The aim of this work to determine the formation of lactulose during heat treatment process as a contribution to the estabilishment of limits of chemical indicators for different types of heat processed milk and analyze of lactulose for the reconstituted milk added samples. The HTST(75$\^{C}$/15s) and UHT(130$\^{C}$/2∼3s) treatment realized with a pilot plant and heat-treated samples were stroed at 4, 10, 30$\^{C}$ for 4 weeks. Changes in lactulose was evaluated at 7 days intervals. The other heat treatment was sealed in glass tube and heated at 75$\^{C}$ for 10 to 120s and heated at 130$\^{C}$ for 2 to 60s in a thermostatically controlled constant temperature bath of glycerol. The reconstituted milk was made with full fat dry milk that reconstituted with deionized water to 10% total solid, and was added to milk at 10, 20, 30% respectively. The samples processed with a HTST pilot plant showed that lactulose was contained at 1.47∼1.52mg/10()ml and 8.19 ∼8.32mg/100ml for UHT-treated samples. Changes in the lactulose content of heat-treated samples during storage at 4 and 10$\^{C}$ for 4 weeks caused a slight increase, however a noticeable increase was observed at 30$\^{C}$ for 4 week. The glass tube samples showed that high correlations between relative increase in content of lactulose and increasing processing times(75$\^{C}$ : r = 0.986, 130$\^{C}$ : r = 0.987, respectively). Added with reconstituted milk would cause a increase of the lactulose content linear with increasing addition amount(r = 0.982). This results observed for lactulose in commercial milk samples would applied to the detection of chemical changes during heat treatment and illegal use of reconstituted milk.
Journal of the Korean Society of Propulsion Engineers
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v.18
no.1
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pp.73-84
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2014
The present paper includes recent research works on the estimation of physical properties like equivalence ratio and heat release rate of flame through chemiluminescence measurement. Modern combustion devices require a precise control to increase combustion stability as well as to suppress pollutant emissions. The determination of combustion characteristics from chemiluminescence provides practical advantages over other techniques. However, the technique is dependent on equivalence ratio, combustion pressure, inlet temperature, turbulent intensity and fuel type. The intensity ratio of $OH^*$ and $CH^*$ has a strong relation with an equivalence ratio for methane/air premixed flames. The global measurement of chemiluminescence is accepted as a good indicator for a global heat release rate.
Physiological parameters were measured on six primiparous, non-pregnant Holstein cows prior to peak lactation over a 3-month summer season in southwestern Taiwan. The objectives were to characterize heat stress-induced change in functionality of mammary gland under natural climates of tropical summer and to establish physiological indices applicable to this environment in referring to this change. Environmental and physiological readings, milk and blood samples were taken at 15:00 h biweekly for totally five time points during the study. Climate readings showed that the afternoon humidex value reached the highest (53.5) around mid summer. Rectal temperature of cows taken simultaneously varied between $38.26^{\circ}C$ and $40.02^{\circ}C$ in parallel to humidex. Milk production declined drastically from 29.2 to 22.2 kg/d the first month entering summer but leveled up at end of the summer season suggesting effects exerted by heat stress rather than stages of lactation. Lactose content decreased linearly (p<0.05) with times in summer, from 4.69 to 4.38%. On the other hand, activity of N-acetylglucosaminidase (NAGase) in milk increased linearly to over two folds (p<0.05) during the same intervals. Elevations of fractional constituent of BSA in whey protein and serum cortisol level were also noticed in the course. Measurement of arteriovenous concentration (A-V) difference across the mammary gland demonstrated net uptake of glucose and net release of urea throughout the study period. The amount of urea released from mammary gland increased (p<0.05) progressively from 1.54 to 7.76 mg/dl during summer. It is concluded that gradual regression of mammary gland occurred along the humid tropical summer season. This regression is likely initiated through elevation of body temperature, which is irreversible above certain point. The increased release of urea from mammary gland during heat stress suggests its potential role as an early indicator of suboptimal mammary function.
