• 농업과학연구
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• 제48권4호
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• pp.1003-1013
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• 2021

Thioredoxin (TRX) protein is an antioxidant responsible for reducing other proteins by exchanging cysteine thiol-disulfide and is also known for its anti-allergic and anti-aging properties. On the other hand, epidermal growth factor (EGF) is an important material used in the cosmetics industry and an essential protein necessary for dermal wound healing facilitated by the proliferation and migration of keratinocytes. EGF also assists in the formation of granulation tissues and stimulates the motility of fibroblasts. Hence, genetically modified soybeans were developed to overexpress these industrially important proteins for mass production. A single-dose oral-toxicity-based study was conducted to evaluate the potential toxic effects of TRX and EGF proteins, as safety assessments are necessary for the commercial use of seed-specific protein-expressing transgenic soybeans. To achieve this rationale, TRX and EGF proteins were mass purified from recombinant E. coli. The single-dose oral-toxicity tests of the TRX and EGF proteins were carried out in six-week old male and female Institute of Cancer Research (ICR) mice. The initial evaluation of the single-dose TRF and EGF treatments was based on monitoring the toxicity signatures and mortality rates among the mice, and the resultant mortality rates did not show any specific clinical symptoms related to the proteins. Furthermore, no significant differences were observed in the weights between the treatment and control groups of male and female ICR mice. After 14 days of treatment, no differences were observed in the autopsy reports between the various treatment and control groups. These results suggest that the minimum lethal dose of TRX and EGF proteins is higher than the allowed 2,000 mg·kg^-1 limit.

• 전자공학회논문지
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• 제53권12호
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• pp.147-151
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• 2016

Standing Whole Spine 검사는 많은 병원에서 자동노출제어장치 (automatic exposure control, AEC)를 사용하고 있어 체질량지수 (body mass index, BMI)에 따라 방사선량이 조절되는 특징이 있으며, 이를 통하여 환자 별 측정 DAP (dose area product) 값을 얻을 수 있다. 하지만, 검사 시 장기의 위치에 따른 흡수선량에 관한 연구는 크게 이루어지지 않고 있으며, 이에 본 연구에서는 Standing Whole Spine 검사 시 환자의 두께정보를 대표하는 BMI와 장기의 위치에 따른 흡수선량의 분포를 평가하고자 한다. 연구의 목적을 위하여 측정된 DAP값을 이용하여 PCXMC에서 환자의 5곳의 장기를 선정 (갑상샘, 유방, 심장, 콩팥, 이자)하여 선량을 계산하였다. 결과적으로, 측정된 DAP값은 BMI에 따라 증가하는 경향을 보였지만 전방 장기인 갑상샘, 유방, 그리고 심장에서는 BMI에 따라 장기선량이 감소하는 경향을 보였다. 또한 후방장기인 콩팥과 이자에서는 BMI와 아무런 상관관계를 가지지 않았다. 결론적으로, 본 연구결과를 통하여 Standing Whole Spine 검사 시 BMI와 장기의 위치에 따라 방사선의 영향이 다르게 나타남을 증명하였다.

• Toxicological Research
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• 제23권4호
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• pp.405-413
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• 2007

This study was to investigate single and repeated-dose toxicities of Tensolin-$F^{(R)}$, an anti-wrinkle agent, in Sprague-Dawley (SD) rats or ICR mice. In single-dose oral toxicity study, the test materials were administered once by gavage to male and female SD rats at dose levels of 0 and 2,000 mg/kg. No dead animals and abnormal necropsy findings were found in control and Tensolin-$F^{(R)}$ treated group. Therefore, the approximate lethal dose of Tensolin-$F^{(R)}$ was considered to be higher than 2,000 mg/kg in rats. In the 4-week repeated oral toxicity study, the test material was administered once daily by gavage to male and female ICR mice at dose levels of 0, 25, 50 and 100 mg/kg/day for 4-weeks. In the results, no abnormality was observed in mortality, clinical findings, body weight changes, food and water consumptions, opthalmoscopic findings, necropsy findings, histopathological findings. In hematological analysis, there was a trend of increase in reticulocyte at male 25 mg/kg, although such changes were in normal ranges. On the other hand, there was a trend of decrease in hemoglobin at female 50, 100 mg/kg, such changes were in normal ranges. In addition, serum biochemical parameters including sodium, BUN and chloride increased at 25, 50 and 100 mg/kg. Relative organ weights of right testis, brain, lung and left epididymis were increased in 100 mg/kg groups of male rats in contrast to not change in female groups. However, these changes of relative organ weights, hematological and serum biochemical parameters were not accompanied with related signs such as histopathological changes or clinical findings. In conclusion, 4-week repeated oral dose of Tensolin-$F^{(R)}$ to ICR mice did not cause apparent toxicological change at the dose of 25, 50, 100 mg/kg body weight. Consequently the no-observed-adverse-effect level (NOAEL) for Tensolin-$F^{(R)}$ in ICR mice following gavage for at least 4-week is higher than 100 mg/kg/day.

