• Molecules and Cells
• /
• v.37 no.6
• /
• pp.473-479
• /
• 2014

Spermatogonial stem cells (SSCs, also called germline stem cells) are self-renewing unipotent stem cells that produce differentiating germ cells in the testis. SSCs can be isolated from the testis and cultured in vitro for long-term periods in the presence of feeder cells (often mouse embryonic fibroblasts). However, the maintenance of SSC feeder culture systems is tedious because preparation of feeder cells is needed at each subculture. In this study, we developed a Matrigel-based feeder-free culture system for long-term propagation of SSCs. Although several in vitro SSC culture systems without feeder cells have been previously described, our Matrigel-based feeder-free culture system is time- and cost-effective, and preserves self-renewability of SSCs. In addition, the growth rate of SSCs cultured using our newly developed system is equivalent to that in feeder cultures. We confirmed that the feeder-free cultured SSCs expressed germ cell markers both at the mRNA and protein levels. Furthermore, the functionality of feeder-free cultured SSCs was confirmed by their transplantation into germ cell-depleted mice. These results suggest that our newly developed feeder-free culture system provides a simple approach to maintaining SSCs in vitro and studying the basic biology of SSCs, including determination of their fate.

• Journal of Korean society of Dental Hygiene
• /
• v.10 no.2
• /
• pp.297-310
• /
• 2010

Objectives : The purpose of this study was to verify the impact of the empowerment of dental personnels as one of variables affecting the performance of dental institutions and to seek ways of boosting their organizational productivity. Methods : The subjects in this study were dental personnels who worked in dental institutions. After three investigators were trained, a pilot survey was conducted from October 20 to November 20, 2009, and the questionnaire used in the pilot survey was finalized. The collected data were analyzed with a SPSS(Statistical Package for the Social Science) WIN 12.0 program. The findings of the study were as follows: Results: 1. The dental personnels investigated got 3.98 in meaningfulness, one of the empowerment factors. They found a meaning in their job when they performed it. 2. The dental personnels who were older were more excellent in self- determination and influence, and age made a significant difference to their empowerment. 3. The dental personnels who were better educated were more superior in terms of empowerment, and academic credential made a significant difference to their empowerment factors was statistically. 4. The dental personnels whose yearly salary was larger were more which were the factors of empowerment of each factor was statistically significant. 5. Dental health agency workers less than three years accounted for most of 152 people were working longer duration of empowerment as a whole was that each of the factor scores. Empowerment of each factor was statistically significant. 6. The dental hygienists made up the largest group among the dental institution employees, and the kind of occupation made a significant difference to meaningfulness, competency and influence, which were the factors of empowerment. 7. The greatest group of the dental personnels worked in dental clinics, and the dental hospital employees surpassed the dental clinic employees in competency, self-determination and influence. There was a significant difference in the influence factor according to the type of dental institutions. Conclusions : Thus, the dental personnels were more aware of the meaningfulness factor when their perception of the job-related empowerment was checked, and it indicated that most of them attached importance to meaningfulness during their job performance. But just employees who worked in dental institutions located in particular regions were examined, the findings might not be generalizable. In the future, more extensive research should be implemented, and the sorts of programs that could stir up empowerment among dental institution employees or bolster organizational empowerment should be developed.

• Korean Journal of Social Welfare Studies
• /
• v.42 no.2
• /
• pp.493-523
• /
• 2011

This study set out to investigate the effects of individual motivation on turnover intention among social workers and examine their turnover intentions in details by focusing on the mediation effects of multiple commitment. To be specific, it aimed to propose and test a prediction model for social workers' turnover intentions based on the Self-determination Theory and Theory of Planned Behavior. For those purposes, a mail survey was taken among social workers working for use facilities, residential facilities, public health centers, social welfare foundations and associations, and all kinds of centers and institutions in 15 cities and provinces across the nation. Total 1,918 questionnaires were distributed, and 1,671 ones were returned, and 979 whose respondents expressed a turnover intention were used in final analysis. The analysis results indicate that psychological motivation of social workers had direct impacts on their turnover intention. However, their role stress had no direct impacts on their turnover intention, which suggests that the impulsive routes model for turnover intention is supported only in psychological motivation and job characteristics. Secondly, their psychological and job motivation turned out to have indirect impacts on turnover intention through the multiple commitment, which suggests that the reflective routes model for turnover intention is supported in all career, job, and organizational commitment. Career commitment had the most significant impacts on turnover intention, being followed by job commitment and organizational commitment in the order, which suggests that the social welfare academy should increase their interest in career commitment. Based on the findings, the study proposed implication for the career management plans, plans for human resources

