• Title/Summary/Keyword: Hanwoo (Korean cattle)

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Reproductive performance of Korean native cattle (Hanwoo) focusing on calving interval and parity (분만간격과 산차를 중심으로 한국 재래종인 한우의 번식능력 분석)

  • Cho, Jaesung;Do, Changhee;Choi, Inchul
    • Journal of Embryo Transfer
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    • v.31 no.3
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    • pp.273-279
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    • 2016
  • The Korean native cattle, Hanwoo, is the most popular breed of beef cattle in Korea. However, the reproductive performance data are limited although reproduction is one of the most economically and biologically important in beef production. Therefore, this study was undertaken to investigate reproductive performance parameters including calving interval, parity for life time production. Data collected from 206,827 calvings were analyzed. There were no significant differences in calving interval and gestation days as parity increased from 2nd and 13rd parity cow, from spring to winter. However, we found a dramatic increase in calving interval after year 2000. About 1 month were increased per year ( y = 30.578x + 344.45 $R^2=0.9157$). Interestingly, we observed that parities for life time can be affected by birth weight. Calves with 23 kg at birth showed highest parities, $3.4{\pm}2.0$ times. In summary, this study provides valuable data on reproductive performance of Hanwoo and the data presented here can be used as a standard target for optimising and enhancing reproductive performance.

The characteristics of bovine satellite cells with highly scored genomic estimated breeding value

  • Jae Ho Han;Ji Suk Yu;Do Hyun Kim;Hyun Woo Choi
    • Journal of Animal Reproduction and Biotechnology
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    • v.38 no.3
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    • pp.177-187
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    • 2023
  • Background: The grading of Hanwoo (Korean native cattle) is based on four economic traits, and efforts have been continuously made to improve the genetic traits associated with these traits. There is a technology to predict the expected grade based on the 4 economic genetic SNP characteristics of Korean cattle calves using single nucleotide polymorphism (SNP) technology. Selection of highly proliferative, self-renewing, and differentiating satellite cells from cattle is a key technology in the cultured meat industry. Methods: We selected the Hanwoo with high and low-scored of genomic estimated breeding value (GEBV) by using the Hanwoo 50K SNP bead chip. We then isolated the bovine satellite cells from the chuck mass. We then conducted comparative analyses of cell proliferation, immunocytochemistry, qRT-PCR at short- and long-term culture. We also analyzed the differentiation capability at short term culture. Results: Our result showed that the proliferation was significantly high at High scored GEBV (Hs-GEBV) compared to Low scored GEBV (Ls-GEBV) at short- and long-term culture. The expression levels of Pax3 were significantly higher in Hs-GEBV bovine satellite cells at long-term culture. However, there were no significant differences in the expression levels of Pax7 between Hs- and Ls-GEBV bovine satellite cells at short- and long- term culture. The expression levels of MyoG and MyHC were significantly high at Ls-GEBV bovine satellite cells. Conclusions: Our results indicated that selection of bovine satellite cells by Hanwoo 50K SNP bead chip could be effective selection methods for massive producing of satellite cells.

Genetic assessment of BoLA-DRB3 polymorphisms by comparing Bangladesh, Ethiopian, and Korean cattle

  • Mandefro, Ayele;Sisay, Tesfaye;Edea, Zewdu;Uzzaman, Md. Rasel;Kim, Kwan-Suk;Dadi, Hailu
    • Journal of Animal Science and Technology
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    • v.63 no.2
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    • pp.248-261
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    • 2021
  • Attributable to their major function in pathogen recognition, the use of bovine leukocyte antigens (BoLA) as disease markers in immunological traits in cattle is well established. However, limited report exists on polymorphism of the BoLA gene in zebu cattle breeds by high resolution typing methods. Thus, we used a polymerase chain reaction sequence-based typing (PCR-SBT) method to sequence exon 2 of the BoLA class II DRB3 gene from 100 animals (Boran, n = 13; Sheko, n = 20; Fogera, n = 16; Horro, n = 19), Hanwoo cattle (n = 18) and Bangladesh Red Chittagong zebu (n = 14). Out of the 59 detected alleles, 43 were already deposited under the Immuno Polymorphism Database for major histocompatibility complex (IPD-MHC) while 16 were unique to this study. Assessment of the level of genetic variability at the population and sequence levels with genetic distance in the breeds considered in this study showed that Zebu breeds had a gene diversity score greater than 0.752, nucleotide diversity score greater than 0.152, and mean number of pairwise differences higher than 14, being very comparable to those investigated for other cattle breeds. Regarding neutrality tests analyzed, we investigated that all the breeds except Hanwoo had an excess number of alleles and could be expected from a recent population expansion or genetic hitchhiking. Howbeit, the observed heterozygosity was not significantly (p < 0.05) higher than the expected heterozygosity. The Hardy Weinberg equilibrium (HWE) analysis revealed non-significant excess of heterozygote animals, indicative of plausible over-dominant selection. The pairwise FST values suggested a low genetic variation among all the breeds (FST = 0.056; p < 0.05), besides the rooting from the evolutionary or domestication history of the cattle. No detached clade was observed in the evolutionary divergence study of the BoLA-DRB3 gene, inferred from the phylogenetic tree based on the maximum likelihood model. The investigation herein indicated the clear differences in BoLA-DRB3 gene variability between African and Asian cattle breeds.

