• Journal of Applied Biological Chemistry
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• v.57 no.3
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• pp.189-196
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• 2014

In Korea, over 4,700 animal carcass disposal sites were installed until 2011 due to the outbreak of foot and mouth disease. Due to the putrefaction of buried animals, the leachate containing veterinary antibiotics may release into surrounding environments. Antibiotic residues in the environment cause the formation of antibiotic resistance bacteria threatening human and ecosystem health. This study reports the concentrations of five antibiotics, including tetracycline (TC), chlortetracycline (CTC), oxytetracycline (OTC), sulfamethazine (SMZ), and sulfamethoxazole (SMX), in soils from animal carcass disposal site and adjacent agricultural field. Concentrations of antibiotics at animal carcass disposal sites (TC: $144.26-350.73{\mu}g/kg$, SMZ: $17.72-44.94{\mu}g/kg$) were higher than those at agricultural field (TC: $134.16-320.73{\mu}g/kg$, SMZ: $6.48-8.85{\mu}g/kg$) whereas the concentrations of CTC, OTC, and SMX were below detection limit in both sites. Results showed that the antibiotics in animal carcass site might leach to the soil and possibly contaminating the groundwater. Future studies will focus on the transfer of antibiotics residues into food crops.

• Korean Journal of Veterinary Research
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• v.49 no.3
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• pp.257-263
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• 2009

Bovine mastitis is an important disease causing serious economic loss in dairy production and food poison in public health. Escherichia coli and Staphylococcus aureus are the major causative agents of bovine mastitis. These bacteria were found in milk and environmental condition such as feces, water, soil and so on. Bovine mastitis causative micro-organisms can survive in 1-2 weeks in feces and bed complexes. Low level of percentage of water content (PWC) of feces and bed complexes can reduce the spreading of bovine mastitis incidence from environmental contamination. In this study, we developed the fecal dehydrating system and determined the elimination rates of bovine mastitis causative agent from feces and bed complexes. To develop the fecal dehydrating system, the screw pressurized dehydrating system was used and the maximum rate of dehydrating was reached to 52% PWC using 90% PWC (wet base) of fecal and bed complexes. The elimination rates of the dehydrating system for E. coli and S. aureus were reached at 41.19 $\pm$ 7.84% to 62.55 $\pm$ 8.71% in various percentages of PWC of feces and bed complexes (80, 85 and 90%). These results suggested that the application of fecal dehydrating system would reduce the exposure of dairy cattle to bovine mastitis causing agents contaminated feces and bed complexes, and can be used for environmental bovine mastitis control avoiding misuse or abuse of chemical disinfectants and antibiotics in dairy farm.

• Korean Journal of Human Ecology
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• v.16 no.4
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• pp.849-854
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• 2007

Body fat proportion is a major issue in health. The prevalence of obesity in childhood has been increasing on thesedays. A series of indirect estimates of body fat have been developed. There are hydrodensitometry, BIA and anthropometry equation for body fat. Hydrodensitometry has been regarded as a common criterion method. BIA made it possible to analyze body fat mass more related to hydrodensitometry than anthropometry. The purpose of this unvestigation were to compare body fat and other body composition between boys & girls and to know which anthropometry equation for body fat was most closely associated with BIA in boys & girls respectively. The subjects were 148 4th grade elementary school students(male:70, female:78). They underwent BIA(InBody 3.0, Biospace Korea)to determine body fat and other body composition. Anthropometric measurements were taken of height, weight, skinfolds and circumferences. The results obtained are summarized as follow: 1) Weight, BMI and Rohrer index were $39.9{\pm}7.2kg$, $20.5{\pm}8.0$, $138.2{\pm}17.0$ for boys and $35.9{\pm}6.7kg$, $18.2{\pm}2.6$, $96.6{\pm}13.6$ for girls. 2) Total body fat and percent body fat determined by BIA were $10.3{\pm}4.3kg$, $25.4{\pm}6.6%$ for the boys group and $9.3{\pm}3.8kg$, $25.1{\pm}6.0%$ for girls. 3) Mean triceps skinfold thickness was $20.5{\pm}6.1mm$ in boys, $17.0{\pm}5.1mm$ in girls and mean WHR was $0.88{\pm}0.4$ in bodys, $0.82{\pm}0.4$ in female and the values were significantly different by sex. 4) Correlational nanlyses showed that estimation of body fat by BIA measurement seemed to be closely associated with Rohere index(boys r=0.854, girls r=0.909). Kim's equation was highly correlated with the body fat in girls(r=0.910) but less correlated in boys(r=0.710). We need to develop specific anthropometric equations based on age and sex to determine body fat for children.

