• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.7
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• pp.3103-3108
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• 2011

This work was aimed to evaluate supplementation of honey as sole carbon source on the fermentation characteristics of wine fermented with fruit of flowering cherry and honey(flowering cherry-honey wine). Physiochemical changes of flowering cherry-honey wine(2 L) were investigated during 30 days in fermentation by strain of Saccharomyces bayanus (EC-118). At the beginning of fermentation, fructose was most abundant sugar then glucose and sucrose were followed. As fermentation proceeded, utilization of glucose by S. bayanus (EC-118) was faster than fructose, so that the ratio of fructose/glucose was increased. During fermentation for 30 days, pH and viable yeast count was changed rapidly between 0 to 5 days, while $^{\circ}Brix$(%) decreased gradually for 30 days. Final total titratable acidity, pH, $^{\circ}Brix$(%) and ethanol content of flowering cherry-honey wine were 0.43%, pH 3.5, $9.7^{\circ}Brix$(%) and 14%, respectively. Our finding demonstrate that flowering cherry-honey could be benefical supplements for wine production.

• Korean Journal of Plant Resources
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• v.31 no.6
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• pp.612-621
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• 2018

Honey used as conventional medicine has various pharmacological properties. In the honey and anti-inflammatory effect, Gelam honey and Manuka honey has been reported to exert anti-inflammatory activity. However, the anti-inflammatory effect and potential mechanisms of acacia honey (AH) are not well understood. In this study, we investigated anti-inflammatory activity and mechanism of action of AH in LPS-stimulated RAW264.7 cells. AH attenuated NO production through inhibition of iNOS expression in LPS-stimulated RAW264.7 cells. AH also decreased the expressions of $IL-1{\beta}$, IL-6 and $TNF-{\alpha}$ as pro-inflammatory cytokines, and MCP-1 expression as a pro-inflammatory chemokine. In the elucidation of the molecular mechanisms, AH decreased LPS-mediated $I{\kappa}B$-${\alpha}$ degradation and subsequent nuclear accumulation of p65, which resulted in the inhibition of $NF-{\kappa}B$ activation in RAW264.7 cells. AH dose-dependently suppressed LPS-mediated phosphorylation of ERK1/2 and p38 in RAW264.7 cells. In addition, AH significantly inhibited ATF2 phosphorylation and nuclear accumulation of ATF2 in LPS-stimulated RAW264.7 cells. These results suggest that AH has an anti-inflammatory effect, inhibiting the production of pro-inflammatory mediators such as NO, iNOS, $TNF-{\alpha}$, IL-6, $IL-1{\beta}$ and MCP-1 via interruption of the $NF-{\kappa}B$ and MAPK/ATF2 signaling pathways.

• Journal of Apiculture
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• v.34 no.4
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• pp.315-323
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• 2019

Large amounts of sludge produced by paper mill industries represent one of the most serious environmental problems in the world. Recently, beekeepers living in the neighborhood of the paper mill in Hwasan County, Youngcheon city, GB, Korea, became alarmed that honey bee colonies were dying off suddenly across the neighborhood. A preliminary study was conducted to evaluate the toxicity (oral, fumigation, repellent) of recycled solid paper mill sludge (SPMS) and leachate paper mill sludge (LPMS) to honey bee workers under laboratory conditions, and to analyze the volatile organic compounds(VOC). The SPMS and LPMS were separately subjected to a liquid-liquid extraction (LLE) at three temperatures to extract VOC(highest VOC yields: 1.52% SPMS and 0.34% LPMS). A total of 70 chemicals were detected in the VOC of paper mill sludges, of which 49 and 21 volatile organic compounds from SPMS and LPMS, respectively. The SPMS was dominated by high degree presence of stanols (saturated sterols), such as cholestanol, cholestan-3-ol and also saturated hydrocarbons. However, LPMS was characterized by the absence of sterols. Both SPMS and LPMS showed an influence on the olfactory behavior of honey bee on Y-tube assay, with repulsion rates of 72 and 68%, respectively. Both SPMS and LPMS at concentration of 100mg/mL caused higher honey bee oral mortality than the untreated controls at 48, 72, 96 and 120 hours after treatment(highest oral mortality at 120 hr: 85.74%(SPMS); 93.51 % (LPMS)). A similar pattern was observed when honey bees were tested to fumigant toxicity. Both SPMS and LPMS caused significant higher mortality than the untreated control 24 hour after the exposure (highest fumigation mortality at 120 hr: 69.4% (SPMS); 56.8% (LPMS)). These preliminary results indicated that paper mill sludge could be partly responsible for sudden death and disappearance of honey bees, especially in hot humid summer days. With climate change, the risk of environmental chemical exposure to honey bee would pose greater attention.

