• KSBB Journal
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• 제27권3호
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• pp.186-194
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• 2012

Various human host cell lines, which are more effective than the other original human cell lines, have been developed and used. Highly efficient human cell line can be obtained from the fusion between human embryonic kidney 293 (HEK293) and human Burkitt's lymphoma cells (Namalwa). Fused cell line has the advantages of both cell lines such as the high transfection efficacy of HEK293 cells and the constitutive expression of Epstein-Barr virus (EBV) genome which is related with high expression of target protein and anti-apoptotic growth of Namalwa cells. In this study, characterization of two original cell lines was performed by using design of experiment (DOE) considering cell maintenance, media development, optimization of culture condition, and scale-up. The formation of aggregates was apparent with high glutamine concentration at more than 6 mM. Supplementation of hydrolysates showed positive effects on the growth performances of HEK293 cells. On the contrary, Namalwa cells showed negative results. It was confirmed that Namalwa cells were more sensitive to lower temperature at $35^{\circ}C$ and hyperosmotic condition over 260 mOsm/kg. In addition, both cell lines showed limited growth in 3-L bioreactor due to shear stress.

• 지능정보연구
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• 제27권1호
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• pp.129-149
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• 2021

최근 인간과 사회적으로 상호작용할 수 있는 소셜 로봇(Social Robot)에 대한 관심이 커지고 있다. ICT 기술 발전에 힘입어 소셜 로봇이 개인에게 맞춤형 서비스와 정서적 교감을 제공하기 쉬워졌으며, 현대의 사회문제들과 이로 인한 개인의 삶의 질 저하를 해소하기 위한 수단으로 소셜 로봇의 역할이 주목받고 있다. 소셜 로봇에 대한 관심에 힘입어 소셜 로봇 보급 또한 크게 늘고 있다. 많은 기업이 다양한 목표시장을 겨냥하기 위한 로봇 제품들을 시장에 선보이고 있으나, 현재까지 시장을 선도하는 명확한 흐름은 부재하다. 이에 따라 소셜 로봇의 디자인을 통해 로봇을 차별화하고자 하는 시도가 늘고 있다. 특히 의인화는 소셜 로봇 디자인에서 중요하게 연구되고 있으며, 소셜 로봇을 의인화하여 긍정적인 효과를 발현하려는 접근이 많이 시도되었다. 그러나 소셜 로봇에 대한 의인화가 형성되는 메커니즘을 체계적으로 설명하는 연구는 부족하다. 의인화에 대한 모호한 이해는 소셜 로봇의 의인화를 형성하기 위한 디자인 최적점의 도출을 어렵게 하고 있다. 본 연구는 소셜 로봇의 의인화가 형성되는 메커니즘을 검증하는 것을 목적으로 한다. 본 연구는 3×2 Mixed Design의 실험 연구를 통해 소셜 로봇의 인간 유사성(Human-likeness)과 개인의 해석수준(Construal Level)이 의인화 형성에 미치는 영향을 확인하였다. 의인화가 형성되는 메커니즘에 대한 6개의 연구 가설을 제시하고, 206명 표본의 데이터를 분석하여 가설을 검증하였다. 분석 결과 소셜 로봇의 인간 유사성 수준에 따라 로봇 의인화 수준이 높아지며, 소비자 해석수준에 따라 인간 유사성이 의인화에 미치는 영향이 다르게 나타남을 확인하였다. 본 연구는 소셜 로봇의 디자인 속성인 인간 유사성과 개인의 사고방식인 해석수준을 함께 고려하여 의인화가 형성되는 메커니즘을 설명하였다는 점에서 시사점이 있다. 본 연구의 결과를 소셜 로봇 의인화 형성을 위한 디자인 최적화의 기준으로 활용할 수 있을 것으로 기대한다.

