• Fisheries and Aquatic Sciences
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• v.18 no.4
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• pp.349-357
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• 2015

To reutilize fisheries waste, we isolated a bacterial strain from a coastal area located in Busan. It was identified as Bacillus licheniformis TK3-Y. Using plate assay and 500-mL flask experiments, we found that the isolate simultaneously possessed cellulolytic, proteolytic, and lipolytic activities with salt tolerance. 10% (v/v) inoculums, were used to examine the biodegradation characteristics of the TK3-Y strain on carboxymethylcellulose, skim milk, and olive oil media. The optimum conditions for pH, temperature, agitation speed, and NaCl concentration on each 1% substrate were 6, $50^{\circ}C$, 180 rpm, and 17.5%, respectively. Under optimal conditions, the TK3-Y strain showed 1.07 U/mL cellulolytic, 1,426 U/mL proteolytic, and 6.45 U/mL lipolytic activities. Each enzyme was stable within a range of 17.5-35% NaCl. Therefore, the salt tolerance ability of strain TK3-Y was superior to other related strains. In degradation of a mixed medium containing all three substrates, both the cellulolytic and proteolytic activities were somewhat lower than those on each single substrate, while the lipolytic activity was somewhat higher. From the above results, the TK3-Y strain appears to be a good candidate for use in the efficient treatment of fisheries waste in which components are not collected separately.

• Journal of Family Resource Management and Policy Review
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• v.3 no.1
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• pp.1-15
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• 1999

The purpose of this study is to find out the tendency of the new generation’s consumer roles and green consumption behavior for the environmental conservation. This study is also to reveal some fundamental information for an environmental education program which si able to support the new generation consumers to participate voluntarily in an environmental activity. The 500 subjects of this study were selected from the new generation consumers who wre residing in Seoul, 1998. The 414 data wre finally analyzed by Mean, t-Test, ANOVA, and Multiple Regression Analysis. The major findings were as follows: 1) the purchasing roles of the new generation consumer tended to be higher than the citizen participation’s roles. The variables with the higher score for the new generation’s green consumption behavior were in the rank of the isolation of environmental waste, the purchasing behavior of green product, the recycling environmental resource, and citizen’s participating roles in environmental activity. 2) the new generation’s consumer roles showed a significant differences, according to variables for the environmental value, environmental education, and participation of the environmental and societal activity 3) the green consumption behavior for the new generation consumers showed a significant differences, according to sex, environmental value, participation of the environmental and societal activity. 4) the most influential variables for the green consumption behavior of the new generation consumers were in the rank of consumer roles, educational values, and the participation of the environmental activity. As we see above, the consumer education for the new generations needs to focus on consumer’s roles that let them participate voluntarily in environmental and societal activity, as well as practice positively to use green environmental products whenever they select some items in the markets.

• Journal of the Korea Organic Resources Recycling Association
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• v.10 no.3
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• pp.126-131
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• 2002

For the probiotic feed production from residual food waste, aerobic liquid fermentation was conducted by thermophilic bacteria. 11 Strains of bacteria were isolated from several soil sources and residual food waste. Screening was carried by shaking incubator for the separation of thermophilic strain at $55^{\circ}C$. The isolated strains were tested for enzyme activities such as ${\alpha}$-amylase and protease. 6 Bacterial strains were chosen and were adapted by repeated fermentation processes in food waste substrate. The viable cell count of them at final fermentation stages were shown as $3-7{\times}10^9/ml$ in 2L-jar fermenter. Among them B3, B6 showed higher enzyme activity. By the mixed fermentation of B3, B6 and Bacillus stearothermophilus, the highest viable cell count reached to $1.4{\times}10^{10}/ml$ in 8 hours.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.11
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• pp.1617-1622
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• 2006

