• 생약학회지
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• 제50권1호
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• pp.37-45
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• 2019

Portulaca oleracea L. (PL) has been used in traditional medicine herb for treatment of various diseases, such as diarrhea, dysentery, and skin inflammation. Previous studies have shown that the PL regulates the inflammation by inhibition of pro-inflammatory cytokines. Although PL might have improvement effects of intestinal function and bioactive effects, there are not enough studies to demonstrate. This study investigated the effects of KDC16-2 on the improvement of intestinal function and anti-inflammatory effects in vivo and in vitro. The improvement effect of intestinal function was measured fecal amount, water content and intestinal transit rate in KDC16-2 treated ICR mice. As results, compared with the control group, the KDC16-2 group showed a significant increase in wet fecal weight, dry fecal weight and fecal water content. The intestinal transit rate of KDC16-2 group was significantly increased. Based on the results, KDC16-2 is considered to have effects on improving intestinal function. The effect of anti-inflammatory demonstrated by using dextran sulfate sodium (DSS)-induced colitis mice. The mice were administered 3% DSS along with KDC16-2 (100, 300 mg/kg) for 14 days. DSS-induced colitis mice were significantly ameliorated in KDC16-2 treated group, including body weight loss, colon length shortening, tight junction protein of colon and histological colon injury. The levels of inflammatory mediators (IgG2a, IgA, C-reactive protein and Myeloperoxidase) and pro-inflammatory cytokines (tumor necrosis factor (TNF)-${\alpha}$, Interleukin (IL)-6) which are involved in inflammatory responses were increased in the DSS-treated group as compared to those in the control group, and the levels were significantly decreased in the KDC16-2 groups. In addition, we investigated the impact of KDC16-2 on lipopolysaccharide (LPS)-induced inflammatory responses in J774A.1 cells. KDC16-2 inhibited production of prostaglandin E2 (PGE2) and reactive oxygen species (ROS). These results suggested that the KDC16-2 could effectively alleviate the dysfunction of intestinal and inflammatory mediators. Thus, these KDC16-2 can be potentially used as health functional food of intestinal.

• 한방안이비인후피부과학회지
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• 제32권2호
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• pp.68-93
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• 2019

Objective : This study summarized the characteristics of clinical trials for atopic dermatitis medicines approved by the Ministry of Food and Drug Safety(MFDS). This study may be a reference for the design of clinical trials of atopic dermatitis herbal medicine treatment which may be carried out later. Method : The characteristics of the clinical trial were analyzed for clinical trials registered with ClinicalTrials.gov, CRIS, and the Korea Health Industry Development Institute among the clinical trial approval statuses posted on the website of the MFDS. Result : 1. Clinical trial drugs were developed in various formulations such as oral medicines, injections, dermatologic agents, and similar proportions. Relatively little clinical trials were found for herbal medicine. 2. In the control evaluation test, most of the treatments for the control group were performed with placebo using Vehicle. 3. In most clinical trials, one intervention group was in the form of a parallel assignment with only one treatment. 4. The age of the subjects was 11 out of 28 studies including minors, and clinical trials targeting minors were also found to be significant. 5. In the case of atopic dermatitis, the cases of subacute chronic or atopic dermatitis more than 6 months or more than 1 year were often used. 6. Most clinical trials were divided into mild to moderate atopic dermatitis or moderate to severe atopic dermatitis. The SCORAD index, EASI, IGA, BSA, and NRS were used as the evaluation criteria. 7. Regulations for the drugs used prior to the trial period for the treatment of atopic dermatitis vary somewhat from one clinical trial to another. 8. IGA was used most often as a primary efficacy tool, and SCORAD index, EASI, and NRS were also used.

