used in an effort to increase the number of viable embryos following IVF and to improve synchrony between the developing embryo and the uterine environment. The aim of this study was to evaluate whether the use of co culture with autologous cumulus cells has a significant beneficial effect on the development of embryos in vitro and its relation to the pregnancy rates in 120 patients with previous failed IVF-ET from September, 1995 to January 1998. We obtained the results from which significant improvement in the quality of viable embryos were observed using a coculture system with autologous cumulus cells, but pregnancy rates in this group of patients did not differ from the rate in the standard IVF group during the same period. Our study shows that a simplified short-term coculture system with autologous cumulus cells may help rescue moderate quality embryos to cleave regularly.

• Journal of Microbiology and Biotechnology
• /
• v.17 no.7
• /
• pp.1113-1119
• /
• 2007

Human bone marrow-derived mesenchymal stem cells (hBMMSCs) must differentiate into osteogenic cells to allow for successful bone regeneration. In this study, we investigated the effects of different combinations of three soluble osteogenic differentiation-inducing factors [L-ascorbic acid (AC), ${\beta}$-glycerophosphate (${\beta}G$), and bone morphogenic protein-2 (BMP-2)] and the presence of a hydroxyapatite (HA) substrate on hBMMSC osteogenic differentiation in vitro. hBMMSCs were cultured in medium containing various combinations of the soluble factors on culture plates with or without HA coating. After 7 days of culture, alkaline phosphatase (ALP) activity, calcium deposition, and osteoprotegerin (OPG) and osteopontin (OPN) expression were measured. The effects of individual and combined factors were evaluated using a factorial analysis method. BMP-2 predominantly affected expression of early markers of osteogenic differentiation (ALP and OPG). HA had the highest positive effect on OPN expression and calcium deposition. The interaction between AC, ${\beta}G$, and HA had the second highest positive effect on ALP activity.

• International Journal of Human Ecology
• /
• v.8 no.1
• /
• pp.95-107
• /
• 2007

The present study examined generational differences in ethnicity maintenance among Korean-Chinese to understand the impact of recent social change on a Korean-Chinese ethnic community in China. A total of 1355 Korean-Chinese (557 parents and 798 children), who live in Shenyang, China, participated in this study. The subjects were asked about their language use during daily conversations and cultural activities. They were also asked about their ethnic identity and perceptions of social distance from Chinese people. The results reveal that the Korean-Chinese parent generation is more likely to maintain its ethnic language, while the child generation is more likely to maintain its ethnic culture. Second, more parents than children considered themselves as 'Korean-Chinese' rather than 'Chinese'. Third, members of the child generation show less social distance from Chinese people than do the parent generation. These results show a strong tendency towards ethnicity maintenance among Korean Chinese as well as recent changes in the community. This study argues for the importance of school education and school environment in maintaining the ethnic language and culture of Korean-Chinese children.

• Journal of information and communication convergence engineering
• /
• v.8 no.5
• /
• pp.595-601
• /
• 2010

The strategy through visual sense is one of the ways that subject builds the outside world and communicates. The visual sense seems higher level of dependence than the other senses and contributes to intercommunication. For this reason, the desire of image dates back to primitive art and visual image(visual media and visual culture) has dialectically developed in the history of mankind. Visual subject, based on perspective of Renaissance, was moved from God to human beings. Andre Bazin's 'la genese automatique' through technical art has epochally changed the paradigm of visual art and visual culture. From primitive art to photo and film, the image, based on visual sense, has reflected human wish, appealed visual desire and led to evolution of image. In the late 20 century, without dialectical evolution of technology and culture, rapidly progressive digital image has changed social and cultural implication over rational strategy of production and distribution and it strengthened authority of image through visual approach of endless desire. The goal of this study is to analyze the value change of digital image developing a new Renaissance through production, reading, communication, and implication of evolution due to digitalized image, which has evolved as object and tool of desire.

