• Title/Summary/Keyword: Hook-effect

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Effect of Benzyladenopurine Treatment Time during Imbibition on Growth of Soybean Sprouts and its Cost (BA 처리시기에 따른 콩나물의 생장과 처리비용 분석)

  • 강진호;전병삼;이상우;정종일;심상인
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.3
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    • pp.232-237
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    • 2003
  • Benzyladenopuyine (BA) commonly used for soybean sprout production is of high price. The study was carried out to determine the effect of BA treatment time during seed imbibition on growth and development of soybean sprouts and to analyse its treatment cost. The soybean seeds of 4 cultivars were soaked in 4 ppm BA solution during the first 5.5 hour imbibition (EFHI), the second 5.5 hour imbibition (SFHI) immediately after 0.5 hour aeration, or whole 11.0 hour imbibition (WFHI) intervened by aeration. On the 6th day after culture, the soybean sprouts were classified into 4 categories on the base of hypocotyl length; > 7cm, 4 to 7cm, < 4 cm and not germinated, and their morphological characters, fresh and dry weights were measured. Water absorption of the seeds was sharply reduced after it was almost done for the first 5.5 hours. The percentage of sprouts with hypocotyls of longer than 4cm was higher in FFHI treatment than in the other two ones. Regardless of BA imbibition time and periods, the lateral roots were not observed. WFHI treatment showed shorter hypocotyl and root lengths but thicker hypocotyl and hook diameters than FFHI and SFHI treatments. All component fresh and dry weights except cotyledon fresh weight were nearly same. Treatment cost of BA was the lowest in SFHI treatment. It is concluded that BA treatment during SFHI is the best time because its treatment time did not affect sprout growth but its treatment cost.

Development of Enzyme Immuno Assay for Analysis of Free Prostate Specific Antigen in Serum (혈청 유리형 전립선항원 (free PSA) 측정을 위한 효소면역측정법의 개발)

  • Kyung-Ok Lee;Kyung-In Kim;Kyu-Pum Lee
    • Biomedical Science Letters
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    • v.3 no.2
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    • pp.107-114
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    • 1997
  • Recent reports indicate that the clinical usefulness of prostate specific antigen (PSA), particulary in the differentiation of benign prostate hyperplasia from prostate cancer, can be improved by measuring the amount of free PSA in serum. Measuring free PSA is especially useful in attempts to improve diagnositc performance of PSA in the diagnostic gray zone of total PSA. The objective of this study was to develop free PSA assay kit using sandwich microplate enzyme immunoassay format. We chose a test format with polyclonal anti-PSA antibodies coated on the wells and monoclonal anti-free PSA antibodies for quantification to gain higher test sensitivity. We adpoted 10 uL of specimen and 2 hours of first incubation time with detecting antibody for free PSA EIA format using microplate. The within-day and between-day precision (%CV) in the high and low concentration ranges were below 4%. The correlation coefficient between in-house free PSA assay and commercial assay kit was r=0.9965 (slope=0.0984, y intercept=0.0173, N=27). No hook effect was found by 40 ng/mL and correlation coefficient (r) value of the fitted linear regression was over 0.995. The recovery tests were in the range of 98.9∼104.1% for free PSA. In conclusion, in-house free PSA enzyme immune assay is cost effective, simple and rapid and could be useful for the prognosis after theraphy as well as for the differential diagnosis between prostate cancer and benign prostate hyperplasia.

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Effect of Benzyladenopurine Concentration on Growth and Morphology of Soybean Sprouts and Comparison with Selling Products (BA 처리농도에 따른 콩나물의 생장과 시판 콩나물과의 비교)

  • 강진호;조용준;전병삼;윤수영;전승호;김희규
    • Korean Journal of Plant Resources
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    • v.17 no.2
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    • pp.94-101
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    • 2004
  • Commercial value of soybean sprouts should be affected by their morphology including the lateral roots. This study was carried out to determine the effect of benzyladenopurine (BA) concentration on growth and morphology of soybean sprouts in order to compare them with the selling products collected from the markets. Four cultivars (cv. Eunhakong, Pungsannamulkong, Sowonkong, and Junjery) were cultured for 6 days after imbibed for 5 hours into different BA solutions (0, 1,2,4, and 8 ppm). On the 6th day, hypocotyl length was measured to calculate the composition rate of > 7 cm (A), 4 to 7 cm (B), < 4 cm (C) and non-germination (D), and the ratio of hypocotyl to root length (H/R ratio) on the base of hypocotyl length as well as lateral root, hypocotyl diameter, and fraction fresh and dry weights. The composition rates classified with hypocotyl length showed big differences between 4 cultivars in class A and C; Class A, in which cv. Sowonkong and Junjery had higher rates than cv. Eunhakong and Pungsannamulkong, was decreased with BA concentration of higher than 4 ppm, but class C showed the reverse result to class A. Any lateral root was not formed in higher than 4 ppm BA solutions although fewer in cv. Pungsannamulkong and Junjery than in the two other cultivars. Hypocotyl and root lengths were decreased with increased BA concentrations, and in all BA concentration, hypocotyl length of cv. Junjery was the longest. Hypocotyl diameter, hypocotyl and total fresh weights were thicker and heavier in 2 to 4 ppm BA concentrations than in the other ones, although in case of BA application, cv, Eunhakong and Sowonkong were heavier. The H/R ratios were increased with increased BA concentration. The ratios of nearly all the selling products ranged from 1 to 2 ppm when compared to them from our experiment.

