• 한국발생생물학회지:발생과생식
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• 제18권3호
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• pp.179-186
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• 2014

Characteristics of the developmental stages of spermatids during spermiogenesis and phylogenetic classicfication of the species using sperm ultrastructures in male Crassostrea ariakensis were investigated by transmission electron microscope observations. The morphology of the spermatozoon of this species has a primitive type and is similar to those of Ostreidae. Ultrastructures of mature sperms are composed of broad, modified cap-shaped acrosomal vesicle and an axial rod in subacrosomal materials on an oval nucleus, four spherical mitochondria in the sperm midpiece, and satellite fibres which appear near the distal centriole. The axoneme of the sperm tail shows a 9+2 structure. Accordingly, the ultrastructural characteristics of mature sperm of C. ariakensis resemble to those of other investigated ostreids in Ostreidae in the subclass Pteriomorphia. In this study, particularly, two transverse bands (stripes) appear at the anterior region of the acrosomal vesicle of this species, unlike two or three transverse bands (stripes) in C. gigas. It is assumed that differences in this acrosomal substructure are associated with the inability of fertilization between the genus Crassostrea and other genus species in Ostreidae. Therefore, we can use sperm ultrastructures and morphologies in the resolution of taxonomic relationships within the Ostreidae in the subclass Pteriomorphia. These spermatozoa, which contain several ultrastructures such as acrosomal vesicle, an axial rod in the sperm head part and four mitochondria and satellite fibres in the sperm midpiece, belong to the family Ostreidae in the subclass Pteriomorphia.

• 대한수의학회지
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• 제39권5호
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• pp.896-907
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• 1999

The morphometrical characteristics such as external measurements and mandible size assessment in mice and rats have to be highly heritable and sufficiently variable between strains in order to calculate a strain specific profiles. The coat color of Korean wild rats were observed and morphometric analysis of external measurements were carried out on Korean wild rats compared to laboratory strains in order to clarify the genetic characteristics of Korean wild rats and to establish background data as a domestication of Korean wild rats for new laboratory strain. Korean wild rats were captured from Chunchon and Hoengsong. 4 inbred and 1 outbred strains of rats were used in this study for the comparison of genetic characteristic of Korean wild rats. Total body length, head length, tail length, hind foot length and ear length were measured and then statistical analysis were carried out by discrimiant analysis. The coat color of Korean wild rat showed golden white in ventral portion and dark agouti in dorsal portion. Korean wild rats could be distinguished from the other laboratory strains distinctly by morphogenetical analysis. There was significant variations among Korean wild rat compared to those of the other laboratory strains of rat. This study may provide that Korean wild rats have a unique genetic characterization compared to those of other inbred strains of rats based on morphogenetical characteristics by external measurements.

• 수산해양기술연구
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• 제25권4호
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• pp.191-199
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• 1989

본 연구에서는 이스라엘잉어 Cyprinus carpio를 체장과 체중별로 분류하여 방위별 표적강도를 측정한 후, 체장 및 체중의 변화에 따른 표적강도와의 관계를 고찰하였으며, 그 결과는 다음과 같다. 1. 이스라엘잉어를 대상으로 하여 측정된 방위별 표적강도는 부레의 위치변화에 따라 심한 변화폭을 보였으나, 대체적으로 최대표적강도의 방위별 분포는 dorsal aspect인 경우 등쪽을 기준하여 $10^{\circ}$ 정도의 방위변화에서 최대였으며, side aspect인 경우 음원의 수직 측면상의 5$^{\circ}$~$10^{\circ}$에서 최대였다. 2. 이스라엘잉어에 대한 체장, 체중별 표적강도는 체장과 체중에 비례하여 지수함수적으로 증가하였으며, 체장(L) 및 체중(W)의 변화에 따른 표적강도의 관계식은 다음과 같다. TS sub(L)=20 LogL-65.4. TS sub(W)=6.7 Log W-53.7. TS sub(L): 체장에 대한 표적강도(dB). TS sub(W): 체중에 대한 표적강도(dB).

• 대한기계학회논문집A
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• 제37권9호
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• pp.1117-1125
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• 2013

후판압연 중에 발생하는 소재의 평면형상과 손실량을 정확하게 고찰할 수 있는 유한요소에 기초한 접근법을 제시한다. 압연의 패스 수가 증가함에 따른 소재의 선, 후단부의 형상을 연속적으로 모사할 수 있는 3 차원 유한요소모델을 도입하였다. 본 모델의 가장 큰 특징은 다단 압연을 할 수 있도록 롤 갭이 순차적으로 감소됨에 따라 소재의 압연 방향을 바꾸면서 중단 없이 해석 가능하다는데 있다. 유한요소해석에서 요구되는 재료상수는 열간 인장시험에 의해서 실험적으로 얻어졌다. 제시된 유한요소모델의 유용성을 입증하기 위해서 파이롯트 열간 판압연 시험을 실시하였다. 제시된 유한요소모델로 계산된 평면형상과 손실량이 판압연 시험에서 실제 측정된 양과 매우 잘 일치하는 결과를 얻었다. 제시된 모델로 예측한 손실량과 판압연 시험에서 측정한 양 사이의 오차는 5% 이하로 나타났다.

