• Journal of Animal Reproduction and Biotechnology
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• v.34 no.4
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• pp.280-291
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• 2019

Potential utility of 14 candidate housekeeping genes as normalization reference for RT-qPCR analysis with developmental samples (fertilized eggs to late veliger larvae) in Pacific abalone Haliotis discus hannai was evaluated using four different statistical algorithms (geNorm, NormFinder, BestKeeper and comparative ΔCT method). Different algorithms identified different genes as the best candidates, and geometric mean-based final ranking from the most to the least stable expression was as follow: RPL5, RPL4, RPS18, RPL8, RPL7, UBE2, RPL7A, GAPDH, RPL36, PPIB, EF1A, ACTB and B-TU. The findings were further validated via relative quantification of metallothionein (MT) transcripts using the stable and unstable reference genes, and expression levels of MT were greatly influenced according to the choice of reference genes. In overall, our data suggest that RPL5 and RPS18, either singly or in combination, are appropriate for normalizing gene expression in developmental samples of this abalone species, whereas ACTB, B-TU and EF1A are less stable and not recommended. In addition, our findings propose that standard deviations in geometric ranking as well as geometric mean itself should also be taken into account for the final selection of reference gene(s). This study could be a useful basis to facilitate the generation of accurate and reliable RT-qPCR data with developmental samples in this abalone species.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.28 no.2
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• pp.189-196
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• 2010

Spatial information and smart phone technology have made innovative improvement of daily life. Spatial and geographic information are in practice for various applications. Especially, spatial information along with information and telecommunication technology could create new contents for providing services for convenient daily life. Spatial information technology, recently, is not only for acquiring location and attribute data but also providing tools to extract information and knowledge systematically for decision making. Various indoor applications have emerged in accordance with demands on daily GIS(Geographic information system). This paper aims for applying spatial information technology to support decision-making in shopping. The main contents include product database, optimal path search, shopping time expectation, automatic housekeeping book generation and analysis. Especially for foods, function to analyze information of the nutrition facts could help to improve dietary pattern and well-being. In addition, this system is expected to provide information for preventing overconsumption and impulse purchase could help economical and effective purchase pattern by analyzing propensity to consume.

• Korean Journal of Environmental Biology
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• v.29 no.1
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• pp.1-10
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• 2011

Amphibian population declines globally. Aquatic contamination by organic pollutants including endocrine disrupters has been suspected to the one of the reason for distinction of amphibia which has obligate aquatic life style during larval period. Amphibians have been widely accepted as animal model for the study of endocrine disruption in aquatic ecosystem at molecular as well as individual levels. There are increasing need for toxicological data in amphibians at multiple endpoints for management of contamination and development of safety guideline for important EDs in aquatic media. Alkylphenols have been widely used in agricultural, industrial, and housekeeping activities, contaminating the aquatic media and evoking endocrine disruption in aquatic animals. In this review, we summarized data concerning the endocrine disruption by alkylphenol organic pollutants on amphibians according to route, concentration, terms, and developmental stage of exposure together with mechanism of endocrine disruption.

• Journal of Digital Convergence
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• v.11 no.3
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• pp.1-12
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• 2013

Present study examines the gender disparity in terms of its beneficiaries or benefit amount of National Pension of South Korea from the perspective of gender sensitivity. National Pension system has been manipulated and developed in order to maximize its universality. However, substantial gender differences are still found in terms of beneficiary number and benefit amount in the program. Benefit condition and benefit structure are determined assuming that male is the primary breadwinner in household and the primary regular full time worker in labor market. Women are only counted as dependents or excluded as unstable workers. As a result, women are fully or partially excluded from the program as they are excluded in other public sector such as labor market. Women's work (such as caring and housekeeping) are not taken into account in National Pension program. Policy suggestions for the National Pension of South Korea are also provided as the last part of this paper.

• Restorative Dentistry and Endodontics
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• v.40 no.4
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• pp.306-313
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• 2015

Objectives: Molecular mechanism of the pathogenicity of Enterococcus faecalis (E. faecalis), a suspected endodontic pathogen, has not yet been adequately elucidated due to limited information on its virulence factors. Here we report the identification of in vivo expressed antigens of E. faecalis by using a novel immunoscreening technique called change-mediated antigen technology (CMAT) and an experimental animal model of endodontic infection. Materials and Methods: Among 4,500 E. coli recombinant clones screened, 19 positive clones reacted reproducibly with hyperimmune sera obtained from rabbits immunized with E. faecalis cells isolated from an experimental endodontic infection. DNA sequences from 16 of these in vivo-induced (IVI) genes were determined. Results: Identified protein antigens of E. faecalis included enzymes involved in housekeeping functions, copper resistance protein, putative outer membrane proteins, and proteins of unknown function. Conclusions: In vivo expressed antigens of E. faecalis could be identified by using a novel immune-screening technique CMAT and an experimental animal model of endodontic infection. Detailed analysis of these IVI genes will lead to a better understanding of the molecular mechanisms involved in the endodontic infection of E. faecalis.