• 동의생리병리학회지
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• 제19권4호
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• pp.1032-1038
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• 2005

Using the 2-channel DROS SQUID (Korea Research Institute of Standards of Science, 1999), the present study was carried out to record changes elicited in the auditory cortex by acupuncture stimulus (right GB43, Xiaxi). Needle-retention and manual needle-twitching stimulation of GB43 and SP1 were done for acquiring the brain activities changed by acupuncture. Acupoint GB43 is known to be effective for the treatment of ear-related disease, such as deafness and tinnitus, and to be suspected to be related to the auditory cortex. Auditory evoked magnetic fields were recorded from the left hemisphere of five or four subjects, in response to contralateral ear stimulation by irregularly spaced 170msec long 1kHz tone busts (Korea Research Institute of Standards of Science). The result as follows The latency and amplitude of SQUID MEG responses at the human auditory cortex changed by needle-retention condition on GB43 were 7.2msec and 1.617, respectively, which were slower and larger than those of no-acupuncture condition. The amplitude of SQUID MEG responses at the human auditory cortex changed by needle-twitching condition on GB43 was 13.517, which was larger than that of no-acupuncture condition. The change in SP1 following GB43 needle-twitching condition were not observed in latency. The amplitude changed by needle-twitching condition on SP1 was 12.2fT, which was not significant. These results suggested that auditory cortex can be affected by acupuncture stimulus, though not specific or significant because of small number of subjects.

• 한국의류산업학회지
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• 제16권1호
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• pp.167-174
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• 2014

The physical properties of textile materials and thermal physiological responses of the human subjects were evaluated with 4 different types of the army combat uniforms including US, German, Korean and general uniforms for this study. 8 male adults were used as the human subjects and the tests were done in the environmental chamber that was $25{\pm}0.1^{\circ}C$ of temperature, $65{\pm}5%$ of relative humidity and below 0.3 m/sec of air velocity. The test protocol consisted the rest period for 20 min., the exercise period for 20 min., the rest period for 20 min., the exercise period under wind condition for 20 min., and the recovery period for 20 min. The human subjects walked with 4.5 km/hr for 10 min., ran 7.5 km/hr for 10 min. during the first exercise period and walked and ran with the same speeds under 3.5 m/sec of the air velocity that simulated outdoor condition during the second exercise period. The test results of the study were as follows; The wind condition affected the skin and microclimate temperature of the human subjects lower compared to without wind condition, but had insignificant effect on the humidity control. The low air permeability of Korean uniform caused blocking the elimination of the humidity from the body and the regulation of body temperature. However, Korean uniforms could be the excellent one with the designs considering the ventilation of the uniforms and the textile fabrics with better air permeability.

• 감성과학
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• 제6권2호
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• pp.9-16
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• 2003

본 연구는 인간의 물리적인 요소(머리너비, 가슴너비, 팔길이 등)에 따른 반응속도의 변화에 대해 다룬다. 본 연구에 따르면, 머리 앞뒤 너비나 팔길이 등의 요소들은 반응속도에 영향을 미치지 않았으나, 너비에 해당하는 요소들은 인간의 반응속도에 영향을 미치는 것으로 평가되었다. 본 연구의 결과는 특수상황에 보다 우수한 작업자를 선정하거나, 운동선수를 테스트 없이 우선적으로 선정하는 데 사용될 수 있다. 또한, 본 연구의 결과는 인간정보전달 경로상의 노이즈라고 명명된 채널 노이즈와 시간과의 관계를 규명하기 위한 채널 노이즈 타임분석을 위해 사용될 수 있다.

