• Journal of The Korean Society of Grassland and Forage Science
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• v.26 no.1
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• pp.25-30
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• 2006

This experiment was conducted to compare the fresh yield and dry matter yield, and nutritive values of whole crop barley treated with Urea (200 kg/ha, T2) and ammonium sulfate fertilizer as 200 kg/ha (T3), 300 kg/ha (T4) and 400 kg/ha (T5) at the Bae-Ho reclaimed tideland. Korea from 2003 to 2005. Salt contents of soil in the ammonium sulfate plots (T3, T4, T5) were lower than those of T2. The fresh yields of ammonium sulfate plots (T3, T4, T5) were higher than those of T2 as 62% (p>0.05), 41% (p>0.05) and 23% (p<0.05), respectively. The dry matter yield of T3, T4 and T5 (ammonium sulfate) was significantly (p<0.05) higher at 5,080 kg/ha, 4,667 ka/ha, 4,040 kg/ha, respectively. The total digestible nutrients (TDN) yield tends to have a similar result. Crude protein (CP) content was highest in T3 and CP trends to decrease as the level of ammonium sulfate was increased. Total digestible nutrient (TDN) were high in T3 and T4. The sodium content of T3 and T4 were lower than T2. Based on the study, it was more desirable to use ammonium sulfate (200 kg N/ha) rather than Urea as fertilizer on reclaimed land in terms of forage production and nutritive value.

• Journal of Wetlands Research
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• v.15 no.2
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• pp.251-264
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• 2013

There are some factors affecting the shorebirds in selecting a coastal inland roost site where they have to stay during the high tide when the entire intertidal zone is covered with water. We investigated eight species (Eastern Curlews Numenius madagascariensis, Green Shanks Tringa nebularia, Bar-tailed Godwits Limosa lapponica, Grey Plovers Pluvialis squatarola, Dunlins Calidris alpine, Whimbrels Numenius phaeopus, Eurasian Curlews Numenius arquata and Terek Sandpipers Xenus cinereus) to identify the spatial and temporal variability in coastal inland roost use in the area and the factors influencing the use. We considered the area and length of the standing water in the roost site, temperature, wind speed, the time of migration and the intensity of disturbance. As a result, number of individuals and type of species using the roost site varied across spatial and temporal scales. And the factors affecting the roost use was species-specific. The roost site of the study area was a closed shrimp farm however, it has been converted to a Salicornia herbacea, a salt marsh plant, farm recently. In a situation where coastal inland roost site is needed, we hereby describe a resolution for the juxtaposition of shorebirds and farming.

• Korean Journal of Environmental Biology
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• v.21 no.3
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• pp.313-318
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• 2003

Environmental stresses, such as heat shock, alcohol and physiological salt have been shown to induce a group of protein called heat shock protein (HSPs) in various tissues. In this investigation, we studied that arsenic stress would alter contraction of isolated rat aorta and expression of heat shock protein 70 and investigated the relation between expression of HSP 70 and vascular contractility of isolated rat aorta. Rat aorta strips, mounted in organ baths were exposed to 0, 0.5, 1,2 and 4 mM arsonic for 60 min. and 1,3 and 8 hours later tested for contractile response and expression of heat shock protein 70. Contractility of rat aorta were determined by isometric transducer connected to computerized physiograph and expression of HSP 70 was characterized by western blotting, respectively. Potassium chloride (55 mM) significantly augmented vascular contractility of yat aorta by 39％ compared with the control at 8 hours but not one or three hours after treatment of 4 mM arsenic. Arsonic stress (4 mM) also increased the expression of HSP 70 in rat aorta at 8 hours but one or three hours compared with the control and HSP expressed in vascular smooth muscle cells and some expressed in endothelium cells. These results suggest that arsenic stress not only did alter the magnitude of the contractile response to high potassium chloride but also increased the expression of HSP 70 in the rat aorta.