Long-term storage of feeds or feedstuffs in high temperature and humid conditions can be difficult because of microbial contamination. Essential oil isolated from industrial waste citrus peel could be used as a preservative because it is likely to have anti-bacterial and anti-fungal activity. Our objective was to determine whether different levels (0.028, 0.056 and 0.112 g/kg) of citrus essential oil (CEO) would provide anti-microbial activity and enhance preservation of animal feed without influencing rumen fermentation. At 0.112 g/kg, CEO inhibited growth of Escherichia coli (ATCC 25922) and Salmonela enteritidis (IFO 3313). Growth of E. coli recovered after 24 h of incubation, but S. enteritidis continued to be inhibited for 72 h. Preservation of antibiotic-free diets for swine was assessed by observing anti-aflatoxin activity. Aflatoxin was detected in control feed samples on days 16 (8 ppb) and 21 (8 ppb) and in anti-fungal agent (AA) treated samples on days 16 (2 ppb) and 21 (4 ppb). However, aflatoxin was not detected in feed samples treated with CEO. Treatment with CEO and AA did not influence ruminal pH, dry matter digestibility (DMD) or organic matter digestibility (OMD) over 48 h of incubation in rumen fluid. Acetate and propionate were slightly higher with CEO treatment (p<0.05), but total concentration of volatile fatty acid (VFA) was not significantly affected by treatment. Ammonia-N concentration was slightly higher for the control treatment (p<0.05). This study showed that treating feed with CEO enhances preservation of animal feed without influencing in vitro rumen fermentation.

• Clean Technology
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• v.29 no.4
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• pp.321-326
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• 2023

Recently, there has been a growing interest in clean hydrogen energy that does not emit carbon dioxide during combustion due to the increasing focus on carbon neutral. Research related to hydrogen production continues, and in this study, we applied waste-derived synthesis gas to the water-gas shift reaction to simultaneously treat waste and produce high-purity hydrogen. To enhance catalytic activity in the high-temperature water-gas shift (HT-WGS) reaction, magnesium was used as a support material alongside cerium. Cu-CeO₂-MgO catalysts were synthesized, with copper acting as the active component for the HT-WGS reaction. A study on the catalytic activity based on the preparation method was conducted, and the Cu-CeO₂-MgO catalyst prepared by impregnation method exhibited the highest activity in the HT-WGS reaction. The observed superior performance of the Cu-CeO₂-MgO catalyst prepared through the impregnation method can be attributed to its significantly higher oxygen storage capacity and amount of active Cu species.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.6
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• pp.825-828
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• 2003

This study was performed to determine the antimicrobial activity of waste food fractions, the Solanum tuberosum Peel (SP) and Allium cepa L. Peel (AP) on several microorganisms. The methanol extracts (SPM, APM) of SP and AP were fractionated to five different types, which were hexane, ethylether, ethylacetate, butanol and water Among the various fractions, ethylether and butanol fractions of AP (APMEE, APMB) showed stronger antimicrobial activity against Pseudomonas aeruginosa and Staphylococcus aureus than the other fractions. The ethylether fraction of SP (SPMEE) also showed the higher antimicrobial activity against Escherchia coli than the other 4 fractions . These results suggest that the waste food fractions, SP and AP may be developed into bioactive antimicrobial health products.

• Journal of Nutrition and Health
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• v.22 no.1
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• pp.1-8
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• 1989

The study was attempted to observe effects of voluntary exercise on energy expenditure and on storage of excessive energy into body fat in adult mice. Mice, body weight 25-30g, were divided into two groups, exercise and sedentary group. Voluntary exercize by running wheel was allowed for former group but not for the mice of the latter group which were restricted by limiting space allowance 13.5$\times$11.5$\times$15.0cm per mouse. During a period of 4 weeks of feeding trial, they were fed ad libitum starch-casein based diet added with $^3\textrm{H}$-glucose (D-[1-$^3\textrm{H}$(N)]-glucose) at a level of 20 nCi per g of diet. Measurements were made to study hematology, lipase activity in epididymal adipose tissue, total contents of body waste and fat, and radiactivity of $^3\textrm{H}$-glucose incoporated into body fat. Dietary intake, body weight gain and amount of voluntary physical activities were also measured. The results obtained ard summarized as follows; 1) Amount of metabolizable energy intake, body weight gain and body fat were not statistically different between the two group. However, mice of the exercise group tended to show lower body weight gain body fat contants, but higher energy expenditure than those of the sedentary group. 2) Radioactivity of $^3\textrm{H}$-glucose incoporated into body fat appeared lower for exercise group expressd on a whole body fat basis. The activity was, however, higher for exercise group when expressed based on per g of fat compared to that of sedentary group. 3) Exercise group showed also higher activity of lipase in epididymal adipose tissue than the other group. 4) Mice whose physical activities were restricted appeared to have lower levels of hemoglobin and hematocrit values than of the exercise group. These results seem to support the theory that turn-over rate of body fat is activated by exercise and to suggest that consumed energy is to be converted primarily into body fat before its use as energy source by oxidation even during a period of continuous energy expenditure by exercise.

