• Korean Journal of Soil Science and Fertilizer
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• v.49 no.2
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• pp.181-193
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• 2016

This experiment was conducted to evaluate the effect of carbonized rice hull (CRH) application on soil carbon storage and $N_2O$ emissions from upland soil. It was used at different rates of 0, 5, 10 and $20Mg\;ha^{-1}$. During the Chinese cabbage cultivation, several soil chemical characteristics such as soil moisture, temperature and soil carbon were observed. Also, $CO_2$ and $N_2O$ emissions were monitored. Soil organic matter contents slightly increased with carbonized rice hull applied in all the treatments. The soil carbon contents with application rate of 0, 5, 10 and $20Mg\;ha^{-1}$ were 0, 1.3, 1.2 and $2.6g\;kg^{-1}$, respectively. It was observed that soil carbon content was higher with increasing CRH application rate. Total nitrogen contents of soil applied with CRH relatively decreased with the course of time. However, $NO_3$-N contents in the soil with CRH application rate of 5, 10 and $20Mg\;ha^{-1}$ were 28.6, 25.7 and $21.5mg\;kg^{-1}$ at the end of experiment, respectively. $CO_2$ emission at the $5Mg\;ha^{-1}$ application of CRH was higher about 18.9% than non-treatment, whereas those of $10Mg\;ha^{-1}$ and $20Mg\;ha^{-1}$ treatment were lower 14.4% and 11.8% compared to non-treatment, respectively. Also, it was shown that $N_2O$ emission reduced by 19.9, 28.3 and 54.0% when CRH was applied at 5, 10 and $5Mg\;ha^{-1}$, respectively.

• Korean Journal of Soil Science and Fertilizer
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• v.33 no.3
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• pp.205-211
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• 2000

This study was conducted to evaluate the residual effects of basic oxygen furnace (BOF) slag applied in rice paddy fields as soil conditioner one year before. The experimental fields of Lim et al. (2000) located in Youjung and Nampyung were used for this purpose. Both variety (Oryza sativa L. cv. Dongjinbyeo) and cultural practices were the same as those in Lim et al. (2000). Soil chemical properties, plant height, number of tillers per plant, yield and yield components were observed. The temporal variation of treatment mean value in soil chemical properties appeared to be similar trends in both Youjung and Nampyung experimental fields. Soil pH and Ca content were still significantly higher than those in control treatment up to July of the second season, but decreased progressively as time passed. However, the effects lasted longer as slag rate became higher. BOF slag seems to have residual effects as a soil conditioner or Ca fertilizer in soil for two years. BOF slag rate of $4Mg\;ha^{-1}$ raised soil pH almost the same as lime rate of $2Mg\;ha^{-1}$. Content of $SiO_2$ in soil applied slag appeared to be higher compared with control. Fe and Mg content in soil with slag treatment was significantly higher than that of control in 1997, but it was almost the same level as that of control in 1998. In YouJung experimental field, rough rice yield of slag teatment became higher as slage rate incresed. Slag rate of $12Mg\;ha^{-1}$ showed the highest rough rice yield of $5,400kg\;ha^{-1}$ among treatment, which was 14% higher than that of control with $4,720kg\;ha^{-1}$. Slag rate of $12Mg\;ha^{-1}$ showed relatively higher plant height and higher number of tillers at the early growth stage compared with other treatments. In NamPyung experimental field, rough rice yield was the highest at the plot of lime rate $2Mg\;ha^{-1}$ and became higher as slag rate increased. There were no significant differences in rough rice yield between lime treatment and slag treatments. Slag rate of $12Mg\;ha^{-1}$ showed the highest rough rice yield of $7,170kg\;ha^{-1}$ among slag treatment, which was 8% significantly higher than that of control with $6,670kg\;ha^{-1}$. Slag rate of $12Mg\;ha^{-1}$ showed relatively slower growth in plant height at the early growth stage, but superior growth at the later growth stage, and significantly higher number of spiklets per panicle and 1000-grain weight than that of control.

• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.2
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• pp.106-110
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• 1985

This trial was carried out to find out the optimum ridge and seeding rate for the seed production of Festuca arundinacea S. (variety Alta). It was composed of three ridge level (12, 18 and 24cm) and four seeding rates (10, 15, 20 and 25kg/ha) in a split-plot design with 4 replications. The experiment lasted from September 1979 to October 1981. 1. The beginning of heading state was May 12 and the date of full flowering stage was June 2. The optimum stage for the seed harvesting of Festuca arundinacea was about 43 days after beginning of heading stage. 2. The number of panicles were 157 per square meter from the best treatment 18cm ridge with 15kg seed/ha. And the 1000 grain weight was 2.79g for the treatment 12cm ridge with 10kg seed/ha. 3. The average seed yield for two years was 681kg/ha and the largest 831kg/ha for the treatment 18cm ridge with 25kg seed/ha. 4. the average germination rate of the harvested seed was 90.7 percent, and it showed a little higher germination rate from the lower level of seeding rate. 5. The average total DM production with two cuttings of aftermath was about 6715kg/ha. According to the increasing seeding rate and narrowing the ridge, DM yield was higher.

• Journal of Korea Soil Environment Society
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• v.1 no.1
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• pp.11-17
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• 1996

Trichloroethylene(TCE) is the organic compound which is used variously at the industrial areas. It contaminates soils and groundwater by leaked storage tank, careless treatment in field and the effluent from waste landfills. This study was carried out to identify adsorptive behavior of TCE by soil. Batch experiments were conducted at different soil-organic matter content and lime treatment to determine Freundlich isothermal adsorption equation constant, k and n, for TCE. Sewage sludge cake was applied to make different soil-organic matter content with the level of Oton/ha(S1), 50ton/ha(S2), 100ton/ha(S3). Lime(calcium hydroxide) was treated with the level of 2ton/ha, 4ton/ha, 6ton/ha, 10ton/ha. Freundlich isothermal adsorption equations obtained from experiment with sewage sludge cake were as follows (on condition that the level of TCE applied to soil ranged from 0.5ng/g soil to 2.5 ng/g soil.) : S1 :x/m = 0.393 $C^2$, S2 : x/m = 0.436 $C^2$, S3 : x/m = 0.636 $C^2$Value of k was increased in higher order of 51, 52, 53 with increased level of sewage sludge cake application. From this results, soil which was applied higher level of sewage sludge cake had a good ability on TCE adsorption. With increased the level of lime application, pH of the soil was increased and the ability of the soil in TCE adsorption was decreased.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.50 no.2
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• pp.105-107
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• 2005

A field study was conducted to investigate and ascertain the effects of boron and molybdenum with recommended N P K S Zn fertilizers (RFD) for sustained sugarcane yield in the sandy acidic soil in Old Himalayan Piedmont Plain of Bangladesh. Results revealed that the treatment $T_7$ having recommended fertilizers of N 120, P 35, K 100, S 25 and Zn 2kg $ha^{-1}$ + B 2kg $ha^{-1}$ + Mo 2 kg $ha^{-1}$ produced significantly higher number of millable cane (126.96\times10^3\;ha^{-1})$ except in $T_9$ with RFD + B 2 kg $ha^{-1}$ + Mo 4kg $ha^{-1}$, and higher cane yield (92.83 Mg $ha^{-1}$) among all except in $T_3$ with RFD + B2 kg $ha^{-1}$, $T_5$ with RFD + Mo 2kg $ha^{-1}$ and $T_9$ where the effects were statistically identical. The increase in cane yield by $T_7$ was $164.6\%$ over control. The treatment $T_7$ having RFD + B 2kg $ha^{-1}$ + Mo 2kg $ha^{-1}$ further gave the highest additional net economic benefit of Taka 16, 500.00 for added Band Mo at 2kg $ha^{-1}$ among all the treatments in the study.

