• Journal of Information Display
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• v.7 no.1
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• pp.12-18
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• 2006

We investigated the horizontal alignment properties such as surface morphology, pretilt angle, and polar and azimuthal anchoring energy of organic alignment material-coated surface treated by ion beam. In this investigation the energy and incident angle of ion beam were changed. We also fabricated an in-plane switching (IPS) cell by ion beam alignment. The results showed similar voltage-transmittance characteristics to those of a rubbed cell and better dark state.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2003.07b
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• pp.957-960
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• 2003

We have studied the influence of rubbing directions on electro-optic characteristics of the fringe field driven hybrid aligned nematic liquid crystal (LC) cell by computer simulation. When a LC with positive dielectric anisotropy is used, the results show that the driving voltage and transmittance decreases as the rubbing direction with respect to horizontal electric field direction increases. The reason for decrease in light transmission is the reduction of twist angle and increase of tilt angle on the center of common electrode. In this paper, we have studied how the rubbing angle effects on the transmittance of the cell by investigating a distribution of electric field and LC director.

• Proceedings of the KSME Conference
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• 2008.11a
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• pp.1404-1408
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• 2008

The migration and proliferation of vascular endothelial cells (VEC), which play an important role in vascular remodeling, are known to be regulated by hemodynamic forces in the blood vessels. When shear stresses of 2, 6, 15 dynes/$cm^2$ are applied on mouse micro-VEC in vitro, cells surprisingly migrate against the flow direction at all conditions. While higher flow rate imposes more resistance against the cells, reducing their migration speed, the horizontal component of the velocity parallel to the flow increases with the flow rate, indicating the higher alignment of cells in the direction parallel to the flow at a higher shear stress. In addition, cells exhibit substrate stiffness and calcium dependent migration behavior, which can be explained by polarized remodeling in the mechanosensitive pathway under shear stress.

• 한국정보디스플레이학회:학술대회논문집
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• 2009.10a
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• pp.502-504
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• 2009

We demonstrate a pixel-isolated bistable twist-splay namatic (PI-BTSN) liquid crystal (LC) cell which has two stable states of splay and ${\pi}$-twist. Each state is stabilized by a multi-dimensional anchoring effect of pixel-isolating polymer walls without any chiral additives. Polymer walls are formed around the pixel region by anisotropic phase separation between LCs and reactive mesogens. Switching between the two states is archived by using vertical and horizontal electric fields. The memory mode of the fabricated LC cell has shown infinity memory time.

• Journal of Information Display
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• v.4 no.2
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• pp.25-28
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• 2003

We propose a new optical configuration of a hybrid-aligned nematic liquid crystal cell for reflective displays, which consist of a biaxial film for obtaining wide viewing angle and a half-wave film for achieving high contrast. As a result, we can achieve wide viewing angle of (80( for the horizontal direction and (50( for the vertical direction and uniform reflection spectrum.

• Transactions of the Korean Society of Mechanical Engineers
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• v.14 no.3
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• pp.702-715
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• 1990

This paper describes behaviors of two-phase open channel flow inside the flash chamber of a horizontal Multi-Stage-Flash evaporator numerically along with the experimental observations. Bubble trajectories and the velocity and temperature distributions of the liquid phase were predicted by using the particle-source-in-cell(PSI-Cell) method with the appropriate bubble motion/growth equations. Size and number of bubble nuclei embedded in the incoming liquid(brine) were taken into account as important parameters in addition to the conventional ones such as the velocity, degree of inlet superheat, inlet opening height, and the liquid level. Bubble motions, which are unsteady, appeared to be mostly determined by the buoyancy and the drag forces. The calculations, though a number of simplifying assumptions were made, reasonably simulated the hydrodynamic behaviors of the two-phase horizontal stream observed in the experiments. The simulated temperature distributions also agreed fairly well with the other's measurements. Non-equilibrium allownaces, evaluated from the simulated temperature distributions, were within the range of those obtained from the existing correlations, and reduced with the increases of the number and size of incoming bubble nuclei due to vigorous flashing.

