• Korean Journal of Plant Resources
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• v.30 no.3
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• pp.272-279
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• 2017

EP was obtained through 20% ethanol extraction of Pueraria lobata root, and the fermented form of EP, FEP, was prepared from the EP after incubating with Lactobacillus rhamnosus vitaP1. There was no significant toxicity by EP and FEP up to $1000{\mu}g/ml$ in NIH-3T3, HaCaT, and B16F10 cells. In addition to antioxidant potentials of EP and FEP determined by DPPH and ABST assays, we confirmed increase of procollagen type I and elastin synthesis by supplementation of the EP and FEP at the concentration of $50{\mu}g/ml$ using ELISA kits. The protein expression levels of matrix metalloprotease (MMP)-1, -3, and -9, those are involved in the degradation of collagen or other skin matrix proteins, were remarkably suppressed while their inhibitory protein metallopeptidase inhibitor 1 (TIMP-1) was greatly up-regulated by supplementation of the EP and FEP at a concentration of $50{\mu}g/ml$. Taken together, both EP and FEP supplementation could be involved in the suppression of the skin wrinkle formation through inhibiting degradation of collagen and stimulating the synthesis of collagen and elastin. The results showed that the anti-wrinkle potential of the EP and FEP will be a promising candidate for developing cosmeceutical compounds or products.

• The Korea Journal of Herbology
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• v.33 no.3
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• pp.11-18
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• 2018

Objectives : Saururus chinensis and Houttuynia cordata (Saururaceae) are perennial herbs using for medicinal purposes in Korea. The objectives of this study are to compare anatomical key characters between two medicinal plants and to provide fundamental information for the identification of two herbal medicines by using anatomical features. Methods : Cross-sections of root, rhizome, stem, petiole, and leaf for each species were observed in this study. Materials were analyzed through dehydration, paraffin embedding and micro-sectioning, and double staining with Safranin O and Fast-Green FCF. Observations of permanent preparation were conducted using light microscope. Results : S. chinensis and H. cordata were distinguished with anatomical differentiations; Idioblasts with essential oil were scattered in the parenchyma cell of cortex, pith, and phloem of S. chinensis, on the other hand, in H. cordata, idioblasts were distributed ring-shaped in the cortex of the root. S. chinensis had two cycles of vascular bundles in the stem while H. cordata had one cycle. Hypodermis layer was conspicuous in a stem of H. cordata, crystals were observed the only parenchyma in a stem of S. chinensis, and epidermal oil cells were developed in the epidermis of H. cordata. S. chinensis had air cavity at the cortex and pith of the stem. The shape of cross-section was polygonal in the stem of S. chinensis and was circular in the stem of H. cordata. Conclusions : We investigated anatomical study of Korean S. chinensis and H. cordata. To identify two herbal medicines, we considered main anatomical features and provided identification key here.

• The Korea Journal of Herbology
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• v.35 no.2
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• pp.39-46
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• 2020

Objective: In this study, we analyzed the trend of patents registered prior to October 4, 2019, to understand the industrial trends in insect-derived medicinal materials used for the treatment of skin diseases. Methods: Using the WIPSON database, we collected information regarding the patents related to insect-derived materials for treating various skin diseases. Results: The patents registered prior to October 4, 2019, from Korea, Japan, the USA, China, and the EU, along with those registered under PCT were selected. There were 195 patents related to the use of insect-derived medicinal materials in treating various skin diseases such as psoriasis, inflammatory skin diseases, eczema, pruritus, and atopic dermatitis. China is mostly superior in total number of registered patents compared with the other countries. Korea was the major patent technology-holder for atopic dermatitis, but China dominated in the remaining categories of skin diseases. Upon first patent registration in 1992, there had been a continual increase in the number of patents. Especially, patents related to eczema, psoriasis, inflammatory skin disease were markedly increased. Most frequently used insect-derived medicinal materials was Scolopendra, Cicadidae Periostracum, Scorpions, Cantharides, and Batryticatus Bombyx. The insect-derived medicinal materials were generally used as a combined preparation with other medicinal materials in patents. Conclusion: This study could help to establish the basis for future research and development related treating skin diseases using insect-derived medicinal materials. In order to provide sufficient data, further study including analysis of rejected patents is needed.

• The Journal of Internal Korean Medicine
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• v.29 no.3
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• pp.716-729
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• 2008

Objectives : We designed this study to evaluate the effectiveness of Chungin-troche(CIT) preparation as an adjunctive medicine to a herbal extract of Chungsangboha-tanggagambang(CSBH). Methods : A paralled, comparative study of CSBH+CIT and CSBH was conducted on 42 patients of chronic cough who attended Division of Allergy, Immune & Respiratory System, Department of Internal Medicine, College of Oriental Medicine, Kyung Hee University between December, 2007 and June, 2008. The treatment in each group lasted for two weeks. Patients were asked to fill in the Leicester Cough Questionnaire (LCQ) before and after administration of each treatment legimen. Results : 42 patients completed the trial and no adverse effects were reported. Both CSBH +CIT group and CSBH group showed statistical significant improvement in total LCQ score after 2 weeks from baseline. Social domain score and total score in patients taking CSBH+CIT showed a statistical significant increase compared to those scores in CSBH patients. Conclusion : CSBH+CIT compositive preparation and CSBH separate preparation appeared to have some benefits in the treatment of chronic cough. However, additional CIT preparation to CSBH can improve social domain in LCQ of chronic cough patients.

