• Journal of Embryo Transfer
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• v.15 no.1
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• pp.39-46
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• 2000

This study was conducted to investigate the effect of hormones, protein sources and anti-oxidants on in vitro maturation (IVM) and in vitro fertilization(IVF) of bovine follicular oocytes. The rates of Holstein follicular oocytes classified as grade A and B(50.2% and 33.2%) were higher than those of Hanwoo cattle(40.3% and 32.0%, P<0.05). The cumulus cell expansion rates of oocytes cultured in TCM-199 and Ham's F-10 medium supplemented with 10% FCS and hormones were higher (81.9~87.6%) than those of non-treated groups (74.5~81.7%). The fertilization rates of oocytes cultured in TCM-199 and Ham's F-10 medim supplemented with 10% FCS, 1% BSA and 10% bFF was 53.8~55.0%, 51.4~52.6%, and 47.0~50.0%, respectively. The polyspermy rates was 13.6~14.2%, 10.0~11.1%, and 10.0%, respectively. When the oocytes were cultured in TCM-199 and Ham's F-10 medium with 50${\mu}{\textrm}{m}$ $\alpha$-tocopherol, the fertilization rates was 62.0 and 60.2%, respectively. In the maturation medium added of 100${\mu}{\textrm}{m}$ cysteamine, the fertilization rates was 64.7 and 66.7%, respectively. The fertilization and polyspermy rates of treated groups were higher than those of non-treated group. The results show that hormones, protein sources and anti-oxidants can provide a benefit for in vitro maturation and fertilization of bovine follicular oocytes.

• Korean Journal of Veterinary Service
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• v.32 no.2
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• pp.125-129
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• 2009

SBovine tuberculosis is a chronic bacterial disease of animals and humans caused by Mycobacterium bovis. Besides the classical intradermal tuberculin test, a number of blood and serum tests have been used. The purpose of this study was to establish seroprevalence of M. bovis. The sera were screened using the ELISA technique. A total seroprevalence of 65.8% in positive cattle, suspect 36.0%, negative 5.9% in TB-infected herds by PPD and dairy cattle is 3.0%, Hanwoo is 1.6% in TB-free herds. The deer of seroprevalence is 55.0% in TB-infected herd and 7.7% in TB-free herds.

• Korean Journal of Veterinary Service
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• v.37 no.3
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• pp.157-164
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• 2014

Clostridium chauvoei is the etiologic agent of blackleg, a high mortality rated disease infection mainly cattle. In the present study, the partial sequences of 16S rRNA and flagellin gene of C. chauvoei isolated in Jeonbuk, Korea were determined and compared with those of reference strain. Oligonucleotide primers were designed to amplify a 811 bp fragment of 16S rRNA gene and 1229 bp fragment of flagellin gene. Sequencing analysis of 16S rRNA gene showed high homology to the reference strains ranging 82.3% to 100%, while flagellin gene were different from published foreign clostridia, showing 98.7% to 72.0% nucleotide sequence homology. Phylogenetic analysis based on 16S rRNA gene revealed the close phylogenetic relationship of C. chauvoei and C. septicum in cluster I, which includes C. carnis, C. tertium, C. quinii, C. celatum, C. perfringens, C. absonum, C. botulinum B. Phylogentic analysis also revealed that flagellin gene formed a single cluster with C. chauvoei, C. septicum, C. novyi A, C. novyi B, C. tyrobutylicum, C. acetobutylicum. The genetic informations obtained from this study could be useful for the molecular study of C. chauvoei.

• Journal of The Korean Society of Grassland and Forage Science
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• v.22 no.1
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• pp.45-50
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• 2002

This experiment was conducted to establish the intensive Grazing system through the improved pasture utilization of the Hanwoo beef cattle. Intensive grazing period, 2 to 3 days was compared to conventional rotation-grazing period, 5 to 6 days as control. The intensive grazing system which has 2 to 3 days of grazing period, increased forage DM yield(7.33t/ha) by 17% compared to control(6.28t/ha), and contributed to stabilization of the seasonal productivity through the increased DM yield after August. Plant height, feed intake and efficiency of grazing was increased in intensive rotation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.35 no.3
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• pp.225-231
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• 2015

Improving current pasture productivity by precision management requires practical tools to collect site specific pasture biomass data. Recent developments in unmanned aerial vehicle (UAV) technology provide cost effective and real time applications for site specific data collection. For the mapping of herbage biomass (BM) on a hill pasture, we tested a UAV system with digital cameras (visible and near-infrared (NIR) camera). The field measurements were conducted on the grazing hill pasture at Hanwoo Improvement Office, Seosan City, Chungcheongnam-do Province, Korea on May 17 and June 27, 2014. Plant samples were obtained from 28 sites. A UAV system was used to obtain aerial photos from a height of approximately 50 m (approximately 30 cm spatial resolution). Normalized digital number (DN) values of Red and NIR channels were extracted from the aerial photos and a normalized differential vegetation index using DN ($NDVI_{dn}$) was calculated. The results show that the correlation coefficient between BM and $NDVI_{dn}$ was 0.88. For the precision management of hilly grazing pastures, UAV monitoring systems can be a quick and cost effective tool to obtain site-specific herbage BM data.