• Korean Journal of Environment and Ecology
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• v.26 no.3
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• pp.463-471
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• 2012

Objective of this study is to provide basic information for developing more differentiated and targeted forest healing policy and Korean medical healing programs grounded on consumer preference for forest based Korean medical healing tourism products. The internet survey(CAWI) by percentage quota sampling with 400 Seoulite ages over 30 by the age, area, and gender was conducted, and 317 samples were used for a final analysis. 61.5% of the Seoulite associated 'forest bath/walking in the woods/tree' with an image of a forest based Korean medical healing tourism product, and preference for the product and the intention to use were positive at the percentages of 72.9% and 67.5%, respectively. Preferred areas were Seoul/Gyeonggi-do(53.5%) and Gangwon-do(38.8%). 'Stress solving and refreshment', 'taking a forest bath and a walk', and 'maintaining and promoting health' were the main purposes of the use. As for a therapy, 'walking therapy' was most preferred, and 'ergotherapy' was the next. First priority as for a use facility was 'healing trail', and 'professional medical facility' ranked second. Although important decision attributes were ' cost of use', 'food', and 'friendliness of medical staff', all the other sets of attributes related to use convenience, quality of medical service and tourism activities also recorded high, which forecasts higher consumer expectation for the product. As the result showing differences in consumer preference by the demographic segmentation, differentiated and segmented consumer needs should be considered when planing and managing a product. The scope of the study is limited to a demographic segmentation which is a basic stage of understanding consumer preference, therefore more detailed future researches on complicated and multi-dimensional consumer needs are required.

• Journal of Korean Home Economics Education Association
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• v.27 no.4
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• pp.67-91
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• 2015

The purpose of the present study is to analyze thematically everyday lives (food, clothing, shelter, spending, and leisure) of single women in their thirties or forties. As a result of the analysis, everyday lives of single women could be grouped into five main categories of themes which may be labeled as 'Self', 'Relationship', 'Survival', 'Preparation for the future', 'Freedom and hidden side of the freedom'. As for the first category of themes 'Self', three sub-themes have been found including "Only for me", "Perfection of self", "My own style". Next, as for the second category 'Relationship', two sub-themes have been found including "With her family (For her family, still under the protection of her family)", "With other people (Pleasures with people, Comfortable with single friends, Communicating with the world)". As for the third category 'Survival', three sub-themes have been found including "Health", "Safety", "Practice of living alone", As for the fourth category 'Preparation for the future', three sub-themes have been found including, 'Good spending', 'Savings for later life', 'Leisure in later life'. And finally, as for the fifth category 'Freedom and hidden side of the freedom', two sub-themes have been identified including "I am free because I'm alone (Freedom from bondage of my family, Compensation for being alone)", "Hidden side of the freedom (Fear, dislike feeling and insecurities due to being alone, Overcoming loneliness or solitude)". In all, there are eighteen sub-themes to consider. On the basis of these results, we presented some conclusions on the Everyday Lives of Single Women in their thirties or forties. We also presented some implications of these results on population education and future research.

• Journal of Life Science
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• v.25 no.8
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• pp.917-924
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• 2015

This study aimed to evaluate the protective effect of Orostachys malacophyllus (OM) and fermented O. malacophyllus (FOM) in Sprague-Dawley rats who had been intoxicated with 1% (w/w) orotic acid (OA) for 10 days. The activities of several hepatic enzymes, aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), and cholinesterase, were increased when OA was given, but these parameters were significantly decreased by FOM treatment. In addition, OA treatment resulted in an increased lipid peroxidative index (thiobarbituric acid-reactive substances, TBARS). A worsened antioxidant status (reduced glutathione) in the liver and serum was also observed. FOM treatment improved the antioxidant status of OA-induced fatty-liver rats, which was evaluated by decreased levels of the lipid peroxidative index and improved antioxidant status in the liver and serum. The contents of liver non-heme iron were increased with OA treatment and significantly decreased with FOM treatment, which suggested that the lipid peroxidation contents were inversely correlated with liver non-heme iron content. Based on these results, FOM is considered a material with significant potential for development into a functional health food that can improve fatty-liver conditions.

• Parasites, Hosts and Diseases
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• v.54 no.2
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• pp.181-185
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• 2016

