• The Korean Journal of Malacology
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• v.24 no.3
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• pp.175-188
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• 2008

This study was performed to describe the influence of water temperature and salinity on the filtration rate of the hard clam, Meretrix petechialis. The filtration rates of hard clams showed significant differences depending on both water temperature and age group (two-way ANOVA, p < 0.001). The filtration rate of all the hard clams, aged from 1 to 4 years, was generally reduced in low temperature range ( $5-15^{\circ}C$). As the water temperature increased, the filtration rate increased exponentially. The filtration rate was relatively high in $20-30^{\circ}C$ water temperature range, but rapidly decreased again at around $35^{\circ}C$. Variations in the data for filtration rates of hard clams, relative to age and changing water temperature, were used to determine the temperature coefficient $Q_{10}$ in each water temperature range. In $5-15^{\circ}C$ temperature range, every age group showed the highest $Q_{10}$ figure. It was found that on the whole, the higher the water temperature was, the lower the number of $Q_{10}$ was. Regardless of age, the number of $Q_{10}$ was higher in the lower water temperature range. In $25-35^{\circ}C$ temperature range, the number of $Q_{10}$ was less than 1.00 in all age groups, implying that this is the range in which the filtration rate decreased. Variations in the filtration rate also showed very clear differences (two-way ANOVA, p < 0.001) according to changing salinity and age. All the age groups, from 1 to 4 year olds, showed low filtration rates at both low salinity (10-15 psu) and high salinity (40 psu). The highest filtration rate was recorded at 30 psu, and relatively high filtration rates were found around 30 psu. However, the Student-Newman-Keuls post hoc multiple comparison test found that the hard clams in the 1-year group showed high filtration rates in a relatively narrower salinity range compared to those in the 2, 3, and 4 year groups. In other words, hard clams in the 2, 3, and 4 year groups showed high filtration rates at higher levels of salinity.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.1
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• pp.16-21
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• 1999

Taurine content was determined by HPLC equipped with RF detector in commercial milks, meats, edible viscera, ham, sausage, fishes and shellfishes, mollusca and crustacea. Taurine contained 1.06~1.57mg/100g in milks, 37.9~169.8mg/100g in lean meats, 11.3~192.4mg/100g in viscera, 45.0~109.1 mg/100g in ham, 11.7~26.0mg/100g in sausage, 31.6~431.9mg/100g in fishes, 125.9~1078.8mg/100g in shellfishes, 340.2~ 1305.6mg/100g in mollusca and 79.3~316.5mg/100g in crustacea. Webfoot octopus had the highest content of 1,306mg/100g, followed by small ark shell(1,079mg/100g), little neck clam (868mg/100g), hard shelled mussel(852mg/100g) and beka squid(734mg/100g).

• The Korean Journal of Malacology
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• v.25 no.2
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• pp.161-171
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• 2009

The present study was performed to describe the influence of water temperature and salinity on filtration rates of the venus clam, Gomphina veneriformis, a suspension-feeding (filter-feeding) bivalve species. The calmswere collected from the eastern coastal area of Sokcho, Gangneung and Jumunjin at Kangwon-do, Korea, during December 2006 and May 2007. Isochrysis galbana (KMCC H-002) cells as food organisms were indoor-cultured by f/2 medium, and were used to measure the filtration rate of clam. Filtration rates of clam were measured by indirect method. Cell concentration of food organisms were determined by direct counting cells used the hemacytometer under the light microscope. The filtration rates of clams by water temperature sharply increased with temperatures up to $15^{\circ}C$ as optimum temperature and above this temperature, the filtration rates decreased exponentially. Venus clams showed very low filtration rates at low salinity (10-15 psu) and maximum values at high salinity (30-35 psu). Regardless of water temperature and salt change, 2-year class clams showed high filtration rates, but low in 4-year-class. Polynomial regression curves with water temperature were shifted to the left in low temperature region. Thermal coefficient $Q_{10}$ values showed much higher values at low temperature range than at high temperature range, too. These results indicate that the venus clam is more sensitive in cold water. Polynomial regression curves with salinity were shifted to the right in high saline region. According to this study, the venus clam Gomphina veneriformis, subtidal filter-feeding bivalve, was the stenothermal organism, inhabited mainly in low temperature and the stenohaline, in high saline waters.