• Child Health Nursing Research
• /
• v.19 no.3
• /
• pp.149-158
• /
• 2013

Since 1990, descriptive studies about the concept of client advocacy have been published in the health journals. However, empirical studies of the concept are still lacking. There are not enough bases to apply the concept of client advocacy in nursing practice. The purpose of this paper is to encourage empirical research about client advocacy by clarification of the concept and to discusses application of the theory in child healthcare. The literature was reviewed that empirical studies on client advocacy conducted after the year 2000. The changing trends in the client advocacy concept was examined; it was changed from the philosophical concept to nursing action and changed from individual advocacy to collective advocacy, with the rise of the self advocacy concept. The research trends on client advocacy evolves from descriptive study to quasi-experimental study and instrument development study, with diversification and expansion of research methods, advocates, clients and settings. The advocacy role of the nurse in child healthcare is significant due to the child's lack of self determination ability. In ill child care, the application of individual advocacy is potent, while in healthy child care, collective advocacy, policy advocacy, and social advocacy is effective.

• The Journal of the Korea Contents Association
• /
• v.22 no.5
• /
• pp.788-797
• /
• 2022

The purpose of this study is to identify the satisfaction with medical services of the disabled elderly who have the highest need for medical services. For this purpose, the effect of health characteristics and medical service characteristics of the disabled elderly on medical service satisfaction was verified. The subjects of analysis were 3,323 persons with disabilities aged 65 or older who were taken from the national survey of people with disabilities in 2017. For statistical analysis, descriptive analysis, correlation analysis, and regression analysis were performed using the SPSS 26.0 program. The results of the study showed as follows. As a result of the regression analysis, gender (β= -.045, p<.05) and residence status (β= -.048, p<.05) among the demographic characteristics as control variables had a statistically significant effect on the level of medical service satisfaction. Among the health characteristics, IADL (β=-.044, p<.05) had a statistically significant effect on medical service satisfaction level. In the case of medical service characteristics, satisfaction with medical facilities and equipment (β = .290, p< .001), medical staff's level of understanding of disability (β = .404, p< .001), health-related service use (β = .182, p<.05) had a statistically significant effect on the level of medical service satisfaction. Based on the results, practical alternatives to ensure health equity in the community medical system were suggested in the discussion to enhance the health management and self-determination capabilities of the disabled elderly.

• Research in Community and Public Health Nursing
• /
• v.29 no.2
• /
• pp.244-256
• /
• 2018

Purpose: This study tried to understand discourses of life-sustaining treatments in general daily and healthcare newspapers. Methods: A text-network analysis was conducted using the NetMiner program. Firstly, 572 articles from 11 daily newspapers and 258 articles from 8 healthcare newspapers were collected, which were published from August 2013 to October 2016. Secondly, keywords (semantic morphemes) were extracted from the articles and rearranged by removing stop-words, refining similar words, excluding non-relevant words, and defining meaningful phrases. Finally, co-occurrence matrices of the keywords with a frequency of 30 times or higher were developed and statistical measures-indices of degree and betweenness centrality, ego-networks, and clustering-were obtained. Results: In the general daily and healthcare newspapers, the top eight core keywords were common: "patients," "death," "LST (life-sustaining treatments)," "hospice palliative care," "hospitals," "family," "opinion," and "withdrawal." There were also common subtopics shared by the general daily and healthcare newspapers: withdrawal of LST, hospice palliative care, National Bioethics Review Committee, and self-determination and proxy decision of patients and family. Additionally, the general daily newspapers included diverse social interest or events like well-dying, euthanasia, and the death of farmer Baek Nam-ki, whereas the healthcare newspapers discussed problems of the relevant laws, and insufficient infrastructure and low reimbursement for hospice-palliative care. Conclusion: The discourse that withdrawal of futile LST should be allowed according to the patient's will was consistent in the newspapers. Given that newspaper articles influence knowledge and attitudes of the public, RNs are recommended to participate actively in public communication on LST.