• Journal of Microbiology and Biotechnology
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• v.22 no.5
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• pp.575-584
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• 2012

Listeria monocytogenes is an important foodborne pathogen that comprises four genetic lineages: I, II, III, and IV. Of these, lineage II is frequently recovered from foods and environments and responsible for the increasing incidence of human listeriosis. In this study, the phylogenetic structure of lineage II was determined through sequencing analysis of the ascB-dapE internalin cluster. Fifteen sequence types proposed by multilocus sequence typing based on nine housekeeping genes were grouped into three distinct sublineages, IIA, IIB, and IIC. Organization of the ascB-dapE internalin cluster could serve as a molecular marker for these sublineages, with inlGHE, inlGC2DE, and inlC2DE for IIA, IIB, and IIC, respectively. These sublineages displayed specific genetic and phenotypic characteristics. IIA and IIC showed a higher frequency of recombination (${\rho}/{\theta}$). However, recombination events had greater effect (r/m) on IIB, leading to its high nucleotide diversity. Moreover, IIA and IIB harbored a wider range of internalin and stress-response genes, and possessed higher nisin tolerance, whereas IIC contained the largest portion of low-virulent strains owing to premature stop codons in inlA. The results of this study indicate that IIA, IIB, and IIC might occupy different ecological niches, and IIB might have a better adaptation to a broad range of environmental niches.

• The Bulletin of The Korean Astronomical Society
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• v.39 no.1
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• pp.54.2-54.2
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• 2014

IGRINS (Immersion GRating Infrared Spectrograph) is a high spectral resolution near-infrared spectrograph that has been developed in a collaboration between the Korea Astronomy & Space Science Institute and the University of Texas at Austin. By using a silicon immersion echelle grating, the size of the fore optics is reduced by a factor of three times and we can make a more compact instrument. One exposure covers the whole of the H- and K-band spectrum with R=40,000. While the operation of and data reduction for this instrument is relatively simple compared to other grating spectrographs, we still need to operate three infrared arrays, cryostat sensors, calibration lamp units, and the telescope during astronomical observations. The IGRINS Instrument Control Software consists of a Housekeeping Package (HKP), Slit Camera Package (SCP), Data Taking Package (DTP), and Quick Look Package (QLP). The SCP will do auto guiding using a center finding algorithm. The DTP will take the echellogram images of the H and K bands, and the QLP will confirm fast processing of data. We will have a commissioning observations in 2014 March. In this poster, we present the performance of the software during the test observations.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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• 2017.05a
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• pp.413-416
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• 2017

The development of the internet, which is due to the advent of the fourth industrial revolution, has been slowly affecting our lives. Based on this trend, various products have recently emerged, and have yet to be developed in the context of identifying the dangers of infant babies. Increasingly, children are experiencing problems with detecting and responding to children's lives because of their daily living noise and housekeeping activities. This project attempts to develop a raspberry pie and an audiovisual sensor module to avoid the risk of preventing unwanted behavior from tripping the child's sudden behavior in everyday life. Further, it was designed to provide convenience to the guardian's convenience by implementing the smartphone app with the Wifi signal.

• Journal of the Ergonomics Society of Korea
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• v.26 no.2
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• pp.67-79
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• 2007

The objective of this study is to investigate a perceived discomfort and cause of Korean elderly in various activities such as work and leisure as well as in daily activities. 120 subjects (30 female and 30 male for each age: the young and elderly) participated in the interview type of questionnaire. Each participant answered to 41 items of activities of daily living. The examination of each item was progressed with 3 steps: whether to do the activity or not, degree of activity discomfort, and the reasons or causes of discomfort. Borg's CR10 scale is used to measure the degree of discomfort of daily activities. As a result, there are differences for the proportion of performers between two age groups, except for essential activities such as individual maintenance and housekeeping. The elderly complained about their daily activities more than the young did, and the degree of discomfort of women was significantly larger than that of men with aging. There are seven items, the mean degree of discomfort of which is over three points for the elderly, and they showed significant difference between two age groups. It was the important reason that physical capacities such as muscle strength and suppleness are declined. The results showed the characteristics of Korean elderly in the whole daily activities. These will be used to understand various life styles of them and to propose a field or direction of study related to the aging.

• Journal of Families and Better Life
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• v.23 no.3 s.75
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• pp.153-167
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• 2005

This study was conducted to help develop women's human resources and promote married women's return to workforce. Using various data, we examined the patterns of Korean women's economic activities over the lifecycle, career discontinuation and return to the workforce. We also interviewed twenty full-time housewives who wanted to return to workforce. The interview questions included reasons for seeking employment, desired working conditions, and difficulties in finding a job. Major findings are as follows: First, there were two groups with respect to reasons why they want employment One was an economic need such as earning basic living expenses and supporting the family. The other was self-realization and social participation. Second most women wanted to do unskilled labor such as housekeeping work, whereas some young or well-educated women looked for a professional job. Third, married women preferred part-time jobs, which were compatible with their family care demands. Most of them wanted a workplace located close to home. The women with child-care responsibility preferred working at home. Fourth, regarding difficulties of returning to workforce, they pointed out vocational ability problems due to their career discontinuation, social prejudice such as gender discrimination and psychological pressure in maintaining work and family at the same time. In order to promote employment of married women, it is required not only to change social prejudices but also to increase effectiveness in policy implementation. In addition, counseling for job-search and vocational ability training programs should be provide.