• Title/Summary/Keyword: Half-Cell

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A Study on Corrosion Estimates of Steel in Mortar Accelerated under the Environment of Artificial Seawater (인공해수(人工海水)에서 촉진시험(促進試驗)한 모르터 중의 철근부식(鐵筋腐蝕) 평가(評價)에 대한 연구(研究))

  • Moon, Han Young;Kim, Seong Soo
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.13 no.5
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    • pp.67-75
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    • 1993
  • This study was performed for the purpose of estimating steel corrosion and of considering a countermeasure to prevent steel corrosion of concrete structures under the environment of seawater or using seasand. The corrosion of steel was accelerated in artificial seawater with seven kinds of specimen which was embedded steel in mortar. To assume the degree of steel corrosion, soluble chloride content in mortar, $Cl^-$ binding capacity, half cell potential and corrosion area ratio were measured. The results show that corrosion area ratio was correlative with half cell potential and soluble chloride content in mortar.

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Novel AGLP-1 albumin fusion protein as a long-lasting agent for type 2 diabetes

  • Kim, Yong-Mo;Lee, Sang Mee;Chung, Hye-Shin
    • BMB Reports
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    • v.46 no.12
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    • pp.606-610
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    • 2013
  • Glucagon like peptide-1 (GLP-1) regulates glucose mediated-insulin secretion, nutrient accumulation, and ${\beta}$-cell growth. Despite the potential therapeutic usage for type 2 diabetes (T2D), GLP-1 has a short half-life in vivo ($t_{1/2}$ <2 min). In an attempt to prolong half-life, GLP-1 fusion proteins were genetically engineered: GLP-1 human serum albumin fusion (GLP-1/HSA), AGLP-1/HSA which has an additional alanine at the N-terminus of GLP-1, and AGLP-1-L/HSA, in which a peptide linker is inserted between AGLP-1 and HSA. Recombinant fusion proteins secreted from the Chinese Hamster Ovary-K1 (CHO-K1) cell line were purified with high purity (>96%). AGLP-1 fusion protein was resistant against the dipeptidyl peptidase-IV (DPP-IV). The fusion proteins activated cAMP-mediated signaling in rat insulinoma INS-1 cells. Furthermore, a C57BL/6N mice pharmacodynamics study exhibited that AGLP-1-L/HSA effectively reduced blood glucose level compared to AGLP-1/HSA.

A Study on Sol-gel Preparation of Pt-Ru/C Anode Catalysts for Direct Methanol Fuel Cells (솔-젤 합성에 의한 직접 메탄올 연료전지용 고분산 Pt-Ru/C 음극 촉매의 제조)

  • Lee, Kang-Hee;Kim, Il-Gon;Park, Tae-Jin;Suh, Dong-Jin
    • Journal of the Korean Electrochemical Society
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    • v.6 no.1
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    • pp.13-17
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    • 2003
  • Cryogel and aerogel Pt-Ru/C were synthesized by the sol-gel process for the electrooxidation of methanol. From XRD analysis, it was found that the catalysts had highly dispersed Pt-Ru alloys on carbon support although high temperature treatments have been conducted. Electrocatalytic activities of 3 type aerogel catalysts were investigated in half cell experiments by cyclic voltammetry. Among them, Phloroglucinol-Formaldehyde(PF) type catalyst shows the highest activity. From the results of deactivation test for each catalysts, the aerogel catalysts are found to have excellent durability compared with those prepared by colloidal method.

Ceramide-Mediated c-jun Gene Expression in U-937 Cells (U-937 세포에 있어서 세라마이드에 의한 c-jun 유전자 발현의 조절)

  • Kim, Won-Ho;Kim, Mie-Young;Choi, Kyung-Hee
    • YAKHAK HOEJI
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    • v.41 no.1
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    • pp.81-85
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    • 1997
  • Ceramide has been suggested as an important mediator of the effects of extracellular agonists on cell growth inhibition, differentiation, apoptosis. However the biochemical sign aling mechanism involved in transducing the effects of ceramide on leukemia cell differentiation is still unclear. In these respects, we examined the regulatory effects of ceramide on c-jun gene expression during differentiation. In U-937 cells. ceramide increased c-jun mRNA levels in a time-dependent manner. The half life, of c-jun mRNA was 30 min. In contrast, inhibition of protein synthesis with cycloheximide in the absence, of transcription with actinomycin D increased the half-life of c-jun mRNA in ceramide-treated U-937 cells to more than 90 min. In order to examine whether ceramide-inhibited c-jun gene expression is regulated through ceramide-activated protein phosphatase (CAPP), a direct target for the action of ceramide, okadaic acid were treated to the cells. Okadaic acid inhibited enhancement of c-jun mRNA induced by C2-ceramide in a dose-dependent manner. These results suggested that ceramide increases c-jun mRNA level during differentiation in U-937 cells and regulates the gene expression on posttranscriptional level. In addition, we provide the evidence that CAPP is involved in ceramide-induced c-jun gene expression in U-937 cells.