• Korean Journal of Pharmacognosy
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• v.53 no.2
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• pp.111-118
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• 2022

Chestnut honey is a sweet dark-colored honey with a distinct bitter aftertaste. It contains numerous phenolic compounds and alkaloids and is noted for its antioxidant and anti-inflammatory activities. However, it has been established that there are differences in the composition and activity of chestnut honey constituents depending on the region of origin, the sources of which warrant further research. In this study, we analyzed the kynurenic acid (KA) contents in chestnut honey produced in nine different regions in Korea, using high-performance liquid chromatography in conjunction with ultraviolet detection, and validated the analytical method developed. Use of a reverse-phase column and detection at a wavelength of 240 nm were found to be optimal for the detection of KA. Similar evaluation of an optimal method for extracting KA from chestnut honey revealed that extraction using 10% EtOH at 20 times the sample volume over a 6 h period was the most suitable for obtaining a high content of KA. Among the nine regional chestnut honeys assessed, KA content was found to be highest in the "Gongju" sample (1.14 mg/g), followed by that in the "Cheongdo" and "Damyang" samples. Validation of the KA analytical method revealed a good analyte linearity, with a correlation coefficient (r²) of 0.9995, an accuracy of between 92.37% and 107.35%, and good precision (RSD ≤ 1.05%). Our findings in this study, based on a validated quantitative analytical method for KA, could make an important contribution to establishing a data profiling procedure for characterizing chestnut honeys produced in different regions, and may also provide basic data for the identification of functional honey.

• Korean Journal of Food Science and Technology
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• v.40 no.1
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• pp.8-14
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• 2008

This study was performed to establish analysis methods, and evaluated for grayanotoxin in domestic/foreign honey and wild honey. The molecular weight of grayanotoxins I, II and III, excluding grayanotoxin III that has been commercialized, were analyzed by LC-MS/MS. Then, the molecular structure of grayanotoxins I and II were analyzed by NMR. A total 111 samples (25 Korean honey, 21 Korean wild honey, 13 Korean honeycomb honey, 44 foreign honey, 8 foreign wild honey) were examined to determined whether or not each sample contained grayanotoxins I, II, and III. The honey samples were mixed with methanol and loaded into a tC18 cartridge, the filtrate was diluted with water, and the mixture was then analyzed by ESI triple-quadrupole LC-MS/MS. Grayanotoxins were only found in the foreign wild honey and were not detected in Korean honey, Korean honeycomb honey, or Korean wild honey. Three of the samples contained grayanotoxin I, II, and III, and one sample contained only grayanotoxins I and III. The lowest level for grayanotoxin I was 3.13 ${\pm}$ 0.00 mg/kg, and the highest level was 12.93 ${\pm}$ 0.01 mg/kg. The levels of grayanotoxin II were 0.84 ${\pm}$ 0.01 mg/kg, 0.92 ${\pm}$ 0.00 mg/kg and 1.08 ${\pm}$ 0.01 mg/kg, respectively. The lowest level of grayanotoxin III was 0.25 ${\pm}$ 0.01 mg/kg and the highest level was 3.29 ${\pm}$ 0.74 mg/kg. Through this study, safety management for foreign wild honey has been enabled.

