• Journal of Microbiology and Biotechnology
• /
• v.9 no.2
• /
• pp.230-233
• /
• 1999

A plasmid vector was constructed for the expression and secretion of Bacillus macerans cyclodextrin glucanotransferase (CGTase) in Bacillus subtilis. The vector, pUBACGT, was composed of the ribosome-binding sequence, signal sequence, and cgt gene from B. macerans under the control of amyR2, the promoter of amyE gene coding for $\alpha$-amylase from B. subtilis var. natto. Bacillus subtilis LKS88, a mutant strain lacking genes for an amylase and two proteases, was used as a host for the transformation of the plasmid vector. The transformants were selected on kanamycin-containing Luria-Bertani plates. The starch hydrolyzing activity was observed on the starch-containing plates by the iodine method and cyclodextrin-forming activity was detected in the culture medium. A SDS-PAGE analysis showed that most of the expressed CGTase in the recombinant B. subtilis was secreted into the medium at a high expression level.

• Journal of the Korean Society of Systems Engineering
• /
• v.8 no.2
• /
• pp.19-25
• /
• 2012

As modern weapon systems have evolved into embedded software systems, the capability to develop, install, and maintain high-quality software products is becoming increasingly valuable in today's competitive aerospace and defense industry. It is desirable, especially for government-contracted projects whose end-users are military personnel, that specific types of software quality elements are assured early during product development; namely, functionality and usability. Motivated by this need to approach improving software quality from a user's perspective, this paper presents a case study that analyzes system-level software integration testing results for a weapon system indigenously developed in the Republic of Korea. Quantitative data, such as software defect ratios and configuration change rates, are computed to observe meaningful trends with respect to functionality - represented by tests designed to verify the system's electrical signal interfaces - and usability, which is evaluated in terms of how often the initial human-computer interface had to be re-designed. Insights gained from these exercises are subsequently presented as a set of "lessons learned" to take into account when developing new software items for complex weapon systems.

• 한국HCI학회:학술대회논문집
• /
• 2007.02a
• /
• pp.268-273
• /
• 2007

In this paper, we present effective methods for automatic music recommendation system which automatically recommend music by signal processing technology. Conventional music recommendation system use users’ music downloading pattern, but the method does not consider acoustic characteristics of music. Sometimes, similarities between music are used to find similar music for recommendation in some method. However, the feature used for calculating similarities is not highly related to music characteristics at the system. Thus, our proposed method use high-level music characteristics such as rhythm pattern, timbre characteristics, and the lyrics. In addition, our proposed method store features of music, which individuals queried, to recommend music based on individual taste. Experiments show the proposed method find similar music more effectively than a conventional method. The experimental results also show that the proposed method could be used for real-time application since the processing time for calculating similarities between music, and recommending music are fast enough to be applicable for commercial purpose.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.21 no.12
• /
• pp.3215-3226
• /
• 1996

In this paper a K-band harmonic oscillator competitive to ordinary Push-Push type oscillators is introduced. This oscillator is composed of two-X-band dielectric resonance circuits. To favor its harmonic generation, the load effect and the bias effect are studied to allow the maximum harmonic distortion. As results, the dielectric resonated load and the class A bias are used for the 2nd harmonic generation. analytical study for modelling of voltage controlled dielectric resonator is carried out with theoretical background. The performance of the circuit is evaluated by simulation using harmonic balanced method. The novel structure has ont only a voltage tuning circuit but also an output port at fundamental frequency as the function of prescaler for phase lockede loop application on the just single oscillation structure. In experimentation, the output freqneyc of the 2nd harmonic signal is 20.5GHz and the maximum power level of output is +5.5dBm without additional post amplifiers. the harmonic oscillator exhibits -30dBc of high fundamental frequency rejection without added extra filters. The phase noise of -90dBc/Hz at 100kHz off-carrier has been achieved under free running condition, that satisfies phase noise requirement of IESS 308. The proposed oscillator may be utilized as the clean and stable fixed local oscillator in Transmit Block Upconvertor(TBU) or Low oise Block downconvertor(LNB) for K/Ka-band digital communications and satellite broadcastings.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
• /
• 2004.07b
• /
• pp.1222-1225
• /
• 2004

This paper presents multibit Sigma-Delta ADC using noise-shaped dynamic element matching(DEM). 5-bit flash ADC for multibit quantization in Sigma Delta modulator offers the following advantages such as lower quantization noise, more accurate white-noise level and more stability over single quantization. For the feedback paths consisting of DAC, the DAC element should have a high matching requirement in order to maintain the linearity performance which can be obtained by the modulator with a multibit quantizer. The DEM algorithm is implemented in such a way as to minimize additional delay within the feedback loop of the modulator Using this algorithm, distortion spectra from DAC linearity errors are shaped. Sigma Delta ADC achieves 82dB signal to noise ratio over 615H7z bandwidth, and 62mW power dissipation at a sampling frequency of 19.6MHz. This Sigma Delta ADC is designed to use 0.25um CMOS technology with 2.5V supply voltage and verified by HSPICE simulation.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
• /
• v.19 no.2
• /
• pp.174-180
• /
• 2008

The pulsed amplifier which switches the main supply voltage of RF amplifier according to input pulse signal has good efficiency and low noise level between pulses. And it has simple structure because it doesn't need a pulse modulator at input port. The pulsed amplifier using the conventional switching circuit has slow fall time compared to rise time. We proposed the novel switching circuit for improving the fall time of pulsed amplifier The proposed switching circuit is implemented by replacing FET of conventional circuit with BJT. As a result of appling this circuit to RF pulsed amplifier, the rise and fall time are 5.7 ns and 21.9 ns at 27 dBm output power, respectively.

