• 한국약용작물학회지
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• 제24권3호
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• pp.183-190
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• 2016

Background: This study determined the effects of mulching, an environment friendly organic cultivation method, on antioxidant compound contents and growth in Codonopsis lanceolata, commonly known as Deodeok. Methods and Results: C. lanceolata was treated by mulching with several different methods (a non-woven fabric, biodegradable film, or rice husks) and also treated with hand weeding. A non-treatment plot was used as a control. The growth and levels of weed control in C. lanceolata were better in plants cultivated under mulching treatments (non-woven fabric, biodegradable film, and rice husks) than in those under non-mulching treatments (hand weeding and non-treatment). The contents of antioxidant compounds, such as total flavonoids, phenolics, and anthocyanins, were highest under the biodegradable film treatment, followed by the non-woven fabric treatment, rice husks treatment hand weeding, and non-treatment. There were identifiable differences in DPPH and ABTS activity in comparison to antioxidant compound content by solvent fractions. Mulching treatments resulted in higher DPPH scavenging activity in water and ethyl ether fractions and ABTS scavenging activity in n-butanol fractions than in other fractions, as opposed to hand weeding and non-treatment groups, although total activity of DPPH and ABTS did not increase with mulching treatments. Conclusions: Mulching C. lanceolata with biodegradable film and non-woven fabric is an effective method for improving plant growth and inhibiting the occurrence of weeds as well as for increasing antioxidant compound content and altering antioxidant activity.

• Journal of Microbiology and Biotechnology
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• 제26권11호
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• pp.2012-2018
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• 2016

Coxsackievirus B3 (CVB3) is the main cause of acute myocarditis and dilated cardiomyopathy. Plant extracts are considered as useful materials to develop new antiviral drugs. We had previously selected candidate plant extracts, which showed anti-inflammatory effects. We examined the antiviral effects by using a HeLa cell survival assay. Among these extracts, we chose the Amomi Cardamomi (Amomi) extract, which showed strong antiviral effect and preserved cell survival in CVB3 infection. We investigated the mechanisms underlying the ability of Amomi extract to inhibit CVB3 infection and replication. HeLa cells were infected by CVB3 with or without Amomi extract. Erk and Akt activities, and their correlation with virus replication were observed. Live virus titers in cell supernatants and viral positive- and negative-strand RNA amplification were measured. Amomi extract significantly increased HeLa cell survival in different concentrations ($100-10{\mu}g/ml$). CVB3 capsid protein VP1 expression (76%) and viral protease 2A-induced eIF4G1 cleavage (70%) were significantly decreased in Amomi extract ($100{\mu}g/ml$) treated cells. The levels of positive- (20%) and negative-strand (80%) RNA were dramatically decreased compared with the control, as revealed by reverse transcription-PCR. In addition, Amomi extract improved mice survival (51% vs 26%) and dramatically reduced heart inflammation in a CVB3-induced myocarditis mouse model. These results suggested that Amomi extract significantly inhibited Enterovirus replication and myocarditis damage. Amomi may be developed as a therapeutic drug for Enterovirus.

• 한국식품저장유통학회지
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• 제14권5호
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• pp.492-496
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• 2007

구릿대 잎의 한약소재 및 식품 영양학적 소재로 활용하기 위한 연구의 일환으로 일반성분과 환원당, 유리당, 아미노산 및 아미노산 유도체의 조성 그리고 무기질 함량을 분석하였다. 일반성분은 수분이 76.30%로 가장 높았으며 탄수화물 7.58%, 조단백질 4.01%, 조회분 7.87% 그리고 조지방은 4.23%의 비율로 함유하였다. 환원당은 1,687.10 mg/100 g이였으며, 유리당 총 함량은 57.3 mg/100 g으로 나타났다. 구성아미노산은 139.25 mg/100 g이었으며, 유리아미노산 215.99 mg/100 g으로 alanine이 61.52 mg/100g으로 가장 많았으며, 아미노산 유도체는 101.39mg/100 g으로 ${\gamma}-aminoisobutyric$ acid가 78.26 mg/100 g으로 가장 높았다. 무기질은 K가 2,135.03 mg/100 g으로 가장 높았으며, Cu(916.47 mg/ 100 g), Mg(39.35 mg/100 g), Me(2.97 mg/100 g) 등이 분석되었다.

