• Title/Summary/Keyword: HVEM

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Remote Access and Data Acquisition System for High Voltage Electron Microscopy (초고전압 투과전자현미경의 원격제어 및 데이터 획득 시스템)

  • Ahn, Young-Heon;Kang, Ji-Seoun;Jung, Hyun-Joon;Kim, Hyeong-Seog;Jung, Hyung-Soo;Han, Hyuck;Jeong, Jong-Man;Gu, Jung-Eok;Lee, Sang-Dong;Lee, Jy-Soo;Cho, Kum-Won;Kim, Youn-Joong;Yeom, Heon-Young
    • Applied Microscopy
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    • v.36 no.1
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    • pp.7-16
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    • 2006

  • A new remote access system for a 1.3 MV high voltage electron microscope has been developed. Almost all essential functions for HVEM operation, huck as stage control, specimen tilting, TV camera selection and image recording, are successfully embedded into this prototype of the remote system. Particularly, this system permits perfect and precise operation of the goniometer and also controls the high resolution digital camera via simple Web browsers. Transmission of control signals and communication with the microscope is accomplished via the global ring network for advanced applications development (GLORIAD). This fact makes it possible to realize virtual laboratory to carry out practical national and international HVEM collaboration by using the present system

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3-Dimensional Reconstruction of Parallel fiber-Purkinje Cell Synapses Using High-Voltage Electron Microscopy (고압전자현미경을 이용한 소뇌 평행섬유-조롱박세포간 신경연접의 3차원 재구성)

  • Lee, Kea-Joo;Kweon, Hee-Seok;Kang, Ji-Seoun;Rhyu, Im-Joo
    • Applied Microscopy
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    • v.35 no.1
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    • pp.31-39
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    • 2005

  • Synapses are contact points where one neuron communicates with another. The morphological change of synapses under various physiological or pathological conditions has long been hypothesized to modify their functional properties. 3-dimensional (3-D) reconstruction of synapses with serial ultrathin sections has contributed to the understanding of ultrastructural dimensions and compositions of synapses. The 3-D reconstruction procedures, however, require a great amount of expertise as well as include prohibitively timeconsuming processes. Here, we introduce efficient 3-D reconstruction technique using high-voltage electron microscopy (HVEM). Primarily, we established an optimal section thickness and staining condition to observe synaptic structures in detail under HVEM. The result showed that synaptic profiles were preserved at the section thickness of 250 nm without the overlapping of synaptic ultrastructures. An increase in the reaction time of en bloc staining was most efficient to enhance contrast than the extension of postembedding staining or the addition of uranyl acetate during dehydration. Then, 3-D reconstruction of parallel fiber-Purkinje cell synapses in the rat cerebellum was carried out with serial HVEM images and reconstruction software. The images were aligned and the contours of synapses were outlined on each section. 3-D synapses were finally extracted from the section files by grouping all the synaptic contours. The reconstructed synapse model clearly demonstrated the configuration of pre and postsynaptic components. These results suggest that 3-D reconstruction of synapses using HVEM is much efficient and suitable for massive quantitative studies on synaptic connectivity than conventional TEM approach using numerous ultrathin sections.

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In-situ HRTEM Studies of Alumina-Aluminum Solid-Liquid Interfaces

  • Oh, Sang-Ho;Scheu, Christina;Ruhle, Manfred
    • Applied Microscopy
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    • v.36 no.spc1
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    • pp.19-24
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    • 2006

  • The alumina-aluminum solid-liquid interfaces were directly observed at atomic scale by heating the alumina single crystal in high-voltage electron microscope (HVEM) owing to the electron beam damage processes, Atomic ordering in the first several layers of the liquid was clearly resolved adjacent to the alumina surface and its relevance to the single crystal growth was examined with the real-time observations.

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