• Applied Microscopy
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• v.28 no.4
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• pp.447-463
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• 1998

This study was carried out to investigate the histomorphological changes and the electrophoretic patterns of egg components, obtained from 100 of 2-year-old female catfishes (Silurus asotus). Female Korean catfishes collected in the vicinity of Chollabukdo have synchronous ovaries which discontinously ovulate eggs during the breeding season (from late May until early July). The fishes were killed by severing the spinal cord just posterior to the head after immobilization with tricaine methanesulfonate (MS 222). Especially, the light microscopic and ultrastructural changes of ooplasm and follicular membranes of oocytes, were examined by means of light and transmission electron microscopy. The size of the nucleoli and number of the yolk granules increased as the oocyte groved. Yolk granules were deposited in the oocyte as fluid Due to tile presence of large early and late maturing oocytes, their ovaries were enlarged, transparent, granular and greenish in color. As the percentages of fish in late maturing oocyte (LMO) and ripe oocyte (RO) stage increased from March to April, mean gonadosomatic index (GSI) values (19.95%) increased. Zona radiata changed from squamous into cuboid in shape in the early maturing oocyte (EMO) stage. Processes, microvilli, from the zona radiata and from the oocyte grow, and make contact with each other in the pore canals of the zona radiata during vitellogenesis, but are withdrawn as the zona radials becomes more compact and devoid of pore canals during oocyte maturation. Seasonal changes in the microscopic appearance of the ovaries were well correlated with those in both gonadosomatic index and macroscopic appearance. The main cytological changes such as increase in size of cell, nucleus, nucleolus, and increase in number of nucleoli and mitochondria demonstrated with electron microscopy in the previtellogenic oocytes of Korean catfish, provided evidences for important synthetic processes in an early preparatory phase of oocyte development. The electrophoretic pattern of major band in mature stage was much thicker (24 k, 66 k, 90-110k dalton) than that in previtellogenic phase.

• Applied Microscopy
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• v.28 no.2
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• pp.193-205
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• 1998

The Urechis unicinctus sperm and spermatogenic cells prepared from the testis are investigated to identify $\alpha-tubulin$ of axoneme microtubules using mouse monoclonal $anti-\alpha-tubulin$ as the first Ab and Gold(10nm) conjugated goat anti-mouse IgG as the Ab marker. The Ag-Ab reaction analyzed excellently the localization of $\alpha-tubulin$ and the gold particles incorporated with the proximal and distal centrioles, manchette microtubules, and flagellum. The gold particles can be also observed in the spermatogenic cells while the cells are still in sperm ball which is composed of a somatic cell and spermatogenic cells. The sperm ball is the functional unit of sperm production in U unicinctus testis. The spermatids are developed from the spermatogenic cells in the sperm ball and released into the testis cavity through a cortical cytoplasmic opening. The spermatid architectures are similar with the mature sperm of the testis cavity in aspects of shape of discoid acrosome, degree of nuclear condensation and ring type of mitochondrion. However, the distal centriole connecting with the flagella can be observed from the mature sperm while the both proximal and distal centrioles reveal only in the spermatids. The proximal centriole is directly connected with nuclear outer membrane during the stage of nuclear condensation and oriented perpendicularly to the distal centriole whose axis coinciding with the longitudinal axis of the spermatozoon. There are indications that the distal centriole is intimately associated with the polymerization of the flagellum. The manchette microtubules appear during spermatid development but the mature sperm have round head and no conspicuous middle piece.

• Transactions of the Korean Society of Automotive Engineers
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• v.14 no.5
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• pp.155-163
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• 2006

Car seat is one the most important element to make comfortable drivability. It can absorb the impact or vibration during driving state. In addition to those factors, it is needed to have enough strength for passenger safety. From energy efficiency and environmental point of view lighter passenger car seat frame becomes hot issue in the auto industry. In this paper, weight optimization methodology is investigated for commercial car seat frame using CAE. Optimized designs for seat frame are developed using commercially available finite element code(ANSYS) and design of experiment method. At first, car seat frame is modelled using 3-D computer aided design tool(CATIA) and simplified for finite element modelling. Finite element analysis is carried out for the case of FMVSS 202 Head Restraint test to check the strength of the original seat frame. Two base brackets are selected as optimized elements that are the heaviest parts in the seat frame. After finite element analysis for the brackets with similar load condition to the previous test optimization technique is applied for 10% to 50% weight reduction. Design of experiment is utilized to obtain optimization design for the bracket based on the modified 50% weight reduction model in which outer shape of the bracket is conserved. Weight optimization models result in the decrease of the strength in spite of weight reduction. The more design points should be considered to get better optimized model. The more advanced optimization technique may be utilized for more parts of the seat frame to increase whole seat frame characteristics in the future.

