• 대한안전경영과학회지
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• 제6권2호
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• pp.11-21
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• 2004

Objectives: The aim of this study is to determine prevalence and related factors of musculoskeletal symptoms in electronics assembly line workers. Method: A questionnaire survey was performed on 350 workers in In-chon city. The questionnaire consisted of questions on the general characteristics, work duration, working hours, hobby activity hours, housekeeping activity hours and standardized descriptions of NOISH on musculoskeletal symptoms. Result: Complaint rates of musculoskeletal symptoms by anatomical site were as follows: shoulder, 67% : neck, 40.4% : waist, 28.7% : wrist and hand, 27.1% : foot and leg, 14.4% : arm and elbow, 11.2%. Significantly higher prevalence of shoulder musculoskeletal symptom was found women than men, married than unmarried, less than 1hours housekeeping activity than over 1hours housekeeping activity. Multiple logistic regression analysis identified gender, marital status and housekeeping activity as the main affecting factors for shoulder musculoskeletal symptoms. Conclusions: The most prevalence of musculoskeletal symptoms was shoulder site. It was higher in married women and over 1hours housekeeping activity in a day. It is recommended that married women should be reduce housekeeping activity time.

• 한국작물학회지
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• 제55권4호
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• pp.371-378
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• 2010

For sensitive and accurate gene expression analysis, normalization of gene expression data against housekeeping genes is required. There are conventional housekeeping gene (e.g. ACT) that primarily function as an internal control of transcription. In this study, we performed an in silico analysis of 278 rice gene expression samples (GSM) in order to identify the gene that is most consistently expressed. Based on this analysis, we identified novel candidate housekeeping genes that displayed improved stability among the cross experimental conditions. Furthermore four of the most conventional housekeeping genes were included in our 30 other housekeeping genes among the most stable genes. Therefore, these 30 genes can he used to normalize transcription results in gene expression studies on rice at a broad range of experimental conditions.

• BMB Reports
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• 제40권2호
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• pp.226-231
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• 2007

Housekeeping genes are widely used as internal controls in a variety of study types, including real time RT-PCR, microarrays, Northern analysis and RNase protection assays. However, even commonly used housekeeping genes may vary in stability depending on the cell type or disease being studied. Thus, it is necessary to identify additional housekeeping-type genes that show sample-independent stability. Here, we used statistical analysis to examine a large human microarray database, seeking genes that were stably expressed in various tissues, disease states and cell lines. We further selected genes that were expressed at different levels, because reference and target genes should be present in similar copy numbers to achieve reliable quantitative results. Real time RT-PCR amplification of three newly identified reference genes, CGI-119, CTBP1 and GOLGAl, alongside three well-known housekeeping genes, B2M, GAPD, and TUBB, confirmed that the newly identified genes were more stably expressed in individual samples with similar ranges. These results collectively suggest that statistical analysis of microarray data can be used to identify new candidate housekeeping genes showing consistent expression across tissues and diseases. Our analysis identified three novel candidate housekeeping genes (CGI-119, GOLGA1, and CTBP1) that could prove useful for normalization across a variety of RNA-based techniques.

• 한국식품저장유통학회지
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• 제15권4호
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• pp.562-567
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• 2008

GM 및 non-GM 감자의 인체 위해성 여부를 판단하기 위하여 마우스 비장세포에서 인터루킨과 housekeeping gene의 발현을 RT-PCR로 비교 분석하였다. 유전자변형 감자의 잎과 이를 섭취한 마우스의 비장세포에서 발현되는 housekeeping gene의 발현을 수행한 결과, APRT, $\beta$-tubulin, Actin, Hsp 20.2, Cyclophilin, 18S RNA and Efla, and Tbp, GAPDH, $\beta$-actin, Tuba2, Hprt, Cyclophlin A, Tfrc, and RPL13A의 발현에는 유전자 변형 감자와 그렇지 않은 감자와의 차이를 발견할 수 없었다. GM작물의 안전성에 대한 연구는 많은 표본을 가지고, 보다 장기적인 관점에서 다양한 모니터링 시스템을 도입이 필요하다고 판단되며 이의 향후 연구에 많은 관심이 필요하다.

