• Journal of Microbiology and Biotechnology
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• v.27 no.5
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• pp.943-946
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• 2017

Although the genus Leuconostoc, generally found in various fermented foods, has often been suggested to be a novel probiotic for food fermentation and health promotion, the strains in this genus showed low acid tolerance and low osmotic stress resistance activities, which are required for survival during food fermentation events. Recently, a novel species of Weissella, W. jogaejeotgali $FOL01^T$ (= KCCM 43128 = JCM 30580), was isolated from Korean fermented clams. To determine the genomic features of this new species, its genome was completely sequenced and analyzed. The genome consists of a circular chromosome of 2,114,163 bp of DNA with a G+C content of 38.8%, and the plasmid pFOL01 consists of 35,382 bp of DNA with a G+C content of 39.1%. The genome analysis showed its potential for use in food fermentation and osmotic stress resistance abilities for processing in food industries. In addition, this strain was predicted to have acid tolerance and adhesion to the mucosal layer for survival and colonization in the gut. Subsequent experiments substantiated these abilities, suggesting that W. jogaejeotgali may have probiotic potential and a high survival rate during food fermentation. Therefore, it may be suitable as a novel probiotic strain for various applications in food industries.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.2
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• pp.749-755
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• 2014

Background: Purple rice has become a natural product of interest which is widely used for health promotion. This study investigated the preventive effect of purple rice extract (PRE) mixed diet on DMH initiation of colon carcinogenesis. Materials and Methods: Rats were fed with PRE mixed diet one week before injection of DMH (40 mg/kg of body weight once a week for 2 weeks). They were killed 12 hrs after a second DMH injection to measure the level of $O^6$-methylguanine and xenobiotic metabolizing enzyme activities. Results: In rats that received PRE, guanine methylation was reduced in the colonic mucosa, but not in the liver, whereas PRE did not affect xenobiotic conjugation, with reference to glutathione-S-transferase or UDP-glucuronyl transferase. After 5 weeks, rats that received PRE with DMH injection had fewer ACF in the colon than those treated with DMH alone. Interestingly, a PRE mixed diet inhibited the activity of bacterial ${\beta}$-glucuronidase in rat feces, a critical enzyme for free methylazoxymethanol (MAM) release in the rat colon. These results indicated that purple rice extract inhibited ${\beta}$-glucuronidase activity in the colonic lumen, causing a reduction of MAM-induced colonic mucosa DNA methylation, leaded to decelerated formation of aberrant crypt foci in the rat colon. Conclusions: The supplemented purple rice extract might thus prevent colon carcinogenesis by the alteration of the colonic environment, and thus could be further developed for neutraceutical products for colon cancer prevention.

• Journal of Korean Physical Therapy Science
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• v.5 no.4
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• pp.795-807
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• 1998

The purpose of this study was to investigate the effects of exercise therapy to oldwomen's muscle strength, flexibility and IADL. To identify the effect of exercise therapy on oldwomen's muscle strength, flexibility and IADL, this study attempted to determine grip strength, back strength, leg strength, flexibility of upper and low(flexion, extension, abduction), flexibility of trunk(flexion, extension) and IADL, using dynamometer, goniometer and Geriatric center IADL, for 34 female. Thirty-four subjects participating in this study consisted of A group(from sixty-five to sixty-nine, eleven person), B group(from seventy to seventy-four, twelve person), C group(over seventy-five years old, eleven person). Data from the findings of this experiment were computed for and standard deviation by using repeated measurement of MINI TAB. As a result, the following conclusion was drawn : 1. Subject group showed significantly higher scores on effect of exercise between three age group after regular exercise. 2. Subject group showed significantly higher scores on muscle strength(grip strength, back strength, leg strength) and there is a difference between three age group after regular exercise of muscle strength. 3. Subject group showed significantly higher scores on flexibility (upper, lower, trunk) and there is a difference between three age group after regular exercise of flexibility. 4. Subject group showed significantly higher scores on IADL(Instrumental activities of daily living) and there is a difference between three age group after regular exercise. As a result of this study, the effect of exercise training program had improved muscle strength, ROM (range of motion) of joint, IADL. Thus exercise training program could be beneficially applied for the prevention of disability and promotion of health and wellbeing in the aged easily and safely.