Objectives Heat shock protein 90 (HSP90) is a highly conserved molecular chaperone important in the maturation of a broad spectrum of protein. In this study, an HSP90 gene was isolated from Asian paddle crab, Charybdis japonica, as a bio-indicator to monitor the marine ecosystem. Methods This work reports the responses of C. japonica HSP90 mRNA expression to cellular stress by endocrine disrupting chemicals (EDCs), such as bisphenol A (BPA) and 4-nonylphenol (NP) using real-time. reverse transcription polymerase chain reaction. Results The deduced amino acid sequence of HSP90 from C. japonica shared a high degree of homology with their homologues in other species. In a phylogenetic analysis, C. japonica HSP90 is evolutionally related with an ortholog of the other crustacean species. The expression of HSP90 gene was almost distributed in all the examined tissues of the C. japonica crab but expression levels varied among the different body parts of the crabs. We examined HSP90 mRNA expression pattern in C. japonica crabs exposed to EDCs for various exposure times. The expression of HSP90 transcripts was significantly increased in C. japonica crabs exposed to BPA and NP at different concentrations for 12, 24, 48 and 96 hours. The mRNA expression of HSP90 gene was significantly induced in a concentration- and time-dependent manner after BPA or NP exposures for 96 hours. Conclusions Taken together, expression analysis of Asian paddle crab HSP90 gene provided useful molecular information about crab responses in stress conditions and potential ways to monitor the EDCs stressors in marine environments.
Heat stress negatively affects milk quality altering its nutritive value and cheese making properties. This study aimed at assessing the impact of seasonal microclimatic conditions on milk quality of Friesian cows. The study was carried out in a dairy farm from June 2013 to May 2014 at Beni-Suef province, Egypt. Inside the barn daily ambient temperature and relative humidity were recorded and used to calculate the daily maximum temperature-humidity index (mxTHI), which was used as indicator of the degree of heat stress. The study was carried out in three periods according to the temperature-humidity index (THI) recorded: from June 2013 to September 2013 (mxTHI>78), from October 2013 to November 2013 (mxTHI 72-78) and from December 2013 to April 2014 (mxTHI<72). Eighty Friesian lactating dairy cows were monitored in each period. The three groups of cows were balanced for days in milk and parity. Milk quality data referred to somatic cell count, total coliform count (TCC), faecal coliform count (FCC), Escherichia coli count, percentage of E. coli, and Staphylococcus aureus, percentage of fat, protein, lactose, total solid and solid non-fat. Increasing THI was associated with a significant decrease in all milk main components. An increase of TCC, FCC, and E. coli count from mxTHI<72 to mxTHI>78 was observed. In addition, the isolation rate of both S. aureus and E. coli increased when the mxTHI increased. The results of this study show the seriousness of the negative effects of hot conditions on milk composition and mammary gland pathogens. These facts warrant the importance of adopting mitigation strategies to alleviate negative consequences of heat stress in dairy cows and for limiting related economic losses.
Journal of the Korean Society of Environmental Restoration Technology
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v.15
no.2
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pp.137-147
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2012
GRS is an effective urban ecology restoration technique that can manage a variety of environmental functions such as ecological restoration, rainwater spill control and island heat effect from a low-impact development standpoint that can be utilized in new construction and retrofits. Recently, quantitative evaluation studies, both domestic and abroad, in the areas related to these functions, including near-earth surface climate phenomenon, heavy rainwater regulation, thermal environment of buildings, have been actively underway, and there is a trend to standardize in the form of technological standards. In particular, centered on the advanced European countries, studies of standardizing the specific insulation capability of buildings with green system that comprehensively includes the green roof, from the perspective of replacing the exterior materials of existing buildings, are in progress. The limitation of related studies in the difficulties associated with deriving results that reflect material characteristics of continuously evolving systems due in part to not having sufficiently considered the main components of green system, mechanisms of vegetation, soils. This study attempts to derive, through EnergyPlus, the effects that the vegetation-related indicators such as vegetation height, FCV, etc. have on building energy load, by interpreting vegetation and soil mechanisms through plant canopy model and using an ecological standard indicator LAI that represent the condition of plant growth. Through this, the interpretations that assume green roof system as simple heat insulation will be complemented and a more practical building energy performance evaluation method that reflects numerical methods for heat fluxes phenomena that occur between ecology restoration systems comprised of plants and soil and the ambient space.