• Korean Journal of Microbiology
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• v.21 no.2
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• pp.51-60
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• 1983

Cultural characteristics of Strptomyces griseolus isolated from the soil were investigated. This strain was disclosed to utilize D-xylose, and D-glactose in preference order as a carbon source with the formation of glucose isomerase. The addition of sweet potato starch also proved effective promoting the total enzyme activity measured at 29% higher than the control. Corn cob, one of waste agricultural resources, was hydrolyzed in 2~3% $H_2SO_4$ solution at $100^{\circ}C$, 3~5 hours to produce a xylose syrup which gave rise to the recovery of 19.9% in a batch system and 28.2% in a repeated system. By the addition of both 2% of xylose syrup(Be'28) prepared by and us 65% of corn steep liquor (total nitrogen 1.2%), enzyme induction was maximized. The enzyme activity was stimulated by the xylose and the cell growth by the C.S.L. Also, remarkable increase of enzyme activity was noticed by the addition of protein acid hydrolysate 86.2% higher than the control. $QO_2$ of the biomass cultured in 30L capacity jarfermentor recorded low oxygen requirement of 251.2 1/hr. Maximum activity of glucose isomerase was observed noted at the 9th hour after inoculation which is 2 hours faster than the stationery was observed noted at the 9th hour after inoculation which is 2 hours faster than the stationery phase of the biomass growth. Glucose isomerase from the strain was activated by adding the $Co^{++}\;and\;Mg^{++}$ with optimum temperature of $73^{\circ}C$ and pH of 7.2. Conversion ratio of 60% glucose to frutose was 42.5% after 70 hours reaction.

• Electronic Materials Letters
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• v.14 no.6
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• pp.739-748
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• 2018

ZnS nanowalls, microspheres and rice-shaped nanoparticles have been successfully grown on graphene oxide (GO) sheets by the hydrothermal method. The morphologies, structures, chemical compositions and optical properties of the as-synthesized GO/ZnS have been characterized by X-ray power diffraction, energy dispersive spectrometer, scanning electron microscope, Raman spectra, photoluminescence spectroscopy and ultraviolet-visible absorption spectroscopy. It was found that the concentration of CTAB and the reaction temperature were important in the formation of GO/ZnS microstructures. The photocatalytic activity of the as-synthesized GO/ZnS was investigated through the photocatalytic degradation of textile dyeing waste. Results showed that the catalytic activity of the GO/ZnS porous spheres to methyl orange and methylene blue is higher than those of other samples. The degradation rates of methyl orange and methylene blue by porous spheres in 50 min were 97.6 and 97.1%, respectively. This is mainly attributed to the large specific surface area of GO/ZnS porous spheres and high separation efficiency between photogenerated electron and hole pairs.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2005.11b
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• pp.165-175
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• 2005

Measurement of spent resin activity was initiated in 2004 in order to develop the C-14 removal technology for safe disposal. As part of this program, spent resins were sampled and measured in the in-station resin storage tank 2 at Wolsong Nuclear Power Plant Unit 1. At the time of sampling, the resins had been in storage tank from 3 to 23 years. Total 72 resin samples were sampled, which were collected from both man-hole (68 samples) and test-hole (4 samples) in the in-station resin storage tank 2. They were separated into liquid, activated carbon, zeolite, and spent resin. The spent resins were oxidized with sample oxidizer and analyzed for C-14. Ten of collected mixed resin samples were separated by density into cation and anion resins using a sugar solution. The C-14 concentration in anion exchange resin was approximately 2 times higher than in the mixed resin. The average concentration of C-14 in the cation/anion mixed exchange resin was $460\;GBq/m^3$ from test-hole and $53.1\;GBq/m^3$ from man-hole. We have found that concentration of C-14 in the spent resin is about from 0.4 to $1,321\;GBq/m^3$. So it could be a problem, when dispose of at a repository, since there is a disposal limit of $222\;GBq/m^3$. This means we should develop the C-14 removal technology.