• Biomolecules & Therapeutics
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• v.29 no.5
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• pp.536-544
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• 2021

5-amino salicylic acid (5-ASA) is a standard therapy for the treatment of mild to moderate forms of inflammatory bowel diseases (IBD) whereas more severe forms involve the use of steroids and immunosuppressive drugs. Hyaluronic acid (HA) is a naturally occurring non-sulfated glycosaminoglycan that has shown epithelium protective effects in experimental colitis recently. In this study, both 5-ASA (30 mg/kg) and HA (15 mg/kg or 30 mg/kg) were administered rectally and investigated for their potential complementary therapeutic effects in moderate or severe murine colitis models. Intrarectal treatment of moderate and severe colitis with 5-ASA alone or HA alone at a dose of 30 mg/kg led to a significant decrease in clinical activity and histology scores, myeloperoxidase activity (MPO), TNF-α, IL-6 and IL-1β in colitis mice compared to untreated animals. The combination of HA (30 mg/kg) and 5-ASA in severe colitis led to a significant improvement of colitis compared to 5-ASA alone. Combined rectal therapy with HA and 5-ASA could be a treatment alternative for severe cases of IBD as it was the only treatment tested that was not significantly different from the healthy control group. This study further underlines the benefit of searching for yet unexplored drug combinations that show therapeutic potential in IBD without the need of designing completely new drug entities.

• The Korean Journal of Ecology
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• v.18 no.2
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• pp.219-230
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• 1995

Although many studies of nutrient cycling in forest ecosystems have reported that clearcutting creates increased organic matter decomposition and nitrogen (N) mineralization in soils, little is known about the change of these factors following various levels of canopy removal. A series of experimental plots with four levels of canopy cover, i.e., clearcut, 25％, 75％, and uncut, was established in northern red oak (Quercus rubra L.) stands in northern Lover Michigan, U.S.A. I examined decomposition of cellulose filter papers and N mineralization using an in situ soil incubation technique in the top 15cm of mineral soil during the second growing season (1992, May-October) following stand manipulation. Mass loss from cellulose filter papers was more rapid in the canopy removal treatments than in the uncut treatment. similarly, net N mineralization was significantly greater in the canopy removal treatments than in the uncut treatment. There was no significant difference in net N mineralization rates among the three levels of canopy removal. Net N mineralization for the growing season was 58 kg/ha for the clearcut, 54 kg/ha for the 25％ canopy cover, 51 kg/ha for the 75％ canopy cover, and 22 kg/ha for the uncut treatment. These results indicated that even only small amounts of canopy removal (leaving 75％ canopy cover) let to substantial increases of cellulose decomposition and the amount of available soil nitrogen.

• Korean Journal of Materials Research
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• v.14 no.9
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• pp.607-613
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• 2004

This study was to investigate the surface properties of electrochemically oxidized Ti-6Al-7Nb alloy by anodic spark discharge technique. Anodizing was performed at current density 30 $mA/cm^2$ up to 300 V in electrolyte solutions containing $DL-{\alpha}$-glycerophosphate disodium salt hydrate($DL-{\alpha}$-GP) and calcium acetate (CA). Hydrothermal treatment was done at $300^{\circ}C$ for 2 hrs to produce a thin outermost layer of hydroxyapatite (HA). The bioactivity was evaluated from HA formation on the surfaces in a Hanks' solution with pH 7.4 at $36.5^{\circ}C$ for 30 days. The size of micropores and the thickness of oxide film increased and complicated multilayer by increasing the spark forming voltage. Needle-like HA crystals were observed on anodic oxide film after the hydrothermal treatment at $300^{\circ}C$ for 2 hrs. When increasing $DL-{\alpha}$-GP in electrolyte composition, the precipitated HA crystals showed the shape of thick and shorter rod. However, when increasing CA, the more fine needle shape HA crystals were appeared. The bioactivity in Hanks' solution was accelerated when the oxide films composed with strong anatase peak with presence of rutile peak. The increase of amount of Ca and P was observed in groups having bioactivity in Hanks' solution. The Ca/P ratio of the precipitated HA layer was equivalent to that of HA crystal and it was closer to 1.67 as increasing the immersion time in Hanks' solution.