• The Plant Pathology Journal
• /
• v.16 no.1
• /
• pp.9-18
• /
• 2000

Using differential display techniques, a new acidic pathogenesis-related (PR) protein-1 cDNA (GMPRla) gene was isolated from a cDNA library of soybean (Glycinemax L.Merr, cultivar Jangyup) hypocotyls infected by Phytophthora sojae f. sp. glycines. The 741 bp of fulllength GMPRla clone contains an open reading frame of 525 nucleotides encoding 174 amino acid residues (pI 4.23) with a putative signal peptide of 27 amino acids in the N-terminus. Predicted molecular weight of the protein is 18,767 Da. The deduced amino acid sequence of GMPRla has a high level of identity with PR-1 proteins from Brassica napus, Nicotiana tabacum, and Sambucus nigra. The GMPRla mRNA was more strongly expressed in the incompatible than the compatible interaction. The transcript accumulation was induced in the soybbean hypocotyls by treatment with ethephon or DL-$\beta$-amino-n-butyric acid, but not by wounding. In situ hybridization data showed that GMPRIa mRNAs were usually localized in the vascular bundle of hypocotyl tissues, especially phloem tissue. Differences between compatible and incompatible interactions in the timing of GMPRla mRNA accumulation were remarkable, but the spatial distribution of GMPRla mRNA was similar in both interactions. However, more GMPRla mRNA was accumulated in soybean hypocotyls at 6 and 24 h after inoculation.

• IEMEK Journal of Embedded Systems and Applications
• /
• v.11 no.6
• /
• pp.379-392
• /
• 2016

This paper presents the design and simulation of a laser power beaming (LPB) system for an electric vehicle that establishes an optimal power transmission path based on the received signal strength. The LPB system is possible to transfer power from multiple transmitters to a single receiver according to the characteristics of the laser and the solar panel. When the laser beams of multiple transmitters aim at a solar panel at the same time, the received power is the sum of all energy at a solar panel. Our proposed LPB system consists of multiple transmitters and multiple receivers. The transmitter sends its power characteristics as optically coded pulses with a class 1 laser beam and powers as a high-intensity laser beam. By using the attenuated power level, the receiver can estimate the maximum receivable powers from the transmitters and select optimal transmitters. Throughout the simulation, we verified the possibility that different LPB receivers were achieved their required power by the optimal allocation of the transmitter among the various transmitters.

• KSII Transactions on Internet and Information Systems (TIIS)
• /
• v.8 no.7
• /
• pp.2464-2479
• /
• 2014

Multi-view video coding is an international encoding standard that attains good performance by fully utilizing temporal and inter-view correlations. However, it suffers from high computational complexity. This paper presents a fast encoder design to reduce the level of complexity. First, when the temporal correlation of a group of pictures is sufficiently strong, macroblock-based inter-view prediction is not employed for the non-anchor pictures of B-views. Second, when the disparity between two adjacent views is above some threshold, frame-based inter-view prediction is disabled. Third, inter-view prediction is not performed on boundary macroblocks in the auxiliary views, because the references for these blocks may not exist in neighboring views. Fourth, finer partitions of inter-view prediction are cancelled for macroblocks in static image areas. Finally, when estimating the disparity of a macroblock, the search range is adjusted according to the mode size distribution of the neighboring view. Compared with reference software, these techniques produce an average time reduction of 83.65%, while the bit-rate increase and peak signal-to-noise ratio loss are less than 0.54% and 0.05dB, respectively.

• The Plant Pathology Journal
• /
• v.21 no.3
• /
• pp.283-287
• /
• 2005

Since the discovery of generation of $O_2^-$ in plant, many evidence for the oxidative burst (OXB) has been accumulated in various combinations of plant and pathogen or elicitor systems. $O_2^-$ generating system responsible for the OXB was coupled with oxidation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) in microsomal fraction isolated from sliced aged potato tuber slices which were treated by hyphal wall components elicitor from Phytophthora infestans (HWC). We developed new assay method for quantitative measurement of oxygen radical $O_2^-$ by using electron spin resonance (ESR) analysis during elicitor­induced OXB on the surface of plant tissues. The ESR analysis using an $O_2^-$ trapper, Tiron (1,2-dihydroxy-3,5­benzenedisulfonic acid), provided a convenient assay for detecting only $O_2^-$ during elicitor-induced OXB producing various active oxygen species (AOS) on plant tissue surface. Tiron was oxidized to Tiron semiquinon radical by $O_2^-$. Quantity of the radical signal was measured by specific spectra on ESR spectroscopy. The level of $O_2^-$ was high in from surface of potato tuber tissue treated with hyphal cell wall elicitor (HWC) from Phytophthora infestans. There was no secondary OXB induction by $H_2O_2$ treatment in plant.