• Nuclear Engineering and Technology
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• 제53권8호
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• pp.2464-2476
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• 2021

Direct-contact heat transfer of a single saturated droplet upon colliding with a heated wall in the regime of film boiling was experimentally investigated using high-resolution infrared thermometry technique. This technique provides transient local wall heat flux distributions during the entire collision period. In addition, various physical parameters relevant to the mechanistic modelling of these phenomena can be measured. The obtained results show that when single droplets dynamically collide with a heated surface during film boiling above the Leidenfrost point temperature, typically determined by droplet collision dynamics without considering thermal interactions, small spots of high heat flux due to localized wetting during the collision appear as increasing We_n. A systematic comparison revealed that existing theoretical models do not consider these observed physical phenomena and have lacks in accurately predicting the amount of direct-contact heat transfer. The necessity of developing an improved model to account for the effects of local wetting during the direct-contact heat transfer process is emphasized.

• 한국태양에너지학회 논문집
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• 제25권4호
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• pp.111-118
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• 2005

The purpose of this study is to address the progress of the heat island phenomenon and the scheme to decrease in heat island phenomenon through analysis of micro-climates according to land use and make a plan to construct wind ways. The result is: 1) Analysis of temperature and humidity at 6 spots for 24 hours showed that heat island phenomenon was considerably intense around center road of Gwangju and can be mild through making lakes and green zones. 2) Analysis of the direction and velocity of the wind at 2 spots for 24 hours showed that the direction of the wind at the center of Gwangju was SSW(South-South-West) and average velocity of the it was $1.2{\sim}1.5\;m/s$. To make the inflow of the low-temperature air current from Mt. Mudeung into the city through Gwangju river, efficient management of Gwangju riverside parks should be considered for Gwangju river itself to be wind way. 3) Analysis of mobile temperature measurement on 3 courses for 24 hours showed that the low-temperature air current of Mt. Mudeung and a micro-climate of Gwangju river can lighten thermal storage phenomena of the city in that the temperature was lowest at Gwangju riverside. These outcome is from a day term measurement. So, to figure out accurate condition of heat island phenomenon in Gwangju City, it is needed to have long term measurements and accumulation of those information.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.131-131
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• 2017

Though the Platycodon grandiflorum, has a broad range of pharmacologic properties, but the mechanisms underlying these effects remain unclear. In order to profile proteins from the nodal segment, callus, root and shoot, high throughput proteome approach was executed in the present study. Two-dimensional gels stained with CBB, a total of 84 differential expressed proteins were confirmed out of 839 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 58 differential expressed protein spots (${\geq}2-fold$) were analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. Out of 58 differential expressed protein, 32 protein spots were up-regulated such as ribulose-1,5-bisphosphate carboxylase, endoplasmic oxidoreductin-1, heat stress transcription factor A3, RNA pseudourine synthase 4, cysteine proteinase, GntR family transcriptional regulator, E3 xyloglucan 6-xylosyltransferase, while 26 differential protein spots were down-regulated such as L-ascorbate oxidase precursor, late embryogenesis abundant protein D-34, putative SCO1 protein, oxygen-evolving enhancer protein 3. However, the frequency distribution of identified proteins using iProClass databases, and assignment by function based on gene ontology revealed that the identified proteins from the explants were mainly associated with the nucleic acid binding (17%), transferase activity (14%) and ion binding (12%). Taken together, the protein profile may provide insight clues for better understanding the characteristics of proteins and its metabolic activities in various explants of this essential medicinal plant P. grandiflorum.

• 한국작물학회지
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• 제60권1호
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• pp.97-106
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• 2015

Platycodon grandiflorum, commonly known as Doraji in Korea, has a wide range of pharmacologic properties, such as reducing adiposity and hyperlipidemia, and antiatherosclerotic effects. However, the mechanisms underlying these effects remain unclear. In order to profile proteins from the nodal segment, callus, root and shoot, high throughput proteome approach was executed in the present study. Two dimensional gels stained with CBB, a total of 84 differential expressed proteins were confirmed out of 839 protein spots using image analysis by Progenesis SameSpot software. Out of total differential expressed spots, 58 differential expressed protein spots (${\geq}$ 2-fold) were analyzed using MASCOT search engine according to the similarity of sequences with previously characterized proteins along with the UniProt database. Out of 58 differential expressed protein, 32 protein spots were up-regulated such as ribulose-1,5-bisphosphate carboxylase, endoplasmic oxidoreductin-1, heat stress transcription factor A3, RNA pseudourine synthase 4, cysteine proteinase, GntR family transcriptional regulator, E3 xyloglucan 6-xylosyltransferase, while 26 differential protein spots were down-regulated such as L-ascorbate oxidase precursor, late embryogenesis abundant protein D-34, putative SCO1 protein, oxygen-evolving enhancer protein 3. However, frequency distribution of identified proteins using iProClass databases, and assignment by function based on gene ontology revealed that the identified proteins from the explants were mainly associated with the nucleic acid binding (17%), transferase activity (14%) and ion binding (12%). In that way, the exclusive protein profile may provide insight clues for better understanding the characteristics of proteins and metabolic activity in various explants of the economically important medicinal plant Platycodon grandiflorum.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회B
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• pp.2471-2476
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• 2008

Turbulent flow and heat transfer to water at supercritical pressure flowing in vertical pipes is investigated using direct numerical simulation (DNS). A conservative space-time discretization scheme for variable-density flows at low Mach numbers is adopted in the present study to treat steep variations of fluid properties at supercritical pressure just above the thermodynamic critical point. The fluid properties at these conditions are obtained using PROPATH and used in the form of tables in the simulations. The buoyancy influence induced by strong variation of density across the pseudo-critical temperature proved to play an important role in turbulent flow and heat transfer at supercritical state. Depending on the degree of buoyancy influence, turbulent heat transfer may be enhanced or significantly deteriorated, resulting in local hot spots along the heated surface.