• The Korean Journal of Pesticide Science
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• v.9 no.4
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• pp.354-358
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• 2005

The study was carried out to investigate dissipation pattern of pesticide residue in/on different varieties of lettuce applied with foliar spraying under greenhouse. The initial deposited amount of alpha-cypermethrin and imidacloprid in/on the crop was entirely corresponded with shape of the crop. Dissipation of deposited pesticide residue was supposed to be related with degradation by sunlight and wash off by watering rather than dilution effect by biomass increase. The crop grouping in leaf vegetables has to be carefully considered even in lettuce for dissipation pattern of pesticide residue; head type and leafy type.

• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.5
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• pp.1089-1101
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• 2006

Following conclusions are drawn from study about Yukmigiwhangwon through referring to literatures and reviewing in the point of Hyungsang medicine. Yukmigiwhangwon was prescribed by Jeon Eul in order to cure five kinds of flaccidity of infants, but after since then it has been basically applied to symptoms which was caused by deficiency of the kidney not only infants but also adult. The deficiency of kidney is the lack of vital essence and body fluid stored in kidney, it happens because infants are born in the conditions of lack of receiving in the womb. And it happens because adults infringe the rules of life, especially the sexual life. Main symptoms of the poverty of renal functions are emptiness of the brain marrow, dizziness, forgetfulness, tinnitus, deafness, vertigo and dim eyesight, insomnia, the low back and knees aching and limping, heat on the legs, tibia aching, nocturnal emission, dry mouth, reddened tongue and little coating or non coating of the 'tongue, weak blood meridians. the treating method is nourishing Eum and supplementing kidney. In Dongeuibogam Yukmigiwhangwon is mentioned in the parts of vital essence, blood, voice, kidney, urine, eye, ear, the low back, hair, fire, exhaustion syndrome, cough, polydipsia, infant, etc. The shapes which Yukmigiwhangwon is applied to are the Dam Body Doing deficiency of Eum due to excess of Yang, man, infant, Jung type, Fish group, reverse triangular shape, big headed man, big eared man, big mouthed man, man having prominent cheekbones, man having long waist, man having big hips, man with thick hair or white hair, man with bald on the head, etc. In color it is applied to darkish complexion, flushed tip of the nose, or flushed cheek bones. Through inquiring into the clinical cases on the points of Hyungsang medicine Yukmigiwhangwon is efficacious cure to lumbago, symptoms of tendons and bones, symptoms of eyes and ears, symptoms of respiratory organs, dermatopathia, headache, polydipsia, urinary disorders, etc.

• FLOWER RESEARCH JOURNAL
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• v.16 no.3
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• pp.200-204
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• 2008

A new spary chrysanthemum(Dendranthema grandiflorum) cultivar 'Serapina' was developed from a cross between 'Relanse' and 'Yen' by selection of seedlings and lines at the Flower Research Institute, Gyeongnam Agricultural Research and Extention Services(ARES) from 2002 to 2006. Its characteristics were investigated three times from 2004 to 2006 under condition of forcing culture in spring and retarding culture in autumn. The natural flowering time of 'Serapina' was October 23th, and year-round production was possible by day length treatment. Its capitulum was 2.8cm in diameter, and had 43.4 ray florets and 28.9 head per stem in autumn. Its ray floret was yellow with green central zone. To flower in the short day condition, for 'Serapina' was about 46 days in spring, and 'Serapina' showed the vase life of 21.7 days in autumn. This cultivar was registered for a commercialization in 2007.

• Proceedings of the Korea Water Resources Association Conference
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• 2019.05a
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• pp.267-267
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• 2019