• Journal of The Korean Society of Clinical Toxicology
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• v.4 no.2
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• pp.166-169
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• 2006

Poisonings caused by 'mad honey' are known to occur in response to grayanotoxins, which bind to sodium channels in the cell membrane, increasing membrane sodium permeability and preventing inactivation. Mild symptoms of mad honey intoxication are dizziness, weakness, hypersalivation, nausea, vomiting, and paresthesia. Severe intoxication, however, leads to serious cardiac manifestations such as atrioventricular block, dose-dependent hypotension, bradycardia, and respiratory depression. Atropine and vasoactive drugs improve symptoms of both bradycardia and respiratory rate depression. We report an unusual case of saliva-type hyperamylasemia in a mad honey poisoning patient who developed clinically significant bradycardia. She recovered fully within 3 days following atropine administration and medical treatment.

• Korean Journal of Agricultural Science
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• v.37 no.1
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• pp.143-149
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• 2010

This study analyzed the income of large scale honey bee farming of Korea. Using a questionnaire, 44 large scale honey bee farming farmhouseholds were surveyed. The average hive sizes are 258. The results are as follows. First, gross receipts of bee farming farmhouseholds are 50,342 thousand won, and it's components are honey 71%, bee selling and/or lent 26%. Second, working expenses are 23,778 thousand won that is 47% of gross receipts. Feed costs are 38.2%, material costs are 23.9%, hired labor costs are 11.0%, moving costs are 10.1%. Finally, receipts per hive are 195.1 thousand won, working expenses per hive are 23,778 thousand won, so income per hive is 103.0 thousand won.

• Journal of Electrical Engineering and Technology
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• v.13 no.2
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• pp.998-1007
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• 2018

The advent of Wireless Sensor Networks (WSN) has led to their use in numerous applications. Sensors are autonomous in nature and are constrained by limited resources. Designing an autonomous topology with criteria for economic and energy conservation is considered a major goal in WSN. The proposed honey-hive clustering consumes minimum energy and resources with minimal transmission delay compared to the existing approaches. The honey-hive approach consists of two phases. The first phase is an Intra-Cluster Min-Max Discrepancy (ICMMD) analysis, which is based on the local honey-hive data gathering technique and the second phase is Inter-Cluster Frequency Matching (ICFM), which is based on the global optimal data aggregation. The proposed data aggregation mechanism increases the optimal connectivity range of the sensor node to a considerable degree for inter-cluster and intra-cluster coverage with an improved optimal energy conservation.

• Korean Journal of Veterinary Service
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• v.19 no.2
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• pp.172-179
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• 1996

This study was to investigate the properties such as amounts of moisture, inverted sugar, cane sugar and 5-(hydroxymethyl)-2-furfural (HMF) in feeding-honey(n=45) and wild-honey (n=3) harvested in Chonbuk area from April to November 1995. The results obtained were summarized as follows ; The average percentages of moisture in both feeding- and wild-honey was 20.2%(range, 17.5~23.1) and 20.7%(19.4~22.2). And those of inverted sugar/cane sugar in both honeies were 64.4%(55.8~69.3)/6.8%(1.0~l5.7) and 68.9%(68.5~69.8)/3.1%(2.5~3.7), in seasonal analysis those were 65.9%/5.3% in spring, 65.7%/6.6% in summer, 62.1%/8.0% in autumn, respectively. On the other hand, the average amounts of HMF in both honeies were 44.86mg/kg(22.6~68.6) and 7.0mg/kg(6.2~7.6), its average difference between both honeies were about 37.86mg/kg regardless of region and season.

• YAKHAK HOEJI
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• v.21 no.2
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• pp.87-94
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• 1977

The rheological properties of some honey-ginseng powder mixturs, used as pharmaceutical pills, were studied extensively by employing five kinds of honey, acacia, chestnut, buckwheat, green perilla and bush clover. Relation between the viscosity of the sample mixtures and the concentrations of ginseng powder and the temperature effect on the internal change of the structure were studied. The results obtained are as follows: 1) A complete gel formation of honey-ginseng mixtures was observed when about 50 days have passed after the sample preparation. 2) The viscosity of honey-ginseng powder mixtures was related with the concentration of ginseng powder, the temperature and the period of time required in gel formation as shown in the following equation. n=noexp$[{\kappa}_{1}c^{n1}+{\kappa}_{2}(T-273)^{-n2}+{\kappa}_{3} t^{n3}]$