• Journal of the Korean Institute of Landscape Architecture
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• v.36 no.3
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• pp.52-62
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• 2008

By analyzing specific plant distributions and physicochemical characteristics of topsoil in a reclaimed dredging area, baseline data was found of natural landscape planting sites, and developing dredged fill ground. The reclaimed dredging area is five different stands (1, 2, 3, 4 and 5) which were examined in this research. They are located from sea level to 15 meters in altitude and exhibited typical characteristics of the salt marsh in Gwangyang Bay. Species with high constancy in the vegetation on the reclaimed soil were classified into four stages. A total of 12, 15, 22, 27 and 35 different plant species were found and also increased in stands 1, 2, 3, 4 and 5, respectively. Moving from stand 1 to 5, halophytes decreased and non-halophytes increased. Desalination at each stage of the reclaimed dredging area was a driving force affecting the performance and distribution of halophytes and non-halophytes. Overall, 35 quadrats of soil were selected and analyzed for specific physicochemical characteristics of topsoil between O${\sim}$20cm. Results of the physicochemical analysis such as altitude, slope, vegetation and kind of reclaimed dredging soil, exhibited irregular increases or decreases. As survey areas moved from stand 1 to 4, desalination areas, soil acidity, electric conductions, content of salinity, available phosphorus, potassium, chlorine, calcium, and magnesium indicated decreasing patterns; however, total nitrogen, silt, and clay content increased. Cluster analysis and PCA by environmental data within the stands clearly showed five distinct vegetation patterns on the tested reclaimed area. These results indicate that the differences of performance and distribution of vegetation are due to the SAR in the reclaimed soil and related to the natural survival strategy at the given hostile habitat.

• Journal of the Korea Organic Resources Recycling Association
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• v.6 no.2
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• pp.59-67
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• 1998

This study was conducted to investigate the changes of soil chemical properties and uptake of salts by the plants(Raphanus and Lactuca) according to the application of the food waste compost(FWC), which was produced on a large scale at a pilot plant. Generally, FWC has a high electrical conductivity(EC) and contains much salts, such as Na, K, Ca, and Mg, and because of these when FWC are applied to soils there is a possibility of salt ac-cumulation in soils and growth inhibition on plants. The FWC were applied at the rates of 0, 20, 40, 80, and 160 Mg/ha in the 1/5,000a pots on the basis of dry weight, and the plants were cultivated for 60 days. And then, changes of pH and EC in soils and uptake of salts by the plants were investigated. The results obtained are summarized as follows; 1. After the cultivation of the Raphanus and Lactuca, pH increased and EC decreased in the soils. 2. Uptake rates of Na and K were slightly increased with the application of the FWC. In uptake rate of Ca, Raphanus and Lactuca was decreased, increased, respectively. In Raphanus, uptake rate of Mg was highest with the application of the FWC at 40 Mg/ha, and in Lactuca, continuously increased with application of the FWC.

• Korean Journal of Community Nutrition
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• v.18 no.4
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• pp.365-371
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• 2013

High intakes of sodium may increase the risk of hypertension or cardiovascular diseases. According to the 2010 Korea National Health and Nutrition Survey, the average intake of sodium was 4,878 mg/day with salt, kimchi, soy sauce, fermented soybean paste and Ramyeon being the five main sources of sodium. In order to identify solutions to reduce the intake of sodium, we investigated the intake patterns and eating behaviors of Ramyeon among 347 college students (male 146, female 201) using survey questionnaires. The average age of study subjects was 23.7 years for males and 20.5 years for females. The average Body Mass Index ($kg/m^2$) was 21.9 for males and 20.1 for females. The average frequency of Ramyeon intake was 2.0 times/week. The main reason for eating Ramyeon was convenience (56%), followed by good taste (27%), low price (11%) and other reasons (9%). The criteria for choosing Ramyeon were taste (72%), convenience (14%), price (7%), nutrition (1%), and the other factors (2%). Males' average intake of Ramyeon soup (61%) was higher than that of the females (36%). The estimated intake of Ramyeon soup by survey showed a positive correlation with the measured intake of Ramyeon soup. Sodium contents of Ramyeon were measured separately for the noodles and the soup, which were 1,185 mg/serving and 1,148 mg/serving each. Therefore, the amount of sodium intake can be reduced if students eat less Ramyeon soup. Also, we observed that dietary behaviors and soup intakes of Ramyeon between the sexes were different. Appropriate nutritional education for proper eating habits may help decrease the intake of sodium.

