• Journal of Plant Biotechnology
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• v.45 no.3
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• pp.228-235
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• 2018

In this study, two pepper varieties, PRH1 (powdery mildew resistance line) and Saengryeg (powdery mildew resistance line), were resequenced using next generation sequencing technology in order to develop InDel markers. The genome-wide discovery of InDel variation was performed by comparing the whole-genome resequencing data of two pepper varieties to the Capsicum annuum cv. CM334 reference genome. A total of 334,236 and 318,256 InDels were identified in PRH1 and Saengryeg, respectively. The greatest number of homozygous InDels were discovered on chromosome 1 in PRH1 (24,954) and on chromosome 10 (29,552) in Saengryeg. Among these homozygous InDels, 19,094 and 4,885 InDels were distributed in the genic regions of PRH1 and Saengryeg, respectively, and 198,570 and 183,468 InDels were distributed in the intergenic regions. We have identified 197,821 polymorphic InDels between PRH1 and Saengryeg. A total of 11,697 primers sets were generated, resulting in the discovery of four polymorphic InDel markers. These new markers will be utilized in order to identify disease resistance genotypes in breeding populations. Therefore, our results will make a one-step advancement in whole genome resequencing and add genetic resource datasets in pepper breeding research.

• Journal of Forest and Environmental Science
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• v.31 no.4
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• pp.272-279
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• 2015

Xanthoceras sorbifolium is considered as bio-energy crops owing to the high oil content from kernel. This study was performed to analyze calorific value, crude ash content, ultimate ratio, crude lipid and fatty acid composition among seed sources. Calorific values ranged from $4,526.0\;cal\;g^{-1}$ to $7,377.2\;cal\;g^{-1}$ in seeds and kernels showed the highest value. Calorific values and crude ash contents were observed as significant difference among plantations and/or individuals (p>0.05). Kernel from SD-F plantation showed the highest calorific value and lower crude ash content. C content comprised 63.4%, the highest levels was detected from SD-F (64.8%). Crude lipid content in kernel observed as 54.5 g $100\;g^{-1}$ from SD-F. In contrast it was determined the lowest value from LN-JARS as 46.5 g $100\;g^{-1}$. The fatty acid composition of kernel was determined to those of oleic acid (31.3%) and linoleic acid (38.1%) from SD-F and LN-JARS. These results will be offered to useful information for breeding materials selection.

• Korean Journal of Environmental Agriculture
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• v.29 no.4
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• pp.408-416
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• 2010

To evaluate the effectiveness of AMF on the growth of horticultural crops, we compared mycorrhizal and non-mycorrhizal plants, perilla (P. frutescens Britt.), that were inoculated with AMF propagules. In the early stages of growth of perilla, compared to the AMF- perilla seedlings, in AMF+ perilla seedlings at 3 weeks after sowing, leaf length and width increased 17% and 29%, leaf area increased 28%, and shoot fresh weight increased 33%, root total length increased 1%, and chlorophyll content increased 3%. Further at 10 weeks after sowing, compared to the AMF- perilla plants, in perilla plants inoculated with AMF at the sowing and transplanting stages, leaf area increased 21% and 19%, shoot length increased 19% and 17%, root fresh weight increased 17% and 20%, and chlorophyll content increased 5.1% and 4.8%, respectively. Moreover, at 14 weeks after sowing, compared to the AMFperilla plants, in perilla plants inoculated with AMF at the sowing and transplanting stages, the number of leaves increased 16% and 20%, root fresh weight increased 16% and 17% significantly. Further, leaf fresh weight increased 9% and 11%, shoot diameter increased 4.5% and 7.3%, and chlorophyll content increased 1.5% and 2.5%, respectively. The levels of many macronutrients and micronutrients were tended to be significantly higher in AMF+ plants than in AMF- plants, supporting the association between AMF and enhanced growth of plants grown from AMF+ seedlings.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.379-387
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• 2010

Oilseed rape (Brassica napus L.) is an important crop due to its high oil content in the seed. Recently, the demand for the improvement of crop for biodisel energy source is increased as oil prices in the world has increased dramatically. Until now, oilseed rape breeding was carried out by cross-hybridization between different varieties and related germplasms. However, like as many other crops, the application of tissue culture and gene transformation systems has been introduced into oilseed rape breeding program including the development of transgenic canola plants. In this study, we reviewed a history of tissue culture and genetic transformation research in oilseed rape plants and indicated some important aspects for the production of transgenic oilseed rape plants.