• Journal of Korean Public Health Nursing
• /
• v.34 no.3
• /
• pp.325-340
• /
• 2020

Purpose: This study was undertaken to identify the effects of a camp-type group counseling program based on the self-determination theory, for controlling smartphone usage amongst university students. The program consisted of smartphone overdependence, basic psychological needs, and adaptation to college life. Method: This was a quasi-experimental research with a non-equivalent control group design (pre-test and post-test design). A total of 38 freshmen (17 experimental and 21 control) participated in the study. Experimental subjects attended a two nights and three days camp, where the group counseling program included meetings for basic psychological needs (eg., autonomy, competence, and relatedness). Results: This study showed significant differences in basic psychological needs (Z=-2.91, p=.003) and adaptation to college life (Z=-3.47, p<.001). Although no significant difference was obtained in the degree of smartphone overdependence (p=.194), mean score of the experimental group pre-test and post-test changed from the level of potential risk users to general users. Conclusion: The current findings indicate that by considering the basic psychological needs and adaptation to college life, our program could be an effective intervention for controlling smartphone usage amongst college students. Therefore, we propose that a camp-type counseling program could be considered for application as group therapy for programs associated with preventing smartphone overdependence.

• Journal of Korean Public Health Nursing
• /
• v.12 no.1
• /
• pp.103-115
• /
• 1998

As the studies that smoking can be a major cause to various diseases have been made, many following researches on the outcome of stop-smoking education were in progress. Even though researches based on the knowledge about smoking and status about the teenagers were prevalent, the research based on the outcomes in long time basis were not in progress. Therefore, I tracked the people who went through 5-Day Stop-Smoking School that has taught through complexed structure of behavioral, intellectual, and psychological education. I made researches on the average of success and the hardest point during their efforts to stop just to show the necessity of going through re-education. The objectives of this study were the ones who have completed the education on the years 1990, 1991. 47 were selected from 364 people that completed the training, and who were able to be contacted on the phone line. This study was conducted from 27 Oct. to 7 Nov. 1997 through verbal interviews based on the questionnaire. The questionnaire used here was made by myself, assisted by my professor. Analysis was made through unstructured open questions. The data was analyzed using SPSS program. The major results were as follows ; 1) General characteristics of the objectives are $97.0\%$ were male, $17\%$ ages below 40s, $34\%$ in the age group of 40s, and $48.9\%$ over 50s. Religiously christian 340/0, buddhist $19.1\%$, no religion or any other reason $46.8\%$. Status married $93.6\%$, unmarried $6.4\%$. There is someone smoking in the family $36.2\%$, no one smokes $63.8\%$. Reputation salary men $55.3\%$, personal business $27.7\%$. 2) The average of success is $42.6\%(20/47)$, the failure is $57.4\%$. 3) The results from the study 'When was the hardest point in the process of stop smoking' : For the successors the first week $33.3\%$, after the first week $66.7\%$. For the failures the first week $55\%$, after first week $45\%$(Statistics not precisely done), the most effective element that helped through the hardest point was the family $40\%$, personal determination $30\%$. 4) The necessity of re-education : Successors needed $55\%$, not needed $45\%$. Failures needed $48.1\%$, not needed $51.9\%$(Statistics not precisely done). The perfect time for reeducation : Successors in 6 months $50\%$, irregular time basis $50\%$. Failures in six months $36.4\%$, after six months $27.3\%$, irregular time basis $36.4\%$(Statistics not precisely done). Synthesizing the result of the study can't generalize the long-term effect of the stop-smoking due to the number of the objectives,. but recognize the fact that 47.6 have experienced success, and also the self-determination and the support from the family are desirable. Seeing the fact that both are great motivation to stop smoking. Since the first week is necessary. The necessity of re-education is rather high, so this education should be planned to be done repeatedly in a long term along with close observation, instead of short education.

