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Correlation Analysis of Inspection Results and ATP Bioluminescence Assay for Verification of Hygiene Status at 5 Star Hotels in Korea (국내 주요 5성급 호텔의 위생실태 조사와 ATP 결과의 상관분석 평가 연구)

  • Kim, Bo-Ram;Lee, Jung-A;Ha, Sang-Do
    • Journal of Food Hygiene and Safety
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    • v.36 no.1
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    • pp.42-50
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    • 2021
  • Along with the rapid growth of the food service industry, food safety requirements and hygiene are increasing in importance in restaurants and hotels. Accordingly, there is a need for quick and practical monitoring techniques to determine hygiene status in the field. In this study, we investigated 5 domestic 5-star hotels specifically, personal hygiene (hands of workers), cooking utensils (knife, cutting board, food storage container, slicing machine blade, ice-maker scoop) and other facilities (refrigerator handle, sink). In addition, we examined the hygiene management status of customer contact points (tongs for buffet, etc.) to derive the correlation between the ATP values as a, a verification method. As a result of our five-hotel survey, we found that cooking utensils and personal hygiene were relatively sanitary compared to other inspection items (cookware 92.2%, personal hygiene 91.4%, facilities and equipment 76.19%, customer contact items 88.6%). According to our ATP-based mothod, kitchen utensils (51 ± 45 RLU/25㎠) were relatively clean compared to other with facilities and equipment (167 ± 123 RLU/25㎠). In the present study, we also evaluated the usefulness of the ATP bioluminescence method for monitoring surface hygiene at hotel restaurants. After correlation analysis of surveillance of hygienic status points and ATP assay, most results showed negative and high correlation (-0.64--0.89). Our ATP assay (92 ± 67 RLU/25㎠) of each item after cleaning showed signigicantly reduced results compared to the ATP assay (1020 ± 1254 RLU/25㎠) for normal status, thereby indicating its suitability as a tool to verify the validity of cleaning. By our results, ATP bioluminescence could be used as an effective tool for visual numerical evaluation of invisible contaminants.

Performance of a Molten Carbonate Fuel Cell With Direct Internal Reforming of Methanol (메탄올 내부개질형 용융탄산염 연료전지의 성능)

  • Ha, Myeong Ju;Yoon, Sung Pil;Han, Jonghee;Lim, Tae-Hoon;Kim, Woo Sik;Nam, Suk Woo
    • Clean Technology
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    • v.26 no.4
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    • pp.329-335
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    • 2020
  • Methanol synthesized from renewable hydrogen and captured CO2 has recently attracted great interest as a sustainable energy carrier for large-scale renewable energy storage. In this study, molten carbonate fuel cell's performance was investigated with the direct conversion of methanol into syngas inside the anode chamber of the cell. The internal reforming of methanol may significantly improve system efficiency since the heat generated from the electrochemical reaction can be used directly for the endothermic reforming reaction. The porous Ni-10 wt%Cr anode was sufficient for the methanol steam reforming reaction under the fuel cell operating condition. The direct supply of methanol into the anode chamber resulted in somewhat lower cell performance, especially at high current density. Recycling of the product gas into the anode gas inlet significantly improved the cell performance. The analysis based on material balance revealed that, with increasing current density and gas recycling ratio, the methanol steam reforming reaction rate likewise increased. A methanol conversion more significant than 90% was achieved with gas recycling. The results showed the feasibility of electricity and syngas co-production using the molten carbonate fuel cell. Further research is needed to optimize the fuel cell operating conditions for simultaneous production of electricity and syngas, considering both material and energy balances in the fuel cell.