• 한국식품저장유통학회지
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• 제14권6호
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• pp.688-694
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• 2007

To evaluate the human risk of long-term intake of genetically modified (GM) rice, we carried out RT-PCR of housekeeping genes. Housekeeping genes, which show highly uniform expression in living organisms during various stages of development and under different environmental conditions, were normalized by RT-PCR. We assessed the expression of 10 common housekeeping genes (18s rRNA, 25S rRNA, UBC, UBQ5, UBQ10, ACT11, GAPDH, eEF-$1{\alpha}$, ${\beta}$-TUB, GAPDH, ${\beta}$-actin, B2m, G6pd2, Gyk, Gus, Hprt, Cyclophlin A, Tfrc, ${\alpha}$-tubulin and RPL13A) in the liver, stomach, small intestine, large intestine, kidney and spleen of mice fed GM or non-GM rice. We found no significant differences in the expression of housekeeping genes between the two groups of mice.

• 대한가정학회지
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• 제31권1호
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• pp.225-239
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• 1993

The purpose of this study is to look for the basic formative elements of housekeeping space of our cultural life which are worthy to succeed and develop by revaluating in modern view and to reveal what we should maintain form the women's view of the housework in those days. In this study, out look on Chosun Dynasty housekeeping is based on the norm of the women in those days. The research on the housekeeping space for norm of the women in those days. the research on the housekeeping space for Chosun Dynasty upperclass is focused on the house built from early till late Chosun Dynasty, which is now a cultural asset. As a result, norm of Chosun Dynasty have created a social wide atmosphere for women to devote herselves to the housekeeping. Moreover the constructions of Chosun Dynasty's upperclass are affected by Korea's natural environment and socio-cultural environment. Primary factors for natural environment are building materials and the change of seasons. Because of changeable seasons, there were places for storing massive commodities. The other primary factors for socio-cultural environment affecting housekeeping space were social positions, and extended family system, worshipping the ancestors standard of living, the theory of“Poong Soo”(風水, a kind of geomancy) and thought of“Yin and Yang, Five Elements”(陰陽五行) and popular belief of that time. Affected by these two environments, the characteristics of housekeeping space for Chosun Dynasty are ; the main building of the house was used as a diverted area for women to work, and was reconstructed for household affairs. There were inefficient points in the housekeeping space of that time and the space remained stagnant because of the restricted sex and the social position of housekeeping doer. But you could find some points that are efficient and functional to adjust in those living conditions. The storehouse, storage barn, shed and soyjar terrace are the specialized storing place, according to the kinds and characteristics of the commodities. the kitchen, for example, is the combinations of many abilities. It is not only for cooking or heating the room, but also for storing drinking water. The hall which was the storing place, housekeeping place and also the place for family events are used as a diverted place. The linked arrangement of kitchen, store house, boudoir and the other rooms. In addition, we can see that the construction of the kitchen for more than tow people could work in.

• 산업공학
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• 제15권1호
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• pp.82-88
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• 2002

A study was conducted to develop user-centered kitchen furniture and workspace to reduce fatigue and to prevent housewives's disorders such as low back pain and musculoskeletal disorders from housekeeping activities. A questionnaire survey and interview were performed on 150 households those live in 32Pyung-type($105m^2$) apartment in Seoul vicinity. Also actual housekeeping activities for 24 hours were video-taped for further motion analysis. Results of the study revealed that housewives complained the inconvenience and small size of storage space, and worksurface height as the most important problems to be considered for redesign. And they responded, if feasible, sitting is preferred over standing while they are working. After adapting a sit/stand chair in the kitchen, a physiological experiment measuring heart rate(HR) and oxygen consumption($VO_2$) as response variables was conducted to examine the effects of sit/stand chair in reducing physiological demand during housekeeping activities. The results showed that working on sit/stand chair reduced energy expenditures by maximum of 30% and 31.0% in terms of HR and $VO_2$, respectively. Also rearrangement of kitchen structure based on motion analysis showed that walking distance during daily housekeeping activities can be reduced by 5.5% on the average. Hence, it is concluded that adapting a sit/stand chair in the kitchen could reduce fatigue and occupational disorders of housewives from extended housekeeping activities.