• Journal of Radiation Protection and Research
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• 제39권4호
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• pp.159-167
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• 2014

국내에서는 2012년 천연방사성핵종이 포함된 가공제품의 규제를 위해 생활주변방사선 안전관리법이 시행되었지만, 해당 가공제품 사용에 대한 인체 피폭선량을 평가할 수 있는 기초자료나 피폭선량 평가기술이 미비하다. 따라서 본 연구는 사용자 피폭선량을 정량적으로 평가하기 위한 방법을 제안하고, 방사선의 종류 및 에너지에 따른 피폭선량 특성의 확인을 목적으로 한다. 피폭선량 평가를 위해서 몬테칼로 방법을 사용한 Monte Carlo N-Particle Extended (MCNPX) 코드를 통해 International Commission on Radiological Protection (ICRP)의 기준팬텀이 전산모사 되었으며, 대표적 천연방사성핵종인 우라늄 계열에서 발생되는 알파선, 베타선, 감마선의 최소, 중간, 최대 에너지가 선원항으로 사용되었다. 연간 유효선량은 가공제품 사용시간 및 사용위치를 고려한 피폭시나리오를 기반으로 평가되었다. 짧은 비정의 알파선 및 베타선은 대부분의 선량을 피부에 전달한 반면, 감마선은 대부분의 장기에 유사한 선량을 전달하였다. 방사능이 $1Bq{\cdot}g^{-1}$ 인 돌침대에 포함된 천연방사성핵종의 함유율이 10%라고 가정하고 한국인 평균 수면시간인 7시간 50분간 돌침대를 사용하였을 때 최대 연간 유효선량은 알파선, 베타선, 감마선에 대해서 각각 0.0222, 0.0836, $0.0101mSv{\cdot}y^{-1}$로 평가되었다.

• 대한방사선치료학회지
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• 제27권2호
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• pp.107-113
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• 2015

목 적 : 유방암 치료에 있어 피부선량의 측정은 매우 중요하다. 치료계획시에는 처방선량에 비해 초과선량이나 부족선량이 생길 수 있으므로 이에 대한 유방암의 여러 가지 치료계획간 피부선량 평가가 필요하다. 이에 대해 본원에서는 다양한 선량계를 이용하여 선량을 분석하여 유방암치료에 적용하고자 한다. 대상 및 방법 : 유방암은 기본적으로 접선방향 치료계획 시 일어나는 skin dose(Drain site, Scar)의 선량차이를 알아보기 위하여 인체모형팬텀을 이용하였다. 인체모형팬텀을 전산화단층촬영하고 전산화치료계획에서 open과 쐐기필터(Wedge filter)를 이용한 치료계획, Field-in-Field를 이용한 치료계획, 그리고 Dose fluence를 이용한 Irregular compensation 치료계획을 세우고 컴퓨터치료계획 프로그램(Eclipse)으로 선량관심점과 측정점의 선량을 비교하였다. 치료실에서 인체모형팬텀을 위치시키고 선량비교를 위하여 각 치료계획 측정점에 열형광선량계(themoluminesence dosimeter, TLD)와 MOSFET(Metal oxide-silicon field effect transistor)을 이용하여 선량을 측정하여 비교평가 하였다. 결 과 : 피부선량은 치료계획 중심점을 기준으로 위와 아래는 Dose fluence를 이용한 Irregular compensation 치료계획 사용 시 MOSFET을 이용한 선량측정에서 가장 많은 선량이 들어가는 것으로 나타났다. 내측과 외측의 측정선량은 open과 쐐기필터 치료계획에서 TLD와 MOSFET을 이용하여 측정시 5.7%에서 10.3%까지 낮게 나타났다. 반대쪽 유방의 선량은 open 치료계획이 가장 적었고, Dose fluence를 이용한 Irregular compensation 치료계획을 사용 시 가장 많은 선량이 측정되었다. 치료종별 치료계획상에서는 내측과 외측의 선량편차가 가장 컸으며, TLD와 MOSFET 측정시에도 같은 경향을 보였다. 외측은 TLD, 내측은 MOSFET이 가장 편차가 컸다. 결 론 : 치료계획에 따른 피부선량은 전반적으로 Dose fluence를 이용한 Irregular compensation의 치료계획을 사용 시 가장 많이 들어가는 것으로 나타났으며, 이는 많은 MLC의 움직임에 의한 산란선 영향으로 생각된다. 모든 치료계획에서 피부의 위치에 따라 약간의 차이는 있으나 부족선량이 생기는 부분에서는 내측의 내유임파절(Intramammary lymph nodes)선량이나 Scar, Drain site등에서 세심한 주의가 필요하다. 부족선량을 높이기위해서는 Dose fluence를 이용한 Irregular compensation의 치료계획을 사용하는 것이 좋겠으나, 전체적인 선량을 높이기보다는 선택적인 범위내에서 선량을 높이게 되므로 환자의 연령이나 움짐임 등을 고려하여 치료기술을 선택하는 것이 바람직할 것으로 사료된다.