• 한국정보통신학회:학술대회논문집
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• 한국정보통신학회 2019년도 춘계학술대회
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• pp.32-35
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• 2019

축산업의 큰 문제점 중에 하나는 여름철이 되면 폭염이나 전염병으로 인하여 가축들이 폐사하는 것이다. 이러한 상황에서 가축들에게 필요한 것은 적절한 실내 온도와 주기적인 살균 시스템이다. 따라서 본 연구에서는 축사 내부의 온도에 따라 선풍기의 전원을 자동으로 제어하는 시스템과 주기적으로 먹이와 살균처리를 제공하는 기능을 개발하여 효율적으로 축사를 관리할 수 있도록 연구를 진행하였다. 또한 선풍기의 전원을 자동으로 제어하는 기능과 모바일 애플리케이션에 축사 내부의 온도를 표시하는 기능, 먹이와 살균처리를 제공하는 기능을 제안하였다. 첫 번째로 선풍기의 전원을 제어하는 기능은 축사 내부의 온도가 일정 이상으로 올라가면, 선풍기가 자동으로 켜지게 한다. 반대로 축사 내부의 온도가 일정 이하로 내려가면, 선풍기는 꺼지도록 한다. 두 번째로 모바일 애플리케이션의 기능은 축사 내부의 온도를 확인하는 기능이다. 세 번째의 먹이 제공 기능은 서보모터를 이용하여 주기적으로 먹이를 제공하며 제공된 먹이는 컨베이어 벨트를 통하여 가축들에게 전달된다. 마지막으로 살균처리 기능은 DC모터 펌프를 이용하여 주기적으로 가축들을 살균하여 주는 기능이다. 본 연구에서 제안한 지능형 축사의 기능을 통하여 가축들의 건강에 기여할 수 있을 것이다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권5호
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• pp.711-720
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• 2019

Objective: This study was conducted to assess the effect of potassium humate and enzymes (Xylanase+Amylase+Protease) inclusion in diets on carcass characteristics, meat quality and fatty acid profiles of broilers fed canola-based diets. Methods: Two hundred and twenty broilers randomly allotted to 5 dietary treatments: the control (commercial broiler diet); CM (17.5% canola meal inclusion); CMEnz (17.5% CM inclusion+0.3 g/kg Axtra XAP); CMPh (17.5% CM inclusion+1.5% Potassium Humate, PH); and CMEnzPh (17.5% CM inclusion+1.5% PH+0.3 g/kg Axtra XAP) were slaughtered at day 42 for assessment of carcass and meat quality parameters. Results: Diet had no effect on carcass traits apart from breast muscle weight and breast muscle index. The highest breast muscle weight was observed in broilers fed CMEnz ($487.6{\pm}17.5g$) followed by those fed the control diet ($474.37{\pm}17.5g$). Diet also had no significant dietary effect on pH, temperature, drip loss and shear force values of the breast muscle. However, diet significantly affected meat colour and water-holding capacity. Broilers in the control and CMPh groups ($52.94{\pm}0.67$ and $52.91{\pm}0.67$) had the highest (p<0.05) values for lightness ($L^*$), whilst those fed CMEnzPh had the lowest value ($47.94{\pm}0.67$). In contrast, CM group had the lowest (p<0.05) value for redness ($a^*$) with CMEnzPh group having the highest values. The proportion of polyunsaturated fatty acids (PUFAs), n-6 and n-3 fatty acids and the PUFA/saturated fatty acid ratio were increased in CM-based diets containing enzymes and humic acid. Conclusion: It can, therefore, be concluded that CM can be included in broiler diets in the presence of enzymes and humic acid with positive effects on meat quality and important fatty acids that are beneficial to the health of consumers.

• Journal of Nutrition and Health
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• 제54권5호
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• pp.515-533
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• 2021

본 연구는 유아를 대상으로 한 영양교육의 중재효과를 평가하고, 과일·채소의 선호도 및 영양지식 향상에 효과적인 영양교육 유형을 분석하기 위해 체계적 문헌고찰과 메타분석을 수행하였다. 메타분석의 효과크기 결과에 따르면 유아 대상 영양교육은 과일·채소 선호도 및 영양지식을 유의적으로 향상시켰으며, 일반영양교육 보다는 과일·채소 중심 영양교육이 유아의 과일·채소 선호도 및 영양지식 향상에 더 효과적이었다. 또한 유아의 과일·채소 선호도 및 영양지식 향상을 위해서는 원예활동, 요리활동 및 오감활동 등의 직접 노출 활동을 포함하는 영양교육이 더욱 효과적이라는 것을 확인할 수 있었다. 본 연구는 유아대상 영양교육의 효과를 입증했을 뿐만 아니라, 유아의 과일·채소 선호도 및 영양지식 향상에 가장 효과적인 영양교육의 형태를 도출했다는 점에서 의의가 있다.

