• Title/Summary/Keyword: Health/functional food

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Chemical Properties and Nitrite Scavenging and Acetylcholinesterase Inhibitory Activities from Salicornia herbaciea Seed (함초 씨앗의 화학적 특성과 아질산염 소거능 및 아세틸콜린에스터레이스 저해 효과)

  • Lim, Geum-Sook;Kim, Ran;Jeon, Kyung-Mi;Choi, Hyun-Suk;Cho, Hoon;Koh, Ha-Young;Choi, Chang-Nam
    • KSBB Journal
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    • v.28 no.6
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    • pp.372-379
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    • 2013
  • This study was to investigate the chemical properties and nitrite scavenging and acetylcholinesterase inhibitory activities from Salicornia herbaciea seed. The lactic acid content of seed was about 2.0 fold higher than that of stem. Among various free sugars, the maximum fructose of seed, glucose of stem, and mannose contents of root were obtained, 176.3, 125.6, and 112.8 mg/100g, respectively. The maximum leucine content of seed among the essential amino acid was obtained, 853.7 mg/100g, which was about 3.0 or 6.0 folds higher than that of root or stem. In the case of glutamic acid of seed, it was 2,388.7 mg/100g, which was 5.6 or 9.8 folds higher han that of root or stem. The ratio of essential amino acid and total amino acid of seed was 30.14%. The ${\gamma}$-aminobutyric acid contents of seed, stem, and root were 43.87, 23.88, and 27.8 mg/100g, respectively. The catechin content of seed was an order of epigallocatechin (723.2 mg/100g) > epigallocatechingallate (654.3 mg/100g) > epicatechin (443.5 mg/100g) > gallocatechin (314.1 mg/100g). Especially, the non-gallated catechins content was about 2.0 folds higher than that of gallated catechins content. The nitrite scavenging activity of seed increased from 38.7 to 65.9% when the hot-water extract content of seed at pH 1.2 increased from 1.0 to 5.0 mg/mL. However, it was decreased to 25.7% at pH 6. The acetylcholinesterase inhibitory activity of seed was increased from 13.2 to 44.6% when the extract content increased from 20 to 100 mg/mL. These results show that S. herbaciea seed has a good potential to be used as a source of material or additive in cosmetics, food, and drug compositions.

Functional Properties of Mugwort Extracts and Quality Characteristics of Noodles Added Mugwort Powder (쑥 추출물의 기능성과 쑥국수의 품질특성)

  • Park Chan-Sung;Kim Mi-Lim
    • Food Science and Preservation
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    • v.13 no.2
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    • pp.161-167
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    • 2006
  • This study was investigated to develope health promoting and high quality of mugwort noodle. Mugwort powder was extracted with water and 70% ethanol and the extracts were tested it electron donating ability (EDA), nitrite scavenging ability (NSA) and inhibitory effects on MDA cell and A549 cell. EDA at 100-1,000 ppm of water extract was ranged f개m 73% to 81% and that of ethanol extract was ranged from 74% to 92% NSA of water extract was 40% and ethanol extract was 41% at 1,000 ppm, which were the highest at pH 1.2. NSA was increased with increasing conncentration of mugwort extracts and decreasing pH. Inhibition ratio of water and ethanol extracts on MDA cell growth was 30 and 27% while that on A549 cell was 22% and 23% at 1,000 ppm, respectively. Quality characteristics of mugwort none were evaluated by their color, shelf life na sensory characteristics. Lightness (L) and redness(a) of dried noodle and cooked noodle were decreased with increasing mugwort concentration(p<0.05). The number of total viable cells and fungi in mugwort noodle was $0.5{\sim}0.7log$ cycles lower than that of contro. In sensory evaluation of dried noodles and cooked noodles, noodles with 2% mugwort powder had significant high scores in overall acceptability.

Changes in Nutrient Levels of Aqueous Extracts from Radish (Raphanus sativus L.) Root during Liquefaction by Heat and Non-heat Processing