National Genetic Evaluation (System) of Hanwoo (Korean Native Cattle)

  • Park, B.;Choi, T.;Kim, S.;Oh, S.H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.26 no.2
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    • pp.151-156
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    • 2013
  • Hanwoo (Also known as Korean native cattle; Bos taurus coreanae) have been used for transportation and farming for a long time in South Korea. It has been about 30 yrs since Hanwoo improvement began in earnest as beef cattle for meat yield. The purpose of this study was to determine the trend of improvement as well as to estimate genetic parameters of the traits being used for seedstock selection based on the data collected from the past. Hanwoo proven bulls in South Korea are currently selected through performance and progeny tests. National Hanwoo genetic evaluations are implemented with yearling weight (YW), carcass weight (CW), eye muscle area (EMA), backfat thickness (BF) and marbling score (MS). Yearling weights and MS are used for selecting young bulls, and EMA, BF, and MS are used for selecting proven bulls. One individual per testing room was used for performance tests, and five individuals per room for progeny tests. Individuals tested were not allowed to graze pasture, but there was enough space for them to move around in the testing room. Feeds including roughages and minerals were fed ad libitum, and concentrates were provided at the rate of about 1.8% of individual weight. Overall means of the traits were $352.8{\pm}38.56$ kg, $335.09{\pm}44.61$ kg, $77.85{\pm}8.838\;cm^2$, $8.6{\pm}3.7$ mm and $3.293{\pm}1.648$ for YW, CW, EMA, BF and MS. Heritabilities estimated in this study were 0.30, 0.30, 0.42, 0.50 and 0.63 in YW, CW, EMA, BF and MS, respectively, which are similar to results from previous research. Yearling weight was 315.54 kg in 1998, and had increased to 355.06 kg in 2011, resulting in about 40 kg of improvement over 13 yrs. YW and CW have improved remarkably over the past 15 yrs. Breeding values between 1996 and 2000 decreased or did not change much, but have moved in a desirable direction since 2001. These improvements correspond with the substantial increase in use of animal models since the late 1990s in Korea. Hanwoo testing programs have practically contributed to the improvement in aspects of quality and quantity. In sum, the current selection system is good enough to accommodate circumstances where fewer sires are used on many more cows. Although progeny tests take longer and cost more, they seem to be appropriate under the circumstances of the domestic market with its higher requirement for better meat quality. Consequently, accumulative data collection, genetic evaluation model development, revision of selection indices, as well as cooperation among farms, associations, National Agricultural Cooperative Federation, universities, research institutes, and government agencies must be applied to the Hanwoo selection program. All these efforts will assist the domestic market to secure a competitive position against imported beef under Free Trade Agreement trade system and will provide farmers with higher profits as well as the public with a higher quality of beef.

Heat waves impair cytoplasmic maturation of oocytes and preimplantation development in Korean native cattle (Hanwoo)

  • Sa, Soo Jin;Jeong, Jiyeon;Cho, Jaesung;Lee, Seung-Hwan;Choi, Inchul
    • Korean Journal of Agricultural Science
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    • v.45 no.3
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    • pp.493-498
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    • 2018
  • There has been widespread warming and a general increase in summer temperatures over the Korean peninsula ($0.5^{\circ}C$/10 years from 2001 to 2010). South Korea is transforming into a subtropical region, and the productivity of livestock is affected by the climatic changes. In this study, we investigated whether the summer heat waves affect the developmental competency of Korean native cattle (Hanwoo), a taurine type of cattle with a small portion of indicine varieties. We collected oocytes during the summer (heat stress, HS) and autumn (non-HS condition) and examined the developmental competencies including in vitro maturation and preimplantation embryo development. No significant differences were observed between the HS and non-HS oocytes in nuclear maturation (extrusion of the polar body); however, the cleavage and blastocyst rates were significantly lower in the HS group than those in the non-HS group. The lower developmental competence of the HS oocytes compared to the non-HS is, in part, due to insufficient cytoplasmic maturation because of a higher production of Reactive oxygen species (ROS) levels as well as peri/cortical distributed mitochondria in the HS oocytes after in vitro maturation. Next, we examined the ROS and mitochondria distribution and found a significant increase in the levels of ROS in the HS oocytes and a polarized distribution (pericortical cytoplasm) of mitochondria in the HS oocytes. In summary, impaired cytoplasmic maturation of oocytes from exposure to HS affects the preimplantation embryo development by dysfunction of mitochondria. To improve reproductive performance, embryo transfer using cryopreserved embryos/oocytes is recommended in the hot summer season of South Korea.

