Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Localization of 5,105 Hanwoo (Korean Cattle) BAC Clones on Bovine Chromosomes by the Analysis of BAC End Sequences (BESs) Involving 21,024 Clones

  • Choi, Jae Min (Institute of Biotechnology and Department of Biotechnology,Yeungnam University) ;
  • Chae, Sung-Hwa (Korea Research Institute of Bioscience and Biotechnology) ;
  • Kang, Se Won (Department of Parasitology, College of Medicine and Frontier Inje Research for Science and Technology, Inje University) ;
  • Choi, Dong-Sik (School of Biotechnology, Yeungnam University) ;
  • Lee, Yong Seok (Department of Parasitology, College of Medicine and Frontier Inje Research for Science and Technology, Inje University) ;
  • Park, Hong-Seog (Korea Research Institute of Bioscience and Biotechnology) ;
  • Yeo, Jung-Sou (Institute of Biotechnology and Department of Biotechnology,Yeungnam University) ;
  • Choi, Inho (Institute of Biotechnology and Department of Biotechnology,Yeungnam University)
  • Received : 2006.11.19
  • Accepted : 2007.04.07
  • Published : 2007.11.01
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Abstract

As an initial step toward a better understanding of the genome structure of Korean cattle (Hanwoo breed) and initiation of the framework for genomic research in this bovine, the bacterial artificial chromosome (BAC) end sequencing of 21,024 clones was recently completed. Among these clones, BAC End Sequences (BESs) of 20,158 clones with high quality sequences (Phred score ${\geq}20$, average BES equaled 620 bp and totaled 23,585,814 bp), after editing sequencing results by eliminating vector sequences, were used initially to compare sequence homology with the known bovine chromosomal DNA sequence by using BLASTN analysis. Blast analysis of the BESs against the NCBI Genome database for Bos taurus (Build 2.1) indicated that the BESs from 13,201 clones matched bovine contig sequences with significant blast hits (E<$e^{-40}$), including 7,075 single-end hits and 6,126 paired-end hits. Finally, a total of 5,105 clones of the Korean cattle BAC clones with paired-end hits, including 4,053 clones from the primary analysis and 1,052 clones from the secondary analysis, were mapped to the bovine chromosome with very high accuracy.

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References

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