• 대한본초학회지
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• 제32권1호
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• pp.75-81
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• 2017

Objectives : The present study was designed to investigate effects of mixed extracts (9:1, v/v) of Hwangryunhaedoktang, Chamaecyparis obtusa essential oil. We evaluated the antimicrobial and antioxidant activity by manufacturing mixed extracts as the materials for functional medicinal herb cosmetics. Methods : We performed antimicrobial were tested microbes (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Candida albicans, Propionibacterium acnes) by disc diffusion method and measure clear zone. Antioxidant activities were measured by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity. Results : Antimicrobial activities of mixed extracts against S. epidermideis, P. acnes, S. aureus, E. coli, and Candida. A were $10.9{\pm}3.0mm$, $16.9{\pm}2.0mm$, $9.2{\pm}2.0mm$, $10.3{\pm}1.0mm$, $11.8{\pm}3.0mm$ respectively. The Chamaecyparis obtusa essential oil had the highest antimicrobial activities against S. epidermideis, P. acnes, S. aureus, E. coli, and Candia. A and clear zone of microbes ware $16.7{\pm}3.0mm$, $28.8{\pm}0.2mm$, $15.9{\pm}2.0mm$, $11.5{\pm}1.0mm$, $16.3{\pm}3.0mm$. Hwangryunhaedoktang extract showed antimicrobial activity but only P. acnes and S. aureus The antioxidant activities of the mixed extracts were tested through the evaluation of DPPH radical scavenging activity. The 100 % mixed extracts were found to have 90 % DPPH radical scavenging activity. The mixed extracts was presented similar antioxidant activities compared with that of ascorbic acid. As a result, A mixture extract is expected to have antimicrobial effects and free radical scavenging activity was found. Conclusions : Accordingly, It can be concluded that mixed extracts has the potential to cosmetic material.

• 한국약용작물학회지
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• 제24권2호
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• pp.93-100
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• 2016

Background: A set of logical criteria that can accurately identify and verify the cultivation region of raw materials is a critical tool for the scientific management of traditional herbal medicine. Methods and Results: Volatile compounds were obtained from 19 and 32 samples of Angelica gigas Nakai cultivated in Korea and China, respectively, by using steam distillation extraction. The metabolites were identified using GC/MS by querying against the NIST reference library. Data binning was performed to normalize the number of variables used in statistical analysis. Multivariate statistical analyses, such as Principal Component Analysis (PCA), Partial Least Squares-Discriminant Analysis (PLS-DA), and Orthogonal Partial Least Squares-Discriminant Analysis (OPLS-DA) were performed using the SIMCA-P software. Significant variables with a Variable Importance in the Projection (VIP) score higher than 1.0 as obtained through OPLS-DA and those that resulted in p-values less than 0.05 through one-way ANOVA were selected to verify the marker compounds. Among the 19 variables extracted, styrene, ${\alpha}$-pinene, and ${\beta}$-terpinene were selected as markers to indicate the origin of A. gigas. Conclusions: The statistical model developed was suitable for determination of the geographical origin of A. gigas. The cultivation regions of six Korean and eight Chinese A. gigas. samples were predicted using the established OPLS-DA model and it was confirmed that 13 of the 14 samples were accurately classified.

• 한국패션뷰티학회지
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• 제5권4호
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• pp.139-144
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• 2007

There was an increasing interest that herbal medicine and natural material extracts were proved processes of antioxidant, cosmeceutical activity and the other effects. The aim of this study was to assess the antioxidant of extraction of four kinds from Prunus armeniaca L., Reynoutria elliptica, Curcuma aromatica, Lithospermum erythrorhizon. RE (Reynoutria elliptica) and CA (Curcuma aromatica) have good electron donating ability. The water and ethanol extract of RE at a 100 ppm concentration showed over 70%, the water extract at 500 ppm concentration showed 83% and the ethanol extract at 100 ppm concentration showed 86% of CA. Xanthine oxidase inhibition activity of the water extract of LE (Lithospermum erythrorhizon) at a 1,000 ppm concentration showed over 44%, on the other hand, RE showed in all lowest effect and there was no inhibition activity of a couple more extracts. In the measurement of nitrite scavenging activity, all extracts showed highly scavenging activity. Especially the water and ethanol extract of RE showed over 99% at 500 ppm, also LE showed over 40% at 10 ppm concentration.

• 대한의료기공학회지
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• 제5권1호
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• pp.303-324
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• 2001

Medical Kigong is one of five noted methods in Hwangjenaegyeong(黃帝內經). an is an important part of Oriental medicine. Recently many people are getting interested in Medical Kigong and studying it with several problems resulting. So it is necessary to get a theory of Medical Kigong which is scientifically well-organized. For this purpose we study Dongeuibogam Naegyeong Sinhyeong(東醫寶鑑 內景 身形) in terms of the theory of Medical Kigong and draw conclusions as follows Dongeuibogam Naegyeong Sinhyeong(東醫寶鑑 內景 身形) can be said to have all the necessary factors to be a separate book itself on Medical Kigong. It is focused on the preventive medicine through various methods of Medical Kigong used from the ancient times in China, and consists of the basic theory on Medical Kigong, the principle of Kigong. treatment result. patients to treat, specific Kigong methods, general methods on improving health, herbal diets and etc. The principle of Medical Kigong in Dongeuibogam Naegyeong Sinhyeong(東醫寶鑑 內景 身形) is to preserve and cultivate jeong(精;essence), ki(氣;breath), sin(神;spirit) which are three treasures of humanbody and it is achieved through health improving methods based on kigongsamjo(氣功三調), that is, josin(調身), josik(調息), josim(調心) and daesojucheonbeop(大小周天法) of naedanbeop(內丹法) in Taoism. Dongeuibogam Naegyeong Sinhyeong(東醫寶鑑 內景 身形) accepted Kigong health improvement methods in Taoism as a practical Medical Kigong and emphasized that preventing diseases through health improvement by Kigong is better than treating diseases. It also suggested that seasonal changes and ways of life are very important for health. In short, Dongeuibogam Naegyeong Sinhyeong(東醫寶鑑 內景 身形) established the system of Medical Kigong by discribing almost all parts of it, and can be used as one of the basic materials for the study of Korean Medical Kigong.