Localization of 5,105 Hanwoo (Korean Cattle) BAC Clones on Bovine Chromosomes by the Analysis of BAC End Sequences (BESs) Involving 21,024 Clones

  • Choi, Jae Min;Chae, Sung-Hwa;Kang, Se Won;Choi, Dong-Sik;Lee, Yong Seok;Park, Hong-Seog;Yeo, Jung-Sou;Choi, Inho
    • Asian-Australasian Journal of Animal Sciences
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    • v.20 no.11
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    • pp.1636-1650
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    • 2007
  • As an initial step toward a better understanding of the genome structure of Korean cattle (Hanwoo breed) and initiation of the framework for genomic research in this bovine, the bacterial artificial chromosome (BAC) end sequencing of 21,024 clones was recently completed. Among these clones, BAC End Sequences (BESs) of 20,158 clones with high quality sequences (Phred score ${\geq}20$, average BES equaled 620 bp and totaled 23,585,814 bp), after editing sequencing results by eliminating vector sequences, were used initially to compare sequence homology with the known bovine chromosomal DNA sequence by using BLASTN analysis. Blast analysis of the BESs against the NCBI Genome database for Bos taurus (Build 2.1) indicated that the BESs from 13,201 clones matched bovine contig sequences with significant blast hits (E<$e^{-40}$), including 7,075 single-end hits and 6,126 paired-end hits. Finally, a total of 5,105 clones of the Korean cattle BAC clones with paired-end hits, including 4,053 clones from the primary analysis and 1,052 clones from the secondary analysis, were mapped to the bovine chromosome with very high accuracy.

Mannheimiosis case in Hanwoo caused by heat stress (한우에서 고온스트레스에 의한 Mannheimiosis 발생 증례)

  • Lee, Jeong-Won;Shon, Ku-Rye;Jeong, Han-Sol;Ko, Won-Seok;Lim, Chae-Woong;Kim, Bum-Seok
    • Korean Journal of Veterinary Service
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    • v.38 no.3
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    • pp.195-198
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    • 2015
  • Mannheimia haemolytica is an opportunistic bacterium that is widely recognized among the bovine respiratory disease (BRD) complex in cattle. Five Hanwoo with a history of fever, anorexia and dyspnea were died within 2 days in a the middle of summer. Four cattle were pregnant. The cattle house were located in mountainous area but the window for air ventilation was open only one side. In addition, the fecal material for fermentation was located indoor. Air ceiling fan did not work. The indoor temperature was $40^{\circ}C$. After working on air fan, the indoor temperature was still $36^{\circ}C$. On necropsy, there was fibrinous pleuritis with a rich yellowish pleural fluids in the thorax. The cross-section of the lung showed lobar fibrinonecrotic pneumonia with expanded interlobular septa by edema and fibrin. Microscopically, parenchymal necrosis with dense layer of inflammatory cells were observed surrounding interlobular septum. Fibrin and inflammatory cells were filled in the alveoli. Bacteriological cultures of pulmonary tissue showed growth of M. haemolytica. This pneumonia case in Hanwoo suggests that environmental stressors such as high temperature, insufficient air ventilation, and pregnancy be the cause of mannheimoisis. Control of environmental stressor, such as temperature indoor is necessary to prevent BRD caused by M. haemolytica.

Effects of Applying Cattle Slurry and Mixed Sowing with Legumes on Productivity, Feed Values and Organic Stock Carrying Capacity of Winter Forage Crops in Gyeongbuk Regions (경북지역에서 액상우분뇨 시용과 콩과작물의 혼파가 동계사료작물의 생산성, 사료가치 및 단위면적당 유기가축 사육능력에 미치는 영향)