• The Korean Journal of Malacology
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• v.27 no.3
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• pp.167-173
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• 2011

This study was performed to describe the effect of water temperature, salinity and density on the eggs development of the hard clam, Meretrix petechialis. Eggs of Meretrix petechialis were turned out to be demersal isolated eggs of $82.3-86.1{\mu}m$ in an average diameter after spawning. The hatching rate of D-shaped larvae by elapsed time after spawning was the highest in fertilization immediately after spawning and distinguished decrease from 1 hour of spawning. The optimum water temperature for development of D-shaped larvae from fertilization was ranged between $25^{\circ}C$ to $27^{\circ}C$. However, D-shaped larvae was not developed at $33^{\circ}C$ of water temperature. The required time from fertilization to D-shaped larvae were 37.3 hours in $20^{\circ}C$, 20.8 hours in $25^{\circ}C$, and 15.3 hours in $30^{\circ}C$. Biological minimum temperature for the egg development was estimated to be $12.4^{\circ}C$ in average. The range of salinity for the development of eggs were 20.0-37.5 psu, optimum range was estimated to be 27.5-32.5 psu.

• The Korean Journal of Malacology
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• v.25 no.2
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• pp.113-119
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• 2009

Artificial induction of maturation by heating of the hard clam, Meretrix petechiails (Lamarck) broodstock was investigated from 25 May to 30 June in 2008. We experimented maturation by keeping the breeding water temperature at 20, 25 and $30^{\circ}C$ and as the natural water temperature and found the following results. In case of the female, maturation was the quickest in the experiment group of $30^{\circ}C$ and the maturation tended to be slower as the water temperature was lower. The similar trend was displayed among the male. At experiment completion, maturation induction rate was found to be the highest at 46.0% in the experiment group of $30^{\circ}C$ followed by 35.0% in the experiment group of $15^{\circ}C$, 18.0% in the experiment group of natural water temperature and 12.0% in the experiment group of $20^{\circ}C$. During the period of the experiment, the survival rate was of 90% or more in all groups.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.11 no.4
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• pp.152-157
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• 2006

Samples of Meretrix lusoria were collected monthly from the tidal flat of Simpo, Puan-gun, Chollabuk-do, west coast of Korea from April 2004 to March 2005. Age of M. lusoria was determined from the rings on the shell. The relationship between shell length and ring radius in each ring group was expressed as a regression line. Therefore, there is a correspondence in each ring formation. Based on the monthly variations in the marginal index (MI') of the shell, it is assumed that the ring of this species was formed once a year during the period of February to April. The relationship between shell length (SL) and shell height (SH; mm) was highly correlated with shell height as the following equation: SH = 0.8103 SL + 0.5145 $(r^2=0.991)$. The shell length (SL) - shell width (SW) relation was also expressed by the following equation: SW = 0.4897 SL + 0.0315 $(r^2=0.976)$. Shell length (SL; mm) and total weight (TW; g) was expressed by the following equation: $TW=2.9195\times10^{-4}\;SL^{2.9547}\;(R^2=0.991)$. Shell length (SL) and shell height (SH; mm) was highly correlated with shell height as the following equation: $SH=0.8103\;SL+0.5145\;(R^2=0.991)$ The shell length (SL) - shell width (SW) relation was also expressed by the following equation: $SW=0.4897\;SL+0.0315\;(R^2=0.976)$. Growth curves for shell length and total weight fitted to the von Bertalanffy's growth curve were expressed respectively as: $SL_t=104.9[l-e^{-0.2235(t+0.7677)}],\;TW_t=280.8[l-e^{-0.2235(t+0.7677)}]^{2.9547}$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.17 no.1
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• pp.61-67
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• 1984

