• 대한수의학회지
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• 제40권1호
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• pp.42-48
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• 2000

Akabane disease is transmitted through mosquitoes in cattle, sheep and goats. It shows congenital abnormalities including encephalomyetitis, hydranencephaly, neurogenic arthrogryposis, and deformed neonatal calves. Akabane viruses, 93FMX and K-9 strain, were isolated from fetal matrix of aborted cow and blood of healthy cow, respectively. S gene sequences of 93FMX and K-9 showed 100% homology with that of OBE-1 strain isolated in Japan. Based upon our sequencing data, we synthesized specific primers for PCR diagnosis. Using these primers, we were able to amplify the S gene of Akabane virus not only from the culture fluid of Vero cells but also from the brain tissue of suckling mouse inoculated with, Akabane virus. These PCR products were confirmed by Southern blot hybridization. Not only the sensitivity of PCR test was high enough to detect the viruses of $10^{1.0}TCID_{50}/ml$, but also the time for diagnosis was significantly shorter than that of the virus isolation by tissue culture method. This method was also effective for the detection of Akabane virus in the cerebrum of fetus. RT-PCR method may be used for a useful diagnostic test of the clinical cases of Akabane disease.

• 대한미생물학회지
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• 제22권4호
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• pp.393-402
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• 1987

The halophilic bacterium Vibrio vulnificus, previously called lactose-positive(L+) Vibrio and Beneckea vulnifica, causes acute, fulminating wound infections and septicemia in humans. Septicemia is very serious infection with a fatality rate of about 50%. Most patients with primary septicemia due to V. vulnificus have preexisting liver disease. V. vulnificus also cause severe wound infections usually after trauma and exposure to marine animals or the marine environment. The mortality rate is not nearly as high as in primary septicemia caused by this organism. In most cases human disease results from ingestion of contaminated seafood or from infection of a wound, frequently of seawater or crab origin. The author made an attempt to isolation of the V vulnificus from seawater, seamud, fish, shellfish, and algae on the southern sea of Korea from January to September in 1987, using for the purpose of vaccine preparation. The author investigated for bacteriological identification, hemolysis and determination of serotypes of isolated V. vulnificus strains. Eighty-five strains(5.9%) out of 1450 specimens collected of V. vulnificus were isolated. The distribution of the 85 isolates were as follows: 21 strains from seawater, 11 strains from seamud, 28 strains from fish, 19 strains from shellfish, and 6 strains from algae, respectively. All 85 isolates were positive reaction on human blood agar. The distribution of serotypes of V. vulnificus isolates were O1 to O8: 13 strains of O1, 6 strains of O2, 11 strains of O3, 9 strains of O4, 10 strains of O5, 7 strains of O6, 15 strains of O7, and 10 strains of O8, respectively. Eighty-one strains showed agglutination with O antisera, but 4 strains failed to show agglutination. In this study, the author suspected that serotypes of V. vulnificus isolates distributed also in the seaside of Korea as well as in most seaside of the world, and new serotypes were in existence in the seaside of Korea except reported up to now.

• 한국광학회지
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• 제13권6호
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• pp.461-466
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• 2002

파장분할 다중화 시스템의 다중 채널분리 필터가 측면연마 광섬유 결합기를 이용한 빗살필터로 구현됨을 제안하였다 단일 모드 광섬유의 기본모드와 결합하는 상부 평면 도파로의 모드가 고차일수록 광 전력전달 효율은 낮아지는데, 두 도파로 사이 에 중간 결합층을 삽입함으로서 개선시킬 수 있고 최적화 조건에서 20dB 이상의 채널간 소멸비를 얻을 수 있음을 보였다. 200fm두께의 리튬나오베이트를 상부 평면 도파로로 적용한 경우 4m의 채널간격을 가지는 빗살필터 특성을 얻었으며, 중간 결합층의 두께가 1 Um일 때 굴절률이 1.52-1.53 범위에서 최대 광 전력전달이 일어남을 관측하였고 빔 전파 방법을 이용하여 계산한 결과와 거의 일치함을 보였다.