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Prediction of Lithium Diffusion Coefficient and Rate Performance by using the Discharge Curves of LiFePO4 Materials

  • Yu, Seung-Ho;Park, Chang-Kyoo;Jang, Ho;Shin, Chee-Burm;Cho, Won-Il
    • Bulletin of the Korean Chemical Society
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    • v.32 no.3
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    • pp.852-856
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    • 2011
  • The lithium ion diffusion coefficients of bare, carbon-coated and Cr-doped $LiFePO_4$ were obtained by fitting the discharge curves of each half cell with Li metal anode. Diffusion losses at discharge curves were acquired with experiment data and fitted to equations. Theoretically fitted equations showed good agreement with experimental results. Moreover, theoretical equations are able to predict lithium diffusion coefficient and discharge curves at various discharge rates. The obtained diffusion coefficients were similar to the true diffusion coefficient of phase transformation electrodes. Lithium ion diffusion is one of main factors that determine voltage drop in a half cell with $LiFePO_4$ cathode and Li metal anode. The high diffusion coefficient of carbon-coated and Cr-doped $LiFePO_4$ resulted in better performance at the discharge process. The performance at high discharge rate was improved much as diffusion coefficient increased.

Evaluation on Steel Bar Corrosion Embedded in Antiwashout Underwater Concrete

  • Moon Han-Young;Shin Kook-Jae
    • Journal of the Korea Concrete Institute
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    • v.17 no.2 s.86
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    • pp.303-309
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    • 2005
  • This study aims the evaluation of the corrosion of steel bar embedded in antiwashout underwater concrete, which has rather been neglected to date. To that goal, accelerated steel bar corrosion tests have been performed on three series of steel bar-reinforced antiwashout underwater concrete specimens manufactured with different admixtures. The three series of antiwashout underwater concrete were: concrete constituted exclusively with ordinary portland cement (OPC), concrete composed of ordinary portland cement mixed with fly-ash in $20\%$ ratio (FA20), and concrete with ground granulated blast furnace slag mixed in $50\%$ ratio (BFS50). The environment of manufacture was in artificial seawater. Measurement results using half-cell potential surveyor showed that, among all the specimens, steel bar in OPC was the first one that exceeded the threshold value proposed by ASTM C 876 with a potential value below -350mv after 14 cycles. And, the corresponding corrosion current density and concentration of water soluble chloride were measured as $30{\mu}A/mm^2$ and $0.258\%$. On the other hand, for the other specimens that are FA20 and BFS50, potential values below -350mV were observed later at 18 and 20 cycles, respectively. Results confirmed the hypothesis that mineral admixtures may be more effective on delay the development of steel bar corrosion in antiwashout underwater concrete.

Influence of Loading on the Corrosion of Reinforcing Bar (철근콘크리트 보의 철근부식에 미치는 하중의 영향에 관한 연구)

  • 김형래;윤상천;지남용
    • Journal of the Korea Concrete Institute
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    • v.11 no.3
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    • pp.171-179
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    • 1999
  • The present research investigated the interaction among loading level, corrosion rate and flexural deflection of reinforced concrete beams. 10cm$\times$15cm$\times$110cm reinforced concrete beams were prepared and subjected to different levels of flexural loading, including 0%, 45% and 75% of the ultimate load. The beams with either a pre-load or a sustained load were also exposed to a laboratory environment with ponding and wetting/drying cycling at room temperature. Half cell potential and galvanized current measurements were taken to monitor corrosion process of reinforcing steel. After corrosion initiation, external current was applied to some of the beams to accelerate corrosion propagation. The beam deflections were recorded during the entire tests. The results indicate that loading level has significant effect on corrosion rate. The beams under a sustained load had much higher corrosion rate than the pre-loaded and then unloaded beams. Significant corrosion may result in an increase in beam deflection and affect serviceability of the structure. The present research may provide an insight into structural condition evaluation and service life predictions of reinforced concrete.

