• Journal of Veterinary Science
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• v.22 no.5
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• pp.59.1-59.13
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• 2021

Background: Antimicrobial peptides (AMPs) have been identified as promising compounds for consideration as novel antimicrobial agents. Objectives: This study analyzed the efficacy of cecropin B against Haemophilus parasuis isolates through scanning electron microscopy (SEM) and atomic force microscopy (AFM) experiments. Results: Cecropin B exhibited broad inhibition activity against 15 standard Haemophilus parasuis (HPS) strains and 5 of the clinical isolates had minimum inhibition concentrations (MICs) ranging from 2 to 16 ㎍/mL. Microelectrophoresis and hexadecane adsorption assays indicated that the more hydrophobic and the higher the isoelectric point (IEP) of the strain, the more sensitive it was to cecropin B. Through SEM, multiple blisters of various shapes and dents on the cell surface were observed. Protrusions and leakage were detected by AFM. Conclusions: Based on the results, cecropin B could inhibit HPS via a pore-forming mechanism by interacting with the cytoplasmic membrane of bacteria. Moreover, as cecropin B concentration increased, the bacteria membrane was more seriously damaged. Thus, cecropin B could be developed as an effective anti-HPS agent for use in clinical applications.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.10 no.9
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• pp.4342-4366
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• 2016

Congestion control in Cluster Wireless Sensor Networks (CWSNs) has drawn widespread attention and research interests. The increasing number of nodes and scale of networks cause more complex congestion control and management. Active Queue Management (AQM) is one of the major congestion control approaches in CWSNs, and Random Early Detection (RED) algorithm is commonly used to achieve high utilization in AQM. However, traditional RED algorithm depends exclusively on source-side control, which is insufficient to maintain efficiency and state stability. Specifically, when congestion occurs, deficiency of feedback will hinder the instability of the system. In this paper, we adopt the Additive-Increase Multiplicative-Decrease (AIMD) adjustment scheme and propose an improved RED algorithm by using neighbor feedback and scheduling scheme. The congestion control model is presented, which is a linear system with a non-linear feedback, and modeled by Lur'e type system. In the context of delayed Lur'e dynamical network, we adopt the concept of cluster synchronization and show that the congestion controlled system is able to achieve cluster synchronization. Sufficient conditions are derived by applying Lyapunov-Krasovskii functionals. Numerical examples are investigated to validate the effectiveness of the congestion control algorithm and the stability of the network.

• Nuclear Engineering and Technology
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• v.48 no.3
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• pp.744-750
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• 2016

Uranium removal from uranium plant wastewater using zero-valent iron in an ultrasonic field was investigated. Batch experiments designed by the response surface methodology (RSM) were conducted to study the effects of pH, ultrasonic reaction time, and dosage of zero-valent iron on uranium removal efficiency. From the experimental data obtained in this work, it was found that the ultrasonic method employing zero-valent iron powder effectively removes uranium from uranium plant wastewater with a uranium concentration of $2,772.23{\mu}g/L$. The pH ranges widely from 3 to 7 in the ultrasonic field, and the prediction model obtained by the RSM has good agreement with the experimental results.

• International Journal of Naval Architecture and Ocean Engineering
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• v.12 no.1
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• pp.414-427
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• 2020

The finite element method is used to simulate the navigation of an ice-area bulk carrier in broken ice fields. The ice material is defined as elastic, and the simulations are accomplished at four model speeds and three ice concentrations. The movements of ice floes in the simulation are consistent with those in the model test, and the percentage deviation of the numerical ice resistance from the ice resistance in the model test can be controlled to be less than 15 %. The key characteristics of ice loads, including the average ice loads, extreme ice loads, and characteristic frequency, are analyzed thoroughly in a comprehensive manner. Moreover, the effects of sailing speed and ice concentration on the ice loads are analyzed. In particular, the stress distribution of ice floes is presented to help understand how model speed and concentration affect the ice loads. The "ice pressure" phenomenon is observed at 90 % ice concentration, and it is realistically reflected both in the time―and frequency―domain ice force curves.

• Journal of Food Hygiene and Safety
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• v.26 no.1
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• pp.76-81
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• 2011

The protease produced by a Bacillus pumilus CN8 strain was purified by DEAE-Cellulose-52 ion exchange. It has a molecular weight of approximately 96,920 Dalton. In the present study, this protease showed strong activity over a broad range of pH (6.5-9.5) and temperature from $40^{\circ}C$ to $60^{\circ}C$, and the protease performed the maximal activity at pH 7.3 at $42^{\circ}C$. The effect of metal ions on protease activity showed that $K^+$ could slightly increase the protease activity, and other ions such as $Zn^{2+}$, $Fe^{2+}$, $Na^+$, $Ca^{2+}$, $Mg^{2+}$ had no significant activation or inhibition to the protease (P> 0.05), and the more important is that $Cu^{2+}$, $Mn^{2+}$, $Sn^{2+}$, $Cd^{2+}$ had a strong inhibitory effect on the protease activity.