• The Journal of the Korea Contents Association
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• v.18 no.1
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• pp.250-257
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• 2018

Today, the integration of the rapid development of ICT and the smart devices moves our lives quickly into an online community environment through not only quick and easy information search but also various social media. Accordingly, individual activities in the smart media environment are pouring out vast quantities of data in many fields, accumulating a tremendous amount of data. The everyday data of individuals is reproducing different values from the previous ones, while suggesting new customized services that utilize them in various fields. Recently, big data utilization has attracted a great attention in the field of healthcare. Especially, development of healthcare service linked with mobile is expected to bring a new paradigm in this field. In this study, creation of a 3D avatar character model as a means to transfer information to individuals more efficiently is proposed in the development of mobile customized service for health promotion and growth prediction of children and adolescents, at the same time, an effective visual expression method to have a sense of immersion and unity is searched.

• Journal of the Korean housing association
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• v.18 no.4
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• pp.37-48
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• 2007

Purpose of this study is to investigate influence on preference of cohousing (eg. intention to spread and intention to move in cohousing) according to sense of community, especially in housing life by married people residing in Seoul and its outskirts. Research method used for this study was a social survey. Respondents were 214 people by accidential sampling. The collected data were analyzed by SPSS 12.0 program using frequency, mean, average, T-test, one-way Anova, Duncan's Multiple Range test and Chi-square test. Findings of this study were as follows. Most respondents took part in interactive activities with colleagues from the same occupation. They Focused on the issues about health, leisure and hobby. The respondents mainly wanted to have interaction for changing recycling materials and education programs for children and juveniles. Degree of ideal relationships with neighbors, they think, were greeting and chatting as well as communication about issues on child-care, education for children, offense-prevention and safety. Although respondents were interested in community life and in interaction with their neighbors, they did not build up communal society due to lack of a sense of belonging in their living areas. While respondents showed positive response about preference of cohousing, there were difference between intention to spread of cohousing and intention to move in cohousing. As a result of this study, the stronger sense of community in housing life meant the more interests in thoughts to spread and to move in cohousing. Therefore, in order to increase sense of community in cohousing, the plan of various communication programs that enables frequent interaction among neighbors is needed. Also, in order to spread cohousing in Korea, promotion of cohousing community and cognitions about it are the most needed.

• Stress
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• v.26 no.4
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• pp.259-267
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• 2018

Background: The purpose of this study was to identify the risk factors influencing cognitive function and life satisfaction in elderly living alone. Methods: It was cross-sectional descriptive study by using secondary data from the 2014 national survey of Korean elderly. The study sample consisted of 2,249 subjects who live alone, aged 65 years old and over. Data were analyzed frequency analysis, ${\chi}^2$-test, t-test, ANOVA and Logistic regression analysis. Results: The risk factors that significantly influenced cognitive function were old age, bereavement, lack of education, no regular exercise and low socio-cultural activity satisfaction. The risk factors that significantly influenced life satisfaction were no bereavement, lack of education, no work, negative evaluation of health status, diagnosis of depression and no regular exercise. Also experience of emotional and economical abuse, neglect of financial support and low cognitive function were identified risk factors of life satisfaction. Conclusions: The elderly living alone with risk factors of cognitive decline should be interested. To prevent or delay cognitive impairment, regular exercise and socio-cultural activities were recommended. It was suggested that the promotion of cognitive function, appropriate work and exercise, and social efforts to prevent abuse and neglect can contribute to improving life satisfaction of elderly living alone.

• International Journal of Advanced Culture Technology
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• v.10 no.4
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• pp.137-147
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• 2022

