• Korean Institute of Interior Design Journal
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• v.14 no.5 s.52
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• pp.226-235
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• 2005

Since the late 20th century, digital technology has changed our environment in many ways. The representative advanced homes today embed digital facilities such as a sensor infrastructure, a networking system, and ubiquitous display. In this point of view, this study was intended to define the apartment residents' diverse needs for digital home design. The subjects are 400 wives who live in Busan. The findings are as follows; (1) The factors of the housing activities are extracted as 'guest hosting,' 'family entertaining,' 'digital network,' 'daily domestic affair,' 'non-daily domestic affair,' 'media using,' and 'bathing & exercising' activities. (2) The 'digital network activities' occurred more frequently in the households with non-employed wife and preschool children, and with employed wife and preschool and elementary school children. (3) The factors of the digital home system were extracted as 'safety & maintenance,' 'health & convenience,' 'educational & housekeeping,' and 'cultural & entertaining' systems. (4) The needs of non-employed wives for the safety & maintenance and the educational & housekeeping systems are varied by family life cycle and wife age. While, the needs of employed wives for the educational & housekeeping and the culture & entertaining systems are varied by dwelling-size and wife age.

• Genomics & Informatics
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• v.11 no.4
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• pp.272-276
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• 2013

Sequence analysis of the 16S rRNA gene has been widely used for the classification of microorganisms. However, we have been unable to clearly identify five Flavobacterium species isolated from a freshwater by using the gene as a single marker, because the evolutionary history is incomplete and the pace of DNA substitutions is relatively rapid in the bacteria. In this study, we tried to classify Flavobacterium species through multilocus sequence analysis (MLSA), which is a practical and reliable technique for the identification or classification of bacteria. The five Flavobacterium species isolated from freshwater and 37 other strains were classified based on six housekeeping genes: gyrB, dnaK, tuf, murG, atpA, and glyA. The genes were amplified by PCR and subjected to DNA sequencing. Based on the combined DNA sequence (4,412 bp) of the six housekeeping genes, we analyzed the phylogenetic relationship among the Flavobacterium species. The results indicated that MLSA, based on the six housekeeping genes, is a trustworthy method for the identification of closely related Flavobacterium species.

• Journal of Korean Academy of Nursing
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• v.7 no.2
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• pp.22-30
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• 1977

An analysis of the Nursing activities was carried out during the period of October 6 - 10, 1975 for five days, at postanesthetic room in Seoul National University Hospital. Continuous Time Study method was applied to this study by Observing and checking the activities performed by 8 nursing personnel at head nurse, staff nurse, and nurse aide level. Six nursing Students and 6 staff nurses observed and recorded all activities during the day and the evening for 5 days after certain process of training. Following results were obtained I 1. Percentages of nursing activity os were identified at each level of nursing personnel according to the skill Level 1) Thirty three point forty two Percent of the total head nurse's activities were spent by administrative activities, 21,33% by Clerical activities, 10.63% by Nursing activities, 6.54% by Messenger activities, 4.0% by Housekeeping activities, and 24. 08% by Unclassified activities, 2) Forty point forty two percent of the total staff nurses'activities were spent by Nursing activities, 12.7% by Administrative activities, 8.03% by Housekeeping activities, 3.08% by Clerical activities, 2.03% by Messenger activities, 0.08% by Dietary activities, and 34.19 o/e by Unclassified activities. 3) Thirty six point sixty three percent of the total nurse aide's activities were spent by Messenger activities, 14.4% by Housekeeping activities, 2.2% by Nursing activities, 1.0% by Clerical activities, 0.83% by Administrative activities, and 44.94% by Unclassified activities, 2. Percentages of nursing activities were identified at each level of nursing personnel according to area of activity. 1) Thirty three point ninety six percent of the total head nurse's activities were spent by Unit - Centered activities, 30.26% by Patient - Centered activities, 8.69 % by Personnel - Centered activities and 24.09 o/o by Other - Centered activities. 2) Fifty two point seventy four percent of the total staff nurses' activities were spent by Patient - Centered activities, 11.5 % by Unit -Centered activities, 1.68 % by Personnel -Centered activities and 34.02% by Other - Centered activities. 3) Forty nine point sixty seven Percent of the total Nurse aide's activities were spent by Unit -Centered activities, 5.13% by Patient -Centered activities, 0.27% by Personnel -Centered activities and 44.93%by Other -Centered activities. 3. Percentages of staff nurses' activities were identified at each skill Level according to their shifts. 1) Forty four point eighty one percent of the total day time activities were spent by Nursing activities, 13.62% by Administrative activities, 6.37% by Housekeeping activities, 2.08% by Clerical activities, 1.74 % by Messenger activities, 0.07% by Dietary activities and31.31 o/o by Unclassified activities. 2) Thirty three point eighty seven percent of the total evening time activities were spent by nursing activities, 10.51% by Housekeeping activities, 10.0% by Administrative activities, 4.58% by Clerical activities, 2.46% by Messenger activities, 0.09% by Dietary activities and 38.49% by Unclassified activities, 4. There was no great difference among activities of 5 days.

• BMB Reports
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• v.44 no.4
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• pp.250-255
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• 2011

In multicellular organisms, including humans, understanding expression specificity at the tissue level is essential for interpreting protein function, such as tissue differentiation. We developed a prediction approach via generated sequence features from overrepresented patterns in housekeeping (HK) and tissue-specific (TS) genes to classify TS expression in humans. Using TS domains and transcriptional factor binding sites (TFBSs), sequence characteristics were used as indices of expressed tissues in a Random Forest algorithm by scoring exclusive patterns considering the biological intuition; TFBSs regulate gene expression, and the domains reflect the functional specificity of a TS gene. Our proposed approach displayed better performance than previous attempts and was validated using computational and experimental methods.