• International Journal of Computer Science & Network Security
• /
• v.22 no.6
• /
• pp.374-381
• /
• 2022

The article identifies and theoretically substantiates the trends of national resilience in the context of establishing the security of the country and its civilizational subjectivity. The strategy of development of the pedagogical university in the conditions of European integration into the European educational and scientific space based on certain characterological features of the personality of the volunteer in the context of allocation of personal resilience is developed. The analysis of both external and internal challenges and threats to the civilization of the country needs to be understood in the context of economic, socio-political, legal, military-political, spiritual-cultural, educational-scientific and network-information resilience. The concepts of "national resilience" and "national security" are quite close - at first glance, even identical. However, a deeper understanding clarifies the differences: national security is a state of protection of the country identity and its very existence, the realization of its national interests. In turn, resilience is a fairly effective strategy and a fundamental guarantee of national security. At the same time, it is extremely important to understand that both national security as a state and national resilience as a strategy are only means of achieving and developing a strong and humanistic civilizational subjectivity of the country. After all, such subjectivity opens for citizens the opportunity for development, dignified self-realization and a proper life. The restructuring of the volunteer's motivational sphere is due to the dominance of such leading motives, which are focused mainly on maintaining and restoring health, which leads to distorted meaningful life goals: isolation, alienation, passivity, inertia, reduced activity, limited communication, etc. The characteristics of relatively stable human behavior include several primary and secondary properties. The primary (relevant) properties include patience, trust, hope, faith, confidence, determination, perseverance, and love; the secondary - punctuality, neatness, obedience, honesty, loyalty, justice, diligence, thrift, accuracy, conscientiousness, obligation, etc. The restructuring of the volunteer's motivational sphere is due to the dominance of such leading motives, which are focused mainly on maintaining and restoring health, which leads to distorted meaningful life goals: isolation, alienation, passivity, inertia, reduced activity, limited communication, etc. The characteristics of relatively stable human behavior include several primary and secondary properties. The primary (relevant) properties include patience, trust, hope, faith, confidence, determination, perseverance, and love; the secondary - punctuality, neatness, obedience, honesty, loyalty, justice, diligence, thrift, accuracy, conscientiousness, obligation, etc. The use of information and communication technologies in volunteering will contribute to the formation of resilience traits in the structure of personality formation. Directly to the personal traits of resilience should be included methodological competencies, which include methodological knowledge, skills and abilities (ability to define ultimate and intermediate goals, plan, conduct and analyze knowledge, establish and implement interdisciplinary links with disciplines of medical-psychological-pedagogical cycles, etc.). All these competencies form the professional resilience of the volunteer.

• Journal of the Korean Dietetic Association
• /
• v.10 no.3
• /
• pp.333-344
• /
• 2004

To investigate current status of the elderly meal service program, community centers nationwide were surveyed for congregate meal services. The survey was conducted during the month of December 2002 by mail using self-developed questionnaire on administration, staffs, funding, and meal service management. Only 95 out of 356 community centers (27%) answered and returned the questionnaires, and congregate meal menus were also collected from 20 centers for 492 days. Results were analyzed using SAS package program. In more than half of the elderly congregate meal service programs, following criteria were used in multiple form to allow participation; 65 years old or older, low income, or living alone. All centers served lunch, most of them free of charge, and 88.4% of them provided service 5 days a week. About 79% of the total cost occurred on the congregate meal service was supported by the government. Most of the menus served at congregate meal service were Korean style dish with rice. On the average, each meal provided 3 side dishes including kimchi, in addition to soup or stew. Only 19% of the centers have employed dietitian working for meal service programs; meal menus were prepared by social welfare workers or other non-dietetic personnels in more than 80% of the centers. Food purchasing and food hygiene control was also practiced mainly by social welfare workers or cooks. This study suggests that dietetic professionals are needed to better manage meal service programs for the elderly in both aspects, food hygiene and nutrition. Development of more nutritious menus and determination of appropriate serving sizes for the elderly meal service programs are necessary to meet the RDAs and dietary action guide for the elderly.