The Leadership in Energy and Environmental Design (LEED) has provided abundant resources and guidelines for a new project to become a sustainable anchor in the neighborhood. Paired with a range of checklist, LEED has strong influence on the standards for a sustainable building, and it also has played an iconic role in energy-efficient architecture. However, it is still unclear as to whether or not an LEED certified building enhances environmental benefits to its surroundings. If an LEED certification promises a baseline for an eco-friendly building, then a group of these structures should ensure significant environmental benefits to the society. This is the main question of this study, and the authors answer this hypothesis by examining the relationship of LEED certificates and their influence on outdoor temperature, especially in terms of urban heat island effect. The goal of this paper is to analyze the influence of the LEED certification on urban temperature as an indicator of sustainable architecture's regional interactions. If an LEED certificate is regarded as a strong contributor to a sustainable built environment, then a group of these certificates should result in greater benefits to society. To this extent, the authors question if there is any possible relationship between a large concentration of LEED certified sites and the temperature of their surroundings. To properly assess the research direction, Global Moran's I analysis, Local Moran's I analysis, and Hot Spot analysis are implemented to find the clustered areas of LEED certified buildings. For examining relationships between clustered area and its temperature, correlation efficients are calculated.
We have explored the impact of altering the Ras-cAMP pathway on cell survival upon oxidative exposures. A hyperactivated Ras mutant of Saccharomyces cerevisiae, intrinsically more sensitive to heat shock than the wild type, was investigated with regard to oxidative stress. In this paper we report that the response of iral, ira2-deleted mutant (IR2.53) to an oxidant, such as hydrogen peroxide (H2O2) or menadione is more sensitive than that of the wild type. IR2.53 showed a dramatic decrease in survival rate when challenged with 0.1 mM H2O2 for 30 min. The greater sensitivity of IR2.53 was also noticed with treatment of 0.01 mM menadione. Prior to oxidative stresses by these oxidants, both the wild type and the mutant were preconditioned with a mild heat shock (37 ℃, 30 min), resulting in improved survivals against oxidative stresses. Rescue of IR2.53 from menadione stress by heat pretreatment was more clearly demonstrated than that from H2O2 treatment. On the other hand, no significant difference was observed between the wild type and the IR2.53 mutant in their survival rates upon paraquat treatments. These findings imply that the mechanism by which H2O2 and menadione put forth their oxidative effects may be closely associated with the cAMP-Ras pathway whereas that of paraquat is independent of the Ras pathway. Finally, the level of glutathione (GSH) was measured enzymatically as an indicator of antioxidation and compared with the survival rate. Taken all these together, this study provides an insight into a mechanism of the Ras pathway regulated by several oxidants and suggests that the Ras pathway plays a crucial role in protection of cell damage following oxidative stress.
Journal of the Korean Society for Geothermal and Hydrothermal Energy
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v.19
no.4
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pp.39-52
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2023
This study analyzes the energy performance of a elderly care center building and the applicability of a ground source heat pump (GSHP) system through simulation approach. For this purpose, a building information modeling (BIM) program and an energy performance calculation program were used. The impact of the mechanical ventilation system on the energy requirements of the heating and cooling system and the indoor environment was also analyzed, focusing on the change in indoor carbon dioxide (CO2) concentration, which is a representative indicator of the indoor environment (air quality). The simulation results showed that the target building exceeds Level 7 in terms of simulated primary energy consumption or actual energy consumption. In addition, it was analyzed that the target building could not maintain the indoor CO2 concentration below the standard concentration by natural ventilation through window opening alone. Combining the GSHP system with the mechanical ventilation system (Case B and Case C) can further reduce the overall energy consumption by reducing the amount of outdoor air introduced by opening windows. The cost savings compared to the baseline case are estimated to be 67.3% for Case A, 63.7% for Case B, 65.5% for Case C, and 42.5% for Case D. It is necessary to analyze the impact of various renewable energy technologies and passive ones on the energy performance and indoor environment of elderly care centers.
Journal of Advanced Marine Engineering and Technology
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v.36
no.7
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pp.910-918
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2012
Carbon monoxide (CO) is a primary air pollutant as an indicator of air quality released from motor vehicle combustion. A comparative study of the distributions of CO concentration with no heat source in two tunnel models open and closed at both end sides is simulated with a commercial CFD code. The tunnel models are used to investigate the CO concentration distributions at three Reynolds numbers, which are computed by the inlet velocities of 0.3, 0.6 and 1.0 m/s. For a better tunnel design, the CFD predictive approaches are available in qualitatively studying the distributions of CO concentration. In the case of the tunnel open at both end sides in sixty seconds, the total CO concentrations are approximately twenty eight percent higher than those in the closed case.
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