• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.4
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• pp.1-12
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• 2016

The Harmony Search Algorithm (HSA) is one of the recently developed metaheuristic optimization algorithms. Since the development of HSA, it has been applied by many researchers from various fields. The increasing complexity of problems has created enormous challenges for the current technique, and improved techniques of optimization algorithms are required. In this study, to improve the HSA in terms of a structural setting, a new HSA that has structural characteristics, called the Multi-layered Harmony Search Algorithm (MLHSA) was proposed. In this new method, the structural characteristics were added to HSA to improve the exploration and exploitation capability. In addition, the MLHSA was applied to optimization problems, including unconstrained benchmark functions and water distribution system pipe diameter design problems to verify the efficiency and applicability of the proposed algorithm. The results revealed the strength of MLHSA and its competitiveness.

• Journal of Korean Society of Steel Construction
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• v.29 no.1
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• pp.37-48
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• 2017

In this research, 9 SAW welding specimens with 1 hour post-heat treatment (PH1) and 2 hours (PH2) were experimented to confirm the weldability and the joint performance of 60mm thickness HSA800 steel. SAW tests with PH1 & PH2, including weld impact test, bend test, hardness test, macroscopic test, and microscopic test, had a good test results. However, from the chemical composition test results, P composition was not satisfied with Korean Industrial Standards(KS). According to the weld-zone tensile tests, 2 hours post-heat SAW is recommended because the mechanical characteristics of SAW PH2 were better than those of SAW PH1.

• Journal of Institute of Control, Robotics and Systems
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• v.17 no.8
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• pp.747-752
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• 2011

HMMs (Hidden Markov Models) are widely used for biological signal, such as EEG (electroencephalogram) sequence, analysis because of their ability to incorporate sequential information in their structure. A recent trends of research are going after the biological interpretable HMMs, and we need to control the complexity of the HMM so that it has good generalization performance. So, an automatic means of optimizing the structure of HMMs would be highly desirable. In this paper, we described a procedure of classification of motor imagery EEG signals using HMM. The motor imagery related EEG signals recorded from subjects performing left, right hand and foots motor imagery. And the proposed a method that was focus on the validation of the HSA (Harmony Search Algorithm) based optimization for HMM. Harmony search algorithm is sufficiently adaptable to allow incorporation of other techniques. A HMM training strategy using HSA is proposed, and it is tested on finding optimized structure for the pattern recognition of EEG sequence. The proposed HSA-HMM can performs global searching without initial parameter setting, local optima, and solution divergence.

• Bulletin of the Korean Chemical Society
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• v.35 no.9
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• pp.2621-2624
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• 2014

The rapid and spontaneous adsorption of proteins on nanoparticle (NP) surfaces in biological fluids such as blood is an important phenomenon as it possibly determines "what the cells see" and, thus, the fates of NPs in living organisms. In order to quantitatively understand protein coronas at the molecular level, we investigated human serum albumin (HSA) coronas that were produced on silica NPs of 20 nm and 50 nm diameters using conventional gel electrophoresis. Analysis of the concentration dependence of protein adsorption showed that HSA coronas preferentially formed a monolayer on silica NPs and revealed the presence of hard protein coronas. HSA adsorption was clearly dependent on NP size, and this might be due to the different surface curvatures of NPs of different sizes.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1994.04a
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• pp.343-343
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• 1994

1. 목 적 : 혈액 중에 존재하는 약물은 대부분 혈장 단백질과 결합하며, 비단백 결합성 약물만이 생체막을 통과하여 여러 조직에 분포되고, target eel1에서 약리학적 작용을 나타내며, 대사, 배설 될 수 있다. 단백결합율이 높은 약물일수록 비결합성 약물의 양은 적어지며, 따라서 비결합성 약물의 증가는 약효의 상승을 의미하게 된다. 최근 만성 질환에 한약의 병용투여가 증가하고 있다. 본 실험에서는 단백결합율이 높은 감초의 주성분인 Glycyrrhizic acid(GA)와 고혈압 치료제로 많이 사용되는 칼슘 길항제를 병용 투여할 경우, 칼슘 길항제의 혈장 단백결합에 미치는 영향을 살펴 보았다. 2. 방 법 : Diltiazem hydrochloride, Verapamil hydrochloride, Nifedipine 와 GA를 model 약물로 하여 평형 투석법과 한외 여과법을 이용하여 fatty acid free human serum albumin(HSA), Low density lipoprotein( LDL ), of-Acid glycoprotein(AAG), plasma 각각에 대한 결합율을 HPLC로 분석하였으며 또한 Scatchard plot를 이용하여 binding parameter를 구하였다. 3. 결과 및 고찰 : GA는 Diltiazem의 HSA와 plasma의 결합율에 영향을 미쳤으며, Verapamil의 HSA, LDL, AAG, Plasma 결합율에, 그리고 Nifedipine의 HSA, LDL, Plasma의 단백 결합율에 영향을 주었으며, 각각 n과 Ka값에 변화를 주었다.