• 한국환경보건학회지
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• 제35권5호
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• pp.362-364
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• 2009

The present study was undertaken to estimate the antibacterial effect of Mume Fructus water extract (MFWE) against murine salmonellosis. At MFWE concentrations ranging from 25 to 100 ${\mu}g/ml$, the antibacterial effect was not showed on Salmonella typhimurium (S. typhimurium). On the other hand, bacteria without MFWE had a tendency to proliferate up to 8 h after incubation. Oral administration of MFWE at the dose of 40 mg/ml showed a therapeutic effect for S. typhimurium infected BALB/c mice. The mortality of MFWE-treated mice was 80% at 12 days, while that of MFWE-untreated mice was 100% at 9 days after a lethal dose of S. typhimurium infection. The results of our study strongly indicate that MFWE has potential as an effective of salmonellosis.

• 생약학회지
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• 제37권3호
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• pp.212-216
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• 2006

The effect of aqueous extracts of medicinal plants $AMP-365^{TM}$ was tested for immune system regulating activity based on anti-inflammatory activity, anti-oxidant, macrophage proliferation and T-lymphocyte proliferation activity. $AMP-365^{TM}$ dose-dependently increased proliferation of RAW264.7 macrophage cells and its nitric oxide production as well as DPPH radical scavenging activity. On the other hand, T-lymphocyte proliferation activity was decreased on dose-dependent manner. Passive cutaneous anaphylaxis reaction was alleviated by 49% by administering 250 mg/kg of $AMP-365^{TM}$. The results suggest that $AMP-365^{TM}$ can be beneficial in the treatment of immediate allergic reactions as an adjuvant supplement material.

• 한국식품위생안전성학회지
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• 제11권2호
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• pp.115-121
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• 1996

In order to compare the suppressive effect of quercetin and several its glycosides, such as quercitrin (quercetin-3-rhamnoside), isoquercitrin (quercetin-3-glucoside), hyperin (quercetin-3-galactoside) and tutin (quercetin-3-rhamnosyl glucoside), on the genotoxicity by N-methyl-N-nitrosourea(MNU), in vitro sister chromatid exchange(SCE) test using mouse spleen lymphocytes and in vivo micronucleus test using mouse peripheral blood were performed. MNU-induced SCEs in vitro were not decreased by the simultaneous treatment of test compounds. Among them, quercetin and hyperin showed significant suppressive effects at high dose(10-5M). On the other hand, MNU-induced micronucleated reticulocytes(MNRETS) in vivo were significantly decreased with good dose-dependent manner in all compound tested. However, there were not significant differences between quercetin aglycone and its glycosides in the suppressive aglycone and its glycosides may act as an antigenotoxic agent in vivo and may be useful as a chemopreventive agent of alkylating agent.

• Biomolecules & Therapeutics
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• 제6권3호
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• pp.242-246
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• 1998

The inhibitory activities of Amberlite active fraction, which was obtained from methanol soluble fraction of freeze dried slikworm urine, on the rat intestinal glycosidase-catalyzed enzymatic reaction were examined in in viro and in vivo experiments. Amberlite active fraction showed significant inhibitory effects on the hydrolysis of o-glycosidic bond, especially $\alpha$-1,4 bond. On the other hand, the inhibition on the hydrolysis of $\beta$-glycosidic bond was very weak. Oral administration of Amberlite active fraction resulted in a dose-dependent decrease in the blood glucose after an oral maltose load, and postprandial hyperglycemia in carbohydrate-loaded mice was suppressed by Amberlite active fraction at 60 mgHg in decreasing order of maltose, starch, sucrose and lactose. 60 mg/kg of Amberlite active fraction lowered the blood glucose level markedly after 18, 35, and 60 min after an oral maltose load and the antihyperglycemic activity was maintained upto 90 min. In alloxan-induced hyperglycemic mice, Amberlite active fraction at a dose of 100 mg/kg also significantly lowered blood glucose after an oral maltose load, and its efficacy was almost equivalent to that of acarbowe.

• Biomolecules & Therapeutics
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• 제4권3호
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• pp.232-238
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• 1996

The physical dependence potency of DA-5018, a non-narcotic analgesic agent, was tested in mice dosed with 0.5 and 4 mg/kg/day for 2 months and daily increasing doses of 1, 2, 4, 6, 8 and 10 mg/kg over 10 days. Physical dependence was assessed taking natural withdrawal induced morphine-type abstinence (jumping, falling, biting or backward locomotion, rearing etc.) as well as barbiturates-type abstinence (body weight reduction, convulsion, ataxia etc.) into consideration. The results were compared with those after the same daily increasing doses of morphine. DA-5018 did not show evidence of physical dependence liability or abuse potential as measured by morphine-type or barbiturate-type abstinence signs following daily increasing or 2-month repeated administration. On the other hand, daily increasing doses of morphine produced physical dependence and the dependent state disappeared about 6 hours after the start of withdrawal signs. In the single dose suppression test, a single dose of morphine completely suppressed natural withdrawal signs that appeared in morphine-dependent animals. Therefore, these results indicate that DA-5018 does not have abuse potential and physical dependence liability.