• Korean Journal of Veterinary Research
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• v.23 no.2
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• pp.165-172
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• 1983

The distribution of slide coagulase-negative staphylococci isolated from bovine mastitic milk samples was investigated mainly according to Kloos and Schleifer's classification scheme, and toxigenic and enzymatic characteristics of these strains were also examined. One-hundred-and-twenty-one strains of coagulase-negative staphylococci isolated were classified into 8 species. Of these species, Staphylococcus epidermidis, Staph. xylosus, Staph. haemolyticus and Staph. simulans were more frequently found in bovine mastitic milk samples, and toxin and enzyme production of these species were observed in relatively high frequency. Staph. hyicus subsp. hyicus was isolated from the 4 quarters with clinical mastitis included in this investigation. By the use of Baird-Parker and Pelzer's classification system, 44.6% and 18.2% of the strains could not be classified in any subgroup, respectively.

• Journal of Plant Biotechnology
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• v.7 no.3
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• pp.187-193
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• 2005

A protocol was developed for somatic embryogenesis with 100% induction rate from immature zygotic embryo axes of peanut (Arachis hypogaea L. cv. DRG-12) cultured on MS medium containing $18.09\;{\mu}M$ 2,4-D. The frequency of somatic embryogenesis (31.7%) as well as the number of somatic embryos induced per explant (6.6) decreased when the concentration of 2,4-D was increased to $72.4\;{\mu}M$. Morphologically abnormal somatic embryos were observed at a frequency of 43.3% on MS medium containing $72.4\;{\mu}M$ 2,4-D. Somatic embryos isolated from 30-day-old cultures of immature zygotic embryo axes exhibited precocious germination with varied responses when placed on MS basal medium with 3% sucrose. Maximum shoot induction (80.0%) was observed from somatic embryos isolated from 60-day-old cultures of immature zygotic embryo axes when placed as a clump rather than individually on MS medium supplemented with $26.63\;{\mu}M$ BA and $0.54\;{\mu}M$ NAA. Shoots developed from somatic embryos rooted with higher frequency (93.3%) on Blaydes' medium containing $5.4\;{\mu}M$ NAA.

• Archives of Pharmacal Research
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• v.27 no.6
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• pp.646-652
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• 2004

The objective of this study was to determine the acute effect of trimetazidine (TMZ) on the pre-fatigue, fatigue and post-fatigue contractile characteristics and tension-frequency relationships of isolated rat diaphragm muscle. Muscle strips were taken from the ventral-costal aspects of the diaphragm muscle of rats killed by decapitation. The muscle strips were suspended in organ baths containing Krebs solution, with a gas mixture of 95% $O_2$ and 5% $CO_2$ at $37^{\circ}C$ and pH 7.35-7.45. After determining the thermoregulation and optimum muscle length the muscles were subjected to direct supramaximal stimulation with 0.05 Hz frequency square pulses for periods of 0.5 msec to obtain control values. After adding $5{\times}10^{-6}{\;}and{\;}5{\times}10^{-5}$ M trimetazidine solution to the respective bath media, the contractile parameters of the muscles were recorded. The contractile parameters were also recorded for both the trimetazidine and tri-metazidine-free media after application of the high frequency fatigue protocols. Later, the tension-frequency relationship was determined by applying stimulating pulses of 10, 20, 50 and 100 Hz to the muscle strips. Whilst the twitch tension obtained from the $5{\times}10^{-6}{\;}and{\;}5{\times}10^{-5}$ M trimetazidine media showed numerical increases compared to that of the controls, these were not statistically significant (p>0.05). The contraction time exhibited a dose dependent increase (p<0.001), whilst the contraction and relaxation rates did not differ significantly. The isometric contraction forces obtained with the different stimulating frequencies showed a significant increase in the tetanic contraction only at 100 Hz (p<0.05). A comparison of the pre- and post-fatigue twitch tensions in the trimetazidine media showed the post- fatigue twitch tensions to be significantly higher than those of the pre-fatigue contraction forces (p<0.05). In the $5{\times}10^{-6}{\;}and{\;}5{\times}10^{-5}$ M trimetazidine media the increases in the post-fatigue contraction force were 22 and 30%, respectively. These results demonstrated that in isolated rat diaphragm muscle, TMZ significantly limited the mechanical performance decrease during fatigue. It is our opinion that trimetazidine contributed to the observed fatigue tolerance by eliminating the factors of fatigue, due to preservation of intracellular calcium homeostasis, provision of the ATP energy levels needed by ATPase dependent pumps and especially by keeping the intracellular pH within cer-tain limits.

• Journal of Power Electronics
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• v.16 no.3
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• pp.861-871
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• 2016

With high penetration of renewable energies, power electronic transformers (PETs) will be one of the most important infrastructures in the future power delivery and management system. In this study, an isolated bidirectional modular multilevel DC/DC converter is proposed for PET applications. A modular multilevel structure is adopted as switching valves to sustain medium voltages to achieve modular design and high reliability. Only one high-frequency transformer is used in the proposed converter, which significantly simplifies the circuit and galvanic insulation design. A dual-phase-shift modulation strategy is proposed to regulate the output power and achieve a simple voltage balancing control. A down-scaled (2 kW/20 kHz) prototype is constructed to demonstrate the proposed converter and verify the control strategy. The experimental results comply with the theoretical analysis well, with the highest power efficiency reaching 97.6%.