• Asian-Australasian Journal of Animal Sciences
• /
• v.15 no.11
• /
• pp.1559-1563
• /
• 2002

The ovarian follicular fluid was found to contain steroidogenesis stimulatory protein similar to albumin from human and buffalo. Therefore, the albumins from various species, commercial and purified, were studied for their steroidogenic effect on progesterone secretion by granulosa cells from buffalo ovaries, during culture. A dose of $20{\mu}g$ of bovine serum albumin was optimum to exhibit maximum progesterone secretion on day 6 of culture, in medium ($350{\mu}l$) containing $10^5$ cells. Among commercial albumins, chicken albumin showed highest effect on progesterone secretion, which was followed by albumins from goat, bovine, human, sheep and rat, respectively at day 6 of culture. The albumins were also purified from blood serum of buffalo, goat and rat using salt fractionation, ion-exchange chromatography, gel filtration and SDS-PAGE. The highest stimulatory effect on progesterone secretion was shown by albumin purified from buffalo blood serum and lowest by that from rat blood. Comparatively the buffalo and goat albumins were more biologically active than commercial albumins. The presence of some active molecules conjugated with freshly purified albumins may be responsible for better stimulatory effect.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.5 no.3
• /
• pp.207-210
• /
• 2000

When endothelial cells isolated isolated from human umbilical venis were cultred for 6dary using 96-well microplates, the final cell density in each was fiund not to be the same although the medium composition of each well was exactly the same. Cell growth in the wells located at the periphery of a microplate was low, while that in the central area of the plate was high. A possible cause for different rate of growth was proposed as the uneven concentration of oxygen in the culture medium of each well.

• Journal of the Korean Society of Food Culture
• /
• v.17 no.5
• /
• pp.543-550
• /
• 2002

The purpose of this study is to provide basic materials for food service consumption culture by proving the problem with food service purchase and its improvements. For this purpose, it was attempted to grasp university students' food service purchase behavior and level of satisfaction with it and to investigate the factors influencing their level of satisfaction with food service purchase. The questionnaire research was conducted for university students living in Seoul and Chungcheong provinces. 453 questionnaires obtained from them were used for final analysis. As a results, the following finding were obtained: 1. In case of university students, their level of consumer satisfaction with food service purchase was shown to be the score of 37.99(63.06/on the basis of 100 points). They showed the highest level of satisfaction with quality, followed by facility and atmosphere, service, price and the like. 2. The factor having the greatest influence on university students' level of satisfaction with food service purchase was shown to be gender(female), followed by food service place(Korean food restaurant, Chinese food restaurant, Western food restaurant, flour-based meals restaurant), consumer attitude, average monthly food service cost.

• Microbiology and Biotechnology Letters
• /
• v.22 no.4
• /
• pp.360-367
• /
• 1994

The effect of various nutrients for the production of the anti-complementary polysaccha- ride by Flammulina velutipes, was examined and the partial purification of the polysaccharide was carried out. The culture conditions and medium compositions considerably influenced the anti-complementary activity of the polysaccharides. When a culture was carried out at 23$\circ$C and pH 5.5 for 6 days in the synthetic medium supplemented with galactose and NaNO$_{3}$, the production of the anti-complementary polysaccharide was maximized. The crude polysaccharide, FV-1 was isolated from the culture broth and partially purified into two fractions, FV-1-II and FV-1-III by gel filtration using Sephadex G-100. FV-1-II was mainly consisted of xylose, mannose, galactose and glucose, and rhamnose, mannose and galactose, for FV-1-III. Also, the anti-complementary activity of FV-1-III was reduced partially in the absence of the Ca ion. When crossed immunoelect- rophoresis using anti-human C3 serum was carried out after incubation of normal human serum with the FV-1-III in the Ca ion free condition, a cleavage of C3 precipitin line was observed. These results indicate that the mode of complement activation by polysaccharide from Flammulina velutipes is via not only the classical pathway but also the alternative pathway.

• Korean Journal of Human Ecology
• /
• v.5 no.2
• /
• pp.75-91
• /
• 2002

The purpose of this research is to investigate and analysis the style of Audrey Hepburn(1929-1993), the most important style icon in the 1950s and '60s from the point of the style of dress in her films - Roman Holiday (1953), Sabrina (1954), Funny Face (1957) and Breakfast at Tiffany's (1966) and fashion activities, furthermore, to know the influence on the contemporary fashion. After 1960s, her style has been recreated by so many designers. In the 1980s, times influenced by post-modernism, there was also the effort to reconstruct the style in the 1950s again. At that time, some Hepburn styles-a reflective of the flamboyant and exaggerating trend in her ages-were appeared by a few designers in the 1980s. Over the 1990s' retro culture, there were a lot of efforts to reemerge the styles in the 1950 and '60s, they led to full-scale reconstruction of Hepburn style - a feeling of the elegance and intelligence harmonizing with the minimal and formal style. By the retro culture adding with the mainstream fashion trend of simpleness and comfort in the year 2000, Hepburn's casual style has been reconstructed to meet the modem need in the 21s1 century. Through these considerations, we could notice that Hepburn style has been recreating from various fashion fields harmonizing with the popular trend of the age.