Effect of Circumferential Tool Path Control on Friction Stir Spot Welding of Al/Fe Dissimilar Metal Joint (툴 경로제어를 이용한 Al/Fe 이종금속 마찰교반점용접 공정특성 평가)

  • Yoon, Jin Young;Kim, Cheolhee;Rhee, Sehun
    • Journal of Welding and Joining
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    • v.34 no.3
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    • pp.6-11
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    • 2016
  • Joining Al/Fe dissimilar metals is becoming a subject of special interest in the assembly of automotive parts as a trade-off between the weight lightening and the cost reduction. Although various studies have been introduced to join Al alloy with the steel sheet by fusion welding, weak joint strength and galvanic corrosion still remained as problems to be solved. As a solid state welding, friction stir welding has been preferred to fusion welding processes in the dissimilar metal joints. This study investigated friction stir spot welding (FSSW) of Al alloy to the thin steel sheet with a thickness of 0.65 mm. The conventional FSSW is a stationary spot welding process but new approach adopted an additional circumferential movement in company with high speed tool rotation. A full factorial experimental design was implemented, and the main and interaction effects of parameters were analysed on the failure load in the tensile shear test. The direction and radius of rotation were statistically significant parameters and these two parameters affected the joint width and the shape of the hook.

Effect of Volume Fraction and Length of Fiber on the Mechanical Properties of Fiber Reinforced Concrete (섬유보강 콘크리트의 역학적 특성에 대한 섬유 체적비와 길이의 영향)

  • Yang, Keun-Hyeok;O, Seung-Jin
    • Journal of the Korea Institute of Building Construction
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    • v.8 no.1
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    • pp.43-48
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    • 2008
  • Fifteen concrete specimens were mixed and tested to explore the significance and limitation of appling the polyvinyl alcohol (PVA) fiber and steel fiber with end hook to concrete. Main parameters investigated were volume fraction and length of the fibers. The measured mechanical properties of fiber reinforced concrete are analyzed according to the equivalent fiber amount index explaining the adding amount and length of fibers. Test results showed that compressive strength of fiber reinforced concrete was higher than that of concrete with no fiber by $10{\sim}20%$. The normalized splitting tensile strength and flexural strength of PVA fiber reinforced concrete were similar to those of concrete with no fiber, whereas those of steel fiber reinforced concrete increased with the increase of the equivalent fiber amount index. In particular, much higher ductile behavior was observed in steel fiber reinforced concrete than in PVA reinforced concrete, indicating that the slope of descending branch of load-displacement relationship of steel fiber reinforced concrete decreased with the increase of the volume fraction and length of the fiber.

The suture bridge transosseous equivalent technique for Bony Bankart lesion

  • Choe, Chang-Hyeok;Kim, Sin-Geun;Baek, Seung-Hun;Sin, Dong-Yeong
    • The Academic Congress of Korean Shoulder and Elbow Society
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    • 2008.03a
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    • pp.178-178
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    • 2008
  • In order to improve static stability and healing of reattached labrum, we combined the advantages of suture bridge and transosseous technique. Using the conventional 3 portal for anterior instability, check stability of bony Bankart and preparation of glenoid bed in 3 way including removal, reshaping or mobilization of bony fragment. Two anchors were inserted to the superior and inferior portion and medial edge of bony Bankart lesion. It usually corresponded to the area of IGHL. Medial mattress sutures were applied around IGHL complex to get enough depth of glenoid coverage using suture hook. Make 3.5mm pushlock anchor hole to the articular edge of glenoid cartilage. Proximal suture bridge was applied at first and then distal suture bridge was inserted to mobilize the labrum in proximal direction. These construction can provide more stable labral repair with wide contact and compression in case of deficient bony stability. It not only avoids technical disadvantage of point contact with anchor fixation, but also decreasing gap formation through cross compression of labrum that couldn't gain even with the transosseous fixation which affords linear compression effect. Additional bony stability could be gained if the the bony fragment was mobilized to the glenoid margin with potential healing bed or reshaped for the good contact with reattached labrum.