Driftwood is one of serious problems in a river environment. In several countries, such as Indonesia, Japan, and Italy, the driftwood frequently appears in a river basin, and it can alter the channel bed, flow configuration by wood deposition and jam formation. Therefore, the studies related to driftwood have been actively conducted by many researchers to understand the mechanism of driftwood dynamics. In particular, wood motion by collision is one of the difficult issues in the numerical simulation because the calculation for wood collision requires significantly expensive calculation time due to small time step. Thus, this study conducted the numerical simulation in consideration of the wood motion by water flow and wood collision to understand the wood dynamics in terms of computation. We used the 2D (two-dimensional) depth-averaged velocity model, Nays2DH, which is a Eulerian model to calculate the water flow on the generalized coordinate. A Lagrangian type driftwood model, which expresses the driftwood as connected sphere shape particles, was employed to Nays2DH. In addition, the present study considered root wad effect by using larger diameter for a particle at a head of driftwood. An anisotropic bed friction was considered for the sliding motion dependent on stemwise, streamwise and motion directions. We particularly considered changeable draft at each particle and projection area by an angle between stemwise and flow directions to precisely reproduce the wood motions. The simulation results were compared with experimental results to verify the model. As a result, the simulation results showed good agreement with experimental results. Through this study, it would be expected that this model is a useful tool to predict the driftwood effect in the river flow.

• The Korean Journal of Malacology
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• v.22 no.1 s.35
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• pp.39-49
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• 2006

Ultrastructure of germ cell differentiation during supermatogenesis and the reproductive cycle in male Patinopecten yessoensis was studied by histological and cytological observations. The gonadosomatic index (GSI) in males rapidly increased and reached a maximum in April when seawater temperature gradually increased. Then the GSI gradually decreased from May through July when spawning occurred. Accordingly, monthly changes in the GSI in males coincided with testicular maturation and spawning periods. The sperm morphology of P. yessoensis belongs to the primitive type and showed general characteristics of external fertilization species. The head of the spermatozoon is approximately $3.50{\mu}m$ in length: the sperm nucleus and acrosome are approximately $2.90{\mu}m\;and\;0.60{\mu}m$ in length, respectively. The nuclear type of the spermatozoon is vase in shape, and the acrosome is cone type. The axoneme of the tail flagellum consists of nine pairs of microtubules at the periphery and a pair of central microtubules in the center The satellite body (which is formed by the centriole) and four mitochondria appear in the middle piece of the spermatozoon. The spawning period was from April through July and the main spawning occurred from May to June when seawater temperatures gradually increased. The reproductive cycle of this species can be classified into five successive stages; early active stage (September to November), late active stage (October to March), ripe stage (February to August), spawning stage (April to July), and spent/inactive stage (July to November).

• Journal of the Korean Society of Radiology
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• v.15 no.2
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• pp.181-189
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• 2021

This study is a prior research to manufacture a thermoplastic mask, which is a fixture used in radiation therapy, by 3D printing. It proceeded to analyze the filament material that can replace the thermoplastic. Among the commercially available filament materials, a material having similar characteristics to that of a thermoplastic mask was selected and the radiation dose was compared and analyzed. The experiment used Monte Carlo simulation. The shape in which the mask fixed the head was simulated for the ICRU sphere. The photon fluence was calculated at the skin Hp (0.07), the lens Hp (3), and the whole body Hp (10) by applying a thermoplastic plastic material and a filament material. As a result, when looking at the relative dose based on the thermoplastic plastic material, the difference was approximated within 4%. The material showing the most similar dose was PA-nylon. In selecting an appropriate filament material, it should be selected by comprehensively considering various conditions such as economical efficiency and radiation effects. It is thought that the results of this study can be used as basic data.

• Journal of radiological science and technology
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• v.42 no.1
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• pp.39-46
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• 2019

This study conducted a comparative analysis of differences between cartesian trajectory in a linear rectangular coordinate system and MultiVane trajectory in a nonlinear rectangular coordinate system axial T1 and axial T2 images using an American College of Radiology(ACR) phantom. The phantom was placed at the center of the head coil and the top-to-bottom and left-to-right levels were adjusted by using a level. The experiment was performed according to the Phantom Test Guidance provided by the ACR, and sagittal localizer images were obtained. As shown in Figure 2, slices # 1 and # 11 were scanned after placing them at the center of a $45^{\circ}$ wedge shape, and a total of 11 slices were obtained. According to the evaluation results, the image intensity uniformity(IIU) was 93.34% for the cartesian trajectory, and 93.19% for the MultiVane trajectory, both of which fall under the normal range in the axial T1 image. The IIU for the cartesian trajectory was 0.15% higher than that for the MultiVane trajectory. In axial T2, the IIU was 96.44% for the cartesian trajectory, and 95.97% for the MultiVane trajectory, which fall under the normal range. The IIU for the cartesian trajectory was by 0.47% higher than that for the MultiVane trajectory. As a result, the cartesian technique was superior to the MultiVane technique in terms of the high-contrast spatial resolution, image intensity uniformity, and low-contrast object detectability.