• The Korea Journal of Herbology
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• v.34 no.1
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• pp.51-57
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• 2019

Objectives : Nypa fruticans Wurmb. (NF) have been used as a traditional medicine to treat inflammatory diseases in East-South Asia. However, it is largely undiscovered whether NF water extract could exhibit anti-inflammatory activities against tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$)-induced inflammatory responses on human keratinocytes, HaCaT cells. Therefore, this study was aimed to investigate the anti-inflammatory activity of NF water extract on TNF-${\alpha}$-induced inflammatory responses in HaCaT cells. Methods : To investigate the anti-inflammatory activites of NF water extract in HaCaT cells, the inflammatory model of HaCaT cells was established under a suitable concentration (10 ng/ml) of human TNF-${\alpha}$ (hTNF-${\alpha}$). HaCaT keratinocyte cells were pre-treated with NF water extract for 1 h, and then stimulated with hTNF-${\alpha}$. Then, the cells were harvested to measure the inflammatory mediators such as inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 and prostaglandin $E_2$ ($PGE_2$), and pro-inflammatory cytokine including TNF-${\alpha}$ and interleukin (IL)-6. In addition, we examined the inhibitory mechanisms of NF, mitogen activated protein kinases (MAPKs) and inhibitory kappa B alpha ($I{\kappa}-B{\alpha}$) Results : The treatment of NF inhibited the hTNF-${\alpha}$-induced elevation of iNOS, COX-2, and $PGE_2$ in HaCaT cells. In addition, NF treatment inhibited the hTNF-${\alpha}$-induced elevation of TNF-${\alpha}$ and IL-6. Furthermore, NF treatment inhibited the activation of MAPKs but not degradation of $I{\kappa}-B{\alpha}$. Conclusions : Taken together, our result suggest that treatment of NF could inhibit the hTNF-${\alpha}$-induced inflammatory responses via deactivation of MAPKs in HaCaT cells. This study could suggest that NF could be a beneficial agent to prevent skin damage or inflammation.

• The Korea Journal of Herbology
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• v.28 no.4
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• pp.57-62
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• 2013

Objectives : Lonicerae Japonicae Flos (LJF) has been shown anti-oxidant, anti-inflammatory, anti-viral, anti-rheumatoid properties. However, it is still largely unknown whether LJF inhibits skin injury against oxidative stress in human keratinocyte, HaCaT cells. The purpose of this study was to evaluate the protective effects of LJF against hydrogen peroxide($H_2O_2$)-induced oxidative stress in human keratinocytes, HaCaT cells. Methods : To evaluate out the protective effects of LJF on oxidative injury in HaCaT cells, an oxidative stress model of HaCaT cells was established under a suitable concentration (500 ${\mu}M$) hydrogen peroxide. HaCaT keratinocyte cells were pre-treated with LJF (0.1, 0.25 or 0.5 mg/ml), and then stimulated with $H_2O_2$. Then, the cells were harvested to measure the cell viability, DNA damage, and release of reactive oxygen species (ROS). Results : LJF (0.1, 0.25 or 0.5 mg/ml) itself did not show any significant toxicity in HaCaT cells. The treatment of $H_2O_2$ caused the oxidative stress, leading to the cell death, and DNA injury. However, pretreatment with LJF reduced cell death, and DNA injury. The stimulation of $H_2O_2$ on HaCaT cells resulted in excessive release of ROS, which is the main factor of oxidative stress. The excessive release of ROS was inhibited by LJF treatment significantly. Conclusions : These results could suggest that LJF exhibited the protective effects of HaCaT cells against $H_2O_2$-induced oxidative stress by inhibiting ROS release. It could be explained that LJF inhibit skin damages against oxidative stress. Thus, LJF would be useful for the development of drug or cosmetics treating skin troubles.