• Bulletin of the Korean Chemical Society
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• v.26 no.1
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• pp.151-156
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• 2005

The conformational dynamics of heme pocket, a small vacant site near the binding site of heme proteins -myoglobin (Mb) and hemoglobin (Hb), was investigated after photolysis of carbon monoxide from MbCO and HbCO in D$_2$O solution at 283 K by probing time-resolved vibrational spectra of photolyzed CO. Two absorption bands, arising from CO in the heme pocket, evolve nonexponentially in time. The band at higher energy side blue shifts and broadens with time and the one at lower energy side narrows significantly with a negligible shift. These spectral evolutions are induced by protein conformational changes following photolysis that modify structure and electric field of heme pocket, and ligand dynamics in it. The conformational changes affecting the spectrum of photolyzed CO in heme pocket likely modulates ligand-binding activity.

• Journal of Chest Surgery
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• v.47 no.4
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• pp.350-357
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• 2014

Background: Conservation of blood during cardiac surgery is important because of the shortage of donor blood, risks associated with transfusion, and the costs of allogeneic blood products. This retrospective study explored the feasibility of off-pump coronary artery bypass (OPCAB) without transfusion. Methods: One hundred and two consecutive patients underwent OPCAB from January 2007 to June 2012 at Hallym University Sacred Heart Hospital. Excluding 10 chronic renal failures patients, 102 patients were enrolled. Their characteristics, clinical data, and laboratory data were analyzed. We investigated the success rate of OPCAB without transfusion according to preoperative hemoglobin (Hb), and the cutoff point of the Hb level and the risk factors for transfusion. We implemented multidisciplinary blood-saving protocols. Results: The overall operative mortality and the success rate of OPCAB without transfusion were 2.9% (3/102) and 73.5% (75/102). The success rates in patients with Hb<11, 11 70 years, diagnosis of acute myocardial infarction, preoperative Hb and creatinine levels, and operation time. The events precipitating the need for transfusion were low Hb level in 9 patients and hypotension or excessive bleeding in 18 patients. Conclusion: The preoperative Hb level of >11 facilitates OPCAB without transfusion. These results suggest that transfusion-free OPCAB can be performed by modifying the risk factors and correctable causes of transfusion and improving various blood salvage methods.

• Journal of Nutrition and Health
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• v.22 no.3
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• pp.167-174
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• 1989

The purpose of this study was to determine if vitamin B-2 and vitamin B-6 deficiency affects hematologic profile in vivo. Rats were fed a vitamin B-2 deficient (-B2) diet or a vitamin B-6 deficient (- B6) diet or a combined vitamin B-2 and vitamin B-6 deficient (-B2-B6) diet or a control diet for 6 weeks. Hematocrit (Ht), hemoglobin (Hb), plasma iron (PI), transferrin saturation (TS) and liver iron concentration (LI) were compared. In -B2 rats vs. control rats, PI, TS and LI were significantly lower but Ht and Hb were not. In - B6 rats vs. control, Ht and Hb were significanty lower but LI was higher. The -B2-B6 rats had intermediate effects in Hb, PI, TS and LI and a decrease in Ht. The repletion with a contro diet for 2 weeks resulted in significant improvements in hematologic indices and LI in both - B2 rats and - B6 rats. This study suggests that the intakes of vitamin B-2 and vitamin B-6 are important for preventive and therap\ulcornereutic approaches to improve the hematologic status in nutritionally anemic groups.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.7
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• pp.3187-3190
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• 2012

