• 한국응용곤충학회지
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• 제48권3호
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• pp.393-401
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• 2009

토마토 및 경제작물의 수분용으로 50,000봉군 정도 이용되고 있는 서양뒤영벌을 시설완숙 토마토하우스에 방사하여 품종별로 효율적인 사용과 경제적 효과를 알아보기 위하여 2008년 2월부터 8월까지 춘천시 에서 추가화분공급효과를 조사하였다. 슈퍼도태랑, 제우스42, 그리고 슈퍼선로드 품종에서 서양뒤영벌 봉군내 추가화분공급($5{\sim}6g$/3일)으로 화분매개활동과 방화흔 비율 그리고 봉군수명이 뚜렷하게 나타났다. 특히 슈퍼도태랑과 슈퍼선로드품종에서 추가화분공급효과가 더 크게 나타났다.

• The Korean Journal of Physiology and Pharmacology
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• 제22권1호
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• pp.101-110
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• 2018

In this study, we aimed to investigate the neuroprotective effects of caffeic acid phenethyl ester (CAPE), an active component of propolis purified from honeybee hives, on photothrombotic cortical ischemic injury in mice. Permanent focal ischemia was achieved in the medial frontal and somatosensory cortices of anesthetized male C57BL/6 mice by irradiation of the skull with cold light laser in combination with systemic administration of rose bengal. The animals were treated with CAPE (0.5-5 mg/kg, i.p.) twice 1 and 6 h after ischemic insult. CAPE significantly reduced the infarct size as well as the expression of tumor necrosis $factor-{\alpha}$, hypoxiainducible $factor-1{\alpha}$ monocyte chemoattractant protein-1, $interleukin-1{\alpha}$, and indoleamine 2,3-dioxygenase in the cerebral cortex ipsilateral to the photothrombosis. Moreover, it induced an increase in heme oxygenase-1 immunoreactivity and interleukin-10 expression. These results suggest that CAPE exerts a remarkable neuroprotective effect on ischemic brain injury via its anti-inflammatory properties, thereby providing a benefit to the therapy of cerebral infarction.

• 한국비블리아학회지
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• 제26권1호
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• pp.391-428
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• 2015

이 연구는 국가기록원이 다양해진 이용자들의 요구를 충족시키기 위한 기록정보서비스 방법 중 하나로 아카이브 이벤트의 활성화 방안을 모색하는데 있다. 이를 위해 미국의 독자적 기록물관리기관인 국립기록관리처(NARA)와 영국의 국립보존기록관(TNA)에서 운영되는 아카이브 이벤트 사례와 국가기록원 아카이브 이벤트를 비교분석하였다. 이를 바탕으로 활성화 방안으로 주제 영역의 세분화, 프로그램 유형의 다양화, 아카이브 이벤트의 연계성 강화를 제시하였다.

• 농촌의학ㆍ지역보건
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• 제44권3호
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• pp.138-143
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• 2019

양봉 작업은 들기 작업이 많아 근골격계 부담이 컸으며 양봉 농업인의 대부분이 지난 1년 동안 직업과 관련된 근골격계 질환 증상을 호소했다. 보조도구인 벌통리프트를 사용하면 양봉 작업으로 인한 근골격계 부담을 줄일 수 있다.

• Journal of Microbiology and Biotechnology
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• 제33권11호
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• pp.1495-1505
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• 2023

The western honeybee Apis mellifera L., a vital crop pollinator and producer of honey and royal jelly, faces numerous threats including diseases, chemicals, and mite infestations, causing widespread concern. While extensive research has explored the link between gut microbiota and their hosts. However, the impact of Varroa destructor infestation remains understudied. In this study, we employed massive parallel amplicon sequencing assays to examine the diversity and structure of gut microbial communities in adult bee groups, comparing healthy (NG) and Varroa-infested (VG) samples. Additionally, we analyzed Varroa-infested hives to assess the whole body of larvae. Our results indicated a notable prevalence of the genus Bombella in larvae and the genera Gillamella, unidentified Lactobacillaceae, and Snodgrassella in adult bees. However, no statistically significant difference was observed between NG and VG. Furthermore, our PICRUSt analysis demonstrated distinct KEGG classification patterns between larval and adult bee groups, with larvae displaying a higher abundance of genes involved in cofactor and vitamin production. Notably, despite the complex nature of the honeybee bacterial community, methanogens were found to be present in low abundance in the honeybee microbiota.

• 한방재활의학과학회지
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• 제27권1호
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• pp.53-65
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• 2017

Objectives The purpose of this study is to evaluate the safety of Non-toxic bee venom (BV) and observe VAS change before and after Non-toxic BV treatment in pain patients. Methods We surveyed the clinical practitioners who treated with Non-toxic BV in pain patients who visited the Korean medical clinic. The questionnaire survey was conducted for clinical practitioners who agreed to participate after hearing the explanation for the purpose and characteristics of the questionnaire. Patients in the questionnaires were reviewed based on their medical records from July 1, 2016 to October 28, 2016. Results We received 445 cases and selected 403 cases finally. 2 cases, however, were not able to continue treatment for 3 weeks and were eliminated. Depending on when the pain occurred, we divided the 401 cases into three groups (Acute, Subacute, Chronic group). In all groups, VAS scores were significantly decreased after treatment. Adverse reactions following Non-toxic BV treatment had occurred was 16 cases (3.60%). Except for 3 cases with hives, most of adverse reactions were mild or moderate and were not in need of extra treatment. The total safety of treatment for 3 weeks was mostly safe. The number of cases discontinued treatment was 42 cases (9.44%). Most of these cases, treatment was stopped for personal reason unrelated to the Non-toxic BV treatment. Conclusions These results suggest that the Non-toxic BV treatment has no serious adverse reactions and is a relatively safe treatment. Further studies are needed to prove the efficacy and clinical safety of Non-toxic BV treatment.