In this study, for students who gave up their studies midway through college, we investigate the reasons for dropping out of college, analyze the factors that caused students to drop out, and suggest improvement measures to effectively increase the recruitment rate of enrolled students. This study explores measures to increase student enrollment in community colleges. For this purpose, it conducted a survey of students who dropped out of G College between 2018 and 2020 from June 28 to July 9, 2021. Its analysis is based on the results of 53 students who participated in the survey. First, our results suggest that programs to develop interpersonal relationships between students, faculty, and staff are necessary. Such programs will cultivate a culture of solidarity and collective identity among students, which in turn will reinforce positive experiences in college. Moreover, by developing systems to form relationships between faculty and students, colleges can have a feedback mechanism, such as an open-study program, through which they learn what the students want and need. Through this effort, colleges can help their students adjust to campuses and enhance student satisfaction in college. Second, it is necessary to develop various extracurricular programs not only for freshmen but existing students and to run hobby and leisure activity programs. To have continuous, standing extracurricular programs instead of one-time events, colleges should set up specific goals, delivery methods and strategies. Third, colleges should renovate old and outdated buildings and facilities on campus to enhance the quality of campus life. Moreover, more comprehensive improvement of facilities and a campus environment by having various convenient and leisure facilities that meet the needs and demands of students. Fourth, it is suggested to develop programs or systems that help students to more fully engage in campus lives and activities, which in turn increases confidence and self-efficacy among students. Through such programs, students can better adjust to their majors and, therefore, will be less likely to drop out of college.

• The Journal of the Convergence on Culture Technology
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• v.9 no.6
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• pp.9-15
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• 2023

This study investigated the effect of self-control on life satisfaction of youth participating in drone programs. Youth participating in drone program activities in the metropolitan area (Seoul, Gyeonggi) were conducted as questionnaires. The survey data were coded and statistically analyzed. The statistical significance level was ⍺= .05. The results of the study are as follows. First, simple task preferences and physical activity preferences affect mental health. Second, the pursuit of adventure affects happiness. Third, pursuit of adventure and self-centered factors affect autonomy and self-esteem. In conclusion, it can be judged that adolescents are partially satisfied with their lives through self-control.

• Industry Promotion Research
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• v.5 no.2
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• pp.41-50
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• 2020

WIn Korea, the rapid rise of elderly population and dementia prevalence resulting from the unprecedently rapid aging in the world is being emerged as a serious social issue. Caring the elderly with dementia is a different way from a general life and is a world which new cultural existential relationships are interlaced from the relationship viewpoint. From this aspect, the psychological matter of family caring a demented elderly needs to be studied for existential understanding in depth and fundamentally. This study focused on in-depth understanding and description of the stress phenomena experienced by family caregivers with a demented elderly based on Giorgi's method among phenomenological research methods and using existential group counseling techniques. Total 8 sessions of existential group counseling were conducted. This study drew out the outcomes(emotional stress, psychosocial stress, time-dependent stress) of participants' self-awareness experiences by applying 4 steps of Giorgi's phenomenological research methodology based on the Existential Philosophy to participant's intensive statements about stress of family caring a demented elderly drawn out of the existential group counselling sessions. It is hoped that understanding the pain of the supporters who cannot express their own pains through the research results and their self-help group counseling activities will become active, contributing to the health of our society which is about to enter post-aged society.

• Journal of Veterinary Clinics
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• v.22 no.4
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• pp.386-391
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• 2005

This study was done to produce a mutated protein inactivated cytotoxicity of Shigatoxin 2e (Stx2e) of E.coli O139 isolates by deletional mutagenesis of Stx2e A subunit gene encoding active-site cleft of enzymatic domain in ST2e holotoxin. Cytotoxicity of the toxoid expressed from the mutant Stx2e gene was compared with wild type Stx2e for development of vaccine candidate. A recombinant plasmid pED18 containing Stx2e gene ot E.coli O139 isolates was used to generate mutation plasmid. Deletion mutagenesis was conducted for Stx2e A subunit gene encoding enzymatically active domain by polymerase chain reaction (PCR) using ot designed primer to induce deletional mutation. DNA sequence analysis was confirmed that the pentamer (Typ 202- Ser 206) that lies within the proposed active-site cleft in the second region was completely deleted. A DNA fragment of 1.1 kb that encode the new mutant Stx2eA gene was inserted into plasmid pRSET vector digested with EcoRV-Hind III and named pEDSET The PEDSET was transformed in E. coli for expression of mutant protein and the protein was confirmed by SDS-PACE and Western-blotting. The protein expressed by the mutant was tested to confirm the reduction of cytotoxic activities on Vero cell using microcytotoxicity assay compared with wild type Stx2e, the cytotoxicity of deletional mutant protein was at least reduced by 3,000-fold on Vero cell.