• 한국식품저장유통학회지
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• 제15권1호
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• pp.84-87
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• 2008

유전자 변형 작물은 생산성 측면에서 많은 장점이 있지만 이를 섭취할 경우 잠재적인 위험 요소들에 의해 많은 문제가 대두대고 있다. 본 연구는 저항성유전자를 이입한 배추에서 Profillin, Tubulin-${\alpha}$ (Tub-${\alpha}1$), Heat-shock protein (Bchsp 17.6) and Ubiquitin conjugating enzyme (UBE)의 발현과 이를 30일간 섭취한 마우스에서 ${\beta}$-actin(${\beta}$-act), ${\beta}$-2-microglobulin (B2m), Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and ${\beta}$-glucuronidase (Gus)의 발현 정도를 RT-PCR을 통해 알아보았다. 실험 결과 저항성유전자를 이입한 배추와 그렇지 않은 배추의 유전자 발현 패턴은 큰 차이를 보이지 앓았으며, 이를 섭취한 마우스 장기에서도 발현에 따른 큰 차이는 나타나지 않았다.

• 한국지능시스템학회논문지
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• 제26권5호
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• pp.396-401
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• 2016

사물인터넷이 발전함에 따라 다양한 분야에서 적용될 수 있는 제품들이 개발되고 있고, 여러 연구들이 진행되고 있다. 사물인터넷의 여러 분야 중 스마트 홈은 실생활과 밀접하기 때문에 다른 분야보다 주목받고 있다. 자율이동로봇 또한 산업과 군사, 가정 등에서 적용되어 여러 역할을 수행하고 있다. 본 논문에서는 자율이동로봇과 사물인터넷을 결합하여 Smart housekeeping 로봇을 구현하였다. Smart housekeeping 로봇을 구현하기 위해 라즈베리 파이와 무선 USB 카메라, Huins 사의 uBrain 로봇을 사용하였다. 로봇을 제어하기 위해 핸드폰을 라즈베리 파이의 IP에 접속하였고 라즈베리 파이에서는 uBrain 로봇과 블루투스 연결을 하였다. 핸드폰에서 해당 명령에 맞춰 로봇을 제어하도록 구현하였다. 또한 사용자가 원할 경우 로봇이 자율 주행을 선택할 수 있도록 구현하였다. 무선 USB 카메라로 실시간 촬영하는 영상을 핸드폰 혹은 개인용 컴퓨터로 확인할 수 있도록 하였다. 이 Smart housekeeping 로봇은 집 내부를 실시간으로 확인할 수 있도록 도울 것이다.

• 한국산학기술학회논문지
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• 제21권8호
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• pp.417-428
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• 2020

하우스키핑 유전자는 에너지생성, 물질합성, 세포사멸 및 세포방어 등과 같은 세포의 기본적인 기능을 수행하기 때문에 모든 유기체의 세포에서 발현된다. 세포의 기본적인 기능을 유지하기 때문에 발현 수준이 상대적으로 일정하여 단백질 발현 및 목적 유전자의 mRNA 발현 분석 등과 같은 유전자 발현 연구에서 기준 유전자로 사용되고 있다. 그러나 이들 유전자의 발현 수준은 조직과 세포마다 다를 수 있으며, 특정 환경 하에서 변할 수 있다. 그러므로 하우스키핑 유전자의 발현 안정성을 탐색하여 유전자 발현 연구에서 최적의 기준 유전자를 선택하는 것이 중요하다. 이 리뷰는 문헌을 통해 인간, 닭, 돼지 그리고 쥐에서 발견된 하우스키핑 유전자를 요약하고, geNorm, NormFinder 그리고 BestKeeper 소프트웨어를 통해 발현 안정성을 추정하였다. 하우스키핑 유전자의 발현 안정성에 대한 탐색은 유전자 발현 연구에서 실험 조건에 따라 가장 적합한 기준 유전자를 선별할 수 있고, 데이터의 정규화를 위해 적용될 수 있다.