• Journal of Plant Biotechnology
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• v.37 no.4
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• pp.456-464
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• 2010

Potato is one of the most important crops in the world. Due to vegetative propagation of this crop, techniques of plant tissue culture and genetic transformation are often applied for potato researches and a lot of progress has been made in the breeding programs using these techniques during the last decades. In potato, there have been several trials to introduce GM potato varieties to the world market, but they so far failed due to the changed legislation and unwillingness of large processors to process GM potatoes. These issues are highly associated with the general acceptances of the public and other political decisions. In addition to these, there are still obstacles to overcome to achieve the development of commercial potato variety and several factors to improve horticulturally important traits. In this study, therefore, we reviewed recent advances and research status on tissue culture and genetic transformation in potato and discussed future perspective.

• Journal of People, Plants, and Environment
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• v.24 no.6
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• pp.673-684
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• 2021

Background and objective: This study seeks to investigate domestic agro-healing farm resources so that preliminary data can be obtained on the systematic provisions for agro-healing programs in order to improve service quality and to identify the quality of agro-healing policy services. Methods: For this study, owners of agro-healing facilities were asked 24 questions via an online survey. An importance-performance analysis (IPA) was conducted to identify service quality for each policy sector. Results: Respondents' satisfaction levels were low when compared to agro-healing farm owners' perceptions of the above-average importance of each sector. The results from the resource analysis of domestic agro-healing farms indicated that the main operational purposes of farms were, in descending order, experience (90.0%), healing (86.7%), and education (84.0%). With respect to the type of program that was operated, cultivating crops (horticultural therapy) was identified most frequently at 83.3%. Conclusion: As a result of this study, the resources possessed by agro-healing farms were identified. In addition, the results of the IPA analysis in terms of service quality are not as good as expected, and performance levels should be increased to improve this. The results of this study are expected to provide useful information not only to improve the quality of agro-healing services, but also to revitalize the agro-healing industry while developing a systematic agro-healing program.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.233-233
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• 2022

As the world's population grows and food needs diversify, the demand for horticultural crops for beneficial traits is increasing. In order to meet this demand, it is necessary to develop suitable cultivars and breeding methods accordingly. Breeding methods have changed over time. With the recent development of sequencing technology, the concept of genomic selection (GS) has emerged as large-scale genome information can be used. GS shows good predictive ability even for quantitative traits by using various markers, breaking away from the limitations of Marker Assisted Selection (MAS). Moreover, GS using machine learning (ML) and deep learning (DL) has been studied recently. In this study, we aim to build a system that selects phenotype-related markers using the genomic information of the pepper population and trains a genomic selection model to select individuals from the validation population. We plan to establish an optimal genome wide association analysis model by comparing and analyzing five models. Validation of molecular markers by applying linkage markers discovered through genome wide association analysis to breeding populations. Finally, we plan to establish an optimal genome selection model by comparing and analyzing 12 genome selection models. Then We will use the genome selection model of the learning group in the breeding group to verify the prediction accuracy and discover a prediction model.

• Korean Journal of Agricultural Science
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• v.50 no.2
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• pp.235-248
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• 2023

Orchidaceae species account for one-tenth of all angiosperms including more than 30,000 species having significant ecological, evolutionary, and economic importance. Despite Orchidaceae being one of the largest families among flowering plants, crucial cytogenetic information for studying species diversification, inferring phylogenetic relationships, and designing efficient breeding strategies is lacking, except for 10% or less of orchid species cases involving mostly chromosome number or karyotype analysis. Also, only approximately 1.5% of the identified orchid species from less than a hundred genera have genome size data that provide crucial information for breeders and molecular geneticists. Various molecular cytogenetic techniques, such as fluorescence in situ hybridization (FISH) and genomic in situ hybridization (GISH), have been developed for determining ploidy levels, analyzing karyotypes, and evaluating hybridity, in several ornamental crops including orchids. The estimation of genome size and the determination of nuclear DNA content using flow cytometry have also been employed in some Orchidaceae subfamilies. These different techniques have played an important role in supplementing beneficial knowledge for effective plant breeding programs and other related plant research. This review focused on orchid breeding summarizes the status of current cytogenetic tools in terms of background, advancements, different techniques, significant findings, and research challenges. Principal roles and applications of cytogenetics in orchid breeding as well as different ploidy level determination methods crucial for breeding are also discussed.