A Study on the Somatic Cells in Half Milk Samples of Dairy Goats (젖염소 분방유즙에서 체세포에 대한 연구)

  • Kim, Hye-Ra;Lee, Jeong-Chi;Jeong, Ji-Young;Lee, Youn-Kyung;Shin, Sung-Shik;Lee, Chai-Yong
    • Journal of Veterinary Clinics
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    • v.24 no.2
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    • pp.177-181
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    • 2007
  • In the present study, a total of 547 half milk samples were collected from 274 dairy goats to perform somatic cell counts (SCC) and California mastitis test (CMT). Milk smear was stained with Pyronin Y-Methyl Green stain were classified into either epithelial or blood cells, etc. Of the 547 halves the percentage of CMT negative milk samples were 86%. Among these, 58.2% were CMT negative with SCC<500,000/ml, while 27.8% were CMT negative with SCC>500,000 ml. As expected, CMT score increased with the increase of SCC. The number of epithelial cells decreased with the increasing number of somatic cells, while the opposite was observed with the number of blood cells. These results indicate that the critical point in milk quality & CMT should be considered on the false (pseudo-SC) SCC in dairy goat.

Experimental Investigation of Electrochemical Corrosion and Chloride Penetration of Concrete Incorporating Colloidal Nanosilica and Silica Fume

  • Garg, Rishav;Garg, Rajni;Singla, Sandeep
    • Journal of Electrochemical Science and Technology
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    • v.12 no.4
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    • pp.440-452
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    • 2021
  • Enhancement of durability and reduction of maintenance cost of concrete, with the implementation of various approaches, has always been a matter of concern to researchers. The integration of pozzolans as a substitute for cement into the concrete is one of the most desirable technique. Silica fume (SF) and colloidal nanosilica (CS) have received a great deal of interest from researchers with their significant performance in improving the durability of concrete. The synergistic role of the micro and nano-silica particles in improving the main characteristics of cemented materials needs to be investigated. This work aims to examine the utility of partial substitution of cement by SF and CS in binary and ternary blends in the improvement of the durability characteristics linked to resistance for electrochemical corrosion using electrical resistivity and half-cell potential analysis and chloride penetration trough rapid chloride penetration test. Furthermore, the effects of this silica mixture on the compressive strength of concrete under normal and aggressive environment have also been investigated. Based on the maximum compression strength of the concrete, the optimal cement substituent ratios have been obtained as 12% SF and 1.5% CS for binary blends. The optimal CS and SF combination mixing ratios has been obtained as 1.0% and 12% respectively for ternary blends. The ternary blends with substitution of cement by optimal percentage of CS and SF exhibited decreased rate for electrochemical corrosion. The strength and durability studies were found in consistence with the microstructural analysis signifying the beneficiary role of CS and SF in upgrading the performance of concrete.

Ex-situ conservation and cytotoxic activity assessment of native medicinal orchid: Coelogyne stricta

  • Thapa, Bir Bahadur;Thakuri, Laxmi Sen;Joshi, Pusp Raj;Chand, Krishna;Rajbahak, Sabari;Sah, Anil Kumar;Shrestha, Resha;Paudel, Mukti Ram;Park, So Young;Pant, Bijaya
    • Journal of Plant Biotechnology
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    • v.47 no.4
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    • pp.330-336
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    • 2020
  • Ex-situ conservation of the ornamental and medicinal orchid, Coelogyne stricta, was performed by mass propagation using seed culture. Propagation stages were optimized using full- and half-strength solidified MS medium with different phytohormones. Maximum seed germination (88 ± 0.5% over 6 weeks of culture) was achieved on half-strength MS medium supplemented with 15% coconut water. Maximum shoot numbers were found on full-strength MS medium supplemented with 1 mg/L BAP, 2 mg/L Kinetin, and 10% coconut water, while the longest root was developed on full-strength MS medium with 1.5 mg/L IBA. A 2:1:1 combination of coco-peat, pine bark, and sphagnum moss was found to be a suitable potting mixture resulting in 80% seedling survivability. The cytotoxic activity of extracts of both wild plants and in vitro-developed protocorms was determined using an MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) assay on a cervical cancer cell line. The wild plant extract inhibited the growth of 41.99% of cells, showing that this extract has moderate cytotoxic activity toward cervical cancer cells.