Anti-inflammatory effect and useful contents of saccharification extract powder using hot water extract from log cultivation Lentinula edodes by different UV irradiation (UV 조사시간에 따른 원목표고당화물의 유용성분 및 항염증 효과)

  • Yun, Kyeong-Won;Im, Seung-Bin;Jin, Seong-Woo;Kim, Kyung-Je;Koh, Young-Woo;Ha, Neul-I;Jeong, Hee-Gyeong;Jeong, Sang-Wook;Kim, Seung-Ju;Kim, Bok-Seon;Kim, Ki-Man;Choi, Yu-Jin;Song, Da-Hye;Seo, Kyoung-Sun
    • Journal of Mushroom
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    • v.18 no.4
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    • pp.357-364
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    • 2020
  • The grade and price of Lentinula edodes largely differs in preference depending on the product area and seasonal factors. The product amount of autumn L. edodes was higher than that of spring L. edodes, but high quality, which is divided into "Hwago" is low in preference. Mostly, the autumn L. edodes is obtained as powder; hence, it is necessary to develop a processing method to utilize its flavor and aroma at an affordable price. Additionally, we estimated the content of 𝛽-glucan, ergosterol, vitamin D2, reducing sugars, and free amino acids and evaluated the anti-inflammatory activity of saccharification powder of log-cultivated L. edodes. In the saccharification powders obtained via 7 min of UV irradiation of log-cultivated L. edodes, 𝛽-glucan and vitamin D2 contents were found to be the highest, whereas ergosterol content was found to be the lowest. The content of reducing sugars ranged from 62.4 mg/L to 68.2 mg/L. The free amino acids were higher in these saccharification powders than in the control. Subsequently, RAW 264.7 cells were treated with different concentrations (10, 50, 100, 200, 300, and 500 ㎍/mL) of the saccharification powders of log-cultivated L. edodes obtained via different UV irradiation time applications. The cells showed good viability; the anti-inflammatory effect was found to be the highest at 7 min UV irradiation. Therefore, 7 min of UV irradiation was determined to be the optimum condition for manufacturing saccharification powders of log-cultivated L. edodes. Hence, saccharification powders of log-cultivated L. edodes may be used as a raw material for natural sweeteners, food additives, and in the food industry.

Calculation of Soil Moisture and Evaporation on the Korean Peninsula using NASA LIS(Land Information System) (NASA LIS(Land Information System)을 이용한 한반도의 토양수분·증발산량 산출)

  • PARK, Gwang-Ha;YU, Wan-Sik;HWANG, Eui-Ho;JUNG, Kwan-Sue
    • Journal of the Korean Association of Geographic Information Studies
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    • v.23 no.4
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    • pp.83-100
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    • 2020
  • This study evaluated the accuracy of soil moisture and evapotranspiration by calculating the hydrological parameters in Korean peninsula using Land Information System(LIS) developed by US NASA. We used Noah-MP surface model to calculate hydrological parameters, and used MERRA2(Modern-Era Retrospective analysis for Research and Applications, Version 2) for hydrological forcing data. And, International Geosphere-Biosphere Program(IGBP) and University of Maryland(UMD) land cover maps were applied to compare the output accuracy, and Automated Synoptic Observing System(ASOS) of KMA was used as ground observation data. In order to evaluate the accuracy of the output data, the correlation coefficient(CC), BIAS, and efficiency factor (NSE, Nash-Sutcliffe Efficiency) were analyzed with soil moisture and evapotranspiration by ASOS ground observation data. As a result, the correlation coefficient of soil moisture using IGBP was 0.56 on average, and evapotranspiration was about 0.71. On the other hand, soil moisture using UMD was 0.68 on average and evapotranspiration was about 0.72, and the correlation coefficient by UMD was evaluated as high accuracy compared to the results by using IGBP. The correlation coefficient of soil moisture was an average of 0.68 and evapotranspiration was an average of 0.72 when MERRA2 was used as hydrological forcing data. On the other hand, the soil moisture applied with ASOS was an average of 0.66, and evapotranspiration was an average of 0.72. It is judged that the ASOS point data was reanalyzed as 0.65°× 0.5°grids, which is the same spatial resolution with MERRA2, resulting in differences in accuracy depending on the region.