The life history of a trematod fluck, Himasthla kusasigi was studied on the morphological characters of metacercariae, its infection rate in the clam and the contamination experiment to the herring gull, Larus crassiostris. The size of the metacercariae was $210{\sim}230{\times}220{\mu}m$, excysted metacercariae was $420{\times}160{\mu}m$ and it had 31 collar spines. The ventral sucker($112{\times}100{\mu}m$) was located just below the central part of the body. Esophagus was narrow and long, the intestine was branched in the upper part of the ventral sucker and extended to the hind part of the body. Excretory bladder without the infected part was located in the hind part of the body. The branched excretory tube was filled with the small granules and extended to the pharynx area. The infection rate of the metacercariae varied from place to place. In Naecho do and Puan the rates were $98.4\%$ and $95.9\%$ respectively. The infected number of Himasthla kusasigi per clam was positively proportional to the size of the clam. The adult fluckes developed from the metacercariae were obtained from the experimented herring gull. The metacercariae emerge from their cysts in the stomach of the herring gull, then they migrate it the intestine and 25 days after, most of them were found in the intestine. The size of the adult was $4.8{\sim}7.5{\times}0.22{\sim}0.24mm$, oral sucker; $68{\sim}120{\times}70{\sim}120{\mu}m$, pharynx: $80{\times}50{\mu}m$, ventral sucker: $630{\sim}680{\times}610{\sim}680{\mu}m$. The head collar $48{\sim}75{\times}10{\mu}m$ had 31 collar spines. The isolated trematod fluck from the samples was classified as a Himasthla kusasigi by the morphological characteristics of the adult flock and its metacercariae. We oberved that the second intermediate host of Himasthla kusasigi was hard clam, Meretrix lusoria, while its final host was herring gull, Larus crassiostris and Tringa ochropus.

• The Korean Journal of Malacology
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• v.27 no.4
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• pp.353-358
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• 2011

This study is the result of examining the growth and survival rate of larva and spat when supplied with Enriched live food by adding $B_{12}$, chitosan, PSB, and polysaccharides to microalgae (Chaetoceros gracilis, Isochrysis galbana) to raise the survival rate of larva and spat during artificial clam seed production. Microalge (Chaetoceros gracilis, Isochrysis galbana) was strengthened for nutrition with $B_{12}$ 0.1 ppm, chitosan 0.2 ppm, PSB 3 ppm and polysaccharides 1 ppm and was daily supplied for $3{\times}10^3-15{\times}10^3Cells/mL$ and the growth and survival rates were measured. As the result of experiment, the growth of larva did not show much difference with PSB test section $199{\pm}0.59{\mu}m$, $B_{12}$ test section $198{\pm}0.64{\mu}m$, and chitosan $197{\pm}0.52{\mu}m$, survival rate was highest at PSB test section with 99.3%, followed by $B_{12}$ test section 95.9%, and chitosan 94.5%. Growth of early spat was the highest for PSB test section at $2.74{\pm}0.58mm$, followed by polysaccharides $2.67{\pm}0.55mm$, $B_{12}$ $2.54{\pm}0.48mm$, and chitosan $2.49{\pm}0.51mm$, and the survival rate was the highest for PSB test group at 32.1%, followed by $B_{12}$ test section 31.6%, chitosan 28.5%, and polysacharrides 21.4%. From such results, PSB is found to be very effective with low-quality improvement when breeding early spat of clams, and especially for floor-type early spat breeding, it had the effect of suppressing protozoan and germs so that detailed studies from various perspectives should be conducted with various chemicals in the future.

