The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
권호 표지
DOI QR코드

DOI QR Code

Convenient Assay of O2- Generated on Potato Tuber Tissue Slices Treated with Fungal Elicitor by Electron Spin Resonance - No Secondary Oxidative Burst Induction by H2O2 Treatment

  • Park, Hae-Jun (Bio Dreams Co. Ltd.,School of Bioagriculture Sciences Nagoya University) ;
  • Doke, Noriyuki (School of Bioagriculture Sciences Nagoya University)
  • Published : 2005.09.01
Download PDF
⟨ Previous Next ⟩

Abstract

Since the discovery of generation of $O_2^-$ in plant, many evidence for the oxidative burst (OXB) has been accumulated in various combinations of plant and pathogen or elicitor systems. $O_2^-$ generating system responsible for the OXB was coupled with oxidation of reduced nicotinamide adenine dinucleotide phosphate (NADPH) in microsomal fraction isolated from sliced aged potato tuber slices which were treated by hyphal wall components elicitor from Phytophthora infestans (HWC). We developed new assay method for quantitative measurement of oxygen radical $O_2^-$ by using electron spin resonance (ESR) analysis during elicitor­induced OXB on the surface of plant tissues. The ESR analysis using an $O_2^-$ trapper, Tiron (1,2-dihydroxy-3,5­benzenedisulfonic acid), provided a convenient assay for detecting only $O_2^-$ during elicitor-induced OXB producing various active oxygen species (AOS) on plant tissue surface. Tiron was oxidized to Tiron semiquinon radical by $O_2^-$. Quantity of the radical signal was measured by specific spectra on ESR spectroscopy. The level of $O_2^-$ was high in from surface of potato tuber tissue treated with hyphal cell wall elicitor (HWC) from Phytophthora infestans. There was no secondary OXB induction by $H_2O_2$ treatment in plant.

Keywords

References

  1. Auh, C.-K. and Murphy, M. 1995. Plasma membrane redox enzyme is involved in the synthesis of $O_2^-\;and\;H_2O_2$ by Phytophthora Elicitor-stimulated rose cells. Plant Physiol. 107: 1241-1247 https://doi.org/10.1104/pp.107.4.1241
  2. Doke, N. 1983a. Involvement of superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race of Phytophthora infestans and to the hyphaI wall components. Physiol. Plant Pathol. 23:345-357 https://doi.org/10.1016/0048-4059(83)90019-X
  3. Doke, N. 1983b. Generation of superoxide anion by potato tuber protoplasts upon the hypersensitive response to hyphal wall components of Phytophthora infestans and specific inhibition of the reaction by suppressors of hypersensitivity. Physiol. Plant Pathol. 23:359-367 https://doi.org/10.1016/0048-4059(83)90020-6
  4. Doke, N. 1985. NADPH-dependent $O_2^-$- generation in membrane fraction isolated from wounded potato tubers inoculated with Phytophthora infestans. Physiol. Plant Pathol. 27:311-322 https://doi.org/10.1016/0048-4059(85)90044-X
  5. Doke, N. and Tomiyama, K. 1980. Effect of hyphaI with components from Phytophthora infestans on protoplasts of potato tuber tissues. Physiol. Plant Pathol. 16:169-176 https://doi.org/10.1016/0048-4059(80)90031-4
  6. Doke, N. and Ohashi, Y. 1988. Involvementof an $O_2^-$- generating system in the induction of necrotic lesions on tobacco leaves infected with tobacco mosaic virus. Physiol. Mol. Plant Pathol. 32:163-175 https://doi.org/10.1016/S0885-5765(88)80013-4
  7. Doke, N. and Miura, Y. 1995. In vitro activation of NADPH-dependent $O_2^-$- generating system in a plasma membrane-rich fraction of potato tuber tissues by treatment with an elicitor from Phytophthora infestans or with digitonin. Physiol. Mol. Plant Pathol. 46:17-28 https://doi.org/10.1006/pmpp.1995.1002
  8. Doke, N., Miura, Y., Sanchez, L. M., Park, H.-J., Noritake, T., Yoshioka, H. and Kawakita, K. 1996.The oxidative burst protects plants against pathogen attack: mechanism and role as an emergency signal for plant bio-defence. Gene 179:45-51 https://doi.org/10.1016/S0378-1119(96)00423-4
  9. Hancock, J. and Jones, O. 1987. The inhibition by diphenylene iodonium and its analogues of superoxide generation by macrophage. Biochem. J. 242:103-107 https://doi.org/10.1042/bj2420103
  10. Jabs, T., Tschope, M., Colling, C., Hahlbrock, K. and Scheel, D. 1997. Elicitor-stimulated ion fluxes and $O_2$- from the oxidative burst are essential components in triggering defense gene activation and phytoalexin synthesis in parsley. Proc. Natl. Acad. Sci. USA 94:4800-4805
  11. Mayak, S., Legge, R. L. and Thompson, J. E. 1983. Superoxide radical production by microsomal membranes from senescing carnation flowers: an effect on membrane fluidity. Phytochemistry 22:1375-1380 https://doi.org/10.1016/S0031-9422(00)84018-2
  12. Mehdy, M. C. 1994. Active oxygen species in plant defense against pathogens. Plant Physiol. 105:467-472 https://doi.org/10.1104/pp.105.2.467
  13. Miura, Y., Yoshioka, H. and Doke, N. 1995. An autophotograpic determination of the active oxygen generation in potato tuber discs during hypersensitive response to fungal infection or elicitor. Plant Sci. 105:45-52 https://doi.org/10.1016/0168-9452(94)04040-N
  14. Park, H.-J., Miura, Y., Kawakita, K., Yoshioka, H. and Doke, N. 1998. Physiological mechanisms of a sub-systemic oxidative burst triggered by elicitor-induced local oxidative burst in potato tuber slices. Plant Cell Physiol. 39:1218-1225 https://doi.org/10.1093/oxfordjournals.pcp.a029323
  15. Price, A. H., Taylor, A., Ripley, S. J., Griffiths, A., Trewavas, A. J. and Knght, M. R. 1994. Oxidative signals in tobacco increase cytosolic calcium. Plant Cell 6:1301-1310 https://doi.org/10.1105/tpc.6.9.1301
  16. Qiu, Q.-S., Liang, H.-G., Zheng, H.-J. and Chen, P. 1994. $Ca^{2+}-$ calmodulin- stimulated superoxide generation by purified plasma membrane from wheat roots. Plant Sci. 101:99-104 https://doi.org/10.1016/0168-9452(94)90245-3
  17. Rossi, F. 1986. The forming NADPH oxidase of the phagocytes: nature, mechanisms of activation and function. Biochemi. Biophysica Acta 853:65-89 https://doi.org/10.1016/0304-4173(86)90005-4

Cited by

  1. Bacterial RNAs activate innate immunity in Arabidopsis vol.209, pp.2, 2016, https://doi.org/10.1111/nph.13717
  2. Superoxide anion regulates plant growth and tuber development of potato vol.26, pp.10, 2007, https://doi.org/10.1007/s00299-007-0380-1
  3. Apoplastic hydrogen peroxide and superoxide anion exhibited different regulatory functions in salt-induced oxidative stress in wheat leaves vol.62, pp.4, 2018, https://doi.org/10.1007/s10535-018-0808-1