• Journal of Nutrition and Health
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• 제54권3호
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• pp.262-276
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• 2021

본 연구는 전라북도 일부 지역 중·고등학생을 대상으로 아침식사대용 쌀 기반 HMR 인식과 그 선호도를 조사하였으며, 요약하면 다음과 같다. 조사대상자의 아침식사 결식 여부 행태는 아침식사군 (주 4회 이상 섭취군)이 272명 (54.6%), 아침결식군 (주 3회 이하 섭취군)이 226명(45.4%)으로 조사되었다. 조사대상자의 HMR 섭취 만족도는 고등학생 (48.2%)이 중학생(39.1%)과 비교 시 유의적으로 HMR 만족도가 높았으며, 여학생이 남학생 보다 HMR 만족도가 높았다. 조사대상자의 아침식사 대용식의 필요성조사 결과는 남학생이 여학생보다 유의적으로 높은 비율로 응답하였다 (p < 0.01). 아침식사 대용식으로써 쌀 기반의 한식 식사 패턴의 선호도는 아침식사자가 58.9%로 아침결식자보다 높고, 고등학생이 중학생보다 유의적으로 높은 것으로 나타났다 (p < 0.01). 또한 조사대상자의 아침식사대용으로 쌀 기반 HMR 선호 식품은 '삼각김밥', '주먹밥', '김밥' 순으로 나타났다. 따라서 청소년들의 아침결식의 큰 이유인 '시간이 없어서'와 '입맛이 없어서'를 고려하여 손쉽게 섭취할 수 있는 아침식사대용 간편식 메뉴 개발 연구는 지속적으로 이루어져야 하고, 해당 메뉴의 조리교육 및 레시피 보급이 필요할 것으로 사료된다.

• 한국양봉학회지
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• 제32권3호
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• pp.253-259
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• 2017

충치발생의 주요 원인균으로 알려진 S. mutans를 이용하여 건강기능식품으로 사용되는 프로폴리스를 9개 지역으로부터 수집한 후 생리활성 물질 탐색과 주요성분 함량에 따라 활성차이를 조사하였다. S. mutans에 대한 항균활성은 제주도, 경상북도, 경상남도, 충청북도, 충청남도, 전라북도, 전라남도, 경기도, 강원도 프로폴리스 및 pinocembrin에서 모두 관찰되었다. 제주도를 제외한 모든 프로폴리스에서 총 플라보노이드 함량이 10mg/g 이상으로 건강기능식품 기준 및 규격에 적합하였고, 19mg/g 이상일 경우 우수한 항균활성을 유사하게 나타내었다. 국산 프로폴리스의 주요성분으로 알려진 pinocembrin의 UPLC 정량분석에서는 제주도산 프로폴리스를 제외한 모든 시료에서 주성분으로 검출되었고 12mg/g 이상일 경우 항균활성에 큰 영향을 주는 것으로 확인되었다.

• Journal of Microbiology and Biotechnology
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• 제31권2호
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• pp.207-216
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• 2021