Human sparganosis is a zoonotic disease caused by infection with larval forms (procercoid/plerocercoid) of Spirometra spp. The purpose of this study was to identify Spirometra spp. of infected snakes using a multiplex PCR assay and phylogenetic analysis of mitochondrial DNA sequence data from the spargana of terrestrial snakes obtained from Korea and China. A total of 283 snakes were obtained that included 4 species of Colubridae comprising Rhabdophis tigrinus tigrinus (n=150), Dinodon rufozonatum rufozonatum (n=64), Elaphe davidi (n=2), and Elaphe schrenkii (n=7), and 1 species of Viperidae, Agkistrodon saxatilis (n=60). The snakes were collected from the provinces of Chungbuk, Chungnam, and Gyeongbuk in Korea (n=161), and from China (n=122). The overall infection rate with spargana was 83% (235/283). The highest was recorded for D. rufozonatum rufozonatum (100%), followed by A. saxatilis (85%) and R. tigrinus tigrinus (80%), with a negative result for E. davidi (0%) and E. schrenkii (0%). The sequence identities between the spargana from snakes (n=50) and Spirometra erinaceieuropaei (KJ599680) or S. decipiens (KJ599679) control specimens were 90.8% and 99.2%, respectively. Pairwise genetic distances between spargana (n=50) and S. decipiens ranged from 0.0080 to 0.0107, while those between spargana and S. erinaceieuropaei ranged from 0.1070 to 0.1096. In this study, all of the 904 spargana analyzed were identified as S. decipiens either by a multiplex PCR assay (n=854) or mitochondrial cox1 sequence analysis (n=50).

• Journal of dental hygiene science
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• v.11 no.4
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• pp.325-332
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• 2011

To get the evidences for decreasing the discomfort, dissatisfaction, and for improving management of the implant prothesis through assessment implant patients' awareness and discomforts about care of the prostheses. The subjects were 300 implant patients who treated in the 20 dental clinics in Pusan, Ulsan and Daegu. The general characteristics, recognition, maintenance, discomfort and dissatisfaction, and oral hygiene status were collected. Level of the discomfort and dissatisfaction was measured by a 5 Likert scale, and analyzed by the equivalent of 100. The life-span of the implants was overestimated, however, periodic recall check and scaling were underwent. The level of discomfort and dissatisfaction was generally good, but many of them (65.3%) complained the food impaction. As a result of the multiple regression analysis, level of the discomfort and dissatisfaction increased in case of more number of the prosthesis ($\beta$=0.864), however decreased in case of more frequent teeth brushing ($\beta$=0.737), more frequent periodic check ($\beta$=-0.737), longer duration from the implantation ($\beta$=-0.090). It is very important for the implant patients to be recognized that the lack of care may shorten the life-span of the implant prosthesis. Therefore, efforts of the continuous oral hygiene care such as, periodic recall check, scaling, teeth brushing, the correct using of additional oral hygiene product, and education would be more emphasized.

• Journal of Life Science
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• v.21 no.10
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• pp.1355-1363
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• 2011

Many clinical trials have demonstrated the beneficial effects of garlic (Allium sativum) on general cardiovascular health. Aged garlic extract (AGE) is known to display diverse biological activities such as in antioxidant, anti-inflammatory and anticancer activities. However, few studies have been directed on the effect of AGE on cardiovascular function. In this study, we aimed to investigate the effect of AGE and its components on platelet activation, a key contributor in thrombotic diseases. In freshly isolated rat platelets, AGE and its components have shown inhibitory activities on thrombin-induced platelet aggregation. These in vitro results were further confirmed in an in vivo platelet aggregation measurement where tail vein injection of garlic oil and S-Allylmercapto-cysteine (SAMC) significantly reduced thrombin and ADP-induced platelet aggregation. Potential active components for antiplatelet effects of AGE were identified to be SAMC and diallyl sulphide through agonist-induced platelet aggregation assay. These results indicate that aged garlic extract can be a novel dietary supplement for the prevention of cardiovascular risks and the improvement of blood circulation.

• Journal of Life Science
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• v.22 no.2
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• pp.232-238
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• 2012

Kumjungsansung-Makgeolli$^{(R)}$ is a traditional Korean rice wine that is fermented from traditional nuruk and rice. In this study, we performed the PCR-denaturing gradient gel electrophoresis (DGGE) analysis targeting the 16S and 28S rRNA genes to characterize bacterial and fungal diversity during Makgeolli fermentation. The predominant bacteria in the PCR-DGGE profile during Makgeolli fermentation were Lactobacillus spp. (Lactobacillus curvatus, L. kisonensis, L. plantarum, L. sakei, and L. gasseri), Pediococcus spp. (P. acidilactici, P. parvulus, P. agglomerans, and P. pentosaceus), Pantoea spp. (P. agglomerans and P. ananatis), and Citrobacter freundii; these were identified on the base of analysis of 16S rRNA gene sequences. The dominant bacterium during Makgeolli fermentation was L. curvatus. The predominant fungi in PCR-DGGE profile during Makgeolli fermentation were Pichia kudriavzevii, Saccharomyces cerevisiae, Asidia idahoensis, Kluyveromyces marxianus, Saccharomycopsis fibuligera, and Torulaspora delbrueckii, and these were identified on the basis of analysis of 28S rRNA gene sequences. The dominant fungal species during Makgeolli fermentation changed from P. kudriavzevii at 0-2 days incubation to S. cerevisiae at 3-6 days incubation. This study suggests that PCR-DGGE analysis could be a suitable tool for the understanding of microbial diversity and structure during Makgeolli fermentation.