Predicting the Pre-Harvest Sprouting Rate in Rice Using Machine Learning (기계학습을 이용한 벼 수발아율 예측)

  • Ban, Ho-Young;Jeong, Jae-Hyeok;Hwang, Woon-Ha;Lee, Hyeon-Seok;Yang, Seo-Yeong;Choi, Myong-Goo;Lee, Chung-Keun;Lee, Ji-U;Lee, Chae Young;Yun, Yeo-Tae;Han, Chae Min;Shin, Seo Ho;Lee, Seong-Tae
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.22 no.4
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    • pp.239-249
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    • 2020
  • Rice flour varieties have been developed to replace wheat, and consumption of rice flour has been encouraged. damage related to pre-harvest sprouting was occurring due to a weather disaster during the ripening period. Thus, it is necessary to develop pre-harvest sprouting rate prediction system to minimize damage for pre-harvest sprouting. Rice cultivation experiments from 20 17 to 20 19 were conducted with three rice flour varieties at six regions in Gangwon-do, Chungcheongbuk-do, and Gyeongsangbuk-do. Survey components were the heading date and pre-harvest sprouting at the harvest date. The weather data were collected daily mean temperature, relative humidity, and rainfall using Automated Synoptic Observing System (ASOS) with the same region name. Gradient Boosting Machine (GBM) which is a machine learning model, was used to predict the pre-harvest sprouting rate, and the training input variables were mean temperature, relative humidity, and total rainfall. Also, the experiment for the period from days after the heading date (DAH) to the subsequent period (DA2H) was conducted to establish the period related to pre-harvest sprouting. The data were divided into training-set and vali-set for calibration of period related to pre-harvest sprouting, and test-set for validation. The result for training-set and vali-set showed the highest score for a period of 22 DAH and 24 DA2H. The result for test-set tended to overpredict pre-harvest sprouting rate on a section smaller than 3.0 %. However, the result showed a high prediction performance (R2=0.76). Therefore, it is expected that the pre-harvest sprouting rate could be able to easily predict with weather components for a specific period using machine learning.

Ginseng Research in Natural Products Research Institute (NPRI) and the Pharmaceutical Industry Complex in Gaesong (생약연구소의 인삼연구와 약도개성)

  • Park, Ju-young
    • Journal of Ginseng Culture
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    • v.3
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    • pp.54-73
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    • 2021
  • The Natural Products Research Institute (NPRI, 生藥硏究所), an institution affiliated with Keijo Imperial University (京城帝國大學), was the predecessor of the NPRI at Seoul National University and a comprehensive research institute that focused on ginseng research during the Japanese colonial era. It was established under the leadership of Noriyuki Sugihara (杉原德行), a professor of the second lecture in pharmacology at the College of Medicine in Keijo Imperial University. Prof. Sugihara concentrated on studying Korean ginseng and herbal medicine beginning in 1926 when the second lecture of pharmacology was established. In addition to Prof. Sugihara, who majored in medicine and pharmacology, Kaku Tenmin (加來天民), an assistant professor who majored in pharmacy; Tsutomu Ishidoya (石戶谷勉), a lecturer who majored in agriculture and forestry; and about 36 researchers actively worked in the laboratory before the establishment of the NPRI in 1939. Among these personnel, approximately 14 Korean researchers had basic medical knowledge, derived mostly from specialized schools, such as medical, dental, and pharmaceutical institutions. As part of the initiative to explore the medicinal herbs of Joseon, the number of Korean researchers increased beginning in 1930. This increase started with Min Byung-Ki (閔丙祺) and Kim Ha-sik (金夏植). The second lecture of pharmacology presented various research results in areas covering medicinal plants in Joseon as well as pharmacological actions and component analyses of herbal medicines. It also conducted joint research with variousinstitutions. Meanwhile, in Gaesong (開城), the largest ginseng-producing area in Korea, the plan for the Pharmaceutical Industry Complex was established in 1935. This was a large-scale project aimed at generating profits through research on and the mass production of drugs and the reformation of the ginseng industry under collaboration among the Gaesong Ministry, Kwandong (關東) military forces, Keijo Imperial University, and private organizations. In 1936 and 1938, the Gyeonggi Provincial Medicinal Plant Research Institute (京畿道立 藥用植物硏究所) and the Herb Garden of Keijo Imperial University (京城帝國大學 藥草園) and Pharmaceutical Factory were established, respectively. These institutions merged to become Keijo Imperial University's NPRI, which wasthen overseen by Prof. Sugihara as director. Aside from conducting pharmacological research on ginseng, the NPRI devoted efforts to the development and sale of ginseng-based drugs, such as Sunryosam (鮮麗蔘), and the cultivation of ginseng. In 1941, the Jeju Urban Test Center (濟州島試驗場) was established, and an insecticide called Pancy (パンシ) was produced using Jeju-do medicinal herbs. However, even before research results were published in earnest, Japanese researchers, including Prof. Sugihara, hurriedly returned to Japan in 1945 because of the surrender of Japanese forces and the liberation of Korea. The NPRI was handed over to Seoul National University and led by Prof. Oh Jin-Sup (吳鎭燮), a former medical student at Keijo Imperial University. Scholars such as Woo Lin-Keun (禹麟根) and Seok Joo-Myung (石宙明) worked diligently to deal with the Korean pharmaceutical industry.