• 한국식품과학회지
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• 제48권1호
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• pp.72-76
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• 2016

본 연구에서 국립원예특작과학원 인삼특작부 추출물은행에서 분양받은 에탄올 추출물 시료(시료번호: 4)인 선이질풀(Geranium krameri)에 대한 다양한 생리활성을 조사하여 기능성소재 응용가능성을 검토하였다. 선이질풀 추출물은 멜라노마 세포에 대하여 낮은 세포독성을 나타냈다. 세포독성이 거의 없는 농도에서 선이질풀 추출물 처리 시 산화방지 활성($IC_{50}$, $8.72{\mu}g/mL$) 및 B. subtilis, E. coli, C. albicans에 대한 항균활성이 우수하게 나타났으며 타이로시네이즈 활성저해($IC_{50}$, $456.86{\mu}g/mL$) 및 멜라닌 함량 저하($IC_{50}$, $50.35{\mu}g/mL$)를 보여주었다. 또한, B16F10 세포에서 선이질풀 추출물 농도 의존적으로 타이로시네이즈 발현이 억제되었으며, 이는 선이질풀 추출물이 직접적인 타이로시네이즈 활성저해 및 세포 내 타이로시네이즈 발현을 억제시킴으로서 멜라닌 합성을 저해하는 것으로 판단된다. 이와 같은 결과로 미루어볼 때 선이질풀 추출물은 피부미백 소재 등 피부개선 효과를 지닌 기능성 화장품에 활용하기 위한 매우 효과적인 재료가 될 수 있다고 판단된다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권6호
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• pp.2781-2785
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• 2016

Background: Matric metalloproteinase (MMP) 13 gene expression is increased in esophageal squamous cell carcinomas (ESCCs) and associated with increasing tumor invasion, lymph node involvement and decreased survival rates. Levels of the circulating enzyme may be elevated and used as a marker of tumor progression. In this study, clinical application of MMP-13 serum levels was evaluated for early detection, prediction of prognosis and survival time of ESCC patients. Materials and Methods: Serum levels of MMP13 were determined by ELISA in 66 ESCC patients prior of any treatment and 54 healthy controls for comparison with clinicopathological data through statistical analysis with Man Whitney U and Log-Rank tests. In addition, clinical value of MMP13 levels for diagnosis was evaluated by receiver operating characteristic (ROC) test. Results: The serum level of MMP-13 in patients (>250 pg/ml) was significantly higher than in the control group (<100 pg/ml) (p value=0.004). Also the results showed a significant correlation between MMP-13 serum levels with tumor stage (p value = 0.003), depth of tumor invasion (p value=0.008), involvement of lymph nodes (p value = 0.011), tumor size (p value = 0.018) and survival time. While there were no significant correlation with grade and location of tumors. ROC analysis showed that MMP-13 level is an accurate diagnostic marker especially to differentiate pre-invasive/ invasive lesions from normal controls (sensitivity and specificity: 100%). Conclusions: These findings indicate a potential clinical significance of serum MMP13 measurement for early detection and prognostic assessment in ESCC patients.

• 농업생명과학연구
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• 제46권6호
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• pp.1-8
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• 2012

본 시험은 한국 고추 육종에 이용되는 교배친의 고추맛에 관여하는 유전자의 유전가를 구명하기 위해 수행하였다. 고추맛과 원예적 특성이 상이한 '#1803'($P_1$)과 '#1532'($P_2$) 두 교배친을 교배하여 이들의 $F_1$, $F_2$ 세대를 본 시험의 공시 재료로 이용하였다. Partitioning method에 따라 고추맛은 A-a와 B-b 두 유효 유전자에 의하여 지배되는데 $F_1$ 세대는 우수 교배친 $P_1$보다 더 우수한 초우성 현상과 $F_2$ 세대는 초월 분리가 일부 나타났다. 고추맛의 유효 유전자 효과는 A-a 유전자에 의해 0.36, B-b 유전자에 의해 0.64가 증가하였다. 한국 고추맛은 유효 유전자간에서 0.94의 간섭효과와 최대 2.86의 제 2효과가 관여하여 복합적인 유전양식을 나타내었다.