• Asian-Australasian Journal of Animal Sciences
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• 제14권2호
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• pp.280-287
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• 2001

These studies aimed to determine how lighting might be used to improve feed intake and reduce aggressive behavior in newly weaned pigs. To examine whether this objective could be achieved an experiment was conducted to compare performance, behavior and body condition of weaners over 17-45 days, provided similar lighting quality (i.e. color temperature, color rendering index and lighting distribution) after weaning that piglets experienced prior to weaning. Triphosphor (TP) lighting to simulate daylight was provided during the day while at night, Pascal red (PR) lighting was provided to simulate the night-light piglets previously had received from infrared heating lamps. This treatment was compared to weaners provided conventional cool-white fluorescent light during the day only. Weaners on treatment lighting from 17-45 days of age showed no improvement in body weight or feed conversion at 24, 31, 38 and 45 days compared to the controls. There was, however, a significant improvement (p<0.05) in feed intake in the first week of weaning for weaners provided TP/PR lighting. Over the first 3 days of weaning, pigs on TP/PR lighting showed an increase (p<0.05) in the incidence of ear chewing but reduced (p<0.05) levels of nosing the abdomen of other pigs and reduced (p<0.05) occurrences of being stood on by other pigs. Females exhibited more (p<0.05) mounting and nosing behaviors and rubbing the heads of other pigs than males. On the other hand, males engaged in more (p<0.05) fighting, nipping, ear chewing and standing on other pigs compared to females. Pigs provided PR lighting on the first night of weaning engaged in higher (p<0.05) incidences of nosing and tail sucking behaviors, more (p<0.05) head thrusting, fighting and ear chewing compared to control pigs. The body condition of weaners provided the TP/PR lighting treatment was significantly poorer (p<0.05) compared to weaners on control lighting. In conclusion there was no improvement in production performance of weaners provided new technology lighting apart from the improvement in feed intake in the first week weaners were exposed to the TP/PR lighting.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 추계국제학술대회
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• pp.159-159
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• 2003

Comet assay is a potential monitoring tool because DNA strand breakage may be produced by a wide range of agents. The comet assay, also called the single-cell gell electrophoresis (SCGE) assay, is rapid and sensitive method for the detection of DNA damage in cells. This study was performed for the identification of DNA damage in the cells from flounders and clams exposed to PAHs. As a control experiments, flounder and clam cells were exposed to $H_2O$$_2$. The cells exposed to $H_2O$$_2$ were displayed a typical nuclei movement DNA damage of cells were significantly increased when the isolated cells from the blood of flounders and the tissue of clams were in vitro exposed to the different concentrations (5, 10, 50, 100 ppb) of five kinds of PAHs (benzo[a]pyrene, pyrene, fluoranthene, anthrancene, and phenanthrene). For the in vivo test, flounders and clams were exposed to the different concentrations of BaP for 4 days. The results showed that DNA strand breakage was effected by the concentration of BaP and the duration of exposure. In high concentration of BaP, the mean tail lengths of nuclei was longer than it In low concentration, while the mean size of head DNA decreased. In this research, both in vitro and in vivo genotoxicity of PAHs could be biomonitored by the comet assay. Especially, clams and flounders seem to be useful as materials for monitoring genotoxic damage by comet assay.

• 수산해양기술연구
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• 제36권1호
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• pp.1-11
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• 2000

A low frequency, high power hydroacoustic transducer with 7 tonpilz piston elements assembled in a circular array suitable for marine application, such as the transmission of underwater information and the development of new fisheries resources in the deep sea zone was designed. A modified Mason's model was applied to monitor and to simulate the transducer behavior at each step during the fabrication. The in air, and in water constructed tonpilz transducer was tested experimentally and numerically by changing the size and the type of the material for head, tail and acoustic window. Also, the developed transducer was excited by pulse signals and the received waveform was analyzed. The resonance peaks in the transmitting voltage response(TVR) of a single tonpilz element without housing were observed at 11.33kHz in air and 10.93kHz in air and 10.93 kHz in water, respectively, with the overall electrical-acoustic efficiency of 43.7%. The value of TVR of single tonpilz element with aluminum housing in water was 129.87dB re 1 $\mu$Pa/V at 12.25 kHz with the frequency bandwith of 2.15 kHz and half beam angle of 30.2$^{\circ}$at -3dB.The resonance peaks in the transmitting voltage response of the 7 element circular transducer were observed at 11.50 kHz in air and 11.45 kHz in water, respectively. The value of TVR in water 144.84 dB re 1$\mu$Pa/V at 11.5kHz with the frequency bandwith of 4.25 kHz and the half beam angle of $22.3^{\circ} $ at -3dB.Reasonable agreement between the experimental measurements and the theoretical predictions for the directivity patterns, TVRs and the impedance characteristics of the designed transducer was achieved.