• The Korean Journal of Malacology
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• v.27 no.2
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• pp.115-119
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• 2011

To establish technical development for artificial seed production, growth and survival for early young spats of the hard clam, Meretrix petechialis, were investigated by breeding methods. Adult clams were collected at Hasa-ri, Baeksu-eup, Yeonggwang-gun, Jeollanam-do on July 13, 2010, and then transported to the indoor aquarium at the laboratory. Eggs which were taken from mother clams, were inseminated, and after they were fertilized in the aquarium, 60 million bottom-clinging spats ($198{\pm}12{\mu}m$ in shell length) were produced and bred. The breeding experiments were carried out from July 16 to October 4, 2010 for 80 days. The methods of sand box, sand bottom circulation filter, inclosing net, floor were used for the breeding experiments, and the experimental condition of sea water temperature for larvae were at 25, 28, 31, $34^{\circ}C$. Four marine cultured food organisms were used for this study as follows: Isochrysis galbana, Chaetoceros gracilis, Phaeodactylum tricornutum, Tetraselmis tetrathele. According to the experimental conditions, experimental groups of the spats in the early stage were investigated the growth rate and the survival. As the result, the method of the inclosing net section was the fastest (grew up to $2.64{\pm}0.59{\mu}m$ in shell length), followed by sandbox ($2.59{\pm}0.64{\mu}m$, bottom circulating filter ($2.56{\pm}0.52{\mu}m$), and floor ($2.52{\pm}0.56{\mu}m$). The survival was the highest in the experimental condition of sandbox (35.9%), followed by floor (34.6%), bottom circulating filter (29.5%), and inclosing net (9.3%). Eexperimental condition of water temperature of $34^{\circ}C$ showed the fastest growth rate (grew up to $2.70{\pm}0.76{\mu}m$ in shell length), and showed the latest growth rate (grew up to $2.45{\pm}0.41{\mu}m$ in shell length) at $25^{\circ}C$. The survival (%) was the highest under the water temperature conditions at $31^{\circ}C$, and showed the lowest (14.2%) at $34.^{\circ}C$. The growth rate of the experimental group fed the mixture live food was the fastest with shell length $2.52{\pm}0.66{\mu}m$, and that of experimental group fed P. tricornutum showed the latest (grew up to $2.29{\pm}0.43{\mu}m$ in shell length). The survival was the highest (36.9%) under the experiment condition fed mixture live food and experimental group fed T. tetrathele showed the lowest rate (16.2%).

• Korean Journal of Food Science and Technology
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• v.8 no.4
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• pp.219-224
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• 1976

The appearance of organochlorine pesticide residues in shellfish, seaweed, mud and water samples collected from south coast of Korean peninsula in September 1974 was surveyed to obtain the following results. 1) The residues in shellfish samples were found to be in the orders of Choongmoo > Yeosoo > Busan > Mokpo area, top-shell >short-necked clam > mussel > hard clam > red-shell, aldrin > ${\alpha}-BHC$ > heptachlor> ${\gamma}-BHC$ > dieldrin > heptachlor epoxide > endrin and in the range of $0-49\;{\mu}g/kg$ edible fresh muscle. 2) The residues in seaweed samples from Wando area were found to be in the orders of tangle > green laver > laver, ${\alpha}-BHC$ > ${\gamma}-BHC$ > aldrin > dieldrin > heptachlor > heptachlor epoxide > endrin and in the range of $0-60\;{\mu}g/kg$ dry matter. 3) The residues in water samples were found to be in the orders of Yeongsan River > Nakdong River > Seomjin River > Choongmoo shellfish farm, ${\alpha}-BHC$ > ${\gamma}-BHC$ > aldrin > heptachlor > dieldrin > heptachlor epoxide > endrin and in the range of $0-180\;{\mu}g/kl$ while those in mud samples were in the order of ${\alpha}-BHC$ > ${\gamma}-BHC$> aldrin > dieldrin > heptachlor > heptachlor epoxide > endrin and in the range of $0.7-18\;{\mu}g/kg$ dry matter. It should be noted that BHC contents of water samples from Yeongsan and Nakdong rivers were far above the recommended environmental levels in USA.