• International Journal of Stem Cells
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• v.16 no.4
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• pp.438-447
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• 2023

Recently, ex-vivo gene therapy has emerged as a promising approach to enhance the therapeutic potential of mesenchymal stem cells (MSCs) by introducing functional genes in vitro. Here, we explored the need of using selection markers to increase the gene delivery efficiency and evaluated the potential risks associated with their use in the manufacturing process. We used MSCs/CD that carry the cytosine deaminase gene (CD) as a therapeutic gene and a puromycin resistance gene (PuroR) as a selection marker. We evaluated the correlation between the therapeutic efficacy and the purity of therapeutic MSCs/CD by examining their anti-cancer effect on co-cultured U87/GFP cells. To simulate in vivo horizontal transfer of the PuroR gene in vivo, we generated a puromycin-resistant E. coli (E. coli/PuroR) by introducing the PuroR gene and assessed its responsiveness to various antibiotics. We found that the anti-cancer effect of MSCs/CD was directly proportional to their purity, suggesting the crucial role of the PuroR gene in eliminating impure unmodified MSCs and enhancing the purity of MSCs/CD during the manufacturing process. Additionally, we found that clinically available antibiotics were effective in inhibiting the growth of hypothetical microorganism, E. coli/PuroR. In summary, our study highlights the potential benefits of using the PuroR gene as a selection marker to enhance the purity and efficacy of therapeutic cells in MSC-based gene therapy. Furthermore, our study suggests that the potential risk of horizontal transfer of antibiotics resistance genes in vivo can be effectively managed by clinically available antibiotics.

• Transactions of the Korean hydrogen and new energy society
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• v.19 no.4
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• pp.283-290
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• 2008

In this paper, experiments with an air-breathing proton exchange membrane fuel cell (PEMFC) for mobile devices were carried out according to cathode conditions. These conditions are defined by the cathode flow field plate type (the channel type, the open type) and the cathode surface direction. Single-cell and 6-cell stack were used in the experiments. The experimental results showed that the open-type cathode flow field plate gave a better performance than the small channel type. In the experiments related to the direction of the slits on the cathode flow field plate, the horizontal slit cell was better than the vertical one. With respect to the cathode surface direction, when the cathode surface is placed in the direction normal to the ground, the PEMFC generated more stable power in the mass transport loss region. Since stable power in the mass transport region is closely related to the air supply, computational fluid dynamics (CFD) analysis for air-breathing PEMFC of different cathode surface directions was performed.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.17 no.2
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• pp.201-206
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• 2004

We have performed computer simulation and experiment to obtain electro-optic characteristics of reflective hybrid aligned nematic (R-HAN) cell driven by fringe field, in which the cell consists of polarizer, optical compensation film, LC layer and reflector. Conventional R-HAN cell driven by fringe field using only the LC layer shows high wavelength dispersion at dark-state and thus viewing angle characteristic is strongly wavelength-dependent. In order to improve this demerit, we added one optical compensation film to conventional R-HAN cell. The display with optimized cell parameters shows low wavelength dispersion at dark-state and exhibits a wide viewing angle without the occurrence of grey scale inversion over a wide range of viewing angles and the contrast ratio greater than 5 over exists about 120$^{\circ}$ in vortical direction and 160$^{\circ}$in horizontal direction. Experimental results show good agreements with theoretical results and fast response time.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.38 no.1
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• pp.63-71
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• 2018

In this study, the torque and horizontal stress acting on vane were measured and then used to determine a friction angle of sand. A dry Nakdong River sand was prepared for loose and dense conditions in a cell and then pressurized with 25, 50, 75 or 100 kPa from the surface of sand. A vane (5cm in diameter and 10cm in height) was rotated and the torque and horizontal stress were measured at real time. A maximum torque was 3.5-9.5Nm for loose sand and 7.4-17.6Nm for dense sand, respectively. The maximum torque increased as an overburden pressure increased. The maximum torque obtained at 14-20 degrees of vane rotation, which was not influenced by the initial alignment of earth pressure and vane blade. An initial horizontal stress ratio was 0.33-0.35 on the average. The horizontal stress increased initially and then decreased due to particle disturbance. A friction angle was calculated from real time varying horizontal stress and torque, which decreased with increasing overburden pressure. The friction angle of loose sand from vane shear test was similar to that of direct shear test but that of dense sand was overestimated.