• Biomedical Science Letters
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• v.11 no.3
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• pp.289-293
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• 2005

Two housekeeping genes, of Listeria monocytogenes dat and hlyA, were analyzed in a set of 28 isolates from different sources to estimate their genetic diversities. These strains were previously characterized by pulsed-field gel electrophoresis. Complete gene sequences for dat (465 bp) and hlyA (584 bp) had sequence similarity of $99.87-100\%$ S and $99.96-100\%$ S among isolates, respectively. Also, we found that the numbers of sequence types (ST) were about 3-fold less than those of PFGE types (3 STs versus 11 PFGE types). There was, however, a good correlation between the PFGE patterns and phylogenetic grouping of two gene sequences among the isolates. Further studies on analyzing additional loci would increase the discriminatory power of sequence typing for L. monocytogenes strains.

• Journal of Microbiology and Biotechnology
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• v.32 no.8
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• pp.1011-1016
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• 2022

Bacillus subtilis is a useful bacterium in the food industry with applications as a starter strain for fermented food and as a probiotic. However, it is difficult to discriminate B. subtilis from other Bacillus species because of high phenotypic and genetic similarity. In this study, we employed five previously constructed multilocus sequence typing (MLST) methods for the discrimination of B. subtilis from other Bacillus species and all five MLST assays clearly distinguished B. subtilis. Additionally, the 17 housekeeping genes used in the five MLST assays also clearly distinguished B. subtilis. The pyruvate carboxylase (pyrA) and shikimate dehydrogenase (aroE) genes were selected for the discrimination of B. subtilis because of their high number of polymorphic sites and the fact that they displayed the lowest homology among the 17 housekeeping genes. Specific primer sets for the pyrA and aroE genes were designed and PCR products were specifically amplified from B. subtilis, demonstrating the high specificity of the two housekeeping genes for B. subtilis. This species-specific PCR method provides a quick, simple, powerful, and reliable alternative to conventional methods in the detection and identification of B. subtilis.

• Journal of fish pathology
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• v.30 no.2
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• pp.79-88
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• 2017

At the present, fish farms are suffering a lot of economic losses due to infectious diseases caused by various pathogens including aeromonad. Aeromonad is ubiquitous bacteria that causes infectious diseases. At least 26 species in the genus Aeromonas have been reported to cause fatal infections not only in salmonid fishes, but also in other freshwater and seawater fishes. Molecular techniques based on nucleic acid sequences of 16S rDNA and housekeeping genes can be used to identify the Aeromonas species. In this study, The genus Aeromonas was isolated from salmonid fishes of sixteen fish farms in Gangwon-Do, Korea and phylogenetically identified based on the sequences of 16S rDNA and housekeeping genes for Aeromonad, i.e. RNA polymerase sigma factor ${\sigma}^{70}$ (rpoD) or DNA gyrase subunit B (gyrB). Consequently, 96 strains were collected from Atlantic salmon (Salmo salar), coho salmon (Oncorhynchus kisutch), masou salmon (Oncorhynchus masou) and rainbow trout (Oncorhynchus mykiss), and 36 isolates were identified as the genus Aeromonas by 16S rDNA analysis. Thirty six Aeromonad isolates were further analysed based on rpoD or gyrB gene sequences and found Aeromonas salmonicida (24 isolates), A. sobria (10 isolates), A. media (1 isolates) and A. popoffii (1 isolates), indicating that A. salmonicida is a main infectious bacteria in Salmonid fishes in Gangwon-Do. It was also proved that the phylogenetic identification of Aeromonas species based on the sequences of housekeeping gene is more precise than the 16S rDNA sequence.

• Proceedings of the Korean Society of Postharvest Science and Technology of Agricultural Products Conference
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• 2007.11a
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• pp.219.1-219.1
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• 2007

• Journal of the Korean Home Economics Association
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• v.28 no.3
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• pp.105-125
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• 1990

The 78 housekeeping books were analyzed to find out the structure of income and expenditure of the farm household. The selected farm households were classified into 4 different farming types such as rice-cultivating, vinyl house, fruit-growing, and livestock farming. The results are summarized as follows : 1) The farm housekeeper ought to rationally manage farm household money income, because agricultural income was preponderated to several months regardless of farming types. 2) Farm household income was primarily dependent upon agricultural income and non-agricultural income in the livestock farming and rice-cultivating farm houshold respectively. 3) order of living expenses of the total farm households were recreation and entertainment expenses, food expenses, education expenses, and housing, fuel & light expenses in size. The major expenses were education expenses, food expenses and miscellaneous expenses in rice-cultivating, vinyl house and livestock farming, and fruit growing farm households respectively. 4) Balance of income and expenses of the farm household, s its time, size, and pattern of increase and decrease, was different by farming types. 5) Household expenses increased in February, May, August and December, though disposable income reversely decreased in February, April, August and December compared to each former month. So, special consideration should be taken into budget planning for household money management in February, August and December.

• Aerospace Engineering and Technology
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• v.6 no.2
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• pp.87-95
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• 2007

The ocean and meteorological payloads of COMS are concerned to experience degration of image quality due to the disturbance induced by the motion of moving parts of the payloads. And thruster firings for stationkeeping and wheel offloading are expected to degrade the image quality of the optical payloads. In case of COMS, in order to keep the optical payload free from the mechanical interference from the other payload, the operation design approach has been taken. This paper introduces the operation design of COMS taken to avoid these problems. In order to meet users requirement by avoiding the degradation of image quality, the timeline of optical payloads and housekeeping are optimized, and operational constraints are applied to the mirror motion of the meteorological payload. This paper also introduces the results of time budget analysis performed to validate the operation design.