Cause of undeveloped primordium formation according to incubation temperature of new oyster mushroom cultivar 『Heuktari』 for bottle cultivation (병재배용 느타리 품종 『흑타리』의 배양온도에 따른 미발이 관계 규명)

  • Choi, Jong In;Kim, Jeong Han;Gwon, Hee Min;Lee, Yun Hae;Shin, Bok Eum;Gu, Ok;Ha, Tai Moon;Jung, Gu Hyun
    • Journal of Mushroom
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    • v.18 no.4
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    • pp.317-322
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    • 2020
  • This experiment was conducted to solve the failure of fruiting body production in the bottle cultivation of the oyster mushroom cultivar 'Heuktari'. The effects of incubation temperature on primordium formation and fruiting body yield of the oyster mushroom cultivar 'Heuktari' were investigated. The proper temperature for mycelium growth of 'Heuktari' on potato dextrose agar (PDA) medium is 23-26℃. The mycelial growth of 'Heuktari' was faster than that of Chunchu 2ho. During mycelial culture in sawdust medium, the temperature of the medium in the bottle initially increased, reached the highest point in the middle of the culture, and then decreased. The higher the set temperature, the shorter the incubation period. When the incubation temperatures were 20℃ and 24℃, respectively, the undeveloped primordium formation rates were low (1.8% and 4.2%, respectively). However, the rate of undeveloped primordium formation increased, and the yield decreased at incubation temperatures of 16℃ and 28℃. Mushroom farms that set incubation temperatures to 18℃ and maintained the medium temperature at less than 28℃ showed undeveloped primordium formation rates ranging between 0.3-0.8%. The rate of undeveloped primordium formation increased and the yield decreased in the farms with high incubation temperatures (above 28℃). We found that in order to reduce undeveloped primordium formation, the air inside the incubation room should be circulated continuously so that the temperature of the medium does not rise above 28℃, and dense incubation conditions should be avoided.

A Study on the Influence of Office Workers' Job Performance Ability, Retirement Readiness, and Future Anxiety on Entrepreneurship Will: Focusing on the Mediating Effect of Another Success Expectation on Life after Retirement (직장인의 직무수행능력, 노후준비도, 미래불안감이 창업의지에 미치는 영향연구: 퇴직후 삶에 대한 또 다른 성공기대감의 매개효과를 중심으로)

  • Park, Gug Gun;Ha, Kyu Soo
    • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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    • v.15 no.6
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    • pp.167-187
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    • 2020
  • Currently, Korea is changing into an ultra-aging society, and office workers retire at the age of 49.5 on average from their main jobs, and the national pension is delayed from 62 years old to 65 years old by 2034, so research is needed to prepare for the aging of office workers after retirement. The purpose of this study is to examine the factors affecting the intention to start a business after retirement and the mediating effect of another sense of success expectation on life after retirement, targeting office workers nationwide. Changes in individual attitudes and systematic institutional support are needed to prepare for a sustainable job until the age of 100 after retirement, that is, a start-up utilizing wisdom and experience in work life. As a result of the study, the ability to perform the goal as job performance, economic preparation for retirement preparation, preparation for external relations, and future anxiety have a positive effect on the entrepreneurial will, and the ability to use new technologies as job performance, and physical preparation for retirement. Preparation and preparation for internal relations were found to have no effect. In the influencing relationship between preparation for external relations and the will of start-up, and future anxiety and will of start-up, another sense of success was confirmed to have a partial mediation effect. In the relationship between economic preparation and willingness to start a business, the effect of complete mediation was confirmed. In order to increase the will to start a business after retirement, it was confirmed that another sense of expectation for success was an important variable. Introducing a government-sponsored education system in the company to reduce the government's financial burden due to super-aging and achieve corporate growth through employee training while potential founders, office workers, are employed, and entrepreneurship and goals for the three life goals of office workers By introducing a performance improvement program, we were able to get implications that would be a solution to the growth of individuals and businesses and reducing the government's financial burden.