• 센서학회지
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• 제29권3호
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• pp.194-200
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• 2020

Human detection is an important aspect in many video-based sensing and monitoring systems. Studies have been actively conducted for the automatic detection of humans in camera images, and various methods have been proposed. However, there are still problems in terms of performance and computational cost. In this paper, we describe a method for efficient human detection in the field of view of a camera, which may be static or moving, through multiple processing steps. A detection line is designated at the position where a human appears first in a sensing area, and only the one-dimensional gray pixel values of the line are monitored. If any noticeable change occurs in the detection line, corner detection and optical flow computation are performed in the vicinity of the detection line to confirm the change. When significant changes are observed in the corner numbers and optical flow vectors, the final determination of human presence in the monitoring area is performed using the Histograms of Oriented Gradients method and a Support Vector Machine. The proposed method requires processing only specific small areas of two consecutive gray images. Furthermore, this method enables operation not only in a static condition with a fixed camera, but also in a dynamic condition such as an operation using a camera attached to a moving vehicle.

• 산업경영시스템학회지
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• 제22권52호
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• pp.241-246
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• 1999

In this study, to specify an evaluation of human sensibility, the types of color, intensity of illuminations and lights consisting work environmental condition are decided, and image data from examining the change of human sensibility followed by changes of the above three conditions are obtained. Using the factor analysis and quantification theory in multi-variate analysis type of Sensibility Ergonomics, determinating the structure of factors, specifying the relations of environmental conditions and factors can be done so that the structure of image on human sensibility space with the change of environmental conditions is analyzed.

• 제어로봇시스템학회논문지
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• 제9권8호
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• pp.610-615
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• 2003

This paper proposes a nonlinear virtual coupling fur haptic interface, which offers better performance while maintaining stability of the system. The nonlinear virtual coupling is designed based on a human response model. This human response model exploits delay between the human Intention and the actual change of arm impedance. The proposed approach provides with less conservative constraints for the design of stable haptic interface, compared with the traditional passivity condition. This allows increased performance that is verified through experiments.

• 대한한의진단학회지
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• 제18권3호
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• pp.137-148
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• 2014

Objectives This study was conducted to derive consensus about physical attributes in pressure pulse waveform and pulse conditions by Delphi study. Methods Delphi research was conducted for 2 rounds via e-mail. 8 Professors who lecture on a diagnostics of K. M. from the society of Korean medicine diagnostics were participated in this survey. They were asked for answering about series of definition for a physical attribute in pressure pulse waveform and combination for physical attributes of pulse conditions. Results 4 survey items were decided to have high validity and 9 survey items were decided to come to consensus about a physical attribute in pressure pulse waveform. 6 pulse condition were decided to come to consensus. Conclusion Using Delphi method, physical attributes in pressure pulse waveform and combinations of physical attribute in pulse condition come to consensus.

• 가정과삶의질연구
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• 제21권3호
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• pp.75-85
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• 2003

This study is aimed at sowing two purposes. The first is to analyze the real condition and demand of profession development from the human ecology majors. The second is to develop necessary methods in which ordinary people can collect necessary information about family life. Related questionnaire was distributed to 147 the human ecology majors, and analysis was performed on the several web sites providing with information related to family life. Web sites such as“www.yahoo.com”and“www.naver.com”were included in the analysis. Questionnaire consisted of questions about whether the students majoring in the human ecology were familiar with the information-related terms or futuristics-related books, and whether they have ever taken the related courses in college. The results of analysis are as follows: First, analysis shows that the present level of the human ecology majors' information orientation and networking experiences is extremely low. Secondly, according to the analysis on whether they have optimistic or pessimistic attitude toward the contemporay informatized society, the human ecology majors have rather optimistic attitude in group while having pessimistic one individually. As to their response to the question about whether informatized society is connected with industrial society or not, the human ecology majors are divided in neatly equal ratio. Thirdly, analysis of the human ecology majors' understanding of profession relevant to their major indicates that they have high level of perception and information about the professions of fashion designing, traditional garment designing, nutrition counselling or consumer counselling. On the other hand, they are not familiar with the information about professions such as professional QR Programming, eating habit-related information business and family welfare. Lastly, level of web sites supplying information about family life is fragmentary. Especially, probe into the directories providing with necessary information of family life subdivided into special areal of life shows that they lack systematic organization making more convenient consumer use.