• Structural Engineering and Mechanics
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• 제24권3호
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• pp.375-393
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• 2006

Under high velocity, pulse type near source earthquakes semi-active control systems are very effective in reducing seismic response base isolated structures. Semi-active control systems can be classified as: 1) independently variable stiffness, 2) independently variable damping, and 3) combined variable stiffness and damping systems. Several researchers have studied the effectiveness of independently varying damping systems for seismic response reduction of base isolated structures. In this study effectiveness of a combined system consisting of a semi-active independently variable stiffness (SAIVS) device and a magnetorheological (MR) damper in reducing seismic response of base isolated structures is analytically investigated. The SAIVS device can vary the stiffness, and hence the period, of the isolation system; whereas, the MR damper enhances the energy dissipation characteristics of the isolation system. Two separate control algorithms, i.e., a nonlinear tangential stiffness moving average control algorithm for smooth switching of the SAIVS device and a Lyapunov based control algorithm for damping variation of MR damper, are developed. Single and multi degree of freedom systems consisting of sliding base isolation system and both the SAIVS device and MR damper are considered. Results are presented in the form of nonlinear response spectra, and effectiveness of combined variable stiffness and variable damping system in reducing seismic response of sliding base isolated structures is evaluated. It is shown that the combined variable stiffness and variable damping system leads to significant response reduction over cases with variable stiffness or variable damping systems acting independently, over a broad period range.

• 디지털융복합연구
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• 제15권4호
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• pp.427-441
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• 2017

본 연구의 목적은 문화예술교육에 대한 공감기대가치와 사회적 성향이 학습자의 통제된 기제 해소에 미치는 영향을 실증적으로 살펴보는데 있다. 4050세대 대상의 정량적 조사가 실시되었다. 연구 결과, 목표지향 공감기대가치-개인주의, 관계지향 공감기대가치-집단주의 간의 상관관계가 높은 것으로 나타났다. 아울러, 목표지향 공감기대가치는 자아효능감 현실화에, 즐거움 공감기대가치는 유보된 욕망 재점화에, 관계지향 공감기대가치는 탈고립 실제화에, 개인주의는 자아효능감 현실화에, 집단주의는 탈고립 실제화에 상대적으로 더 큰 영향을 미치는 것으로 나타났다. 본 연구는 문화예술교육 공감기대가치와 학습자의 사회적 성향, 그리고 학습자의 심리적 기제에 대한 학제적 융합적 이해의 필요성을 환기시키고, 나아가 이에 기반 한 프로그램 개발, 사회적 요구에 대처한 질적 양적 적합성 확보, 호혜성을 고려한 운영, 평가, 관리 등과 연계된 후속 연구와 실무적 적용이 지속적으로 필요함을 제시하고 있다.

• 미생물학회지
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• 제27권4호
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• pp.404-414
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• 1989

김치발효 중 Gram 양성세균 또는 유산균의 분리와 동정을 시도하였다. 종의 다양성은 분리배지와 온도에 영양을 받았고, 다양성은 온도가 낮아질수록 감소하는 경향이었다. MRS는 세균의 분리에 KM(김치재료로 만든 자연배지)은 종의 수를 파악 하는데 각각 적합하였다. 분리균의 동정은 Bergey's manual of Systematic Bacterio]ogy (1986)를 기초로 하여 작성한 이 분농생표(dichotomous Identification scheme)에 의하여 선행하였다. 각 온도(5, 15, $25^{\circ}C$)에서 동정된 Gram 양성세균은 Leuconostoc 5종, Streptococcus 4종, Pediococcus 3종, Bacillus 2종 그리고 Ltobacillms 18종이였다. 각 온도에서 출현 빈도가 높은 종은 $25^{\circ}C$에서 LactobaIlus ptantanmz, Streptococcµs faffinolactis, Leuconostoc maιnteroid, subsp mlsentιroides 이었고, $15^{\circ}C$에서 L. mesenteroides Lactobacillus fructosus, L. maen teroid,l.I subsp. mesent,roid, 이였고, $5^{\circ}C$ 에서 Leuconostoc sp.(65.2 빈도)에 의하여 이루어졌다. 그리고 각 온도에 따른 김치발효 중 지금까지 알려진 Pediococcus cerevisiae 와 Streptococcus faecalis는 분리되지 않았다.

• 한국조경학회지
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• 제29권6호
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• pp.37-49
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• 2002

The objective of this research was an investigation of the important green spaces in Daegu and to present the basic frame of green network in Daegu. Present the condition of the significant green spaces is as follows: In the 3km radius of the city center, the isolation and discontinuation of the green spaces was extensive, caused by crowding of houses and funeral parlours. Consequently, the isolation relaxation which leads to the additional creation of the green spaces and a buffering plan were demanded. In the transfer area of a 3∼6km radius, which is located between the city center's congested area and the suburb green spaces, the significant green spaces had high connection and buffering characteristic, but in the northwest and the south, serious discontinuation appeared between the suburb green spaces and the city conte green spaces. In the 6∼9km radius, the suburb important green spaces accomplished the function which extended the back core green spaces to the city center area. But creation of buffering green spaces was necessary in part because of proximity to the resident area. The results suggest a connection between green spaces on the base of the distribution of the important green spaces, were as follows: In the 3km radius, irregular direction lines appeared while a schedule one direction line appeared in 3∼6km and 6∼9km radius. The discontinued parts of the green network were caused by industrial complexes, house crowding, and cultivation areas. Consequently, reservation and creation plans of green spaces in response to this were demanded. Through the additional creation of green spaces in the discontinued parts, the basic frame of green network in Daegu was the radiation annular form. And it was suitable to activate the parks and the general green spaces.