This study evaluated the relationship between pretreatment hemoglobin (Hb) and prognostic factors in Thai patients with endometrial cancer. Medical records of 228 patients who had undergone surgery between January 2005 and December 2007 were retrospectively reviewed. Associations between clinicopathological variables and pretreatment Hb levels were described using Pearson's chi square test or two-tailed Fisher's exact test. Survival analysis was performed with Kaplan-Meier estimates. Univariate and Cox-regression models were used to evaluate the prognostic impact of various factors, including Hb levels, in term of disease-free survival. The median duration of follow-up was 38.2 months. Eighty-nine patients (39%) had a preoperative Hb level of <12 g/dL, these having significantly higher rates of non-endometrioid histology, advanced FIGO stage, lymphovascular space invasion, cervical involvement, adnexal involvement, positive peritoneal cytology, and lymph node involvement than patients with Hb ${\geq}12$ g/dL. The 5-year disease-free and overall survival were significantly lower in patients with pretreatment Hb levels <12 g/dL compared with those with Hb ${\geq}12$ g/dL (79.3% vs. 89.2%, p=0.044 and 87.6% vs. 99.3%, p<0.001, respectively). In the multivariate analysis only histology, myometrial invasion, and lymphovascular invasion proved to be independent prognostic factors, whereas tumor grading, stage, cervical involvement, adnexal involvement, positive peritoneal cytology, lymph node involvement, and low Hb were not. In conclusion, presence of anemia before treatment may reflect poor prognostic factors in patients with endometrial cancer and low pretreatment hemoglobin level may have a prognostic impact on clinical outcome.

• Bulletin of the Korean Chemical Society
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• v.31 no.7
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• pp.2103-2106
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• 2010

• Journal of Home Health Care Nursing
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• v.22 no.1
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• pp.14-21
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• 2015

Purpose: The purpose of this study was to identify relationships between HbA1c (Glycosylated Hemoglobin), WHR (Waist hip ratio), WTR (Waist thigh ratio) and compliance in elderly diabetes mellitus patients aged 65 years or over. Method: We conducted a survey and measured HbA1c, WHR, WTR in a total of 180 elderly patients with diabetes from 5 May 2014 to 30 May 2014. The data were analyzed by t-test, ANOVA, and Pearson's Correlation Coefficient using the SPSS program. Results: There were no significant differences in diabetes-related characteristics for HbA1c, WHR and WTR. However, patients with a family history had low compliance scores (p=.004). Furthermore, patients who visited the hospital regularly had higher compliance scores than patients who visited hospital when they were sick (p<.001). Patients with diabetic complications had low treatment compliance scores (p=.001). In addition, WHR and WTR (r=0.47, p<.001). and WHR and compliance (r=0.15, p=.045) showed positive correlation. Conclusion: For elderly diabetes mellitus patients, diabetes-related characteristics and compliance were highly related, so it is necessary to improve compliance for managing diabetes mellitus.

• Applied Biological Chemistry
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• v.44 no.3
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• pp.143-147
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• 2001

An osmometrical method for determination of the degree of hydrolysis (DH) of hemoglobin was comparatively examined through TNBS (trinitrobenzene) sulfonic acid) method using two experimental variables $({\alpha}\;and\;{\beta})$, which were chosen based on correlation curves between hydrolysis equivalents (h) and $leucine-NH_2$ equivalents. DH values measured through osmometry and TNBS method highly correlated with $R^2$ values of $0.974{\sim}0.991$, irrespective of the reaction pHs and types of enzyme used. $DH_{osm}/DH_{TNBS}$ was >$1.438{\sim}1.656$ depending on the hydrolysis pH $7.5{\sim}10.0)$. Correlation euqations were well fit for measuring DH of Hb hydrolysate at different pH conditions. $DH_{osm}/DH_{TNBS}$ for co-treatment system using Esperase and Flavourzyme was 1.658, in good agreement with that of 1.656 for the single enzyme (Esperase) system. Thus, the osmometrical method was suggested to be a convenient, reliable, and rapid method for determination of DH of hemoglobin hydrolysates.