• Herbal Formula Science
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• v.15 no.2
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• pp.79-87
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• 2007

This study was investigated to make sure the range of the Colla Corii Asini treatment. the nature of disease and the pathology of it in D ongeuyboga. The following conclusion were reached through invetigations on the prescriptions that use the Colla Corii Asini as a ingredient. 1. The Colla Corii Asini was used a internal disease more than a surgical disease in the dongeuybogam. 2. The Colla Corii Asini was used lung and large intestine disease in abundance and especially anhidrosis. cough and dysentery. 3. The Colla Corii Asini was applied much more disease about blood in the dongeuybogam, but some was used almost bleeding disease. one was used blood deficiency. 4. The processed Colla Corii Asini was used more than the Colla Corii Asini in the dongeuybogam, because of convenience of preparation and ease of intestinla drug absorption. 5. The Colla Corii Asini was used more useful dysentery than tranquilizeing mind in cooperation with Rhizoma Coptidis in the dongeuybogam. 6. Instead of the Colla Corii Asini, we can apply the Colla Comus Cervi or they are mutual reinforcement.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.30 no.3
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• pp.155-165
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• 2017

Objectives : The purpose of this study is to analyze the trends of domestic experimental research of external treatment in Korean medicine and use it as a basic data to expand the therapeutic field of Korean medicine. Methods : In December 2016, two authors searched for experimental studies of external treatment in Korean medicine researches in 6 Korean databases. Results : Among the 379 researches that were initially searched, two authors reviewed 190 researches, excluding duplicate researches, and finally 11 researches were selected for the study. The target disease was confined to the skin and mucosa, and the most of selected Korean medicine was based on the literature. Conclusions : Considering the range of application of the external treatment described in the literature and the domestic market for external medicine, it is expected that the development of various external medicine using herbal medicine will be expected in the future.

• Journal of Radiation Industry
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• v.5 no.2
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• pp.113-118
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• 2011

Hydrogels from a mixture of poly(N-vinylpyrrolidone) (PVP), ${\kappa}$-carrageenan and microorganism fermentation celluose were prepared by $^{60}Co$ gamma-ray irradiation. PVP and ${\kappa}$-carrageenan were mixed with the different ratios. Microorganism fermentation celluose were added to the mixture of PVP and ${\kappa}$-carrageenan to evaluate the effect of microorganism fermentation celluose on the gel strength. The gel strength of the hydrogel was evaluated for application of a wound dressing. The results showed that gelation and gel strength were increased with increasing the content of the microorganism fermentation celluose.

• Journal of Radiation Industry
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• v.4 no.4
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• pp.365-372
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• 2010

Irradiation has been recognized as a highly suitable tool to aid in the formation of hydrogel. The radiation process has various advantage, such as easy process control and the lack of necessity for initiators and crosslinker. In this study, the hydrogel containing the citrus fermentation gel for the wound healing were successfully synthesized. The strength of hydrogel was increased as a function of a increasing the concentration of citrus and the irradiation doses. In addition, this hydrogel have been evaluated by the cytotoxicity and animal experiment.

• Journal of Applied Biological Chemistry
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• v.66
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• pp.81-89
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• 2023

The purpose of this study is to demonstrate the potential enhancement of the flavonoid contents from Agastache rugosa, which can be obtained as raw materials for functional products in the food medicine industry by identifying important factors for efficient preparation to save costs and time in terms of economic factors. For this reason, response surface methodology using Box-Behnken design was used to optimize the extraction conditions for the maximum yield of seven major compounds from A. rugosa. The optimum conditions were obtained with an ethanol concentration of 60.0%, a temperature of 50 ℃, and an extraction time of 33.6 min, meaning that the regression analysis fits the experimental data well. Under these conditions, the seven major compounds 1-7 had observed values of 2.169, 2.135, 0.697, 2.485, 0.105, 1.247, and 0.551%, respectively. These results show that the observed values are in good agreement with the predicted values in the regression model. This process for optimization study exhibited a basic protocol for obtaining stable ingredients from A. rugosa that are appropriate for the development of effective functional products.

• The Korean Journal of Pesticide Science
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• v.16 no.1
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• pp.28-34
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• 2012

We analysed 149 samples of Korean traditional herbal tea materials. The 156 pesticides were analyzed by GC/ECD and NPD, detected pesticides were confirmed by GC-TOF/MS. Sample preparation was performed bv multi-residue analysis method of multiclass pesticides of the Korea Food Code. The residual pesticides were detected in 22 samples(14.8%), the highest detection frequency samples are lycium and jujube. Detected pesticides in Korean traditional herbal tea materials were chlorpyrifos (5 samples), chlorothalonil (3 samples), cypermethrin (3 samples), hexaconazol (3 samples) and cyhalothrin(3 samples). The pesticide types detected in Korean traditional herbal tea materials were organophosphorus(29.2%), pyrethroids(16.7%), organochlorines (12.5%) and triazoles(12.5%). The 5 samples(lycium, jujube, chrysanthemum, balloon-flower, milk vetch root) were detected pesticides below MRLs, 2 samples(cornus fruit, cnidium) were detected pesticides unnotificated MRLs.