Supplement of high-protein food plays an important role in improving the symptoms of malnutrition and the immune capacity of the body, but the association of high-protein diet and gut microbiota remained unaddressed. Here, we systematically analyzed the internal organs and gut microbiota in C57(WT) or PD-1H-depleted (KO) mice (T cells were activated) fed with pupae or feed for six weeks. We observed that the body weight gain in the mice fed with pupae increased less significantly than that of the feed group, while the villi and small intestine lengths in the pupa group were reduced compared with that of mice given feed. However, the average body weight of the KO mice increased compared with that of the WT mice fed with pupae or feed. Pupae increased the concentration of blood glucose in WT, but not in KO mice. Moreover, in the feed group, there was no difference in the weight of the internal organs between the WT and KO mice, but in the pupae-fed group, liver weight was decreased and spleen weight was increased compared with that of KO mice. The amounts/plural/amounts of Melainabacteria, Chloroflexi, and Armatimonadetes were specifically upregulated by pupae, and this upregulation was weakened or eliminated by PD-1H depletion. Some bacteria with high abundance in the feed-fed KO mice, such as Deferribacteres, Melainabacteria, Acidobacteria, Bacteroidetes, Spirochaetes and Verrucomicrobia, were decreased in pupae-fed KO mice, and Proteobacteria and Deinococcus were specifically enriched in pupae-fed KO mice. Bacteroidetes, Firmicutes and Akkermansia were associated with weight loss in the pupae-fed group while Lachnospiraceae and Anaerobiospirillum were related glucose metabolism and energy consumption. Based on high-throughput sequencing, we discovered that some gut bacteria specifically regulated the metabolism of a high-protein diet, and PD-1H deficiency improved life quality and sustained blood glucose. Moreover, PD-1H responses to high-protein diet through modulating the type and quantity of gut bacteria. These findings provide evidence about the association among gut microbiota, T cell activation (for PD-1H depletion) and high-protein diet metabolism, have important theoretical significance for nutrition and health research.

• Journal of Nutrition and Health
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• 제54권6호
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• pp.584-593
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• 2021

2020 한국인 영양소 섭취기준에서 탄수화물은 만성질환 예방을 위한 섭취기준으로 총 에너지 섭취량에 대한 탄수화물로부터의 에너지 섭취비율인 에너지적정비율을 1세 이후 모든 연령에서 55-65%로설정하였다. 그리고 2020년에 처음으로 탄수화물의 평균필요량과 권장섭취량을 설정하였다. 인체의 탄수화물 필요량은 뇌에서 하루에 소비되는 포도당량과 케톤체 생성이 나타나지 않는 양을 기준으로 1세 이후 모든 연령에서 1일 100 g을 설정하고, 권장섭취량은 15% 변이계수를 사용하여 두 배의 변이계수를 더한 130 g/일로 설정하였다. 탄수화물의 평균필요량은 최소필요량의 개념이지 에너지원으로서 적절한 섭취량에 대한 개념은 아니므로 탄수화물의 영양소섭취상태를 평가할 경우에는 평균필요량이나 권장섭취량에 비교하는 것은 적절하지 않고, 에너지적정비율을 활용하여 탄수화물로부터의 에너지 섭취비율의 적절성을 평가하는 것이 적절하다. 탄수화물은 에너지 적정비율범위에서 섭취할 때 만성질환의 위험을 낮출 수 있으며, 이를 탄수화물의 양뿐만 아니라 급원도 고려하는 식생활에 활용해야 할 것이다.

• 대한의용생체공학회:의공학회지
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• 제42권6호
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• pp.259-267
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• 2021

Recently, the market for personal health care and medical devices based on Bluetooth Low Energy(BLE) has grown rapidly. BLE is being used in various medical data communication devices based on low power consumption and universal compatibility. However, since data errors occurring in the transmission of medical data can lead to medical accidents, it is necessary to analyze the causes of errors and study methods to reduce data error. In this paper, the minimum communication speed to be used in medical devices was set to at least 800 byte/sec based on the wireless electrocardiography regulations of the Ministry of Food and Drug Safety. And the data loss rate was tested when data was transmitted at a speed higher than 800 byte/sec. The factors that cause communication data error were classified, and the relationship between each factor and the data error rate was analyzed through experiments. When there were two or more activated peripherals connected to the central, data error occurred due to channel hopping and bottleneck, and the data error rate increased in proportion to the communication distance and the number of activated peripherals. Through this experiment, when the BLE is used in a medical device that intermittently transmits biosignal data, the risk of a medical accident is predicted to be low if the number of peripherals is 3 or less. But, it was determined that BLE would not be suitable for the development of a biosignal measuring device that must be continuously transmitted in real time, such as an electrocardiogram.