Extract of Fructus Corni Ameliorates Testosterone-induced Benign Prostatic Hypertrophy in Sprague Dawley Rats (산수유 추출물에 의한 testosterone으로 유발된 양성 전립선 비대증의 개선)

  • Ji, Seon Yeong;Kim, Min Yeong;Hwangbo, Hyun;Lee, Hyesook;Hong, Su Hyun;Kim, Tae Hee;Yoon, Seonhye;Kim, Hyun Jin;Jung, Ha Eun;Kim, Sung Yeon;Kim, Tae Jung;Kim, Min Ji;Kim, Sung Ok;Choi, Yung Hyun
    • Journal of Life Science
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    • v.31 no.6
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    • pp.550-558
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    • 2021
  • Fructus Corni, the fruit of Cornus officinalis, has long been used for the prevention and treatment of various diseases. We recently suggested that it was effective against benign prostatic hyperplasia (BPH). In this study, we investigated the inhibitory effect of Corni Fructus (CF) water extract on BPH induced by testosterone propionate (TP) in noncastrated and castrated animal models. BPH was induced in Sprague Dawley rats by an intramuscular injection of TP in castrated or noncastrated rats. Finasteride (FINA) treatment was used as a positive control for inhibition of BPH. According to our results, CF administration inhibited excessive enlargement of development of the prostate in both the noncastrated and castrated groups compared to the control and FINA-treated groups. The inhibitory effect of CF on BPH was associated with inhibition of expression of hypoxia-inducible factor-1α, 5α-reductase type 2, steroid receptor coactivator-1, androgen receptor (AR), and prostate-specific antigen. Serum levels of the stress hormone cortisol increased during BPH induction by TP in both the noncastrated and castrated groups, but they were attenuated significantly by CF administration. However, insulin and IGF-1 levels were not increased in the BPH-induced groups and CF, and no effective results were found by CF administration. These results point to a beneficial effect of CF on BPH through inhibition of AR signaling pathway activity and imply that CF shows excellent potential as a therapeutic agent for the prevention and treatment of BPH.

Effects of Transplanting and Direct Seeding on the Growth and Yield of Rapeseed (Brassica napus L.) during Spring Cultivation (유채 봄 재배 시 기계이식과 직파 재배시기에 따른 생육 및 수량 비교)

  • Lee, Ji-Eun;Kim, Kwang-Soo;An, Da-hee;Cha, Young-Lok
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.4
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    • pp.419-427
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    • 2021
  • In South Korea, direct seeding of rapeseed (Brassica napus L.) is difficult during spring cultivation in early March because of the low yield production associated with late flowering and poor seed quality. To compare the period of direct sowing, the present study investigated the growth characteristics of rapeseed according to planting dates. Specifically, 35 day-old seedlings were transplanted or directly sown on four different dates (late February, early March, late March, and early April) in 2020 and 2021. As the planting date was delayed, the days to flowering of rapeseed decreased. Similarly, the plant height, seed set percentage, and seed yield of rapeseed were reduced upon delay in planting. The seed yield of rapeseed through direct seeding in late February was the highest, 2.76 ton·ha-1. On all seeding dates, except for late February, the transplanted rapeseed produced a higher yields than the directly seeded rapeseed. The crude oil and oleic acid content, which is related to the quality of rapeseed, decreased with the delay in planting dates, and this decrease was greater, with the direct seeding of rapeseed depending on the sowing time. In the correlation analysis, the planting date was significantly and negatively correlated with the yield, crude oil content, and oleic acid content of the transplanted rapeseed, while the sowing date was negatively correlated with the plant height, silique size, yield, and seed quality of the directly seeded rapeseed. Finally, the effect of planting date on rapeseed growth was stronger in direct seeding than in transplanting. Therefore, during spring cultivation after late February to early March, transplanting, rather than direct seeding, in more advantageous in terms of seed quality and yield.