• 대한기계학회논문집A
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• 제35권12호
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• pp.1635-1640
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• 2011

본 논문의 목적은 수치해석을 통한 자동차 와이퍼 소켓 부품 제작용 오버몰딩 금형의 냉각 시스템 설계이다. 초기 금형설계에 대한 수치해석을 수행하여 제품내 높은 온도 분포를 나타내는 열집중 영역을 도출하였다. 열집중 영역의 냉각특성 개선을 위하여 직선형 냉각수로와 체적 열 흡수부의 2 가지 냉각시스템을 고려하였다. 직선형 냉각수로의 설계를 도출하기 위하여 냉각수로의 직경과 위치가 냉각 특성과 제품 품질에 미치는 영향을 정량적으로 분석하였다. 또한 체적 열 흡수부 설계가 제품 냉각 특성과 변형에 미치는 영향을 고찰하였다. 최종적으로 체적 열 흡수부가 추가된 다중 슬라이스 오버몰딩 금형이 냉각 특성과 제품품질을 동시에 향상시킬 수 있음을 알 수 있었다.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2008년도 춘계학술대회논문집
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• pp.648-651
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• 2008

Numerical calculations are performed to simulate the film cooling effect of turbine blade tip with squealer rim. Because of high temperature of inside rim, squealer rim is damaged easily. Therefore many various cooling systems were used. The calculations are based on 100,000 Reynolds number in linear cascade model. A blade has 2% tip clearance and 8.4% rim height. The axial chord length and turning angle is 237mm, 126$^{\circ}$. Numerical calculations are performed without and with film cooling. In a film cooling in the cavity, hot spots of cavity were cooled effectively. However hot spots of suction side rim still remains. The CFD results show that the circulation flow in cavity of squealer tip affects the temperature rise of squealer rim. To maintain the blade integrity and avoid the excessive hot spot of blade, rearrangement of cooling hole is needed.

• Parasites, Hosts and Diseases
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• 제55권1호
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• pp.15-20
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• 2017

The aim of this study was to identify antigens for a vaccine or drug target to control rabbit coccidiosis. A combination of 2-dimensional electrophoresis, immunoblotting, and mass spectrometric analysis were used to identify novel antigens from the sporozoites of Eimeria stiedae. Protein spots were recognized by the sera of New Zealand rabbits infected artificially with E. stiedae. The proteins were characterized by matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF/TOF-MS) analysis in combination with bioinformatics. Approximately 868 protein spots were detected by silver-staining, and a total of 41 immunoreactive protein spots were recognized by anti-E. stiedae sera. Finally, 23 protein spots were successfully identified. The proteins such as heat shock protein 70 and aspartyl protease may have potential as immunodiagnostic or vaccine antigens. The immunoreactive proteins were found to possess a wide range of biological functions. This study is the first to report the proteins recognized by sera of infected rabbits with E. stiedae, which might be helpful in identifying potential targets for vaccine development to control rabbit coccidiosis.

• Journal of Microbiology and Biotechnology
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• 제17권6호
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• pp.934-944
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• 2007

Paenibacillus polymyxa E681 is known to be able to suppress plant diseases by producing antimicrobial compounds and to promote plant growth by producing phytohormones, and secreting diverse degrading enzymes. In spite of these capabilities, little is known regarding the flow of information from the bacterial strain to the barley roots. In an attempt to determine the flow of information from the bacterial strain to barley roots, the strain was grown in the presence and absence of barley, and two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and MALDI-TOF mass spectrometry were used. 2D-PAGE detected approximately 1,000 spots in the cell and 1,100 spots in the supernatant at a pH 4-10 gradient. Interestingly, about 80 spots from each sample showed quantitative variations. Fifty-three spots from these were analyzed by MALDI-TOF mass spectrometry and 28 proteins were identified. Most of the cytosolic proteins expressed at higher levels were found in P. polymyxa E681 cells grown in the presence of barley rather than in the absence of barley. Proteins detected at a lower level in the surpernatant of P. polymyxa E68l cells grown in the presence of barley were lipoprotein, glucose-6-phosphate 1-dehydrogenase, heat-shock protein HtpG, spermidine synthase, OrfZ, ribonuclease PH, and coenzyme PQQ synthesis protein, and flagellar hook-associated protein 2 whereas proteins detected at a higher level in the surpernatant of P. polymyxa E681 cells grown in the presence of barley included D-alanyl-D-alanine ligase A, isopentenyl-diphosphate delta-isomerase, ABC transporter ATP-binding protein Uup, lipase. Many of the proteins belonging to plant-induced stimulons are associated with biosynthetic metabolism and metabolites of proteins and transport. Some of these proteins would be expected to be induced by environmental changes resulting from the accumulation of plant-secreted substances.

• 대한기계학회논문집B
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• 제28권11호
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• pp.1302-1314
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• 2004

Turbulent heat transfer to $CO_2$ at supercritical pressure flowing in vertical tubes is investigated using direct numerical simulation (DNS). A conservative space-time discretization scheme for variable-density flows at low Mach numbers is adopted in the present study to treat steep variations of fluid properties at supercritical pressure just above the thermodynamic critical point. The fluid properties at these conditions are obtained using PROPATH and used in the form of tables in the simulations. The buoyancy influence induced by strong variation of density across the pseudo-critical temperature proved to play a major role in turbulent heat transfer at supercritical state. Depending on the degree of buoyancy influence, turbulent heat transfer may be enhanced or significantly deteriorated, resulting in local hot spots along the heated surface. Based on the results of the present DNS combined with theoretical considerations, the physical mechanism of this local heat transfer deterioration is elucidated.