  • Hwangbo, Soon;Jo, IK-Hwan
    • Korean Journal of Organic Agriculture
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    • v.21 no.3
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    • pp.451-465
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    • 2013
  • This study was conducted to estimate Hanwoo carrying capacity when whole crop barley, rye, wheat and tritcale as winter forage crops was grown on different applying sources (chemical fertilizer, cattle or organic fertilizer) and mixed sowing combination with hairy vetch or forage pea during the period of 2011~2012. The experimental plots within whole crop barley or rye were consisted of 7 treatments, which were non-fertilizer, chemical fertilizer (P+K), chemical fertilizer (N+P+K), organic fertilizer, cattle slurry, cattle slurry with hairy vetch, and cattle slurry with forage pea. Each plot was triplicates and experimental treatments were allocated in the randomized complete block design. For whole crop barley, annual mean dry matter (DM) and total digestible nutrients (TDN) yields were the highest in N+P+K plots, but there were no significant differences among organic fertilizer, cattle slurry and mixed sowing with legumes. The TDN were the highest in mixed sowing plots of forage pea plus cattle slurry application. As 450 kg Hanwoo heifers were fed diets included 70% whole crop barley, organic fertilizer, cattle slurry application and mixed sowing plots of forage pea is capable of raising average 2.8 to 3.1 heads/ha a year. For whole crop rye, annual mean DM were the highest in N+P+K plots, but there were no significant differences among cattle slurry. Organic fertilizer application significantly increased TDN and relative feed value (RFV) in comparison with treatments of N+P+K fertilization as chemical fertilizers. In case of 450 kg Hanwoo heifers fed diets included 70% forage rye, it is estimated that cattle slurry application (mixed sowing with legumes) plots can rear average 2.8~ 3.2 heads/ha a year. For whole crop wheat, annual DM, crude protein, and TDN yields of application groups and mixed sowing treatment with legumes showed 6.90~7.44, 0.53~0.60 and 4.35~5.04 ton/ha, respectively. In case of 450 kg Hanwoo heifers fed diets included 70% forage rye, it is estimated that cattle slurry application (mixed sowing with legumes) plots can rear average 3.1~3.7 heads/ha a year. For Triticale, TDN yield was significantly (P<0.05) higher N+P+K plots, organic ferilizer, cattle slurry, cattle slurry with legumes than for no fertilizer and N+P+K plots. The Crude protein (CP) contents were the highest in mixed sowing plots of forage pea plus cattle slurry application. In case of 450 kg Hanwoo heifers fed diets included 70% forage triticale, it is estimated that cattle slurry application (mixed sowing with legumes) plots can rear average 3.4~3.7 heads/ha a year. It can be concluded that, on the basis of DM yield, not only mixed sowing with legumes by applying cattle slurry rather than single sowing of whole crop barley or whole crop rye enhanced production yield and feed values, but also it could be a substitute for imported grains as dietary protein sources in the case of feeding Hanwoo.

Analysis of genetic diversity and distances in Asian cattle breeds using microsatellite markers

  • Shi, Zheng;Lee, Ji-Hong;Lee, Yoon-Seok;Oh, Dong-Yeub;Yeo, Jung-Sou
    • Journal of the Korean Data and Information Science Society
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    • v.21 no.4
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    • pp.795-802
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    • 2010
  • This study defined the genetic diversity of five breeds of cattle in Asia by analyzing 6 microsatellite markers in 270 animals. Based on expected mean heterozygosity, the lowest genetic diversity was exhibited in Japanese black cattle (HE=0.5849), and the highest in Chinese yellow cattle (HE=0.8073). Average proportion of genetic variation due to interpopulation subdivision among these five cattle breeds varied between 11.7 and 12.5%. The genetic distances were roughly divided into three groups: Japanese black cattle, Holstein, and the three remaining breeds. This clustering agrees with the origin and geographical distributions of these five cattle breeds.

Linkage Disequilibrium and Effective Population Size in Hanwoo Korean Cattle

  • Lee, S.H.;Cho, Y.M.;Lim, D.;Kim, H.C.;Choi, B.H.;Park, H.S.;Kim, O.H.;Kim, S.;Kim, T.H.;Yoon, D.;Hong, S.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.12
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    • pp.1660-1665
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    • 2011
  • This study presents a linkage disequilibrium (LD) analysis and effective population size ($N_e$) for the entire Hanwoo Korean cattle genome, which is the first LD map and effective population size estimate ever calculated for this breed. A panel of 4,525 markers was used in the final LD analysis. The pairwise $r^2$ statistic of SNPs up to 50 Mb apart across the genome was estimated. A mean value of $r^2$ = 0.23 was observed in pairwise distances of <25 kb and dropped to 0.1 at 40 to 60 kb, which is similar to the average intermarker distance used in this study. The proportion of SNPs in useful LD ($r^2{\geq}0.25$) was 20% for the distance of 10 and 20 kb between SNPs. Analyses of past effective population size estimates based on direct estimates of recombination rates from SNP data demonstrated that a decline in effective population size to $N_e$ = 98.1 occurred up to three generations ago.