• 동의생리병리학회지
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• 제25권5호
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• pp.887-893
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• 2011

The purpose of this study was to investigate the wound healing effects of Danggwieumja (DG), which is commonly used for skin inflammation, skin wound, skin pruritus, and chronic hives etc. The 1.5 cm ${\times}$ 1.5 cm full-thickness skin wound was induced to two groups, DG (n=16) and Saline (n=16) group. The DG extract and Saline were orally administrated daily for 15 days after skin wound induction. Then, the body weight of rats and the congestion indices were daily measured for 15 days after skin wound induction. The wound contractions and epithelizations were also measured. The wound contractions were daily measured for 15 days after wound induction and wound epithelizations were measured for 8 days from day 7 after wound induction. For evaluating angiogenesis, the immunoreactivities of vWF and VEGF protein were measured immunohistochemistrically on day 15. In results, although the percentage increases in mean body weight of rats in the DG and Saline groups hve no significant differences, DG extract decreased the time of wound healing and congestion around wound, and improved wound contraction and epithelization. The contraction percentage of DG group was significantly increased on day 5 (P<0.05) and day 7 (P<0.01) than that of Saline group. DG group showed significant increase of wound epithelization on day 7 (P<0.05) as compared to Saline group. Moreover, DG extract reduced the inflammation of skin dermis and promoted the growth of vascular vessels of dermis by accelerating vascular endothelial growth factor (VEGF) protein. These results suggest that DG has the beneficial effects on skin incision wound and can be the suitable wound healing agent for various surgical wounds.

• 한국약용작물학회지
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• 제19권6호
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• pp.446-455
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• 2011

Galgeun-tang (GGT) is a traditional medicinal formula that is widely prescribed to treat cold, asthma, and hives in Korea. Fermented herbal medicines can be made more effective than normal herbal medicines by increasing the absorption and bioavailability of the active compounds. In this study, we fermented Galgeun-tang to produce bioconversion constituents using Lactobacillus plantarum (GGT144), and found that four peaks were decreased, three peaks were increased and two new peaks appeared in the HPLC-DAD chromatogram. After HPLC-DAD-guided fractionation of the newly-appearing compounds (1 and 5) and the increased (6, 7, and 9) compounds, the structure of the compounds was determined using NMR and MS. Using this approach the compounds were identified to be pyrogallol (1), daidzein (5), liquiritigenin (6), cinnamyl alcohol (7), and formononetin (9), respectively. In addition, the decreased compounds were identified to be daidzin (2), liquiritin (3), ononin (4), and cinnam aldehyde (8) using HPLC-DAD analysis with standard compounds. The high performance liquid chromatography method was used to quantify the nine constituents in GGT and GGT144. All calibration curves of the standard compounds displayed excellent linearity with a $R^2$ > 0.9968.

• 한국식품영양과학회지
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• 제32권5호
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• pp.689-694
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• 2003

꿀벌의 벌집에서 채집되어지는 프로폴리스는 항염증, 항암성 등의 민간의학으로 사용되어져 왔다. 이러한 프로폴리스를 이용하여 총당에 대한 포도당 함량(0~100%), 에탄올 농도(20~100%) 및 에탄올 용액 분무량 (6~10%)의 비율로 혼합하여 세립형성에 따른 수율, 교반에 의한 분쇄율 및 관능적 특성의 변화를 검토하였다. 수율의 경우 에탄올 함량이 감소할수록 수율이 높게 나타났으며, 분쇄율의 경우 에탄올 함량이 증가할수록 감소하였다 색, 향, 맛, 입안에서의 느낌 및 전반적인 기호도에 있어서는 총당에 대한 포도당의 함량, 에탄올 농도 및 에탄올 함량 모두에 영향을 받는 것으로 나타났으며, 전반적인 기호도는 총당에 대한 포도당 함량 47.94%, 에탄올 농도 56.45%및 에탄올 용액 분무량 8.04%일 때 관능적으로 가장 우수한 6.90의 평점이 나타났다.

• 대한한의학회지
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• 제37권1호
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• pp.135-150
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• 2016

Research Methods: This research analyzed the data on those aged 20 and older from the Report on Korean Medicine Usage Research, which was conducted in 2011. The definitions of toxicity were defined by the presence of toxicity listed in the survey. The questions used in analysis were sex, age, household income, health insurance, medical fees, satisfaction rates on treatments, as well as the types of diseases and the presence of toxicity from treatments. The analysis was done through frequency analysis using SAS 9.2 and Fisher's Exact Test. Results: Toxicity occurred in 2.1% patients out of the 3518 studied. The types of toxicity were skin problems, such as hives and pruritus (34.7%), gastrointestinal problems (20.8%), neurological diseases (4.2%), liver(1.4%), kidney toxicity(1.4%), and others (22.2%). There were no differences in toxicity by sex, age, household income and the types of health insurances. However, toxicity were positively correlated with the increase in standard of education (p=0.0124). In addition, as treatment costs increased (p<0.0001) and satisfaction rates decreased, toxicity increased (p<0.0001). Toxicity increased in patients with low back pain (p=0.0429), hwabyung (p=0.0392), lumbar sprain (p=0.0004), correction body type (p=0.0118), growth (p=0.0045), and from motor accidents (p=0.0448). In logistic regression analysis, Toxicity were positively correlated with medical fees, and cancer treatment and negatively correlated with satisfaction rate on treatments. Conclusion: The toxicity that occurred in outpatients who used Korean medical clinics mostly happened in skin, digestive organs, nerves, livers, and kidneys toxicity. The occurrences differed by the Educational lengths, expensive costs of treatments, low satisfaction rates of the treatments, and cancer.