• Microbiology and Biotechnology Letters
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• v.22 no.1
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• pp.65-72
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• 1994

Purification and characterization of pectate lyase from Bacillus sp HSA-925. Bacillus sp. HSA-925 isolated from soil produced constitutively an extracellular pectate lyase when cultivated in LB broth. The pectate lyase(EC 4.2.2.2) was purified from the cuylture broth by preciptation with ammonium sulfate, followed by column chromatography on CM-cellulose C-50 and repeated gel filtration on Sephadex G-75G. The enzyme had a molecular weight of 32-33 kDa. The activity was mazimum at pH 9.5 AND 45$\CIRC $C. The enzume activity was stable at 55$\circ $C for 15 min and between pH7-12. The activation energy, Km and V$_{max}$ for the pectate lyase were 5.8779 kcal/mol, 6.33$\times $10$^{-2}$ mol/ml and 2.09$\times $10$^{2}$ $\mu $mol/min respectively. The enzyme was activated by Ca$^{2+}$, Cu$^{2+}$ and inhibited by Li$^{+}$, Hg$^{2+}$, EDTA.

• Archives of Pharmacal Research
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• v.27 no.9
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• pp.978-983
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• 2004

The effects of glycyrrhizic acid (GLZ) on protein binding of diltiazem, verapamil, and nifedipine were investigated. Protein binding studies (human serum, human serum albumin (HSA) and (X1-acid glycoprotein (AAG)) were conducted using the equilibrium dialysis method with and without addition of GLZ. The binding parameters, such as the number of moles of bound drug per mole of protein, the number of binding sites per protein molecule, and the association con-stant, were estimated using the Scatchard plot. The serum binding of nifedipine, verapamil, and diltiazem was displaced with addition of GLZ, and the decreases of Ks for serum were observed. GLZ decreased the association constants of three drugs for HSA and AAG, while the binding capacity remained similar with addition of GLZ. Although the characteristics of interaction were not clear, GLZ seemed to mainly affect HSA binding of nifedipine rather than AAG binding, while GLZ seemed to affect both AAG- and HSA-bindings of verapamil and dilt-iazem resulting in a serum binding displacement.

• Rapid Communication in Photoscience
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• v.4 no.2
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• pp.41-44
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• 2015

Biomolecular photo-damaging activity of a water-soluble cationic porphyrin was examined using human serum albumin (HSA), a water-soluble protein as a target biomolecule model by a fluorometry. Dichlorophosphorus(V) tetraphenylporphyrin ($Cl_2P(V)TPP$), was synthesized and used as a photosensitizer. This porphyrin could bind to HSA and cause the photosensitized oxidation of HSA through the singlet oxygen generation and the oxidative photo-induced electron transfer (ET). Near infrared emission spectroscopy demonstrated the photosensitized singlet oxygen generation by this porphyrin. Decrement of the fluorescence lifetime of $Cl_2P(V)TPP$ by HSA supported the ET mechanism. Furthermore, the estimated Gibb's energy indicated that the ET mechanism is possible in the terms of energy. Because oxygen concentration in cancer cell is relatively low, ET mechanism is considered to be advantageous for photosensitizer of photodynamic therapy.

• Journal of Ocean Engineering and Technology
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• v.27 no.5
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• pp.36-42
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• 2013

We present the optimum design for the cross-sectional(sizing) and shape optimization of truss structures with natural frequency constraints. The optimum design method used in this paper employs continuous design variables and the Harmony Search Algorithm(HSA). HSA is a meta-heuristic search method for global optimization problems. In this paper, HSA uses the method of random number selection in an update process, along with penalty parameters, to construct the initial harmony memory in order to improve the fitness in the initial and update processes. In examples, 10-bar and 72-bar trusses are optimized for sizing, and 37-bar bridge type truss and 52-bar(like dome) for sizing and shape. Four typical truss optimization examples are employed to demonstrate the availability of HSA for finding the minimum weight optimum truss with multiple natural frequency constraints.

• 건축구조
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• v.21 no.6
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• pp.47-54
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• 2014