Purpose: This study measured and analyzed the PTO (power take off) torque of a transplanter according to the planting conditions during field operation. Methods: A torque measurement system was constructed with torque sensors to measure the torque of a PTO shaft, a measurement device to acquire sensor signals, and a power controller to provide power for a laptop computer. The field operation was conducted at four planting distances (26, 35, 43, and 80 cm) and two planting depths using the transplanter on a field with similar soil conditions. One-way ANOVA with planting distance and Duncan's multiple range test at a significance level of 0.05 were used to analyze the PTO torque. The torque ratio was calculated based on the minimum torque using the average PTO torque measured under each planting condition. Results: The average torques on the PTO shaft for planting distances of 26, 35, 43, and 80 cm at a low planting depth were 11.05, 9.07, 7.04, and 3.75 Nm, respectively; the same for planting distances of 26, 35, 43, and 80 cm at a middle planting depth were 12.20, 9.86, 7.94, and 4.32 Nm, respectively. When the planting distance decreased by 43, 35, and 26 cm, the torque ratio at a low planting depth increased by 88, 142, and 195%, respectively. When the planting distance decreased by 43, 35, and 26 cm, the torque ratio at the middle planting depth increased by 84, 128, and 182%, respectively. Conclusions: PTO torque fluctuated by planting distance and depth. Moreover, the PTO torque increased for short planting distances. Therefore, farmers should determine the planting conditions of the transplanter by considering the load and durability of the machine. The results of this study provide useful information pertaining to the optimum PTO design of the transplanter considering the field load.