  • Bae, Ro-Na;Lee, Young-Kyu;Lee, Seung-Koo
    • Horticultural Science & Technology
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    • v.30 no.4
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    • pp.409-416
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    • 2012
  • The amount of cellular components including soluble sugars, amino acids, organic acids and glucosinolates (GLS) was investigated during radish root processing to develop a radish beverage. The radish root was divided into two parts, white and green tissue, and processed separately by extracting the juice from the fresh tissue and from the boiled tissue to compare differences in the components content among the preparations. The overall palatability of both the fresh and boiled extracts from the green part of the radish was higher than that of the same extracts from the white part. The sweetness of extract by boiling increased and its pungency decreased, thereby the palatability increased by being compared to the fresh radish extract. The sweetness was affected by sucrose not by glucose or fructose of monosaccharides by showing different sucrose contents according to treatment comparing palatability. Malic acid was identified as primary organic acid, and the content was higher in both the fresh and boiled extracts from the white part than in the extracts from the green part of the radish. The fresh extract from the green part of the radish contained more essential amino acids, such as threonine and valine, and more hydrophilic amino acids including glutamic acid, aspartic acid, and arginine than those of the fresh extract from the white part, suggesting the green fresh part is more palatable than the white fresh part. The main sulfur compound was ethylthiocyanate in radish, and others were butyl isothiocyanate, dimethyl-disulfide, and 4-methylthio-3-butylisothiocyanate. The four GLS were detected much more in the fresh green and fresh white parts of the radish because they evaporated during boiling. The contents of the four sulfur compounds were higher in the white fresh part than in the green fresh part, which is likely the reason the pungency was higher and the palatability was lower in the white fresh part than in the green fresh part of the radish. The ascorbic acid content was higher in the fresh extract compared to the boiled extracts from both the green and white parts. Taken together, these findings indicate that fresh radish extract is superior to obtain in terms of retaining desirable nutritional and functional components for health.

Antioxidant Enzymes and Antimicrobial Activities in Sponge Gourds (수세미 오이의 항산화 효소 및 항균 활성 검증)

  • Lee, Hee Ju;Moon, Ji Hye;Lee, Woo Moon;Lee, Sang Gyu;Park, Dong Kum;Yoon, Moo Kyung
    • Horticultural Science & Technology
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    • v.32 no.5
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    • pp.702-709
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    • 2014
  • Luffa cylindrica Roem (sponge gourd) belongs to the Cucurbitaceae family and has been cultivated as an ornamental plant in Korea. Recently, its cultivation area has been increased with an increase in demand for it as an ingredient in cosmetics, herbal medicines, and health supplements. We analyzed inorganic components of sap collected from land race sponge gourds. We also measured antioxidant enzyme activities and antimicrobial activities of the plant, seed, and sap to examine functional properties of sponge gourd. The sap of the sponge gourd contained high levels of K, Ca, P, and Mg, with the most abundant mineral in the sap being K ($470mg{\cdot}L^{-1}$). The amounts of Ca and Mg were 2 and 1.7 times more than those found in cucumber (Cucumis sativus), respectively. Ascorbate peroxidase was more active than catalase and superoxide dismutase in various plant parts of sponge gourd. Antioxidant activities were much lower in stems than in other plant parts such as leaves, roots, flowers, fruits, seeds, and sap. In addition, sap showed a very low level of antimicrobial activity against two food-borne pathogens, Vibrio parahaemdyticus and Propionibacterium acne, and none against the other eight tested food-borne pathogens. Antimicrobial activities against Candida albicans and Malassezia furfur, which causes dermatitis, appeared to be higher in sap than in other parts of sponge gourd plants. Overall, the antimicrobial activity against Malassezia furfur appeared to be higher than against Candida albicans.

Development of Probiotic Dairy Product for the Normalization of Microbial Flora in Korean Infants (한국인 영유아 장내균총 정상화를 위한 프로바이오틱 유제품의 개발)

  • Kim, Min-Kyung;Choi, A-Ri;Han, Gi-Sung;Jeong, Seok-Geun;Chae, Hyun-Seok;Jang, Ae-Ra;Seol, Kuk-Hwan;Oh, Mi-Hwa;Kim, Dong-Hun;Ham, Jun-Sang
    • Food Science of Animal Resources
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    • v.31 no.2
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    • pp.290-295
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    • 2011
  • This study was conducted to develop an effective probiotic dairy product to normalize the microbial flora in Korean infants. A total of 2,200 colonies were isolated from 25 Korean neonates, and 16S rRNA of 348 isolates was analyzed. Approximately 40% of the lactic-acid producing bacterial isolates were Enterococcus faecalis, and 34.2% of them were strains similar to XR7 in the GenBank database. The fastest growing strain in MRS broth was registered as 91532 by the KACC. The selected strain was freeze-dried and utilized to ferment a milk-containing rice soup, tarakjuk. Microbiological, physico-chemical, and sensory characteristics of the fermented tarakjuk were compared with fermented milk and tarakjuk. E. faecalis KACC 91532 increased from $6.14{\pm}0.19$ to $7.36{\pm}0.13$ Log CFU/mL, and can be useful as a probiotic, as described in the Standards for Functional Health Foods.