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Anti-dementia Effects of Gouteng-san and Si-Wu-Tang

  • Watanabe, Hiroshi
    • Toxicological Research
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    • v.17
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    • pp.257-261
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    • 2001
  • Recently, a traditional medicine called Gouteng-san, which consists of eleven herbs, was reported to be effective in treating vascular dementia with a double-blind, placebo-controlled study. Gout-eng-san is also used for patients with vascular dementia in combination with Si-Wu-Tang. The effect of Gouteng-san and Si-Wu-Tang on deficit of learning behavior was investigated using step-down passive avoidance task in mice. Hot-water extract of Gouteng-san (1.5 and 6 g/kg, p.o.) significantly prolonged the step-down latency shortened by scopolamine. The extract of Uncaria hook (150 mg/kg, p.o.), one of the component herb of Gouteng-san, significantly prevented the decrease in the latency after scopolamine. Hot-water extract of Si-Wu-Tang (1.5 and 6 g/kg of dried herbs, p.o.) prevented dose-dependently scopola-mine-induced disruption qf learning behavior. Si-Wu-Tang also prevented the ischemia-induced deficit of learning behavior. Both hot water extract of peony and angelica (1.5 g/kg, p.o.), which are component herbs qf Si-Wu-Tang, prevented the scopolamine-induced learning behavior deficit. Scopolamine (10 uM) suppressed long-term potentiation (LTP) of population spike in the CA1 region of the rat hippocampal slices. Peoniflorin (0.1~ 1uM) extracted from paeony root significantly ameliorated scopolamine-induced inhibition of LTR These results suggest that improvement of deficit of learning behavior by Gouteng-san and Si-Wu-Tang is mediated by direct and/or indirect activation of the cholinergic system in the brain.

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Enhanced Anti-tumor Efficacy of Aspirin Combined with Triptolide in Cervical Cancer Cells

  • Chen, Rong-Hui;Tian, Yong-Jie
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.5
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    • pp.3041-3044
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    • 2013
  • Background: The non-steroidal anti-inflammatory drug (NSAID) aspirin (acetylsalicylic acid) is an inhibitor of cyclooxygenase enzymes. Recent studies have shown that aspirin could be used as an anti-tumor drug. Triptolide, the major compound extracted from the Chinese herb Tripteryglum wilfordii Hook.f, has now been shown that it can inhibit tumor growth. The aim of this study was to analyze the anti-tumor efficiency of aspirin and triptolide in cervical cancer cells. Methods: Viability of cervical cancer cell lines was assessed by the MTT method at various concentrations of aspirin and triptolide. Siha and HeLa cell apoptotic analysis was performed by flow cytometry. Real time-PCR and Western Blotting were used to analyze the expression of Bcl-2/Bax, Cyclin D1 and p16. Results: Viability in the combination group was significantly decreased as compared with either drug used alone. Expression change of Bcl-2/Bax, CyclinD1 and p16 appeared to play an important role in the synergistic killing effect on cervical cancer cell apoptosis. Conclusion: Aspirin and triptolide combination treatment may have synergistic anti-tumor effects on cervical cancer cells.

Pull-Out Behaviour of Hooked End Steel Fibres Embedded in Ultra-high Performance Mortar with Various W/B Ratios

  • Abdallah, Sadoon;Fan, Mizi;Zhou, Xiangming
    • International Journal of Concrete Structures and Materials
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    • v.11 no.2
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    • pp.301-313
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    • 2017
  • This paper presents the fibre-matrix interfacial properties of hooked end steel fibres embedded in ultra-high performance mortars with various water/binder (W/B) ratios. The principle objective was to improve bond behaviour in terms of bond strength by reducing the (W/B) ratio to a minimum. Results show that a decrease in W/B ratio has a significant effect on the bond-slip behaviour of both types of 3D fibres, especially when the W/B ratio was reduced from 0.25 to 0.15. Furthermore, the optimization in maximizing pullout load and total pullout work is found to be more prominent for the 3D fibres with a larger diameter than for fibres with a smaller diameter. On the contrary, increasing the embedded length of the 3D fibres did not result in an improvement on the maximum pullout load, but increase in the total pullout work.

$\Delta$-plan and spin in the golf swing (골프 스윙에서 $\Delta$-평면과 스핀)

  • Jo, Chang-Ho;Park, Jong-Dae;Lee, Kun-Chun
    • The Journal of Natural Sciences
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    • v.14 no.2
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    • pp.1-14
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    • 2004
  • The observation of the various swing parameters and its effect on golfer swing are tatally analyzed. The golf ball impact and flies away, therefore it must be controlled bofore ball impact. The purpose of this paper is that the cause of hook and slice are found and the posture of swing is corrected. The trajectory after ball impact is on the $\Delta$-plane, which is consisted of the normal vector on club face and the swing velocity vector of club head including the initial celocity and the spin axis after ball imfact. In order to correct the miss shot in golf swing, this paper is is shown that the theoretical review and study is discussed to use the D-plane and $\Delta$-plane.

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