• Applied Microscopy
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• 제41권3호
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• pp.179-188
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• 2011

성숙한 2년산 미꾸라지(Misgurnus mizolepis) 친어로부터 3배체를 유도하여 이들 3배체의 정자형성과정에 따른 생식세포의 미세구조를 조사하여 2배체 숫컷과 비교하였다. 3배체 숫컷의 정자형성과정과 세포학적 미세구조는 2배체와 유사한 특징을 나타내었다. 정자변태과정이 일어나는 시기에 3배체의 정소 소엽내 Sertoli cell은 세포질 내 다수의 미토콘드리아 로제트, 소포체, 그리고 소량의 지방적 등을 함유하는 등 높은 활성을 나타내었다. 3배체 숫컷의 정소에서 일부 성숙한 정자들이 식별되었다. 변태를 완료한 3배체의 정자는 두부, 중편 그리고 미부축사로 구성되어 2배체 정자와 같은 anacrosomal aquasperm으로 나타났으나 생식세포 발달단계별 핵의 크기가 2배체보다 크고, 정자를 구성하는 cytoplasmic cannal의 직경이 크며 cytoplasmic collar의길이가 길고 미부축사의 직경이 굵고 두 개 이상의 미부축사를 가지는 등 비정상적인 구조를 나타내었다.

• 한국수정란이식학회지
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• 제25권4호
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• pp.237-245
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• 2010

Only an optimum number of viable spermatozoa in a frozen-thawed insemination dose can ensure conception at artificial insemination (AI). We report here the percentages of normal, abnormal and viable spermatozoa present in the frozen-thawed semen of 20 Black Bengal bucks used for commercial AI. Bucks in this experiment were of 19.3~46.1 months old and 25~42 kg body weight. Four semen straws (0.25 ml) from each buck were collected for evaluation of their kinetic parameters. Scrotal circumference was measured by using a scrotal tape, sperm motility was estimated on eye estimation and sperm concentration was determined by using a haemocytometer. Sperm morphology was studied in paraformaldehyde fixed spermatozoa under differential interference contrast (DIC) microscope. To determine the proportion of live (plasma membrane intact) spermatozoa, semen was stained with SYBR-14 and propidium iodide and examined under fluorescent microscope. Scrotal circumference, post-thaw sperm motility, sperm concentration per insemination dose and proportion of normal spermatozoa were $21.5{\pm}0.7\;cm$, $43.5 {\pm}5.4%$, $83.5{\pm}6.7$ million and $88.3{\pm}4.1%$, respectively. The percentages of spermatozoa with head shape and acrosome abnormalities were lower ($2.7{\pm}1.1$ and $1.4{\pm}1.3$, respectively), whereas higher percentages of abnormalities ($7.0{\pm}1.8$) were observed in mid piece and tail portion. The proportion of live spermatozoa was $28.5{\pm}5.4$. It is concluded that although a good number of morphologically normal spermatozoa are present in the insemination dose, the proportion of live spermatozoa is low, which warrants further improvements of buck semen freezing procedures to ensure good quality at AI.

• 한국철도학회논문집
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• 제16권6호
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• pp.454-459
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• 2013

열차가 터널에 고속으로 진입하면, 압력파가 발생한다. 열차 선두부의 진입에 의하여 발생한 압축파는 터널을 따라 진행되어 터널 출구에서 반사되어 팽창파로 되돌아오며, 후미부의 진입에 의하여 발생한 팽창파도 터널을 따라 전파되어 터널 출구에서 압축파로 반사되어 터널 입구로 되돌아 온다. 열차 선두부 및 후미부에 의하여 발생한 이러한 압력파는 터널 입구 및 출구에서 각각 반사되어 터널 내부를 왕복하며, 차량 객실에 탑승한 승객들에게는 이명감을 일으키고, 터널 출구에서는 환경소음의 일종인 미기압파를 발생시킨다. 터널에서의 큰 압력 변동은 터널의 최적 단면적 설계에도 주요 인자로 고려되고 있으며, 차체의 반복 피로 하중으로 작용하므로, 이에 대한 정량적 및 정성적 분석이 필요하다. 본 연구에서는 고정 격자계를 이용하여 개발한 특성 해법을 교행하는 열차에 대하여 적용하였으며, 교행시의 열차 선두부 및 후미부의 경계 조건식을 개발하여, X-t선도와 같이 해석하였다. 해석 결과, 교행 열차의 특정 터널진입 시간에 압력파 간의 상쇄가 일어남을 알 수 있었다.