Effect of Fermented Garlic Extract on Adhesion of THP-1 Cells to Bovine Aortic Endothelial Cells (혈관내피세포에 부착하는 THP-1에 대한 발효마늘추출물의 효과)

  • Kwon, Ohseong;Song, Jina;Yu, Heui Jong;Park, Heonyong
    • Journal of Life Science
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    • v.27 no.5
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    • pp.553-560
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    • 2017
  • Fermented garlic extract (FGE) is a well-known functional food containing cycloalliin. Here we tested if FGE has an effect on the vascular system. We found that FGE had an effect on monocytic adhesion to endothelial cells, which occurs at the initial step of processes of inflammation. From dose-dependent experiments, 0.1 to $1{\mu}g/ml$ of FGE appeared to inhibit lipopolysaccharide (LPS)-enhanced heterotypic cell adhesion between THP-1 and BAECs. This result indicates that FGE blocks vascular inflammation. Then we found that FGE activates eNOS and Akt in BAECs. The phosphorylation of eNOS was maximally elevated 10 min after FGE treatment. Parallely, the phosphorylation of Akt was also maximally increased 10 min after FGE treatment. Consistently, it was found that FGE enhanced the production of nitric oxide. We then examined whether NO mediates THP-1 cell adhesion to BAECs. Both Akt and eNOS inhibitors appeared to reverse an inhibitory effect of FGE. These findings indicate that FGE inhibits LPS-enhanced heterotypic cell adhesion via Akt and eNOS. In conclusion, FGE plays an important role in prevention of inflammatory diseases.

Improvement of High-fat Diet-induced Obesity by Xanthigen in C57BL/6N Mice (잔티젠(Xanthigen)에 의한 고지방식이로 유도된 비만 개선 효과)

  • Choi, Kyeong-Mi;Lee, Youn-Sun;Kim, Wonkyun;Choi, Yung-Hyun;Kwak, Youn-Gil;Jung, Jae-Chul;Lee, Jeongrai;Yoo, Hwan-Soo
    • Journal of Life Science
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    • v.22 no.12
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    • pp.1697-1703
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    • 2012
  • Obesity is a risk factor for numerous metabolic diseases. Recently, naturally occurring compounds that may improve obesity have received increasing attention. Xanthigen is a mixture of fucoxanthin and punicic acid derived from brown seaweed and pomegranate seed, respectively, which have been traditionally used for lipid-lowering effects in humans. In this study, we investigated whether Xanthigen attenuates high-fat diet-induced obesity in C57BL/6N mice. The mice were fed on a normal diet (ND), high-fat diet (HFD), HFD plus 1% Xanthigen or HFD plus 1% green tea extract (GTE) for 11 weeks. Food efficiency ratio (FER) and body weight were significantly reduced in mice fed HFD plus Xanthigen compared to HFD-fed mice. Consistent with the results in body weight change, Xanthigen also significantly decreased the weights of epididymal adipose tissue, retroperitoneal adipose tissue, and liver in HFD plus 1% Xanthigen-fed mice. The serum level of low-density lipoprotein (LDL)-cholesterol was significantly decreased in HFD plus Xanthigen-fed mice compared to HFD-fed mice. These results suggest that Xanthigen may be useful in the development of a functional health food for anti-obesity.

Mass Production of Chaff-vinegar and Its Effect of Anti-Aging and Whitening (왕겨초액의 대량생산과 항노화 및 미백 효과)

  • Hwang, Jaegyu;Yun, Jong-Kuk;Kim, Suk-Kyung;Lee, Sang-Han;Han, Kil-Hwan
    • Microbiology and Biotechnology Letters
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    • v.40 no.3
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    • pp.208-214
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    • 2012
  • Chaff-vinegar is known for having a variety of useful purposes in the fields of health and lifestyles. In a previous study we isolated and identified the active fractions of the polyphenol compound 7 species as a potential biomaterial for cosmeceuticals. To further test for its potential use as a functional material, we carried out an MTT assay, collagenase inhibition assay, elastase inhibition assay, tyrosinase inhibition assay, DPPH free radical scavenging assay, SOD-like activity assay and a xanthine oxidase inhibition assay. Chaff-vinegar exhibited potent collagenase and elastase inhibitory activities in a concentration dependent manner, indicating that the agent has the potential to alleviate the skin wrinkling process. Chaff-vinegar also showed 80% tyrosinase inhibition at a concentration of $100{\mu}L/mL$. DPPH radical scavenging, xanthine oxidase inhibition, and SOD-like activity results for each activity were 80%, 80%, and 100%, respectively. Taken together, the present study suggests that chaff-vinegar is a good candidate for use as an anti-wrinkling and/or whitening agent.