• Horticultural Science & Technology
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• v.32 no.2
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• pp.261-268
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• 2014

Senescence of Hosta ventricosa flowers was firstly characterized as ethylene-sensitive since the deterioration of the tepal was accompanied by increased endogenous ethylene biosynthesis. The full-length cDNAs and DNAs of 1-aminocyclopropane-1-carboxylic acid (ACC) synthase (ACS) and ACC oxidase (ACO) involved in ethylene biosynthesis were cloned from H. ventricosa flowers. The HvACS ORF with 1347 bp and two introns, encoded a polypeptide of 448 amino acids showing 79% homology with that in Musa acuminata. The HvACO ORF contained 957 bp and three introns, encoding a 318-residue polypeptide showing 83% homology with that in Narcissus tazetta. The timing of the induction of HvACS expression was in correspond to the timing of the increase in ethylene production, and that the up-regulation of HvACO transcript was closely correlated with an elevated ethylene production, but underwent a down-regulation in wounded leaves with elevated ethylene emission. The results, together with expression analysis in vegetative tissues, suggested that both HvACS and HvACO were specifically regulated by flower senescence.

• Journal of Multimedia Information System
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• v.1 no.1
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• pp.55-67
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• 2014

Image topic and emotion analysis is an important component of online image retrieval, which nowadays has become very popular in the widely growing social media community. However, due to the gaps between images and texts, there is very limited work in literature to detect one image's Topics and Emotions in a unified framework, although topics and emotions are two levels of semantics that often work together to comprehensively describe one image. In this work, a unified model, Joint Topic/Emotion Multi-Modal Hierarchical Latent Dirichlet Allocation (JTE-MMHLDA) model, which extends previous LDA, mmLDA, and JST model to capture topic and emotion information at the same time from heterogeneous data, is proposed. Specifically, a two level graphical structured model is built to realize sharing topics and emotions among the whole document collection. The experimental results on a Flickr dataset indicate that the proposed model efficiently discovers images' topics and emotions, and significantly outperform the text-only system by 4.4%, vision-only system by 18.1% in topic detection, and outperforms the text-only system by 7.1%, vision-only system by 39.7% in emotion detection.

• Journal of the Korean Chemical Society
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• v.61 no.5
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• pp.231-237
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• 2017

Efficiently removing Al(III) from rare earth is very significant because even trace amount of Al(III) can cause serious harm to the rare earth materials. In this paper, a nitrogen-containing activated carbon, AC-P700, was synthesized using peas as raw materials. The AC-P700 was characterized by surface area analyzer, FT-IR, and XPS methods. The adsorption and recognition properties of AC-P700 towards Al(III) were investigated, and the recognition mechanism was also analyzed. The BET special surface area of AC-P700 was $1277.1m^2{\cdot}g^{-1}$, and the average pore diameter was 1.90 nm. The AC-P700 possesses strong adsorption affinity and excellent recognition selectivity towards Al(III). The adsorption capacity for Al(III) could reach to $0.53mmol{\cdot}g^{-1}$, and relative selectivity coefficients relative to La(III) and Ce(III) is 9.6 and 8.7, respectively. Besides, AC-P700 possesses better regeneration ability and reusability.

• Geomechanics and Engineering
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• v.20 no.6
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• pp.517-525
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• 2020

The long-term stability of rock engineering is significantly affected by the time-dependent deformation behavior of rock, which is an important mechanical property of rock for engineering design. Although the hard rocks show small creep deformation, it cannot be ignored under high-stress condition during deep excavation. The inner mechanism of creep is complicated, therefore, it is necessary to investigate the relationship between microscopic creep mechanism and the macro creep behavior of rock. Microscopic numerical modeling of sandstone creep was performed in the investigation. A numerical sandstone sample was generated and Parallel Bond contact and Burger's contact model were assigned to the contacts between particles in DEM simulation. Sensitivity analysis of the microscopic creep parameters was conducted to explore how microscopic parameters affect the macroscopic creep deformation. The results show that the microscopic creep parameters have linear correlations with the corresponding macroscopic creep parameters, whereas the friction coefficient shows power function with peak strength and Young's modulus, respectively. Moreover, the microscopic parameters were calibrated. The creep modeling curve is in good agreement with the verification test result. Finally, the creep curves under one-step loading and multi-step loading were compared. This investigation can act as a helpful reference for modeling rock creep behavior from a microscopic mechanism perspective.

• Geomechanics and Engineering
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• v.7 no.2
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• pp.165-181
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• 2014

In the past decade, different types of underreamed ground anchors have been developed for substructures requiring uplift resistance. This article introduces a new type of umbrella-shaped anchor. The uplift behavior of this ground anchor in clay is studied through a series of laboratory and field uplift tests. The test results show that the umbrella-shaped anchor has higher uplift capacity than conventional anchors. The failure mode of the umbrella-shaped anchor in a large embedment depth can be characterized by an arc failure surface and the dimension of the plastic zone depends on the anchor diameter. The anchor diameter and embedment depth have significant influence on the uplift behavior. A finite element model is established to simulate the pullout of the ground anchor. A parametric study using this model is conducted to study the effects of the elastic modulus, cohesion, and friction angle of soils on the load-displacement relationship of the ground anchor. It is found that the larger the elastic modulus and the shear strength parameters, the higher the uplift capacity of the ground anchor. It is suggested that in engineering design, the soil with stiffer modulus and higher shear strength should be selected as the bearing stratum of this type of anchor.