• Horticultural Science & Technology
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• v.28 no.6
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• pp.1025-1038
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• 2010

Carrot ($Daucus$ $carota$ L. var. $sativa$) is one of the most widely used crops in the world. Moreover it is an important crop because of its high content of ${\beta}$-carotene, well-known as the precursor of vitamin A carotenoid. However, seed-hair which is generated in epidermal cell of seeds inhibits absorption and germination. For that reason, carrot seeds are commercialized after mechanical hair removal process. To overcome such cumbersome weaknesses, new breeding program for developing hairless-seed carrot cultivar has been needed. Therefore, in this study, cDNA libraries from seeds of short-hair seed phenotype CT-ATR615 OP 666-13line and hairy seed CT-ATR615 OP-CK1-9 line were constructed and expression patterns related to generation of seed-hair were analyzed by comparison of EST sequences. Differential EST sequence results between two lines were classified into FunCat functional categories based on the results of BlastX search. Higher expression quantities belonging to metabolic category were shown on short-hair seed line than hairy-seed one. Differential expression quantities between those two lines in the protein folding and stabilization, subcellular localization categories were supposed to contribute variously on the generation of seed-hair. We confirmed 50 and 59 SSR sites, and 2 SNP sites by analyzing EST sequences in two lines; thereafter, we designed SNP and SSR primer sets from these EST sequence information as a molecular marker. These markers are thought to be used in research of molecular markers for classification of carrot family and related to various traits, as well as seed-hair characteristic.

• Horticultural Science & Technology
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• v.32 no.2
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• pp.157-164
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• 2014

Objective of this research was to develop root media containing expanded rice hull (ERH) and carbonized rice hull (CRH). To achieve this, the physico chemical properties of two materials were analysed and blended with peatmoss (PM) or coir dust (CD) with various ratio. Based on the physical properties of the blended materials, 4 root media were selected for future experiment. After the analysis of pH and EC of the selected root media, the kinds and amount of pre-planting nutrient charge fertilizers (PNCF) incorporated into each root medium were varied, and then, final chemical properties of the root media were analysed. The total porosity (TP), container capacity (CC), and air-filled porosity (AFP) were 81.3%. 39.9%, and 41.4% in ERH and 77.6%, 64.1%, and 13.5% in CRH, respectively. The percentage of easily available water (EAW, from CC to 4.90 kPa tension) and buffering water (BW, 4.91-9.81 kPa tension) were 11.37% and 5.27%, in ERH and 17.26% and 14.28% in CRH, respectively. The pH of ERH was 7.1, but it was extremely high in CRH such as 11.2. The EC and CEC were $1.31dS{\cdot}m^{-1}$ and $12.1meq{\cdot}100g^{-1}$ in ERH and $6.53dS{\cdot}m^{-1}$ and CEC 7.79 $meq{\cdot}100g^{-1}$ in CRH, respectively. The ranges of TP, CC and AFP in 4 selected media (PM + ERH, 6:4, v/v; CD + ERH, 8:2; PM + CRH, 7:3; CD+CRH 6:4) were 89.2-90.3%, 67.3-81.8%, and 8.3-21.9%, respectively. The pHs and ECs in root media containing peatmoss such as PM + ERH (6:4) and PM + CRH (7:3) were 4.0-4.3 and $0.33-0.365dS{\cdot}m^{-1}$, whereas those of CD + CRH were 7.4-7.9 and $1.282dS{\cdot}m^{-1}$. The pHs and ECs, however, analysed before and after the incorporation of PNCF in each root medium were not significant different. This result indicated that the incorporated fertilizers in PNCF to adjust medium pH did not dissolve enough to influence medium pH, but it is very normal in root media containing dolomitic lime and sulfur powder in adjusting pH. The Information obtained in this study may facilitate an effective formulation of root media containing rice hulls.