Functional Properties of Angelica gigas Nakai Leave (AGL)Extracts and Quality Characteristics of Mumalangi Kimchi Added AGL (당귀잎 추출물의 기능성과 당귀잎을 첨가한 무말랭이 김치의 품질특성)

  • Kim, Mi-Lim;Park, Chan-Sung
    • Korean journal of food and cookery science
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    • v.22 no.5 s.95
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    • pp.728-735
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    • 2006
  • This study investigated the development of health promoting high quality Mumalangi Kimchi. Angelica gigas Nakai leaves (AGL) were extracted with water and 70% ethanol, and the extracts tested for their electron donating ability (EDA), nitrite scavenging ability (NSA) and inhibitory effects on MDA and A549 cells. The EDA in 100-1,000 ppm water extracts from AGL ranged from 40 to 80%, but that of the ethanol extracts ranged from 37 to 81%. The NSA increased with increasing AGLconcentration in the extracts and decreasing pH. The NSA of the 1,000 ppm water and ethanol extracts from AGL were 29 and 35%, respectively, at pH 1.2. The inhibition ratios of the water and ethanol extracts from AGL on MDA cell growth were 35 and 32%, while those on A549 cell growth were 27 and 23%, respectively, at 1,000 ppm. After sun drying radishes for 15 hours, for the preparation of Mumalangi, the water contents were higher in summer radishes (39.5%) than fall radishes (32.6%) the color of summer radish also changed to brown. During storage of Mumalangi Kimchi, with the addition of 1-3% AGL, at 20?for 4 weeks, the yeast growth was inhibited. The shelf-life of Mumalangi Kimchi was extended by the addition of AGL. In the sensory evaluation of Mumalangi Kimchi, that with the addition 2% AGL had the highest scores for color, flavor, taste, texture, after taste and overall acceptability. Mumalangi Kimchi with the addition of 2% AGL had significant high scores for both taste and overall acceptability (p.0.05).

Changes in Free Sugar, Coixol Contents and Antioxidant Activities of Adlay Sprout (Coix lacryma-jobi L. var. ma-yuen Stapf.) according to Different Growth Stage (생육시기 별 새싹율무의 유리당, 기능성 성분 및 항산화활성 변화)

  • Lee, Hee Jung;Lee, Jeong Hoon;Jung, Jin Tae;Lee, Yun Ji;Oh, Myeong Won;Chang, Jae Ki;Jeong, Heon Sang;Park, Chun Geon
    • Korean Journal of Medicinal Crop Science
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    • v.27 no.5
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    • pp.339-347
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    • 2019
  • Background: Adlay (Coix lacryma-jobi L. var. ma-yuen Stapf.) is an important medicinal and cereal crop that contains high levels of protein, fatty acids and crude fiber, as well as calcium, phosphorus, zinc, minerals, and essential amino acids. Despite its economic significance, little is known about biological activity in adlay sprouts. This study investigated the total free sugar, polyphenol and flavonoid content, and antioxidative activity in adlay sprouts at different growth stages. Methods and Results: The total free sugar and coixol content of germinated adlay sprouts were analyzed by high performance liquid chromatography. The total phenolic content was measured by the Folin and Denis, and flavonoid content by the Davis method. Antioxidative activity was tested by the 2,2-diphenyl-1-picryl hydrazyl method. All measured component, including total phenolic and coixol content ($2.71{\pm}0.02mg/g$ and $59.70{\pm}0.01mg/g$), and antioxidant capacity ($IC_{50}$; $453.93{\mu}g/m{\ell}$) were highest in 3 day old adlay sprouts. In contrast, total free sugar and flavonoid content (3.02% and $0.60{\pm}0.02mg/g$) were highest in sprouts at 5 days after sowing. In addition, there was a strong positive correlation between antioxidative activity and total polyphenol content (r = 0.902, p < 0.001). Conclusions: Adlay sprouts between 3 days and 5 days following germination contain high levels of functional components and free sugar. This study therefore suggests that adlay sprouts can be developed as a valuable health-promoting food and a good source of natural antioxidants.