• Journal of Animal Environmental Science
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• v.20 no.4
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• pp.139-146
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• 2014

This research was conducted to examine the temporal methane ($CH_4$) and nitrous oxide ($N_2O$) emission trends in livestock agriculture from year 1990 to 2011 with Tier 1 national greenhouse gas (GHG) inventory reporting method, which was related to efforts of decreasing GHG emissions and to achievement of voluntary GHG mitigation target. Methane emissions from enteric fermentation were calculated with default $CH_4$ emission factors of IPCC. Methane and $N_2O$ emissions from manure treatment processes were calculated with Tier 1 and mixture of Tier 1 and Tier 2 including $N_2O$ emission factors of manure treatment systems and nitrogen excretion rate of livestock, respectively. According to 2013 National GHG Inventory Monitoring, Reporting, and Verification report, GHG emission fluctuations from enteric fermentation and manure treatment processes were similarto livestock head fluctuation. GHG emissions from enteric fermentation were mainly affected by beef cattle including Hanwoo, while manure treatment processes were affected by various livestock.

• Korean Journal of Veterinary Service
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• v.31 no.1
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• pp.79-86
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• 2008

In this study two stage investigation was used for seroprevalence of Neospora caninum in cattle between January, 2005 and November, 2007; first stage was to examine positiveness of the farms selected randomly, second was to test all individual cow in positive farms. A total of 850 sera were collected from 170 farms for the first stage, and positive rate of farm and head were 23.5% (40/170farms) and 7.5% (64/850heads), respectively. Seroprevalence of Holstein cattle was much higher than that of Hanwoo. In second stage positive rate of cow was 18.9% (246/1,303head), but seroprevalence of farm was very variable (1 - 90.5%). It was supposed that 3 farms with high positive rate had some risk factors to be infected; raising dogs in the same farm, location of hill-side where is easy to contact with wild animals.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.3
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• pp.579-585
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• 1999

Proteins from the loin tissues age ranged from 0 to 24 months of ten Korean cattle were extracted, separated and compared on two dimensional(2 D) gels to identify the proteins whose expression is highly correlated to marbling. We also compared the difference of loin proteins between castrated and non castrated bull cows on two dimensional gels. As the marbling in the loin of the cattle is optimized at 18 to 24 months, eight proteins expressed significantly higher level in 24 month than in 0 or 6 month were selected in terms of isoelectric points(pIs) and molecular weights. Using these values, we searched the Swiss Prot database via the ExPASy molecular biology server with TagIdent program. The proteins with the nearest molecular weights and isoelectric points were selected from the lists. These possible candidates were confirmed by N terminal microsequencing of the eight selected proteins. Three proteins, myoglobin, hemoglobin and ATPase, whose N termini were not blocked could be microsequenced and found to be exactly matched to the selected candidates. It is suggested that the proteins increasingly expressed in marbling periods can be involved in meat color, lipid transport and flavor improvement.

• Journal of the Korean Data and Information Science Society
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• v.23 no.3
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• pp.467-474
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• 2012

Human diseases and livestock economic traits are not typically the result of variation of a single genetic locus, but are rather the result of interplay between interactions among multiple genes and a variety of environmental exposures. We have used linear regression model for adjusted environmental effects and multifactor dimensionality reduction (MDR) method to identify gene-gene interaction effect of statistical model in general. Of course, we use 5 SNPs (single uncleotide polymorphism) which were studied recently by Oh et al. (2011). We apply the MDR (multifactor demensionality reduction) method on the identify major interaction effects of single nucleotide polymorphisms responsible for economic traits in a Korean cattle population.

• Journal of Embryo Transfer
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• v.30 no.1
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• pp.17-21
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• 2015

Alteration in ion channel or transporter expression levels affects cell volume which is produced by movement of water and ion across the plasma membrane. In particular, aquaporin (AQP) channels among ion channels play a crucial role in movement of water across the cell membrane. This study was performed to identify whether AQP expression is changed in bovine follicular cystic follicles using microarray, RT-PCR and Western blotting analyses. In microarray data, AQP4 expression was decreased, whereas AQP7 was increased in cystic follicles. Additional experiments were focused on the AQP7 expression increased in cystic follicles. The microarray data was confirmed by semi-quantitative polymerase chain reaction (PCR) and Western blot analysis. AQP7 mRNA and protein expressions were significantly increased in the cystic follicles (p<0.05). Application of estrogen ($10{\mu}g/ml$) to bovine ovarian cells showed a trend of increase in AQP7 expression. From these results, we suggest that the increase in AQP7 expression in cystic follicles may play an important role in movement of water in bovine ovary. In addition, AQP7, a aquaglyceroporin permeating water and glycerol, could be a good target in development of methods for the cryopreservation of bovine ovary.