• The Korean Journal of Physiology and Pharmacology
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• v.2 no.2
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• pp.193-199
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• 1998

To elucidate the mechanism involved in the cerebral vascular spasm following subarachnoid hemorrhage (SAH), the effects of the cerebrospinal fluid (CSF) obtained from the SAH patients on the resting tension and its influence on the contractile responses to various vasoactive agents and to hypoxia were investigated in isolated porcine cerebral arteries. All the CSFs containing hemoglobin (Hb) produced contraction and some Hb-free CSFs also elicited contraction. When the Hb-free CSF was separated by microfilter, the filtrate of ＜30,000 MW did not produce contraction, while the fraction above 30,000 MW elicited more marked contractile responses than the unfractionated CSF. The CSF contraction was significantly attenuated in the presence of indomethacin or nimodipine, whereas the contractions induced by KCl, prostaglandin $F_{2{\alpha}}$ ($PGF_{2{\alpha}}$), or endothelin-1 (ET-1) were not affected by the CSF pretreatment. However, the contractile responses induced by 5-hydroxytryptamine (5-HT) and phenylephrine (PE) were markedly potentiated by the pretreatment. Hypoxia-induced vasoconstriction was significantly potentiated by the pretreatment with either unfractionated CSF or the CSF fraction of above 30,000 MW. These results suggest that unknown vasocontractile substance(s) exists in the Hb-free CSF and that the substance, with its MW above 30,000, is activated by hypoxia and acts synergistically with 5-HT and PE, and that extracellular calcium influx and cyclooxygenase are also involved in the cerebral vasoconstrictory effect of Hb-free CSF.

• The Journal of Korean Medicine
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• v.32 no.1
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• pp.130-140
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• 2011

Objectives: This study was performed in order to investigate the anti-hemolytic effect of Chunghyuljihwang-tang(CHJHT, Qingxuedihuang-tang) on phenylhydrazine-induced hemolytic anemia in Rats. Materials and Methods: Hemolytic anemia model of rats were induced by phenylhydrazine(PHZ, 40mg/kg/day, i.p.) for 3days. Male Sprague-Dawley rats were divided into three groups for their treatment. Normal group was injected normal saline for 3 days with distilled water(d.w.) orally, control group was injected PHZ for 3 days with d.w. and CHJHT group was injected PHZ for 3 days with administration of CHJHT for 10 days. RBC(Red blood cell), Hb(hemoglobin), mean corpuscular volume, mean corpuscular hemoglobin, mean corpuscular hemoglobin concentration, reticulocyte were determined on day 0, 4, 7, 10. Body weight was investigated on day 0, 2, 4, 6, 8, 10 and the weight of spleen was measured on day 10. Results: For CHJHT group, the levels of RBC and Hb were significantly higher than control group on day 4, and the concentration of hemoglobin on day 7 was increased more than control group. Moreover, reticulocyte was significantly decreased on day 10 more than control group. These results could be concluded that CHJHT suppressed the hemolytic anemia by PHZ and improved hematological values of anemia earlier.

• Journal of Digital Convergence
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• v.17 no.3
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• pp.323-330
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• 2019

The purpose of this study was to investigate the effect of body mass index(BMI) and waist circumference(WC) on hemoglobin(Hb) in Korean Adult. This study analyzed the data of 2015, The Sixth KNHANES. Data were analyzed using descriptive statistics, t-test, ANOVA and Scheffe's test, Pearson correlation coefficient and multivariate logistic regression analysis(SPSS 25.0). The major findings, The subject's Hb In BMI, obese was the highest(F=97.862, p<0.001). The Hb In WC, $WC{\geq}90cm$ was higher for male(t=4.909, p<0.001), and $WC{\geq}85cm$ was higher for female(t=5.088, p<0.001). Hb has a significant positive correlated with BMI(r=0.188, p<0.001), WC(r=0.298, p<0.001). In male, Hyperhemoglobinemia was 2.30 times(p<0.001) higher in obese and 1.81 times(p<0.001) higher in $WC{\geq}90cm$. In female, Hyperhemoglobinemia was 1.99 times(p=0.002) higher in overweight, 5.66 times(p<0.001) higher in obese, and 4.27 times(p<0.001) higher in $WC{\geq}85cm$. In conclusion, adult obesity prevention and management programs should include exercises, diets, and education, and exercise interventions specifically for eliminating abdominal obesity.