• Journal of Microbiology and Biotechnology
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• v.24 no.5
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• pp.597-604
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• 2014

$\small{D}$-Phenylglycine aminotransferase ($\small{D}$-PhgAT) from Pseudomonas stutzeri ST-201 is useful for enzymatic synthesis of enantiomerically pure $\small{D}$-phenylglycine. However, its low protein solubility prevents its application at high substrate concentration. With an aim to increase the protein solubility, the N-terminus of $\small{D}$-PhgAT was genetically fused with short peptides ($A_1$ ${\alpha}$-helix, $A_2$ ${\alpha}$-helix, and ALAL, which is a hybrid of $A_1$ and $A_2$) from a ferredoxin enzyme of a halophilic archaeon, Halobacterium salinarum. The fused enzymes $A_1$-$\small{D}$-PhgAT, $A_2$-$\small{D}$-PhgAT, and ALAL-$\small{D}$-PhgAT displayed a reduced pI and increased in solubility by 6.1-, 5.3-, and 8.1- fold in TEMP (pH 7.6) storage, respectively, and 5-, 4.5-, and 5.9-fold in CAPSO (pH 9.5) reaction buffers, respectively, compared with the wild-type enzyme (WT-$\small{D}$-PhgAT). In addition, all the fused $\small{D}$-PhgAT displayed higher enzymatic reaction rates than the WT-DPhgAT at all concentrations of L-glutamate monosodium salt used. The highest rate, $23.82{\pm}1.47$ mM/h, was that obtained from having ALAL-$\small{D}$-PhgAT reacted with 1,500 mM of the substrate. Moreover, the halophilic fusion significantly increased the tolerance of $\small{D}$-PhgAT in the presence of NaCl and KCl, being slightly in favor of KCl, where under the same condition at 3.5 M NaCl or KCl all halophilic-fused variants showed higher activity than WT-$\small{D}$-PhgAT.

• Microbiology and Biotechnology Letters
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• v.47 no.4
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• pp.574-580
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• 2019

ATP drives the conformational change of the group II chaperonin from the open lid substrate-binding conformation to the closed lid conformation to encapsulate an unfolded protein in the central cavity. It is thought that the folding activity of group II chaperonin is strongly correlated with the ATP-dependent conformational change ability. In order to confirm the dependence of the reaction temperature and ATP concentration of PhCpn, the ATPase activities were measured under different reaction temperatures and ATP concentrations. The maximal ATPase activity of PhCpn was observed at 80℃ and 3 mM ATP concentration. As a result of ATPase activity according to the type of salt ions, the highest activity was observed at 300 mM LiCl among the univalent cations and 5 mM MgCl₂ among the divalent cations, respectively. The values of Km and Vmax for ATP substrate were estimated as 2.17 mM and 833.3 μM/min, respectively. This results provide the enzymatic information of PhCpn when the prolonged and high activities of pharmaceutical and industrial proteins (or enzymes), by using chaperonin molecules, are required.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.3 no.2
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• pp.105-112
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• 2005

The electrolytic reduction of uranium oxide in a LiCl-Li$_{2}$O molten salt system has been studied in a 10 g U$_{3}$O$_{8}$ /batch-scale experimental apparatus with an integrated cathode assembly at 650$^{\circ}C$. The integrated cathode assembly consists of an electric conductor, the uranium oxide to be reduced and the membrane for loading the uranium oxide. From the cyclic voltammograms for the LiCl-3 wt$\%$ Li$_{2}$O system and the U$_{3}$O$_{8}$-LiCl-3 wt$\%$ Li$_{2}$O system according to the materials of the membrane in the cathode assembly, the mechanisms of the predominant reduction reactions in the electrolytic reactor cell were to be understood; direct and indirect electrolytic reduction of uranium oxide. Direct and indirect electrolytic reductions have been performed with the integrated cathode assembly. Using the 325-mesh stainless steel screen the uranium oxide failed to be reduced to uranium metal by a direct and indirect electrolytic reduction because of a low current efficiency and with the porous magnesia membrane the uranium oxide was reduced successfully to uranium metal by an indirect electrolytic reduction because of a high current efficiency.

• Applied Biological Chemistry
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• v.46 no.2
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• pp.94-100
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• 2003

Preparation method and characteristics of high quality Korean pickled cucumber made with acetic acid-treated cuttle bone powder (KPCCP) were examined and compared to those of Korean pickled cucumber prepared by traditional methods (control). Optimal concentration of acetic acid-treated cuttle bone powder for KPCCP preparation was revealed as 10%. Salt concentration of KPCCP fermented for 3 days was 4.5%, slightly higher than that of the control, whereas that of KPCCP fermented for over 6 day was about 4.8%, similar to that of the control. The pH, greenness, and hardness of korean pickled cucumber decreased during fermentation, with the control showing a greater extent of decrease than KPCCP Total acidity and alcohol insoluble calcium contents increased during fermentation, with KPCCP showing higher extent of increase. These results indicate that quality of KPCCP is superior to that of Korean pickled cucumber prepared by traditional methods.