• 한국항공우주학회지
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• 제44권1호
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• pp.40-48
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• 2016

관측위성의 고 해상도 임무요구조건 충족을 위해 기계적 구동부를 갖는 탑재장비로부터의 미소진동은 항상 차폐의 대상으로 존재하였다. 본 연구에서는 차폐의 대상이던 미소진동에 주목하여, 전기에너지 재생이 가능하고 동시에 진동절연이 가능한 복합 시스템 구현을 목표로 동조질량 흡진기 형태의 전자기 하베스터와 결합된 수동형 진동절연 시스템을 제안하였다. 아울러 하베스터의 기본특성 측정시험 결과에 기인한 수치해석과 미소진동시험 및 생성전력 측정시험을 통해 본 연구에서 제안한 복합시스템은 미소진동 절연과 동시에 전기에너지 재생에 유효함을 입증하였다.

• Computers and Concrete
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• 제16권4호
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• pp.503-529
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• 2015

Traditionally, multi-story buildings are designed to provide stiffer structural support to withstand lateral earthquake loading. Introducing flexible elements at the base of a structure and providing sufficient damping is an alternative way to mitigate seismic hazards. These features can be achieved with a device known as an isolator. This paper covers the design of base isolators for multi-story buildings in medium-risk seismicity regions and evaluates the structural responses of such isolators. The well-known tower building for police personnel built in Dhaka, Bangladesh by the Public Works Department (PWD) has been used as a case study to justify the viability of incorporating base isolators. The objective of this research was to establish a simplified model of the building that can be effectively used for dynamic analysis, to evaluate the structural status, and to suggest an alternative option to handle the lateral seismic load. A finite element model was incorporated to understand the structural responses. Rubber-steel bearing (RSB) isolators such as Lead rubber bearing (LRB) and high damping rubber bearing (HDRB) were used in the model to insert an isolator link element in the structural base. The nonlinearities of rubber-steel bearings were considered in detail. Linear static, linear dynamic, and nonlinear dynamic analyses were performed for both fixed-based (FB) and base isolated (BI) buildings considering the earthquake accelerograms, histories, and response spectra of the geological sites. Both the time-domain and frequency-domain approaches were used for dynamic solutions. The results indicated that for existing multi-story buildings, RSB diminishes the muscular amount of structural response compared to conventional non-isolated structures. The device also allows for higher horizontal displacement and greater structural flexibility. The suggested isolation technique is able to mitigate the structural hazard under even strong earthquake vulnerability.

• Journal of Genetic Medicine
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• 제17권2호
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• pp.68-72
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• 2020

Purpose: Trisomy 21, the cause of Down syndrome (DS) with various medical problems, is the most common aneuploidy during the fetal period. For diagnosis, a non-invasive screening test using maternal blood, which cannot be confirmed and invasive confirmation test with a risk of miscarriage, may be performed. The trophoblast retrieval and isolation of the cervix (TRIC) have been proposed by some researchers as an alternative to overcome the limitations of current tests. We experimented using TRIC to identify the possibility of trisomy 21 for the first time in Asia. Materials and Methods: Three cases of DS were analyzed confirmed by invasive tests (chorionic villus sampling, amniocentesis). All samples of trophoblasts immediately were immersed in phosphate-buffered saline and processed with formalin for fixation. The trophoblasts were isolated using an anti-human leukocyte antigen-G antibody coupled to magnetic nanoparticles. β-human chorionic gonadotropin (hCG)-expressing cells were considered as trophoblast cells, and the detection rate calculated. DS was confirmed by fluorescence in situ hybridization (FISH). Results: The mean trophoblast detection rate using β-hCG was 78.1%, and the detection rate using FISH was 22.2%. In all cases, the trisomy of chromosome 21 was identified. Conclusion: Trophoblast can be obtained from the five weeks of gestation and has a high detection rate, so it is noted that it can replace the current prenatal genetic test. To realize the clinical application as a prenatal genetic test, we will need additional efforts to identify trisomy 21 as well as other chromosomal abnormalities in future large-scale studies.