• Mycobiology
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• v.50 no.6
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• pp.408-419
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• 2022

Filamentous fungi that could be classified into Aspergillus flavus/oryzae were isolated from traditionally fermented meju commercially available in Korea. The samples were analyzed for aflatoxin B1 and ochratoxin A contamination by HPLC; however, no toxin was detected. In addition, fungal and bacterial metagenomic sequencing were performed to analyze the microbial distribution in the samples. The results revealed that the distribution and abundance of fungi and bacteria differed considerably depending on the production regions and fermentation conditions of the meju samples. Through morphological analysis, ITS region sequencing, and assessment of the aflatoxin-producing ability, a total of 32 A. flavus/oryzae strains were identified. PCR analysis of six regions with a high mutation frequency in the aflatoxin gene cluster (AGC) revealed a total of six types of AGC breaking point patterns. The A. flavus/oryzae strains did not exhibit the high amylase activity detected in the commercial yellow koji strain (starter mold). However, their peptidase and lipase activities were generally higher than that of the koji isolates. We verified the safety of the traditionally fermented meju samples by analyzing the AGC breaking point pattern and the enzyme activities of A. flavus/oryzae strains isolated from the samples. The isolated strains could possibly be used as starter molds for soybean fermentation.

• The Korean Journal of Mycology
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• v.42 no.1
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• pp.69-73
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• 2014

Black molds were occurred on silicon of washbasin in a bathroom in apartment. Blocks of silicon where the fungi grew, were placed on MEA, DG18, DRBC, PDA+PS media, and 18 strains of fungi were isolated. They were identified as Phoma herbarum, Peyronellaea calorpreferens, Cladosporim halotolerans based on morphological and molecular methods. Ph. herbarum and Pe. calorpreferens were isolated with high frequency, but C. halotolerans was isolated with low frequency. The fungal strains were deposited in Korean Agricultural Culture Collection (KACC), and they shall be used for further related researches.

• Mycobiology
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• v.45 no.4
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• pp.312-317
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• 2017

Orchidaceous plants have symbiotic relationships with endophytic fungi, including mycorrhizal fungi, which play important roles in the seed germination and growth of the host plants. In this study, endophytic fungal communities isolated from the roots of Cephalanthera longibracteata collected from three different sites in Korea were analyzed, and it was determined whether fungal communities were preferentially correlated with the sites. The fungal isolates were identified by sequence analysis of the internal transcribed spacer regions of rDNA. In total, 30 species of endophytic fungi, including two species of mycorrhizal fungi belonging to the genus Tulasnella, were identified. Leptodontidium orchidicola showed the highest frequency and was isolated from all root samples. Species diversity and richness were not significantly different among sites. However, the community structure of the endophytic fungi significantly differed among sites, suggesting that the site characteristics affected the community composition of the endophytic fungi colonizing the roots of C. longibracteata. Our findings will aid in developing methods involving the use of symbiotic fungi for orchid conservation and restoration in native habitats.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.20 no.4
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• pp.21-28
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• 2006

In this paper, the proposed full-bridge high frequency isolated zoo voltage and zero current switching phase shifted pulse width modulation(ZVZCS PS-PWM)DC-DC converter among fuel cell generation system consist of 1.2[kW] fuel cell of Nexa Power Module, full-bridge DC-DC converter to boost the fuel cell low voltage($28{\sim}43[%]$) to 380[VDC] and a single phase full-bridge inverter is implemented to produce AC output(220[VAC], 60[Hz]). A tapped inductor filter with freewheeling diode is newly implemented in the output filter of the proposed full-bridge high frequency isolated ZVZCS PS-PWM DC-DC converter to suppress circulating current under the wide output voltage regulation range, thus to eliminate the switching and transformer turn-on/off over-short voltage or transient phenomena. Besides the efficiency of $93{\sim}97[%]$ is obtained over the wide output voltage regulation ranges and load variations.

• Journal of IKEEE
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• v.12 no.4
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• pp.239-245
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• 2008

In this paper, a dc-dc power converter scheme with the FPGA based technology is proposed to apply for solar power system which has many features such as the good waveform, high efficiency, low switching losses, and low acoustic noises. The circuit configuration is designed by the conventional control type converter circuit using the isolated dc power supply. This new scheme can be more widely used for industrial power conversion system and many other purposes. Also, I proposed an efficient photovoltaic power interface circuit incorporated with a FPGA based DC-DC converter and a sine-pwm control method full-bridge inverter. The FPGA based DC-DC converter operates at high switching frequency to make the output current a sine wave, whereas the full-bridge inverter operates at low switching frequency which is determined by the ac frequency. As a result, we can get a 1.72% low THD in present state using linear control method. Moreover, we can use stepping control method, we can obtain the switching losses by Sp measured as 0.53W. This paper presents the design of a single-phase photovoltaic inverter model and the simulation of its performance.

• Proceedings of the KIEE Conference
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• 2004.10a
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• pp.215-217
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• 2004

This paper shows the analysis of the inductive power transfer system in conjunction with series resonant converter operating variable high frequency. Of particular interest is the sensitivity of the complete system to variations in operational frequency and parameters. In inductive power transfer system, electrical power is transferred from a primary winding in the form of a coil or track, to one or more isolated pick-up coils that may relative to the primary. The ability to transmit power without contact enables high reliability and easy maintenance that allows inductive power transfer system to be implemented in hostile environments. This technology has found application in many fields such as electric vehicles, PRT(Personal Rapid Transit) etc. The coupling between the primary and secondary is then presented to include the effects of parameter and operational frequency variation.